WO1996039101B1 - Compositions and methods for naturally secreted extracellular matrix - Google Patents
Compositions and methods for naturally secreted extracellular matrixInfo
- Publication number
- WO1996039101B1 WO1996039101B1 PCT/US1996/009482 US9609482W WO9639101B1 WO 1996039101 B1 WO1996039101 B1 WO 1996039101B1 US 9609482 W US9609482 W US 9609482W WO 9639101 B1 WO9639101 B1 WO 9639101B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- extracellular matrix
- cells
- naturally secreted
- framework
- composition according
- Prior art date
Links
- 210000002744 extracellular matrix Anatomy 0.000 title claims abstract 18
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 title claims abstract 17
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 title claims abstract 17
- 238000000034 method Methods 0.000 title claims abstract 12
- 239000000203 mixture Substances 0.000 title claims abstract 11
- 210000004027 cell Anatomy 0.000 claims abstract 9
- 210000001519 tissue Anatomy 0.000 claims abstract 8
- 230000007547 defect Effects 0.000 claims abstract 5
- -1 polypropylene Polymers 0.000 claims 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims 2
- 229920001661 Chitosan Polymers 0.000 claims 2
- 229920000742 Cotton Polymers 0.000 claims 2
- 229920002307 Dextran Polymers 0.000 claims 2
- 108010010803 Gelatin Proteins 0.000 claims 2
- 239000000020 Nitrocellulose Substances 0.000 claims 2
- 239000004952 Polyamide Substances 0.000 claims 2
- 239000004743 Polypropylene Substances 0.000 claims 2
- 239000004793 Polystyrene Substances 0.000 claims 2
- 239000002729 catgut Substances 0.000 claims 2
- 229920002678 cellulose Polymers 0.000 claims 2
- 239000001913 cellulose Substances 0.000 claims 2
- 210000001612 chondrocyte Anatomy 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 2
- 239000003937 drug carrier Substances 0.000 claims 2
- 210000002889 endothelial cell Anatomy 0.000 claims 2
- 210000002919 epithelial cell Anatomy 0.000 claims 2
- 210000002950 fibroblast Anatomy 0.000 claims 2
- 229920000159 gelatin Polymers 0.000 claims 2
- 239000008273 gelatin Substances 0.000 claims 2
- 235000019322 gelatine Nutrition 0.000 claims 2
- 235000011852 gelatine desserts Nutrition 0.000 claims 2
- 229920002674 hyaluronan Polymers 0.000 claims 2
- 229960003160 hyaluronic acid Drugs 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 229920001220 nitrocellulos Polymers 0.000 claims 2
- 210000004416 odontoblast Anatomy 0.000 claims 2
- 229920000058 polyacrylate Polymers 0.000 claims 2
- 229920002647 polyamide Polymers 0.000 claims 2
- 229920000515 polycarbonate Polymers 0.000 claims 2
- 239000004417 polycarbonate Substances 0.000 claims 2
- 229920000728 polyester Polymers 0.000 claims 2
- 229920001155 polypropylene Polymers 0.000 claims 2
- 229920002223 polystyrene Polymers 0.000 claims 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims 2
- 239000011148 porous material Substances 0.000 claims 2
- 230000002207 retinal effect Effects 0.000 claims 2
- 229920002554 vinyl polymer Polymers 0.000 claims 2
- 102000008186 Collagen Human genes 0.000 claims 1
- 108010035532 Collagen Proteins 0.000 claims 1
- 102000003839 Human Proteins Human genes 0.000 claims 1
- 108090000144 Human Proteins Proteins 0.000 claims 1
- 229920000954 Polyglycolide Polymers 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000010261 cell growth Effects 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 229920001436 collagen Polymers 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000002349 favourable effect Effects 0.000 claims 1
- 210000000963 osteoblast Anatomy 0.000 claims 1
- 239000004633 polyglycolic acid Substances 0.000 claims 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 claims 1
- 239000003356 suture material Substances 0.000 claims 1
Abstract
The present invention discloses compositions containing naturally secreted human extracellular matricies, the methods of making those matricies, and the method of using those matricies. The extracellular matrix is obtained by growing cells into a three-dimensional support structure so that the cells secrete an extracellular matrix. Next, the cells are killed and removed from the support structure leaving only the extracellular matrix on the support structure. The extracellular matrix can then be removed from the support, combined with a suitable pharmacologically acceptable carrier and injected into a tissue defect area in the skin of a patient.
