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WO1997039722A3 - Method of isolating regulators of t cell activation - Google Patents

Method of isolating regulators of t cell activation Download PDF

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Publication number
WO1997039722A3
WO1997039722A3 PCT/US1997/007052 US9707052W WO9739722A3 WO 1997039722 A3 WO1997039722 A3 WO 1997039722A3 US 9707052 W US9707052 W US 9707052W WO 9739722 A3 WO9739722 A3 WO 9739722A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell activation
regulators
isolating
composition
primary screen
Prior art date
Application number
PCT/US1997/007052
Other languages
French (fr)
Other versions
WO1997039722A2 (en
Inventor
Augustine Y Lin
Scott W Umlauf
Andreas G Batzer
Original Assignee
T Cell Sciences Inc
Augustine Y Lin
Scott W Umlauf
Andreas G Batzer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by T Cell Sciences Inc, Augustine Y Lin, Scott W Umlauf, Andreas G Batzer filed Critical T Cell Sciences Inc
Priority to AU28148/97A priority Critical patent/AU2814897A/en
Publication of WO1997039722A2 publication Critical patent/WO1997039722A2/en
Publication of WO1997039722A3 publication Critical patent/WO1997039722A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/505—Cells of the immune system involving T-cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention provides a multi-step, high throughput primary screen to identify immune regulators of T cell activation for use as therapeutic agents. Compositions or compounds are screened for their ability to stimulate or inhibit the expression of a reporter gene operatively linked to specific transcriptional control sequences in T cells. A composition or compound identified as an immune stimulator or inhibitor by the primary screen of this invention can then be further characterized to determine the target molecule on which the composition or compound acts to regulate T cell activation and T cell activation-dependent processes such as apoptosis.
PCT/US1997/007052 1996-04-25 1997-04-25 Method of isolating regulators of t cell activation WO1997039722A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU28148/97A AU2814897A (en) 1996-04-25 1997-04-25 Method of isolating regulators of t cell activation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63928696A 1996-04-25 1996-04-25
US08/639,286 1996-04-25

Publications (2)

Publication Number Publication Date
WO1997039722A2 WO1997039722A2 (en) 1997-10-30
WO1997039722A3 true WO1997039722A3 (en) 1998-07-16

Family

ID=24563494

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/007052 WO1997039722A2 (en) 1996-04-25 1997-04-25 Method of isolating regulators of t cell activation

Country Status (2)

Country Link
AU (1) AU2814897A (en)
WO (1) WO1997039722A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7541184B2 (en) 2000-02-24 2009-06-02 Invitrogen Corporation Activation and expansion of cells
US7572631B2 (en) 2000-02-24 2009-08-11 Invitrogen Corporation Activation and expansion of T cells
US9528088B2 (en) 2002-06-28 2016-12-27 Life Technologies Corporation Methods for eliminating at least a substantial portion of a clonal antigen-specific memory T cell subpopulation

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19650714A1 (en) * 1996-12-06 1998-06-10 Melchner Harald Von Prof Dr Gene trap construct for the identification and isolation of genes
US5994507A (en) * 1997-12-31 1999-11-30 Amersham Pharmacia Biotech Ab Method for binding albumin and means to be used in the method
EP1041144B1 (en) * 1999-03-29 2004-06-23 Michael Blind Methods for the identification and validation of functional intracellular targets with intramers or in vivo selection
WO2001079555A2 (en) * 2000-04-14 2001-10-25 Millennium Pharmaceuticals, Inc. Roles of jak/stat family members in tolerance induction
AU2003275989A1 (en) * 2002-09-26 2004-04-19 Novartis Ag Screening method involving transfected primary t cells
WO2005030994A1 (en) * 2003-09-26 2005-04-07 International Institute Of Molecular And Cell Biology In Warsaw Cells and methods useful in characterising the immunotoxic activity of xenobiotic substances
US11513127B2 (en) 2016-01-25 2022-11-29 Genentech, Inc. Methods for assaying T-cell dependent bispecific antibodies

