WO1997012961B1 - Augmentation du nombre de cellules adultes des ilots de langerhans du pancreas, a l'aide de laminine-5 - Google Patents
Augmentation du nombre de cellules adultes des ilots de langerhans du pancreas, a l'aide de laminine-5Info
- Publication number
- WO1997012961B1 WO1997012961B1 PCT/US1996/015167 US9615167W WO9712961B1 WO 1997012961 B1 WO1997012961 B1 WO 1997012961B1 US 9615167 W US9615167 W US 9615167W WO 9712961 B1 WO9712961 B1 WO 9712961B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- iccs
- matrix
- cells
- laminin
- human
- Prior art date
Links
- 108010028309 kalinin Proteins 0.000 title claims abstract 11
- 210000004153 islets of langerhan Anatomy 0.000 title claims abstract 5
- 238000000034 method Methods 0.000 claims abstract 22
- 210000004027 cell Anatomy 0.000 claims abstract 13
- 239000011159 matrix material Substances 0.000 claims abstract 11
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims abstract 10
- 102000004877 Insulin Human genes 0.000 claims abstract 5
- 108090001061 Insulin Proteins 0.000 claims abstract 5
- 229940125396 insulin Drugs 0.000 claims abstract 5
- 210000002744 extracellular matrix Anatomy 0.000 claims abstract 3
- 206010005003 Bladder cancer Diseases 0.000 claims abstract 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims abstract 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims abstract 2
- 201000001531 bladder carcinoma Diseases 0.000 claims abstract 2
- 208000010570 urinary bladder carcinoma Diseases 0.000 claims abstract 2
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 3
- 108020004511 Recombinant DNA Proteins 0.000 claims 2
- 238000002513 implantation Methods 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- HBOMLICNUCNMMY-KJFJCRTCSA-N 1-[(4s,5s)-4-azido-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1C1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-KJFJCRTCSA-N 0.000 claims 1
- YTOPFCCWCSOHFV-UHFFFAOYSA-N 2,6-dimethyl-4-tridecylmorpholine Chemical compound CCCCCCCCCCCCCN1CC(C)OC(C)C1 YTOPFCCWCSOHFV-UHFFFAOYSA-N 0.000 claims 1
- 102100022744 Laminin subunit alpha-3 Human genes 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- 230000004888 barrier function Effects 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000002775 capsule Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 210000002919 epithelial cell Anatomy 0.000 claims 1
- 230000002349 favourable effect Effects 0.000 claims 1
- 210000000301 hemidesmosome Anatomy 0.000 claims 1
- 230000002480 immunoprotective effect Effects 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 210000003734 kidney Anatomy 0.000 claims 1
- 210000004185 liver Anatomy 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
Abstract
L'invention concerne un procédé qui permet d'augmenter le nombre des cellules adultes des îlots de Langerhans du pancreas, aux fins de transplantation, et consiste à mettre en contact ces cellules avec une matrice extracellulaire de laminine 5. Après trois passages en culture, le nombre des cellules est multiplié par 1500 suite au contact avec la matrice déposée provenant de cellules d'un carcinome de vessie de rat de type 804G. On observe également une augmentation du nombre des cellules des îlots au contact d'une matrice soluble de type 804G. Les nouvelles cellules des îlots de Langerhans du pancreas ainsi obtenues contiennent de l'insuline et réagissent à l'épreuve de tolérance au glucose.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9514294A JPH11513253A (ja) | 1995-10-06 | 1996-09-23 | 成体膵島細胞のラミニン５による成長 |