Claims
1. A method for the production of a naturally secreted extracellular matrix coated three-dimensional framework comprising the steps of:
(a) culturing cells on a three-dimensional framework under conditions favorable for cellular growth for a pre-determined time period such that an extracellular matrix is secreted onto the inoculated framework creating a coated framework; (b) killing the cells and
(c) removing the cells and cellular debris.
2. A method according to claim 1 wherein the three-dimensional framework is a material selected from the group consisting of polyamide, polyester, polystyrene, polypropylene, polyacrylates, polyvinyl compounds,
polycarbonate, polytetrafluorethylene, thermanox,
nitrocellulose, cotton, polyglycolie acid, catgut suture material, cellulose, gelatin, chitosan, hyaluronic acid and dextran,
3. A method according to claim 1 or 2, wherein the three-dimensional framework has pore spaces of about 150μm to about 220μm.
4. The method according to any one of claims 1 to 3, wherein the extracellular matrix is secreted by tissue specific cells selected from the group consisting of
fibroblasts, osteoblasts, odontoblasts, chondrocytes, epithelial cells, smoothst-muscle cells, retinal cells, endothelial cells, stromal cells or combinations thereof.
5. A method for the production of a naturally secreted extracellular matrix comprising carrying out steps (a), (b) and (c) according to any one of claims 1 to 4 and then (d) collecting the extracellular matrix deposited on the coated framework.
6. A composition comprising a three-dimensional framework coated with a naturally secreted extracellular matrix composed of human proteins.
7. A composition according to claim 6, wherein the framework is made from a material selected from the group consisting of polyamide, polyester, polystyrene,
polypropylene, polyacrylates, polyvinyl compounds,
polycarbonate, polytetrafluorethylene, thermanox,
nitrocellulose, cotton, polyglycolic acid, catgut sutures, cellulose, gelatin, chitosan, hyaluronic acid and dextran.
8. A composition according to claim 6 or 7, wherein the three-dimensional framework has pore spaces of about 150μm to about 220μm.
9. A composition according to any one of claims 6 to 8, wherein the naturally secreted extracellular matrix is secreted by tissue specific ceils selected from the group consisting of fibroblasts, oεteoblasts, odontoblasts, chondrocytes, epithelial cells, smooth muscle cells, retinal cells, endothelial cells, stromal cells or combinations thereof.
10. An injectable naturally secreted extracellular matrix composition for the treatment of tissue defects comprising a human naturally secreted extracellular matrix and a pharmaceutically acceptable carrier.
11. A composition according to claim 10, wherein the naturally secreted extracellular matrix is produced by a method according to claim 5.
12. A composition according to claim 11 wherein naturally secreted extracellular matrices secreted by
different tissue or cell types are mixed between said step (d) and step (e), consisting in processing the collected extracellular matrix with a pharmaceutically acceptable carrier, such that ratios of collagen types I-V, respective to each other, are adjusted.
13. A method for the repair of tissue defects comprising injecting at a site of a tissue defect a
composition according to any one of claims 10-12.
14. A method according to claim 13 wherein the naturally secreted extracellular matrix is produced by a method according to claim 5.
15. The use of a composition according to any one of claims 10-12 for the repair of tissue defects.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96921369A EP0967934B1 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods for naturally secreted extracellular matrix |
| DE69634454T DE69634454T2 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods relating to a naturally secreted matrix |
| AT96921369T ATE290350T1 (en) | 1995-06-06 | 1996-06-06 | COMPOSITIONS AND METHODS RELATING TO A NATURALLY SECRETED MATRIX |
| JP50179097A JP3709487B2 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods for naturally secreted extracellular matrix |
| AU62609/96A AU708090B2 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods for naturally secreted extracellular matrix |
