WO1997014797A3 - Cystatin m, a novel cysteine proteinase inhibitor - Google Patents
Cystatin m, a novel cysteine proteinase inhibitor Download PDFInfo
- Publication number
- WO1997014797A3 WO1997014797A3 PCT/US1996/016782 US9616782W WO9714797A3 WO 1997014797 A3 WO1997014797 A3 WO 1997014797A3 US 9616782 W US9616782 W US 9616782W WO 9714797 A3 WO9714797 A3 WO 9714797A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cystatin
- nucleic acid
- cysteine proteinase
- acid molecules
- proteinase inhibitor
- Prior art date
Links
- 102000015833 Cystatin Human genes 0.000 title abstract 2
- 102100028007 Cystatin-SA Human genes 0.000 title abstract 2
- 101710144510 Cysteine proteinase inhibitor Proteins 0.000 title abstract 2
- 108050004038 cystatin Proteins 0.000 title abstract 2
- 239000002852 cysteine proteinase inhibitor Substances 0.000 title abstract 2
- 108010061617 Cystatin M Proteins 0.000 abstract 4
- 102000012177 Cystatin M Human genes 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 2
- 239000013604 expression vector Substances 0.000 abstract 2
- 210000004881 tumor cell Anatomy 0.000 abstract 2
- 108010005843 Cysteine Proteases Proteins 0.000 abstract 1
- 102000005927 Cysteine Proteases Human genes 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 108090000526 Papain Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 230000000692 anti-sense effect Effects 0.000 abstract 1
- 230000000890 antigenic effect Effects 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000001394 metastastic effect Effects 0.000 abstract 1
- 206010061289 metastatic neoplasm Diseases 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 235000019834 papain Nutrition 0.000 abstract 1
- 229940055729 papain Drugs 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 238000003259 recombinant expression Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 238000002560 therapeutic procedure Methods 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Peptides Or Proteins (AREA)
Abstract
Isolated nucleic acid molecules encoding a novel cysteine proteinase inhibitor, cystatin M, which is a member of the Family 2 cystatins, are disclosed. Cystatin M exhibits cysteine proteinase inhibitory activity against papain, is downregulated in metastatic mammary epithelial tumor cells, as well as other tumor cells, and is upregulated in senescent cells. In addition to isolated nucleic acid molecules, the invention provides antisense nucleic acid molecules, recombinant expression vectors containing a nucelic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a cystatin M gene has been introduced or disrupted. The invention further provides isolated cystatin M proteins, fusion proteins, antigenic peptides and anti-cystatin M antibodies. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU74579/96A AU7457996A (en) | 1995-10-20 | 1996-10-18 | Cystatin m, a novel cysteine proteinase inhibitor |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54600095A | 1995-10-20 | 1995-10-20 | |
| US08/546,000 | 1995-10-20 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO1997014797A2 WO1997014797A2 (en) | 1997-04-24 |
| WO1997014797A3 true WO1997014797A3 (en) | 1997-10-02 |
| WO1997014797A9 WO1997014797A9 (en) | 1997-10-30 |
Family
ID=24178431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/016782 WO1997014797A2 (en) | 1995-10-20 | 1996-10-18 | Cystatin m, a novel cysteine proteinase inhibitor |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU7457996A (en) |
| WO (1) | WO1997014797A2 (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6171790B1 (en) * | 1998-05-01 | 2001-01-09 | Incyte Pharmaceuticals, Inc. | Human protease associated proteins |
| US6001596A (en) * | 1998-05-07 | 1999-12-14 | Incyte Pharmaceuticals, Inc. | Growth-associated protease inhibitor heavy chain precursor |
| JP4592127B2 (en) * | 1999-03-30 | 2010-12-01 | 雪印乳業株式会社 | Bone resorption inhibitor |
| US6366762B1 (en) * | 1999-03-31 | 2002-04-02 | Qualcomm, Inc. | System and method for measuring round trip delay on the paging and access channels |
| CA2374745A1 (en) * | 1999-08-09 | 2001-02-15 | Incyte Genomics, Inc. | Proteases and protease inhibitors |
| US6943277B2 (en) | 2000-07-11 | 2005-09-13 | Deltagen, Inc. | Transgenic mice containing stefin homolog protease inhibitor gene disruptions |
| AU2001273352A1 (en) * | 2000-07-11 | 2002-01-21 | Deltagen, Inc. | Transgenic mice containing targeted protease inhibitor gene disruptions |
| AU2003202689A1 (en) | 2002-01-23 | 2003-09-09 | El-Gewely, Mohamed, Raafat | Methods of screening molecular libraries for active molecules that restore the function of target proteins and peptides that restore the function of mutant p53 |
| CN112379093B (en) * | 2020-10-22 | 2023-06-16 | 上海良润生物医药科技有限公司 | Application of CST-Cathepsin complex as tumor diagnosis marker |
| CN113957077B (en) * | 2021-10-18 | 2023-05-16 | 中国热带农业科学院环境与植物保护研究所 | Sisal hemp cysteine proteinase inhibitor gene and application thereof |
| CN116023472A (en) * | 2023-02-20 | 2023-04-28 | 大连工业大学 | Method for producing cysteine protease inhibitor by high-efficiency expression |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3724581A1 (en) * | 1987-07-24 | 1989-02-02 | Gruenenthal Gmbh | DNA SEQUENCES COATING FOR PROTEINS WITH THE BIOLOGICAL PROPERTIES OF CYSTATINE C, PREPARATION OF THESE DNA SEQUENCES, EXPRESSION OF CYSTATINE C, AND PHARMACEUTICAL PREPARATIONS CONTAINING THEREOF |
| JPH07126294A (en) * | 1993-04-28 | 1995-05-16 | Snow Brand Milk Prod Co Ltd | New cysteine protease inhibitor |
-
1996
- 1996-10-18 AU AU74579/96A patent/AU7457996A/en not_active Abandoned
- 1996-10-18 WO PCT/US1996/016782 patent/WO1997014797A2/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3724581A1 (en) * | 1987-07-24 | 1989-02-02 | Gruenenthal Gmbh | DNA SEQUENCES COATING FOR PROTEINS WITH THE BIOLOGICAL PROPERTIES OF CYSTATINE C, PREPARATION OF THESE DNA SEQUENCES, EXPRESSION OF CYSTATINE C, AND PHARMACEUTICAL PREPARATIONS CONTAINING THEREOF |
| JPH07126294A (en) * | 1993-04-28 | 1995-05-16 | Snow Brand Milk Prod Co Ltd | New cysteine protease inhibitor |
Non-Patent Citations (3)
| Title |
|---|
| DATABASE WPI Week 9528, Derwent World Patents Index; AN 95-212959, XP002031097 * |
| EMBL DATABASE, Accession no U62800. 26 Aug1996. SOTIROPOULOU G. et al: "Identification, cloning and characterization of cystatin M...." * |
| SOTIROPOULOU G ET AL: "Isolation of a novel protease inhibitor involved in breast cancer and senescence.", 1ST WORLD CONGRESS ON ADVANCES IN ONCOLOGY, ATHENS, GREECE, OCTOBER 22-26, 1995. INTERNATIONAL JOURNAL OF ONCOLOGY 7 (SUPPL.). 1995. 969. ISSN: 1019-6439, XP000672818 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1997014797A2 (en) | 1997-04-24 |
| AU7457996A (en) | 1997-05-07 |
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