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WO1997048120A1 - Spectrometre de masse a temps de vol - Google Patents

Spectrometre de masse a temps de vol Download PDF

Info

Publication number
WO1997048120A1
WO1997048120A1 PCT/GB1997/001552 GB9701552W WO9748120A1 WO 1997048120 A1 WO1997048120 A1 WO 1997048120A1 GB 9701552 W GB9701552 W GB 9701552W WO 9748120 A1 WO9748120 A1 WO 9748120A1
Authority
WO
WIPO (PCT)
Prior art keywords
ions
velocity
fragmentation
mass spectrometer
interest
Prior art date
Application number
PCT/GB1997/001552
Other languages
English (en)
Inventor
Alexander Alekseevich Makarov
Stephen Charles Davis
Original Assignee
Hd Technologies Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hd Technologies Limited filed Critical Hd Technologies Limited
Priority to AU30411/97A priority Critical patent/AU3041197A/en
Publication of WO1997048120A1 publication Critical patent/WO1997048120A1/fr

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • H01J49/34—Dynamic spectrometers
    • H01J49/40—Time-of-flight spectrometers
    • H01J49/401—Time-of-flight spectrometers characterised by orthogonal acceleration, e.g. focusing or selecting the ions, pusher electrode
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn

Definitions

  • This invention relates to improvements in or relating to mass
  • a tandem mass spectrometer usually
  • spectrometer which can operate both as a single spectrometer and also as
  • spectrometer of the time-of-flight kind comprising an ion source to produce
  • ions for analysis said ions having a velocity in a first direction, means to
  • Fig. 1 shows a diagrammatic representation of
  • time-of-flight mass spectrometer in
  • Fig. 2 shows the spectrometer of Fig. 1 with the
  • Fig. 3 shows a diagrammatic sectional side view
  • Fig. 4 shows a schematic perspective view of an
  • Fig. 1 there is shown a mass spectrometer of time-
  • the spectrometer comprises an ion source 10 which produces a beam
  • an ion optical arrangement 20 which acts to focus and accelerate
  • an extraction device 30 which acts to orthogonally extract from the beam of ions produced by the ion
  • fragmentation device 60 which acts to fragment selected ions of interest
  • the ion source 10 is of conventional form and may form ions utilising
  • the ion source may use electron impact, chemical ionization, thermo or
  • the ion source can generate either a continuous beam
  • the ion optical arrangement 20 contains any suitable arrangement
  • the extraction device 30 comprises electrodes 31 and 32 which are connected to each other.
  • At least electrode 32 which is positioned adjacent to the measurement chamber 50 is either in the form
  • the measurement chamber 50 is generally of conventional form and
  • a perforated grid electrode 34 is provided with a perforated grid electrode 34, or an electrode having slits
  • a detector 70 of conventional form is located
  • the measurement chamber is of importance for a reason which will become
  • the fragmentation device 60 acts to fragment selected ions of
  • the fragmentation device 60 is located
  • the fragmentation device 60 can operate to fragment the ions in any
  • One form of fragmentation device 60 is shown more clearly in Fig. 3.
  • This fragmentation device comprises a fragmentation region defined
  • an end cap 62 which may have a transparent or at least
  • a gas leak valve 64 may also be provided linked to the
  • ion guide 66 extends generally along a horizontal axis of the device for a
  • optics static or dynamic may be used within the fragmentation device 60.
  • an ion beam is produced by an ion source 10 which exits the
  • the ion beam passes through the ion optical arrangement 20 in which
  • the beam is focused into an ion beam of low divergence.
  • the electrode 31 is applied to the electrode 31 using a pulsed voltage supply 33.
  • electrode 32 is maintained at a fixed potential whilst electrode 31 is pulsed to a voltage V greater than the fixed potential at which electrode 32 is
  • electrode 32 can be pulsed to a voltage lower
  • Electrode 32 is arranged to be formed either from a perforated grid or to
  • the beam can be further processed
  • the measurement chamber 50 of the spectrometer can be achieved.
  • the ions within the section of the beam will not only have a velocity in
  • X-velocity component of velocity in the direction X
  • the detector may be
  • the ion mirror 40 acts to reflect ions which interact with it so as to cause them to travel in the
  • An ion mirror 40 of this type is usually referred to as a
  • section of ion beam is directed so as to impinge upon the detector 70
  • the ion mirror 40 the beam section is directed so as to pass the detector 70 along a second path P 2 shown in Fig. 1 and to exit the measurement
  • mass/charge ratio can be selected for fragmentation.
  • fragmentation device the ion of interest is fragmented into ions of smaller
  • E is the mean initial acceleration voltage in the ion source 10 and ion
  • ⁇ v x is the spread of velocities in direction X resulting
  • v ⁇ is the mean velocity in the
  • R is the mass resolution as a single mass spectrometer
  • V is a voltage
  • L M is effective path
  • V a is the mean voltage
  • fragmented ions have been formed, they are ejected in a manner to be
  • the ions of interest are fragmented into smaller ions using either multiple
  • CID gas molecules
  • an intense light beam can be directed at the ions of interest
  • a leak valve 64 introduced into the fragmentation region through a leak valve 64 and may
  • fragmentation region is detected using ionization gauge 65.
  • the beam of fragmented ions will exit the fragmentation device 60 and may
  • the measurement chamber 50 the measurement chamber 50.
  • fragment ions have a similar velocity to the ion of interest from which they
  • a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a voltage can be achieved in a number of different ways. Firstly, if desired, a
  • the lighter fragments are
  • Any type of ion optical arrangement 36 either transmitting
  • the fragmenting means 60 by an additional radio-frequency or impulsive
  • deflector plates 51 and 52 are provided in the measurement chamber.
  • the pairs of deflector plates 51 , 52 act to deflect an ion beam passing through the measurement chamber 50.
  • the second pair of deflector plates 52 deflects the ion beam by
  • the extraction device may of course be constructed in several sections. In these
  • a further electrode 37 is provided between the ion source
  • optical arrangement 20 could be used to reflect the beam.
  • more than one detector 70 may be provided