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991007487A1 (en) * 1989-11-16 1991-05-30 Duke University Particle-mediated transformation of animal tissue cells
EP0519336A2 (en) * 1991-06-18 1992-12-23 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Chimeric gene comprising a promoter-regulater and a gene coding for an indicator protein
WO1993004203A1 (en) * 1991-08-22 1993-03-04 The Board Of Trustees Of The Leland Stanford Jr. University Screening methods for immunosuppressive agents
WO1995008554A1 (en) * 1993-09-20 1995-03-30 The Board Of Trustees Of The Leland Stanford Junior University Nf-at polypeptides and polynucleotides
WO1995015173A1 (en) * 1993-11-30 1995-06-08 Lxr Biotechnology Inc. Compositions which inhibit apoptosis, methods of purifying the compositions and uses thereof
WO1995018972A1 (en) * 1994-01-05 1995-07-13 Arqule, Inc. Systematic modular production of aminimide- and oxazolone-based molecules having selected properties
WO1995027735A1 (en) * 1994-04-08 1995-10-19 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Apoptose inhibitor
WO1996035953A1 (en) * 1995-05-11 1996-11-14 Arqule, Inc. Combinatorial synthesis and high-throughput screening of a rev-inhibiting arylidenediamide array
EP0763597A2 (en) * 1995-09-14 1997-03-19 Hoechst Aktiengesellschaft Use of cytohesin-PH peptides to influence the adhesion capability of integrins
WO1997040022A1 (en) * 1996-04-25 1997-10-30 T Cell Sciences, Inc. Immunosuppressive compounds from an arylidene diamide array

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991007487A1 (en) * 1989-11-16 1991-05-30 Duke University Particle-mediated transformation of animal tissue cells
EP0519336A2 (en) * 1991-06-18 1992-12-23 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Chimeric gene comprising a promoter-regulater and a gene coding for an indicator protein
WO1993004203A1 (en) * 1991-08-22 1993-03-04 The Board Of Trustees Of The Leland Stanford Jr. University Screening methods for immunosuppressive agents
WO1995008554A1 (en) * 1993-09-20 1995-03-30 The Board Of Trustees Of The Leland Stanford Junior University Nf-at polypeptides and polynucleotides
WO1995015173A1 (en) * 1993-11-30 1995-06-08 Lxr Biotechnology Inc. Compositions which inhibit apoptosis, methods of purifying the compositions and uses thereof
WO1995018972A1 (en) * 1994-01-05 1995-07-13 Arqule, Inc. Systematic modular production of aminimide- and oxazolone-based molecules having selected properties
WO1995027735A1 (en) * 1994-04-08 1995-10-19 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Apoptose inhibitor
WO1996035953A1 (en) * 1995-05-11 1996-11-14 Arqule, Inc. Combinatorial synthesis and high-throughput screening of a rev-inhibiting arylidenediamide array
EP0763597A2 (en) * 1995-09-14 1997-03-19 Hoechst Aktiengesellschaft Use of cytohesin-PH peptides to influence the adhesion capability of integrins
WO1997040022A1 (en) * 1996-04-25 1997-10-30 T Cell Sciences, Inc. Immunosuppressive compounds from an arylidene diamide array

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DHEIN J ET AL: "AUTOCRINE T-CELL SUICIDE MEDIATED BY APO-1/(FAS/CD95)", NATURE, vol. 373, 2 February 1995 (1995-02-02), pages 438 - 441, XP002035056 *
JEITNER T M ET AL.: "Thiol-bearing compounds selectively inhibit protein kinase C-dependent events and proliferation in human T cells", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1223, no. 1, 1994, pages 15 - 22, XP002063832 *
NORTHROP J P ET AL: "CHARACTERIZATION OF THE NUCLEAR AND CYTOPLASMIC COMPONENTS OF THE LYMPHOID-SPECIFIC NUCLEAR FACTOR OF ACTIVATED T CELLS (NF-AT) COMPLEX", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 4, 5 February 1993 (1993-02-05), pages 2917 - 2923, XP002053814 *
SU B ET AL: "JNK IS INVOLVED IN SIGNAL INTEGRATION DURING COSTIMULATION OF T-LYMHOCYTES", CELL, vol. 77, 3 June 1994 (1994-06-03), pages 727 - 736, XP002053843 *
UMLAUF S A ET AL: "DISCOVERY OF NOVEL IMMUNOMODULATORY COMPOUNDS BY FUNCTIONAL SCREENING OF A SYNTHETIC ARRAY LIBRARY", FASEB JOURNAL, vol. 10, no. 6, 30 April 1996 (1996-04-30), pages A1453, XP002053816 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7541184B2 (en) 2000-02-24 2009-06-02 Invitrogen Corporation Activation and expansion of cells
US7572631B2 (en) 2000-02-24 2009-08-11 Invitrogen Corporation Activation and expansion of T cells
US9528088B2 (en) 2002-06-28 2016-12-27 Life Technologies Corporation Methods for eliminating at least a substantial portion of a clonal antigen-specific memory T cell subpopulation

Also Published As

Publication number Publication date
AU2814897A (en) 1997-11-12
WO1997039722A2 (en) 1997-10-30

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