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WO1997013853A3 - Protein detection - Google Patents

Protein detection Download PDF

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Publication number
WO1997013853A3
WO1997013853A3 PCT/EP1996/004510 EP9604510W WO9713853A3 WO 1997013853 A3 WO1997013853 A3 WO 1997013853A3 EP 9604510 W EP9604510 W EP 9604510W WO 9713853 A3 WO9713853 A3 WO 9713853A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
eukaryotic organism
screened
host cells
dna
Prior art date
Application number
PCT/EP1996/004510
Other languages
French (fr)
Other versions
WO1997013853A2 (en
Inventor
Den Broeck Henriette Catha Van
Graaff Leendert Hendrik De
Jacob Visser
Ooyen Albert Johannes Jose Van
Original Assignee
Gist Brocades Bv
Den Broeck Henriette Catharina
Graaff Leendert Hendrik De
Jacob Visser
Ooyen Albert Johannes Joseph V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Bv, Den Broeck Henriette Catharina, Graaff Leendert Hendrik De, Jacob Visser, Ooyen Albert Johannes Joseph V filed Critical Gist Brocades Bv
Priority to EP96934722A priority Critical patent/EP0796328A2/en
Priority to JP9514730A priority patent/JPH10510720A/en
Priority to AU72943/96A priority patent/AU7294396A/en
Publication of WO1997013853A2 publication Critical patent/WO1997013853A2/en
Publication of WO1997013853A3 publication Critical patent/WO1997013853A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055—Alpha-N-arabinofuranosidase (3.2.1.55)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a method for identifying a DNA fragment encoding a protein of interest, wherein a cDNA library in the form of bacterial host cells transformed with DNA obtainable from an eukaryotic organism capable of producing said protein is screened for expression of said protein using a test which is indicative for its presence, characterised in that screening of the host cells is performed after the transforming DNA has become part of a plasmid. The cDNA library is preferably from an eukaryotic organism. The eukaryotic organism is preferably a fungus or a yeast cell, more preferably an Aspergillus species, most preferred is a member of the Aspergillus niger group. The enzymes screened for are preferably enzymes which show cellulase, endoxylanase or arabinoxylan degrading activity.
PCT/EP1996/004510 1995-10-13 1996-10-14 Protein detection WO1997013853A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP96934722A EP0796328A2 (en) 1995-10-13 1996-10-14 Protein detection
JP9514730A JPH10510720A (en) 1995-10-13 1996-10-14 Protein detection method
AU72943/96A AU7294396A (en) 1995-10-13 1996-10-14 Protein detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP95202777.9 1995-10-13
EP95202777 1995-10-13

Publications (2)

Publication Number Publication Date
WO1997013853A2 WO1997013853A2 (en) 1997-04-17
WO1997013853A3 true WO1997013853A3 (en) 1997-06-19

Family

ID=8220719

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1996/004510 WO1997013853A2 (en) 1995-10-13 1996-10-14 Protein detection

Country Status (4)

