+

WO1997032040A3 - Nucleic acid sequence detection - Google Patents

Nucleic acid sequence detection Download PDF

Info

Publication number
WO1997032040A3
WO1997032040A3 PCT/GB1997/000548 GB9700548W WO9732040A3 WO 1997032040 A3 WO1997032040 A3 WO 1997032040A3 GB 9700548 W GB9700548 W GB 9700548W WO 9732040 A3 WO9732040 A3 WO 9732040A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid sequence
strand
predetermined
end region
Prior art date
Application number
PCT/GB1997/000548
Other languages
French (fr)
Other versions
WO1997032040A2 (en
Inventor
David Stirling
Christopher Armstrong Ludlam
Original Assignee
Royal Infirmary Of Edinburgh N
David Stirling
Christopher Armstrong Ludlam
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Royal Infirmary Of Edinburgh N, David Stirling, Christopher Armstrong Ludlam filed Critical Royal Infirmary Of Edinburgh N
Priority to AU18897/97A priority Critical patent/AU1889797A/en
Publication of WO1997032040A2 publication Critical patent/WO1997032040A2/en
Publication of WO1997032040A3 publication Critical patent/WO1997032040A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a detection system suitable for use in a method of detecting the presence or absence of a predetermined nucleic acid sequence in a sample of nucleic acid. The system comprises a first oligonucleotide primer capable of annealing with a first strand of nucleic acid containing said predetermined nucleic acid sequence, such that a 3' end region of the first primer anneals with a first portion of said predetermined nucleic acid sequence; a second oligonucleotide primer having a 3' end region capable of annealing with a second strand of nucleic acid complementary to said first strand of nucleic acid such that a 3' end region of the second primer anneals with a portion of said second strand which portion is complementary to a second portion of said predetermined nucleic acid sequence, which second portion is spaced from end 5' of said first portion. One of the first and the second primers is labelled and the other is anchored to a substrate. Upon subjecting a sample of nucleic acid to an amplification reaction in the presence of said first and second oligonucleotide primers the presence of said predetermined nucleic acid sequence is indicated by the production of an amplified labelled nucleic acid product captively retained on said substrate.
PCT/GB1997/000548 1996-02-29 1997-02-27 Nucleic acid sequence detection WO1997032040A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU18897/97A AU1889797A (en) 1996-02-29 1997-02-27 Nucleic acid sequence detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9604267.6 1996-02-29
GBGB9604267.6A GB9604267D0 (en) 1996-02-29 1996-02-29 Mutation assay

Publications (2)

Publication Number Publication Date
WO1997032040A2 WO1997032040A2 (en) 1997-09-04
WO1997032040A3 true WO1997032040A3 (en) 1997-10-09

Family

ID=10789590

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1997/000548 WO1997032040A2 (en) 1996-02-29 1997-02-27 Nucleic acid sequence detection

Country Status (3)

Country Link
AU (1) AU1889797A (en)
GB (1) GB9604267D0 (en)
WO (1) WO1997032040A2 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7351557B2 (en) 2001-03-09 2008-04-01 Nugen Technologies, Inc. Amplification of RNA sequences using composite RNA-DNA primers and strand displacement
US7402386B2 (en) 2003-04-14 2008-07-22 Nugen Technologies, Inc. Global amplification using random priming by a composite primer
US7771946B2 (en) 2001-03-09 2010-08-10 Nugen Technologies, Inc. Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences
US7771934B2 (en) 2000-12-13 2010-08-10 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
DE19738816A1 (en) * 1997-09-05 1999-03-11 November Ag Molekulare Medizin Method for marking solid, liquid or gaseous substances
DE19811731A1 (en) 1998-03-18 1999-09-23 November Ag Molekulare Medizin Detecting nucleic acid by amplification, with use of third, immobilized primer
DE19840897A1 (en) * 1998-09-08 2000-03-09 Dirk Happich Assay for detecting the factor V disease mutation comprises a polymerase chain reaction using a reporter/quencher-labeled probe having a defined nucleotide sequence
US6638719B1 (en) * 1999-07-14 2003-10-28 Affymetrix, Inc. Genotyping biallelic markers
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7250252B2 (en) 1999-12-30 2007-07-31 David Aaron Katz Amplification based polymorphism detection
CN1498274A (en) 2000-06-26 2004-05-19 Ŧب������˾ Methods and compositions for transcription-based nuleic acid amplification
SG90149A1 (en) * 2000-07-18 2002-07-23 Government Of The Republic Of Diagnostic assay
DE50012114D1 (en) * 2000-09-05 2006-04-13 Zeltz Patrick Method for the specific determination of DNA sequences by means of parallel amplification
US20030022175A1 (en) * 2001-07-26 2003-01-30 Harn-Jing Terng Diagnostic assay of genetic mutations by discriminating amplification and hybridization
FR2866349B1 (en) * 2004-02-12 2008-05-16 Biomerieux Sa METHOD FOR DIAGNOSING / PROGNOSING THROMBOSIS