| EP96935891A EP0859830B1 (fr) | 1995-10-06 | 1996-09-23 | Augmentation du nombre de cellules adultes des ilots de langerhans du pancreas, a l'aide de laminine-5 |
| AU73672/96A AU706581B2 (en) | 1995-10-06 | 1996-09-23 | Growth of adult pancreatic islet cells |
| DE69630972T DE69630972T2 (de) | 1995-10-06 | 1996-09-23 | Wachstum von adulten langenhans-zellen des pankreas mit laminin-5 |
| AT96935891T ATE255631T1 (de) | 1995-10-06 | 1996-09-23 | Wachstum von adulten langenhans-zellen des pankreas mit laminin-5 |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US487895P | 1995-10-06 | 1995-10-06 | |
| US60/004,878 | 1995-10-06 | ||
| US08/626,394 | 1996-03-29 | ||
| US08/626,394 US5681587A (en) | 1995-10-06 | 1996-03-29 | Growth of adult pancreatic islet cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO1997012961A2 WO1997012961A2 (fr) | 1997-04-10 |
| WO1997012961A3 WO1997012961A3 (fr) | 1997-05-22 |
| WO1997012961B1 true WO1997012961B1 (fr) | 1997-07-10 |
Family
ID=26673609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/015167 WO1997012961A2 (fr) | 1995-10-06 | 1996-09-23 | Augmentation du nombre de cellules adultes des ilots de langerhans du pancreas, a l'aide de laminine-5 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5681587A (fr) |
| EP (1) | EP0859830B1 (fr) |
| JP (1) | JPH11513253A (fr) |
| AT (1) | ATE255631T1 (fr) |
| AU (1) | AU706581B2 (fr) |
| CA (1) | CA2233859A1 (fr) |
| DE (1) | DE69630972T2 (fr) |
| ES (1) | ES2211988T3 (fr) |
| WO (1) | WO1997012961A2 (fr) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6231881B1 (en) | 1992-02-24 | 2001-05-15 | Anton-Lewis Usala | Medium and matrix for long-term proliferation of cells |
| US6352707B1 (en) | 1992-02-24 | 2002-03-05 | Anton-Lewis Usala | Transplant encapsulation in a hydrogel matrix to obscure immune recognition |
| USRE36844E (en) * | 1993-04-05 | 2000-08-29 | Desmos Incorporated | Cellular attachment to trans-epithelial appliances |
| US5672361A (en) * | 1996-03-29 | 1997-09-30 | Desmos, Inc. | Laminin 5 for growth of pancreatic islet cells |
| US6294356B1 (en) | 1998-01-16 | 2001-09-25 | Northwestern University | Methods and materials for making and using laminin-5 |
| AU3572299A (en) * | 1998-04-22 | 1999-11-08 | Desmos, Inc. | Creation of bioactive surfaces through selective adsorption |
| AU4544299A (en) * | 1998-05-29 | 1999-12-13 | Cythera, Inc. | Promotion of cell differentiation by initially passaged cells |
| JP2004500012A (ja) * | 1998-10-13 | 2004-01-08 | ザ・ジェネラル・ホスピタル・コーポレイション | ラミニン５、１３及び１４並びにそれらの利用 |
| US6242666B1 (en) | 1998-12-16 | 2001-06-05 | The Scripps Research Institute | Animal model for identifying a common stem/progenitor to liver cells and pancreatic cells |
| US6638765B1 (en) * | 1999-02-04 | 2003-10-28 | Mcgill University | Platform for the differentiation of cells |
| US6303355B1 (en) | 1999-03-22 | 2001-10-16 | Duke University | Method of culturing, cryopreserving and encapsulating pancreatic islet cells |
| US6365385B1 (en) | 1999-03-22 | 2002-04-02 | Duke University | Methods of culturing and encapsulating pancreatic islet cells |
| US6703363B1 (en) * | 1999-04-30 | 2004-03-09 | Biostratum, Inc. | Recombinant laminin 5 |
| EP1177290A2 (fr) * | 1999-04-30 | 2002-02-06 | Biostratum, Inc. | Laminine 5 recombinee |
| US6638907B1 (en) | 1999-04-30 | 2003-10-28 | Biostratum, Inc. | Laminin 8 and methods for its use |
