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WO1998040470A3 - Cytochrome p450 monooxygenases - Google Patents

Cytochrome p450 monooxygenases Download PDF

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Publication number
WO1998040470A3
WO1998040470A3 PCT/EP1998/001253 EP9801253W WO9840470A3 WO 1998040470 A3 WO1998040470 A3 WO 1998040470A3 EP 9801253 W EP9801253 W EP 9801253W WO 9840470 A3 WO9840470 A3 WO 9840470A3
Authority
WO
WIPO (PCT)
Prior art keywords
monooxygenases
cytochrome
nitrils
dna molecules
conversion
Prior art date
Application number
PCT/EP1998/001253
Other languages
French (fr)
Other versions
WO1998040470A2 (en
Inventor
Barbara Ann Halkier
Soeren Bak
Rachel Alice Kahn
Birger Lindberg Moeller
Original Assignee
Novartis Ag
Royal Veterinary & Agricultural Univ
Barbara Ann Halkier
Soeren Bak
Rachel Alice Kahn
Birger Lindberg Moeller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag, Royal Veterinary & Agricultural Univ, Barbara Ann Halkier, Soeren Bak, Rachel Alice Kahn, Birger Lindberg Moeller filed Critical Novartis Ag
Priority to AU67278/98A priority Critical patent/AU723454C/en
Priority to BR9808825-4A priority patent/BR9808825A/en
Priority to EP98912443A priority patent/EP0970191A2/en
Priority to PL98335643A priority patent/PL335643A1/en
Priority to KR1019997008133A priority patent/KR100583538B1/en
Priority to PCT/EP1998/001253 priority patent/WO1998040470A2/en
Priority to JP53918098A priority patent/JP2001514515A/en
Priority to US09/380,420 priority patent/US6300544B1/en
Priority to CA002282809A priority patent/CA2282809A1/en
Priority to IL13166298A priority patent/IL131662A0/en
Priority to UA99094957A priority patent/UA72185C2/en
Publication of WO1998040470A2 publication Critical patent/WO1998040470A2/en
Publication of WO1998040470A3 publication Critical patent/WO1998040470A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Cytochrome P450II dependent monooxygenases and DNA molecules encoding these monooxygenases are provided, which are able to catalyze the biosynthetic conversion of aldoximes to nitrils and the conversion of said nitrils to the corresponding cyanohydrins, which are the precursors of cyanogenic glycosides. Moreover, the invention provides methods for obtaining DNA molecules according to the invention and methods for obtaining transgenic plants resistant to insects, acarids, or nematodes or plants with improved nutritive value.
PCT/EP1998/001253 1997-03-07 1998-03-05 Cytochrome p450 monooxygenases WO1998040470A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
AU67278/98A AU723454C (en) 1997-03-07 1998-03-05 Cytochrome P450 monooxygenases
BR9808825-4A BR9808825A (en) 1997-03-07 1998-03-05 Cytochrome p450 monooxygenases
EP98912443A EP0970191A2 (en) 1997-03-07 1998-03-05 Cytochrome p450 monooxygenases
PL98335643A PL335643A1 (en) 1997-03-07 1998-03-05 P450 cytochrome mono-oxygenases
KR1019997008133A KR100583538B1 (en) 1997-03-07 1998-03-05 Cytochrome P450 Monooxygenase
PCT/EP1998/001253 WO1998040470A2 (en) 1997-03-07 1998-03-05 Cytochrome p450 monooxygenases
JP53918098A JP2001514515A (en) 1997-03-07 1998-03-05 Cytochrome P450 monooxygenase
US09/380,420 US6300544B1 (en) 1997-03-07 1998-03-05 Cytochrome P450 monooxygenases
CA002282809A CA2282809A1 (en) 1997-03-07 1998-03-05 Cytochrome p450 monooxygenases
IL13166298A IL131662A0 (en) 1997-03-07 1998-03-05 Cytochrome p450 monooxygenases
UA99094957A UA72185C2 (en) 1997-12-08 1998-05-03 Cytochrome p450 monooxygenases

