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WO1997020058A3 - PLANT GENE EXPRESSION VECTOR FAMILY BASED ON THE REGULATORY DNA SEQUENCES OF AN ALFALFA H3 HISTONE GENE VARIANT (MsH3g1) - Google Patents

PLANT GENE EXPRESSION VECTOR FAMILY BASED ON THE REGULATORY DNA SEQUENCES OF AN ALFALFA H3 HISTONE GENE VARIANT (MsH3g1) Download PDF

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Publication number
WO1997020058A3
WO1997020058A3 PCT/HU1996/000070 HU9600070W WO9720058A3 WO 1997020058 A3 WO1997020058 A3 WO 1997020058A3 HU 9600070 W HU9600070 W HU 9600070W WO 9720058 A3 WO9720058 A3 WO 9720058A3
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WO
WIPO (PCT)
Prior art keywords
msh3g1
alfalfa
expression vector
dna sequences
regulatory dna
Prior art date
Application number
PCT/HU1996/000070
Other languages
French (fr)
Other versions
WO1997020058A2 (en
Inventor
Tamas Kapros
Denes Dudits
Janos Gyoergyey
Antal Mai
Zsolt Kelemen
Original Assignee
Bay Zoltan Alkalmazott Kutatas
Mta Szegedi Biolog Koezpont No
Tamas Kapros
Denes Dudits
Janos Gyoergyey
Antal Mai
Zsolt Kelemen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bay Zoltan Alkalmazott Kutatas, Mta Szegedi Biolog Koezpont No, Tamas Kapros, Denes Dudits, Janos Gyoergyey, Antal Mai, Zsolt Kelemen filed Critical Bay Zoltan Alkalmazott Kutatas
Priority to AU77052/96A priority Critical patent/AU7705296A/en
Publication of WO1997020058A2 publication Critical patent/WO1997020058A2/en
Publication of WO1997020058A3 publication Critical patent/WO1997020058A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Disclosed herein are nucleic acid molecules comprising sequence regions participating in the regulation of the expression of the H3g1 gene of alfalfa (Medicago sativa). A plant gene expression vector family based on the regulatory DNA sequences of said histone gene variant (MsH3g1) is also disclosed. The invention also provides transformed cells, transgenic plants and parts thereof comprising the nucleic acid sequences of the invention.
PCT/HU1996/000070 1995-11-24 1996-11-22 PLANT GENE EXPRESSION VECTOR FAMILY BASED ON THE REGULATORY DNA SEQUENCES OF AN ALFALFA H3 HISTONE GENE VARIANT (MsH3g1) WO1997020058A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU77052/96A AU7705296A (en) 1995-11-24 1996-11-22 Plant gene expression vector family based on the regulatory dna sequences of an alfalfa h3 histon gene variant (msh3g1)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU9503352A HUT76355A (en) 1995-11-24 1995-11-24 Plant gene-expression vector family based on dna fragments regulating alfalfa h3 histon gene variant (ms h3g1)
HUP9503352 1995-11-24

Publications (2)

Publication Number Publication Date
WO1997020058A2 WO1997020058A2 (en) 1997-06-05
WO1997020058A3 true WO1997020058A3 (en) 1997-07-17

Family

ID=10987390

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/HU1996/000070 WO1997020058A2 (en) 1995-11-24 1996-11-22 PLANT GENE EXPRESSION VECTOR FAMILY BASED ON THE REGULATORY DNA SEQUENCES OF AN ALFALFA H3 HISTONE GENE VARIANT (MsH3g1)

Country Status (3)

Country Link
AU (1) AU7705296A (en)
HU (1) HUT76355A (en)
WO (1) WO1997020058A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9923306D0 (en) 1999-10-01 1999-12-08 Isis Innovation Diagnostic and therapeutic epitope, and transgenic plant
US7122721B1 (en) 1999-10-05 2006-10-17 Basf Aktiengesellschaft Plant gene expression under the control of constitutive plant V-ATPase promoters
GB0212885D0 (en) 2002-06-05 2002-07-17 Isis Innovation Therapeutic epitopes and uses thereof
EP2412380B1 (en) 2004-04-28 2021-01-06 BTG International Limited Epitopes related to coeliac disease
US10105437B2 (en) 2004-04-28 2018-10-23 Btg International Limited Epitopes related to coeliac disease