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990011369A1 (en) * 1989-03-22 1990-10-04 Cemu Bioteknik Ab Solid phase diagnosis of medical conditions
EP0521318A2 (en) * 1991-06-10 1993-01-07 Lucky Ltd. Hepatitis C diagnostics and vaccines
WO1993009250A1 (en) * 1991-11-01 1993-05-13 Adelaide Children's Hospital Solid phase amplification process
JPH07184695A (en) * 1993-12-27 1995-07-25 Sanwa Kagaku Kenkyusho Co Ltd Method for simply detecting hepatitis c virus
WO1995021938A1 (en) * 1994-02-14 1995-08-17 Rijks Universiteit Leiden A method for screening for the presence of a genetic defect associated with thrombosis and/or poor anticoagulant response to activated protein c
WO1995029259A1 (en) * 1994-04-22 1995-11-02 Stichting Centraal Laboratorium Van De Bloedtransfusiedienst Van Het Nederlandse Rode Kruis Method and means for detecting and treating disorders in the blood coagulation cascade

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990011369A1 (en) * 1989-03-22 1990-10-04 Cemu Bioteknik Ab Solid phase diagnosis of medical conditions
EP0521318A2 (en) * 1991-06-10 1993-01-07 Lucky Ltd. Hepatitis C diagnostics and vaccines
WO1993009250A1 (en) * 1991-11-01 1993-05-13 Adelaide Children's Hospital Solid phase amplification process
JPH07184695A (en) * 1993-12-27 1995-07-25 Sanwa Kagaku Kenkyusho Co Ltd Method for simply detecting hepatitis c virus
WO1995021938A1 (en) * 1994-02-14 1995-08-17 Rijks Universiteit Leiden A method for screening for the presence of a genetic defect associated with thrombosis and/or poor anticoagulant response to activated protein c
WO1995029259A1 (en) * 1994-04-22 1995-11-02 Stichting Centraal Laboratorium Van De Bloedtransfusiedienst Van Het Nederlandse Rode Kruis Method and means for detecting and treating disorders in the blood coagulation cascade

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 095, no. 010 30 November 1995 (1995-11-30) *
POORT ET AL.: "A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis", BLOOD, vol. 88, no. 10, 15 November 1996 (1996-11-15), pages 3698 - 3703, XP002037583 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US8334116B2 (en) 2000-12-13 2012-12-18 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US7771934B2 (en) 2000-12-13 2010-08-10 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US7771946B2 (en) 2001-03-09 2010-08-10 Nugen Technologies, Inc. Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences
US8071311B2 (en) 2001-03-09 2011-12-06 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
US7351557B2 (en) 2001-03-09 2008-04-01 Nugen Technologies, Inc. Amplification of RNA sequences using composite RNA-DNA primers and strand displacement
US9181582B2 (en) 2001-03-09 2015-11-10 Nugen Technologies, Inc. Compositions for amplification of RNA sequences using composite primers
US7402386B2 (en) 2003-04-14 2008-07-22 Nugen Technologies, Inc. Global amplification using random priming by a composite primer
US8465950B2 (en) 2003-04-14 2013-06-18 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US9175325B2 (en) 2003-04-14 2015-11-03 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA

Also Published As

Publication number Publication date
GB9604267D0 (en) 1996-05-01
AU1889797A (en) 1997-09-16
WO1997032040A2 (en) 1997-09-04

Similar Documents

Publication Publication Date Title
WO1997032040A3 (en) Nucleic acid sequence detection
US4683202B1 (en)
EP0875584A3 (en) Nucleic acid assays
GB9210176D0 (en) Chemical method
EP1757694A3 (en) Prostate-specific membrane antigen
GB9016163D0 (en) Chemical method
CA2067909A1 (en) Homogeneous methods for nucleic acid amplification and detection
AU1250799A (en) Pcr primer constructs for use in an integrated signalling system
WO2003020891A3 (en) Single nucleotide amplification and detection by polymerase
WO1999060159A3 (en) Method for using unequal primer concentrations for generating nucleic acid amplification products
IL102765A (en) Reagents and methods to detect and determine mycobacterium nucleic acids
WO1994003630A3 (en) Method, reagent and kit for the detection and amplification of nucleic acid sequences
WO2002061133A3 (en) Nucleic acid detection using structured probes
AR001619A1 (en) Method for amplification of specific nucleic acid sequences
CA2164941A1 (en) Salmonella identification by the polymerase chain reaction
BR9606602A (en) Oligonucleotide amplification primer and method for complex complex amplification of an acid
WO1999007896A3 (en) Detection and confirmation of nucleic acid sequences by use of oligonucleotides comprising a subsequence hybridizing exactly to a known terminal sequence and a subsequence hybridizing to an unidentified sequence
WO1996015264A3 (en) Method for identifying nucleic acid sequences from different biological sources through amplification of the same
WO1998024928A3 (en) Detection of chromosomal abnormalities
EP0795612A3 (en) Amplifying and detecting target nucleic acids using a post amplification incubation step
EP0682121A4 (en) Method of amplifying and detecting target nucleic acid sequence by using thermostable enzymes.
WO2002014534A3 (en) Method of amplifying nucleic acids providing means for strand separation and universal sequence tags
WO2002079512A3 (en) Method for detection and quantification of the fungus aspergillus fumigatus using quantitative pcr
GB9918237D0 (en) Detection system
WO1997028279A3 (en) Positive controls for use in polynucleotide amplification

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN YU AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN YU AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: JP

Ref document number: 97530707

Format of ref document f/p: F

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA

122 Ep: pct application non-entry in european phase
点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载