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

L'invention concerne un spectromètre de masse à temps de vol. Ce spectromètre comprend une source d'ions qui produit des ions en vue de l'analyse. Lors de leur production à partir de la source, ces ions présentent une vitesse dans une première direction. Les ions passent entre deux électrodes. Sur l'une de ces électrodes, une tension est appliquée qui impose sur les ions une vitesse dans une deuxième direction pour les transporter dans une chambre de mesure renfermant un détecteur. Les ions présentant un intérêt spécifique, d'un rapport m/z particulier, sont sélectionnés, fragmentés dans un dispositif de fragmentation et détectés par le détecteur qui produit des spectres de masse en fonction des ions de masse inférieure détectés. Les ions présentant un intérêt particulier sont sélectionnés lors de leur passage entre deux électrodes. Sur l'une de ces électrodes, un champ électrique est appliqué qui n'affecte que les ions présentant un intérêt spécifique en réduisant sensiblement à zéro la vitesse dans la deuxième direction, au voisinage du dispositif de fragmentation. La vitesse dans la première direction ne provoque l'entrée dans le dispositif de fragmentation en vue de la fragmentation que des ions présentant un intérêt spécifique.
PCT/GB1997/001552 1996-06-10 1997-06-10 Spectrometre de masse a temps de vol WO1997048120A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU30411/97A AU3041197A (en) 1996-06-10 1997-06-10 Time-of-flight mass spectrometer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9612091.0A GB9612091D0 (en) 1996-06-10 1996-06-10 Improvements in or relating to time-of-flight mass spectrometers
GB9612091.0 1996-06-10

Publications (1)

Publication Number Publication Date
WO1997048120A1 true WO1997048120A1 (fr) 1997-12-18

Family

ID=10795038

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1997/001552 WO1997048120A1 (fr) 1996-06-10 1997-06-10 Spectrometre de masse a temps de vol

Country Status (3)