| AU4496800A (en) | 1999-04-30 | 2000-11-17 | University Of Medicine And Dentistry Of New Jersey | Laminin 2 and methods for its use |
| US6815203B1 (en) | 1999-06-23 | 2004-11-09 | Joslin Diabetes Center, Inc. | Methods of making pancreatic islet cells |
| CA2375509A1 (fr) * | 1999-06-23 | 2000-12-28 | Joslin Diabetes Center, Inc | Fabrication d'ilots de cellules pancreatiques |
| AU7719300A (en) * | 1999-09-27 | 2001-04-30 | Ixion Biotechnology, Inc. | Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures |
| AU2001233502B2 (en) * | 2000-02-18 | 2006-02-02 | The Walter And Eliza Hall Institute Of Medical Research | Pancreatic islet cell growth factors |
| JP2008520235A (ja) * | 2004-11-22 | 2008-06-19 | ラモト アット テル アヴィヴ ユニヴァーシティ リミテッド | インスリンを産生可能な拡大され、かつ再分化した成体島β細胞の集団およびその作製方法 |
| US8735154B2 (en) * | 2006-10-30 | 2014-05-27 | The University Of Kansas | Templated islet cells and small islet cell clusters for diabetes treatment |
| US20080103606A1 (en) * | 2006-10-30 | 2008-05-01 | Cory Berkland | Templated islet cells and small islet cell clusters for diabetes treatment |
| US8895048B2 (en) * | 2010-04-06 | 2014-11-25 | The University Of Kansas | Templated islet cells and small islet cell clusters for diabetes treatment |
| AU2011247984B2 (en) * | 2010-05-07 | 2015-04-30 | University Of North Carolina At Chapel Hill | Method of engrafting cells from solid tissues |
| KR101327630B1 (ko) * | 2012-03-05 | 2013-11-13 | 울산대학교 산학협력단 | 아텔로콜라겐을 이용한 췌도세포 이식용 담체의 제조방법 그리고 이를 이용하여 제조된 인공췌장 |
| WO2019098944A1 (fr) * | 2017-11-15 | 2019-05-23 | National University Of Singapore | Systèmes de développement d'îlots pancréatiques et leur transplantation |
| CN113308433A (zh) * | 2021-04-27 | 2021-08-27 | 立沃生物科技（深圳）有限公司 | 一种胰岛体外培养表面杂细胞处理方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2106955C (fr) * | 1991-03-26 | 2003-05-06 | Robert E. Burgeson | Produit et methode d'amelioration de l'adhesion des keratinocytes au derme |
| JPH08502733A (ja) * | 1992-08-28 | 1996-03-26 | オレゴン州 | 真皮に対する角化細胞の接着を改善する生成物および方法 |
| US5422264A (en) * | 1993-11-12 | 1995-06-06 | Desmos, Inc. | Soluble factor stimulation of attachment and hemidesmosome assembly in epithelial cells |
| US5510263A (en) * | 1993-04-05 | 1996-04-23 | Desmos, Inc. | Growth of pancreatic islet-like cell clusters |
| US5541106A (en) * | 1993-04-05 | 1996-07-30 | Desmos, Inc. | Cell matrix stimulated attachment and hemidesmosome assembly |
| AU7872894A (en) * | 1993-09-02 | 1995-03-22 | Fred Hutchinson Cancer Research Center | Epiligrin, an epithelial ligand for integrins |
| US5834308A (en) * | 1994-04-28 | 1998-11-10 | University Of Florida Research Foundation, Inc. | In vitro growth of functional islets of Langerhans |
-
1996
- 1996-03-29 US US08/626,394 patent/US5681587A/en not_active Expired - Fee Related
- 1996-09-23 AU AU73672/96A patent/AU706581B2/en not_active Ceased
- 1996-09-23 JP JP9514294A patent/JPH11513253A/ja not_active Ceased
- 1996-09-23 WO PCT/US1996/015167 patent/WO1997012961A2/fr active IP Right Grant
- 1996-09-23 DE DE69630972T patent/DE69630972T2/de not_active Expired - Fee Related
- 1996-09-23 ES ES96935891T patent/ES2211988T3/es not_active Expired - Lifetime
- 1996-09-23 CA CA002233859A patent/CA2233859A1/fr not_active Abandoned
- 1996-09-23 EP EP96935891A patent/EP0859830B1/fr not_active Expired - Lifetime
- 1996-09-23 AT AT96935891T patent/ATE255631T1/de not_active IP Right Cessation
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