Country Link
EP (1) EP0796328A2 (en)
JP (1) JPH10510720A (en)
AU (1) AU7294396A (en)
WO (1) WO1997013853A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
EP0976838A1 (en) 1998-05-06 2000-02-02 Rhone-Poulenc Nutrition Animale Enzymes mixture
EA005682B1 (en) 1998-10-06 2005-04-28 Марк Аарон Эмалфарб Transformed fungal strain of the genius chrysosporium
FR2786784B1 (en) * 1998-12-04 2003-01-24 Lesaffre & Cie DNA FRAGMENT ENCODING THERMOASCUS XynA THERMOSTABLE XYLANASE
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
MXPA02010155A (en) 2000-04-13 2004-08-19 Mark Aaron Emalfarb Expression-regulating sequences and expression products in the field of filamentous fungi chrysosporium.
WO2003106654A2 (en) 2002-06-14 2003-12-24 Diversa Corporation Xylanases, nucleic adics encoding them and methods for making and using them
CA2915553A1 (en) * 2003-05-29 2005-01-06 Genencor International, Inc. Trichoderma genes
FI120045B (en) 2005-12-22 2009-06-15 Roal Oy Treatment of cellulose materials and enzymes useful therein
USRE45660E1 (en) 2006-02-14 2015-09-01 Bp Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
US20100040595A1 (en) 2006-09-29 2010-02-18 Novozymes A/S Xylanses for animal feed
CN103757036B (en) 2007-10-03 2019-10-01 维莱尼姆公司 Zytase, the nucleic acid and its methods for making and using same for encoding them
CN102414323B (en) * 2009-04-22 2015-07-08 帝斯曼知识产权资产管理有限公司 Process for production of recombinant polypeptide of interest
US9012186B2 (en) 2009-04-27 2015-04-21 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
EA201391232A1 (en) * 2011-02-25 2014-02-28 Юниверсити Оф Саскачеван PROTEINS FOR BIOREGULATION OF PATHOGENIC FOR PLANTS OF FUNGI
CN106434246A (en) 2011-09-14 2017-02-22 杜邦营养生物科学有限公司 Use of xylanase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0463706A1 (en) * 1990-07-24 1992-01-02 Gist-Brocades N.V. Cloning and expression of xylanase genes from fungal origin
EP0506190A1 (en) * 1991-03-27 1992-09-30 Gist-Brocades N.V. Cloning and expression of genes encoding arabinan-degrading enzymes of fungal origin
WO1993011249A1 (en) * 1991-12-04 1993-06-10 Novo Nordisk A/S A method of cloning proteins in yeast and cellulase from humicola insolens

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0463706A1 (en) * 1990-07-24 1992-01-02 Gist-Brocades N.V. Cloning and expression of xylanase genes from fungal origin
EP0506190A1 (en) * 1991-03-27 1992-09-30 Gist-Brocades N.V. Cloning and expression of genes encoding arabinan-degrading enzymes of fungal origin
WO1993011249A1 (en) * 1991-12-04 1993-06-10 Novo Nordisk A/S A method of cloning proteins in yeast and cellulase from humicola insolens

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
H. HESSE ET AL.: "An improved method for generating subtracted cDNA libraries using phage lambda vectors", NUCLEIC ACIDS RESEARCH, vol. 23, no. 16, 25 August 1995 (1995-08-25), IRL PRESS LIMITED,OXFORD,ENGLAND, pages 3355 - 3356, XP002024451 *
J.M. SHORT ET AL.: "Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties", NUCLEIC ACIDS RESEARCH, vol. 16, no. 15, 1988, IRL PRESS LIMITED,OXFORD,ENGLAND, pages 7583 - 7600, XP000568110 *
K.K. STANLEY AND J.P. LUZIO: "Construction of a new bacterial expression vectors: identification of cDNA clones coding for human liver proteins", EMBO J., vol. 3, no. 6, June 1984 (1984-06-01), OXFORD UNIVERSITY PRESS,GB;, pages 1429 - 1434, XP000568110 *
KORMELINK F J M ET AL: "PURIFICATION AND CHARACTERIZATION OF A (1,4)-BETA-D-ARABINOXYLAN ARABINOFURANOHYDROLASE FROM ASPERGILLUS AWAMORI", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 35, no. 6, 1 September 1991 (1991-09-01), pages 753 - 758, XP000567078 *
P. KARLOVSKY AND G.A. WOLF: "Screening of expression cDNA libraries for polysaccharide hydrolysing enzymes: isolation of cDNA clones encoding cellulase, xylanase, and beta-1,3-glucanase of Phytophora parasitica", METHODS IN MOLECULAR AND CELLULAR BIOLOGY, vol. 4, no. 1, 1993, WILEY-LISS, INC., US, pages 40 - 45, XP000567054 *
Y. GOTO ET AL.: "A novel human insulinoma-associated cDNA, IA-1, encodes a protein with "zinc-finger" DNA-binding motifs", J. BIOL. CHEM., vol. 267, no. 21, 25 July 1992 (1992-07-25), AM. SOC. BIOCHEM. MOL.BIOL.,INC.,BALTIMORE,US, pages 15252 - 15257, XP002024452 *

Also Published As

Publication number Publication date
JPH10510720A (en) 1998-10-20
WO1997013853A2 (en) 1997-04-17
EP0796328A2 (en) 1997-09-24
AU7294396A (en) 1997-04-30

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