Country Link
AU (1) AU3041197A (fr)
GB (1) GB9612091D0 (fr)
WO (1) WO1997048120A1 (fr)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999040610A3 (fr) * 1998-02-06 1999-10-07 Perseptive Biosystems Inc Spectrometre de masse a temps de vol en tandem avec extraction differee et procede d'utilisation
RU2143110C1 (ru) * 1998-12-25 1999-12-20 Научно-исследовательский институт ядерной физики при Томском политехническом университете Масс-спектрометр ишкова
WO2001011660A1 (fr) * 1999-08-10 2001-02-15 Gbc Scientific Equipment Pty Ltd Spectrometre de masse a temps de vol comportant un accelerateur orthogonal
US6717132B2 (en) 2000-02-09 2004-04-06 Bruker Daltonik Gmbh Gridless time-of-flight mass spectrometer for orthogonal ion injection
US6903332B2 (en) * 2001-11-30 2005-06-07 Bruker Daltonik Gmbh Pulsers for time-of-flight mass spectrometers with orthogonal ion injection
GB2419032A (en) * 2003-11-17 2006-04-12 Micromass Ltd A mass filter for a mass spectrometer
WO2006103412A2 (fr) 2005-03-29 2006-10-05 Thermo Finnigan Llc Ameliorations concernant la spectrometrie de masse
US7297960B2 (en) 2003-11-17 2007-11-20 Micromass Uk Limited Mass spectrometer
GB2454767A (en) * 2007-10-10 2009-05-20 Bruker Daltonik Gmbh Producing cleaned daughter ion spectra from a MALDI ionisation TOF mass spectrometer
DE102004060888B4 (de) * 2003-12-22 2009-11-19 Micromass Uk Ltd. Verfahren zur Reduzierung der Effekte eines chemischen Hintergrundrauschens in Massenspektren
US7759638B2 (en) 2005-03-29 2010-07-20 Thermo Finnigan Llc Mass spectrometer
GB2485825A (en) * 2010-11-26 2012-05-30 Thermo Fisher Scient Bremen Method of mass selecting ions and mass selector therefor
US8742333B2 (en) 2010-09-17 2014-06-03 Wisconsin Alumni Research Foundation Method to perform beam-type collision-activated dissociation in the pre-existing ion injection pathway of a mass spectrometer
US8909481B2 (en) 2000-12-26 2014-12-09 The Institute Of Systems Biology Method of mass spectrometry for identifying polypeptides
US9196466B2 (en) 2002-07-24 2015-11-24 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4851669A (en) * 1988-06-02 1989-07-25 The Regents Of The University Of California Surface-induced dissociation for mass spectrometry
US5202563A (en) * 1991-05-16 1993-04-13 The Johns Hopkins University Tandem time-of-flight mass spectrometer
GB2274197A (en) * 1993-01-11 1994-07-13 Kratos Analytical Ltd Time-of-flight mass spectrometer

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4851669A (en) * 1988-06-02 1989-07-25 The Regents Of The University Of California Surface-induced dissociation for mass spectrometry
US5202563A (en) * 1991-05-16 1993-04-13 The Johns Hopkins University Tandem time-of-flight mass spectrometer
GB2274197A (en) * 1993-01-11 1994-07-13 Kratos Analytical Ltd Time-of-flight mass spectrometer