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0342926A2 (en) * 1988-05-17 1989-11-23 Mycogen Plant Science, Inc. Plant ubiquitin promoter system
EP0507698A1 (en) * 1991-03-05 1992-10-07 Rhone-Poulenc Agrochimie Histone promotors
EP0633317A1 (en) * 1993-06-25 1995-01-11 Rhone-Poulenc Agrochimie Isolated DNA sequence able to function as a termination region in chimeric genes for plant transformation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0342926A2 (en) * 1988-05-17 1989-11-23 Mycogen Plant Science, Inc. Plant ubiquitin promoter system
EP0507698A1 (en) * 1991-03-05 1992-10-07 Rhone-Poulenc Agrochimie Histone promotors
EP0633317A1 (en) * 1993-06-25 1995-01-11 Rhone-Poulenc Agrochimie Isolated DNA sequence able to function as a termination region in chimeric genes for plant transformation

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
ATANASSOVA, ROSSITZA ET AL: "A 126 bp fragment of a plant histone gene promoter confers preferential expression in meristems of transgenic Arabidopsis", PLANT J. (1992), 2(3), 291-300, XP002030436 *
CHAUBET N ET AL: "GENES ENCODING A HISTONE H3.3-LIKE VARIANT IN ARABIDOPSIS CONTAIN INTERVENING SEQUENCES", JOURNAL OF MOLECULAR BIOLOGY, vol. 225, 1992, pages 569 - 574, XP002023308 *
KAPROS T ET AL: "A SHORT HISTONE H-3 PROMOTER FROM ALFALFA SPECIFIES EXPRESSION IN S-PHASE CELLS AND MERISTEMS.", IN VITRO CELL DEV BIOL PLANT 29P (1). 1993. 27-32., XP000671641 *
KAPROS T ET AL: "DIFFERENTIAL EXPRESSION OF HISTONE H3 GENE VARIANTS DURING CELL CYCLE AND SOMATIC EMBRYOGENESIS IN ALFALFA.", PLANT PHYSIOL (BETHESDA) 98 (2). 1992. 621-625., XP002030434 *
LEPETIT M ET AL: "A PLANT HISTONE GENE PROMOTER CAN DIRECT BOTH REPLICATION-DEPENDENT AND INDEPENDENT GENE EXPRESSION IN TRANSGENIC PLANTS.", MOL GEN GENET 231 (2). 1992. 276-285., XP002030435 *
OMIRULLEH, SERIK ET AL: "Differential activity of wheat histone H4 promoter in transgenic maize", NATO ASI SER., SER. H (1994), 81(PLANT MOLECULAR BIOLOGY), 549-58, XP000671634 *
ROBERTSON, A.J.: "Medicago sativa cultivar Chief histone H3.2 gene 5' region and complete cds", EMBL SEQUENCE DATABASE, REL. 39, 26-MAY-1994, ACCESSION NO. U09458, XP002030433 *
SEILER-TUYNS A ET AL: "A CHIMERIC MOUSE HISTONE H4 GENE CONTAINING EITHER AN INTRON OR POLY(A)ADDITION SIGNAL BEHAVES LIKE A BASAL HISTONE", NUCLEIC ACIDS RESEARCH, vol. 14, no. 22, 1986, pages 8845 - 8862, XP002023311 *
SINIBALDI R M ET AL: "INTRON SPLICING AND INTRON-MEDIATED ENHANCED EXPRESSION IN MONOCOTS", PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, vol. 42, 1992, pages 229 - 257, XP002023310 *
TANAKA A ET AL: "ENHANCEMENT OF FOREIGN GENE EXPRESSION BY A DICOT INTRON IN RICE BUT NOT IN TOBACCO IS CORRELATED WITH AN INCREASED LEVEL OF MRNA AND AN EFFICIENT SPLICING OF THE INTRON", NUCLEIC ACIDS RESEARCH, vol. 18, no. 23, 1990, pages 6767 - 6770, XP002023309 *
TERADA, R., ET AL.: "A wheat histone H3 promoter confers cell division-dependent and -independent expression of a gus A gene in transgenic rice plants", THE PLANT JOURNAL, vol. 4, 1993, pages 241 - 252, XP002030453 *

Also Published As

Publication number Publication date
AU7705296A (en) 1997-06-19
HUT76355A (en) 1997-08-28
HU9503352D0 (en) 1996-02-28
WO1997020058A2 (en) 1997-06-05

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