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP97810132.7 1997-03-07
EP97810954.4 1997-12-08
PCT/EP1998/001253 WO1998040470A2 (en) 1997-03-07 1998-03-05 Cytochrome p450 monooxygenases

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09/380,420 A-371-Of-International US6300544B1 (en) 1997-03-07 1998-03-05 Cytochrome P450 monooxygenases
US09/899,642 Division US6649814B2 (en) 1997-03-07 2001-07-05 Cytochrome P450 monooxygenases

Publications (2)

Publication Number Publication Date
WO1998040470A2 WO1998040470A2 (en) 1998-09-17
WO1998040470A3 true WO1998040470A3 (en) 1998-12-03

Family

ID=8166889

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/001253 WO1998040470A2 (en) 1997-03-07 1998-03-05 Cytochrome p450 monooxygenases

Country Status (1)

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WO (1) WO1998040470A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1033405A3 (en) * 1999-02-25 2001-08-01 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
AU780594B2 (en) * 1999-11-12 2005-04-07 Washington State University Research Foundation Cytochrome P450 oxygenases and their uses
US6787343B2 (en) 1999-11-12 2004-09-07 Washington State University Research Foundation Cytochrome P450 oxygenases and their uses
AU772161B2 (en) * 1999-12-01 2004-04-08 Adelaide Research & Innovation Pty Ltd UDP-glucose:aglycon-glucosyltransferase
EP1246906A2 (en) * 2000-01-13 2002-10-09 Syngenta Participations AG P450 monooxygenases of the cyp79 family
GB0219723D0 (en) * 2002-08-23 2002-10-02 Syngenta Ltd Methods
EP1649005A2 (en) 2003-07-22 2006-04-26 Washington State University Research Foundation P450 oxygenases and methods of use
ZA200602938B (en) * 2003-10-16 2008-06-25 Us Smokeless Tobacco Company Cloning of cytochrome p450 genes from Nicotiana
WO2008088161A1 (en) 2007-01-19 2008-07-24 Myongji University Industry And Academia Cooperation Cytochrome p450 gene for increasing seed size or water stress resistance of plant
WO2012126059A1 (en) * 2011-03-22 2012-09-27 Monash University Novel stock feed crop plant
CN110777157B (en) * 2019-10-10 2023-07-04 广州中医药大学(广州中医药研究院) CYP450 gene for catalyzing oxidation of amyrin C-28 site, coded product and application thereof
CN113234739B (en) * 2021-05-26 2023-12-26 云南中烟工业有限责任公司 Tobacco cytochrome P450 subfamily CYP710A gene and application thereof
CN115161208B (en) * 2022-01-15 2024-03-12 云南农业大学 Saccharomyces cerevisiae genetically engineered bacterium and application thereof in production of cucurbitacin intermediate

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022441A1 (en) * 1992-04-23 1993-11-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Dna sequence of a plant resistance gene
WO1995016041A1 (en) * 1993-12-08 1995-06-15 Ciba-Geigy Ag Cytochrome p-450 monooxygenases

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022441A1 (en) * 1992-04-23 1993-11-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Dna sequence of a plant resistance gene
WO1995016041A1 (en) * 1993-12-08 1995-06-15 Ciba-Geigy Ag Cytochrome p-450 monooxygenases

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
B. KOCH ET AL.: "The biosynthesis of cyanogenic glucosides in seedlings of cassava (Manihot esculanta Crantz)", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 292, no. 1, January 1992 (1992-01-01), SAN DIEGO US, pages 141 - 150, XP002037259 *
KAHN R A ET AL: "ISOLATION AND RECONSTITUTION OF CYTOCHROME p450OX AND IN VITRO RECONSTITUTION OF THE ENTIRE BIOSYNTHETIC PATHWAY OF THE CYANOGENIC GLUCOSIDE DHURRIN FROM SORGHUM", PLANT PHYSIOLOGY (ROCKVILLE), vol. 115, no. 4, 1997, ROCKVILLE US, pages 1661 - 1670, XP002070833 *

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Publication number Publication date
WO1998040470A2 (en) 1998-09-17

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