Cited By (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348688B1 (en) 1998-02-06 2002-02-19 Perseptive Biosystems Tandem time-of-flight mass spectrometer with delayed extraction and method for use
US6770870B2 (en) 1998-02-06 2004-08-03 Perseptive Biosystems, Inc. Tandem time-of-flight mass spectrometer with delayed extraction and method for use
WO1999040610A3 (fr) * 1998-02-06 1999-10-07 Perseptive Biosystems Inc Spectrometre de masse a temps de vol en tandem avec extraction differee et procede d'utilisation
RU2143110C1 (ru) * 1998-12-25 1999-12-20 Научно-исследовательский институт ядерной физики при Томском политехническом университете Масс-спектрометр ишкова
WO2001011660A1 (fr) * 1999-08-10 2001-02-15 Gbc Scientific Equipment Pty Ltd Spectrometre de masse a temps de vol comportant un accelerateur orthogonal
US6717132B2 (en) 2000-02-09 2004-04-06 Bruker Daltonik Gmbh Gridless time-of-flight mass spectrometer for orthogonal ion injection
DE10005698B4 (de) * 2000-02-09 2007-03-01 Bruker Daltonik Gmbh Gitterloses Reflektor-Flugzeitmassenspektrometer für orthogonalen Ioneneinschuss
US8909481B2 (en) 2000-12-26 2014-12-09 The Institute Of Systems Biology Method of mass spectrometry for identifying polypeptides
US6903332B2 (en) * 2001-11-30 2005-06-07 Bruker Daltonik Gmbh Pulsers for time-of-flight mass spectrometers with orthogonal ion injection
US9384951B2 (en) 2002-07-24 2016-07-05 Micromass Uk Limited Mass analysis using alternating fragmentation modes
US10083825B2 (en) 2002-07-24 2018-09-25 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device
US9196466B2 (en) 2002-07-24 2015-11-24 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device
US9697995B2 (en) 2002-07-24 2017-07-04 Micromass Uk Limited Mass spectrometer with bypass of a fragmentation device
GB2408144B (en) * 2003-11-17 2006-04-26 Micromass Ltd Mass spectrometer
US7297960B2 (en) 2003-11-17 2007-11-20 Micromass Uk Limited Mass spectrometer
GB2419032B (en) * 2003-11-17 2006-07-26 Micromass Ltd Mass spectrometer
DE102004055279B4 (de) * 2003-11-17 2010-12-02 Micromass Uk Ltd. Massenspektrometer
GB2419032A (en) * 2003-11-17 2006-04-12 Micromass Ltd A mass filter for a mass spectrometer
DE102004060888B4 (de) * 2003-12-22 2009-11-19 Micromass Uk Ltd. Verfahren zur Reduzierung der Effekte eines chemischen Hintergrundrauschens in Massenspektren
US7759638B2 (en) 2005-03-29 2010-07-20 Thermo Finnigan Llc Mass spectrometer
WO2006103412A2 (fr) 2005-03-29 2006-10-05 Thermo Finnigan Llc Ameliorations concernant la spectrometrie de masse
GB2454767B (en) * 2007-10-10 2012-05-23 Bruker Daltonik Gmbh Method and apparatus for producing cleaned daughter ion spectra from MALDI ionization
US7989759B2 (en) 2007-10-10 2011-08-02 Bruker Daltonik Gmbh Cleaned daughter ion spectra from maldi ionization
GB2454767A (en) * 2007-10-10 2009-05-20 Bruker Daltonik Gmbh Producing cleaned daughter ion spectra from a MALDI ionisation TOF mass spectrometer
US8742333B2 (en) 2010-09-17 2014-06-03 Wisconsin Alumni Research Foundation Method to perform beam-type collision-activated dissociation in the pre-existing ion injection pathway of a mass spectrometer
US9053916B2 (en) 2010-09-17 2015-06-09 Wisconsin Alumni Research Foundation Method to perform beam-type collision-activated dissociation in the pre-existing ion injection pathway of a mass spectrometer
US9478405B2 (en) 2010-09-17 2016-10-25 Wisconsin Alumni Research Foundation Method to perform beam-type collision-activated dissociation in the pre-existing ion injection pathway of a mass spectrometer
GB2485825A (en) * 2010-11-26 2012-05-30 Thermo Fisher Scient Bremen Method of mass selecting ions and mass selector therefor
WO2012069596A1 (fr) 2010-11-26 2012-05-31 Thermo Fisher Scientific (Bremen) Gmbh Procédé de sélection de masse d'ions et sélecteur de masse
GB2485825B (en) * 2010-11-26 2015-05-20 Thermo Fisher Scient Bremen Method of mass selecting ions and mass selector
DE112011103930B4 (de) 2010-11-26 2017-11-09 Thermo Fisher Scientific (Bremen) Gmbh Verfahren zum Massenselektieren von Ionen und Massenselektor

Also Published As

Publication number Publication date
GB9612091D0 (en) 1996-08-14
AU3041197A (en) 1998-01-07

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