+

WO1997007220A3 - Mutagenese par insertion de lieurs au moyen du systeme de transposition de la sequence d'insertion is21 - Google Patents

Mutagenese par insertion de lieurs au moyen du systeme de transposition de la sequence d'insertion is21 Download PDF

Info

Publication number
WO1997007220A3
WO1997007220A3 PCT/EP1996/003625 EP9603625W WO9707220A3 WO 1997007220 A3 WO1997007220 A3 WO 1997007220A3 EP 9603625 W EP9603625 W EP 9603625W WO 9707220 A3 WO9707220 A3 WO 9707220A3
Authority
WO
WIPO (PCT)
Prior art keywords
linker
mutagenesis
transposition system
proteins
sequence
Prior art date
Application number
PCT/EP1996/003625
Other languages
German (de)
English (en)
Other versions
WO1997007220A2 (fr
Inventor
Dieter Haas
Thomas Seitz
Bernard Berger
Sergio Schmid
Original Assignee
Boehringer Mannheim Gmbh
Dieter Haas
Thomas Seitz
Bernard Berger
Sergio Schmid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh, Dieter Haas, Thomas Seitz, Bernard Berger, Sergio Schmid filed Critical Boehringer Mannheim Gmbh
Publication of WO1997007220A2 publication Critical patent/WO1997007220A2/fr
Publication of WO1997007220A3 publication Critical patent/WO1997007220A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1018—Carboxy- and carbamoyl transferases (2.1.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Le procédé de génie génétique décrit permet d'insérer de 4 à 11 codons dans n'importe quel site de gènes clonés. Dans des gènes qui codent des protéines le schéma de lecture n'est pas modifié par une insertion. Les insertions modifient la structure des protéines, ce qui peut conférer aux protéines une activité biologique modifiée. La transposition réalisée selon ce procédé peut se faire in vivo, par exemple dans Escherichia coli, ou in vitro. Le procédé peut également être appliqué pour l'insertion d'épitopes.
PCT/EP1996/003625 1995-08-18 1996-08-16 Mutagenese par insertion de lieurs au moyen du systeme de transposition de la sequence d'insertion is21 WO1997007220A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH2373/95-3 1995-08-18
CH237395 1995-08-18

Publications (2)

Publication Number Publication Date
WO1997007220A2 WO1997007220A2 (fr) 1997-02-27
WO1997007220A3 true WO1997007220A3 (fr) 1997-03-20

Family

ID=4232259

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1996/003625 WO1997007220A2 (fr) 1995-08-18 1996-08-16 Mutagenese par insertion de lieurs au moyen du systeme de transposition de la sequence d'insertion is21

Country Status (1)

Country Link
WO (1) WO1997007220A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2829986B1 (fr) 2001-09-26 2003-12-26 Roulements Soc Nouvelle Systeme de direction assistee electrique commande par impulsions

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BACTERIAL CONJUGATION (CLEWELL, D., ed.), PLENUM PRESS, New York, 1993; Chapter 6; REIMMANN, C. & HAAS, D.:'Mobilization of chromosomes and nonconjugative plasmids bycointegrative mechanisms.' *
Diss. ETH Nr. 10197. ZÜRICH 1993. SCHMID, S.:'Die Insertionssequenz IS21: Entwicklung eines In-vitro Integrationssystems' *
HOEKSTRA, M. ET AL.: "A Tn3 derivative that can be used to make short in-frame insertions within genes", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 88, June 1991 (1991-06-01), WASHINGTON US, pages 5457 - 5461, XP002024153 *

Also Published As

Publication number Publication date
WO1997007220A2 (fr) 1997-02-27

Similar Documents

Publication Publication Date Title
NZ243500A (en) Nucleic acid encoding dihydrokaempferol hydroxylating enzyme (dhk) and its use in producing transgenic flowering plants
AU568067B2 (en) Oligonucleotide therapeutic agent and methods of making same
AU7550098A (en) System for expressing hyperthermostable protein
ES8705521A1 (es) Un metodo de producir un polipetido similar a factor de necrosis de tumores (tnf) de mamiferos.
NZ335945A (en) Presenilin proteins and genetic sequences that are related to Alzheimer's disease
DE69839401D1 (en) Dcr3 polypeptid, ein tnfr homolog
CA2200371A1 (fr) Gene humain npik
NZ504214A (en) Recombination cloning using nucleic acids having recombination sites
SI0917571T1 (en) Agouti-related gene
IL173399A0 (en) Circular dna molecule with conditional origin of replication which contains nucleic acid of interest and selection marker but does not encode its replication initiation protein, method for preparing the same and use thereof in gene therapy
GR1005153B (el) Πρωτεινες συντηξεως, παρασκευη τους και χρηση τους.
AU5233686A (en) Dna sequence encoding a hirudin-like protein and process for preparing such protein
WO1998028317A3 (fr) Synthese chimique d'analogues de nucleosides et leur incorporation dans des polynucleotides
GR852940B (fr)
WO1994005791A3 (fr) Facteur influant sur la croissance du tissu dorsal et compositions
MX9708426A (es) Polipeptido hk2 de variante estable.
AU8748198A (en) Carcinostatic agents
EP0967283A4 (fr) Genes codant pour des proteines a activite de transglycolsylation
MX9806041A (es) Proteina purificada sr-p70.
NO971139D0 (no) Fremgangsmåte for fremstilling av rekombinante proteiner, plasmider og modifiserte celler
WO1997007220A3 (fr) Mutagenese par insertion de lieurs au moyen du systeme de transposition de la sequence d'insertion is21
EP0816504A3 (fr) Facteur d'activation des plaquettes acétylhydrolase, et gène codant celui-ci
AU7949991A (en) Cloned n-methylhydantoinase
WO1998027200A3 (fr) Sequences consensus de l'origine de replication d'adn humain et d'adn de mammifere
WO1998003653A3 (fr) Procede de preparation d'organes de mammiferes non humains transgeniques en vue de leur transplantation chez l'homme, et sequences nucleotidiques pour la mise en oeuvre de ce procede

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE

AK Designated states

Kind code of ref document: A3

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载