| NZ311313A NZ311313A (en) | 1995-06-06 | 1996-06-06 | Composition and method for making compositions for treatment of wrinkles and scars using human extracellular matrix |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US470,101 | 1995-06-06 | ||
| US08/470,101 US5830708A (en) | 1995-06-06 | 1995-06-06 | Methods for production of a naturally secreted extracellular matrix |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1996039101A1 WO1996039101A1 (en) | 1996-12-12 |
| WO1996039101B1 true WO1996039101B1 (en) | 1997-01-09 |
Family
ID=23866272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/009482 WO1996039101A1 (en) | 1995-06-06 | 1996-06-06 | Compositions and methods for naturally secreted extracellular matrix |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5830708A (en) |
| EP (1) | EP0967934B1 (en) |
| JP (1) | JP3709487B2 (en) |
| KR (1) | KR19990022216A (en) |
| AT (1) | ATE290350T1 (en) |
| AU (1) | AU708090B2 (en) |
| CA (1) | CA2223892A1 (en) |
| DE (1) | DE69634454T2 (en) |
| ES (1) | ES2238076T3 (en) |
| NZ (1) | NZ311313A (en) |
| WO (1) | WO1996039101A1 (en) |
Families Citing this family (117)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6974571B2 (en) * | 1995-03-28 | 2005-12-13 | Thomas Jefferson University | Isolated stromal cells and methods of using the same |
| US6284284B1 (en) | 1995-06-06 | 2001-09-04 | Advanced Tissue Sciences, Inc. | Compositions and methods for production and use of an injectable naturally secreted extracellular matrix |
| US7767452B2 (en) | 1997-02-20 | 2010-08-03 | Kleinsek Don A | Tissue treatments with adipocyte cells |
| WO1998036704A1 (en) * | 1997-02-20 | 1998-08-27 | Keller Gregory S | Augmentation and repair of dermal, subcutaneous, and vocal cord tissue defects |
| GB9720987D0 (en) * | 1997-10-01 | 1997-12-03 | Biocure Ltd | A multicellular in vitro assay of angiogenesis |
| US6225118B1 (en) | 1997-10-01 | 2001-05-01 | Biocure Limited | Multicellular in vitro assay of angiogenesis |
| US6660301B1 (en) | 1998-03-06 | 2003-12-09 | Biosphere Medical, Inc. | Injectable microspheres for dermal augmentation and tissue bulking |
| WO1999047186A1 (en) * | 1998-03-18 | 1999-09-23 | University Of Pittsburgh | Chitosan-based composite materials containing glycosaminoglycan for cartilage repair |
| US6378527B1 (en) * | 1998-04-08 | 2002-04-30 | Chondros, Inc. | Cell-culture and polymer constructs |
| US6428978B1 (en) * | 1998-05-08 | 2002-08-06 | Cohesion Technologies, Inc. | Methods for the production of gelatin and full-length triple helical collagen in recombinant cells |
| PT1612265E (en) * | 1998-11-19 | 2013-03-14 | Organogenesis Inc | Bioengineered tissue constructs and methods for producing and using them |
| US7615373B2 (en) | 1999-02-25 | 2009-11-10 | Virginia Commonwealth University Intellectual Property Foundation | Electroprocessed collagen and tissue engineering |
| US6662805B2 (en) | 1999-03-24 | 2003-12-16 | The Johns Hopkins University | Method for composite cell-based implants |
| US20030007954A1 (en) * | 1999-04-12 | 2003-01-09 | Gail K. Naughton | Methods for using a three-dimensional stromal tissue to promote angiogenesis |
| US6372494B1 (en) * | 1999-05-14 | 2002-04-16 | Advanced Tissue Sciences, Inc. | Methods of making conditioned cell culture medium compositions |
| AU6072200A (en) * | 1999-07-09 | 2001-01-30 | Advanced Tissue Sciences, Inc. | Human naturally secreted extracellular matrix-coated device |
| WO2001014527A1 (en) * | 1999-08-23 | 2001-03-01 | Organogenesis Inc. | Skin care compositions and treatments |
| US20090074729A2 (en) * | 1999-11-05 | 2009-03-19 | Donald Kleinsek | Augmentation and repair of spincter defects with cells including fibroblasts |
| US7799325B2 (en) * | 1999-11-05 | 2010-09-21 | Kleinsek Donald A | Removal of hypertrophic scars |
| US20080267923A2 (en) * | 1999-11-05 | 2008-10-30 | Donald Kleinsek | Hair undifferentiated cells |
| US20080286242A2 (en) * | 1999-11-05 | 2008-11-20 | Donald Kleinsek | Augmentation and repair of spincter defects with cells including mesenchymal cells |
| CA2390053A1 (en) * | 1999-11-05 | 2001-05-10 | Gerigene Medical Corporation | Augmentation and repair of age-related soft tissue defects |
| EP1259634A4 (en) | 2000-03-02 | 2004-07-07 | Univ Rochester | EX VIVO CREATION OF FUNCTIONAL LEUKEMIA CELLS IN A THREE-DIMENSIONAL BIOREACTOR |
| EP2283880A3 (en) | 2000-03-20 | 2014-07-09 | Biosphere Medical, Inc. | Injectable microspheres for tissue construction |
| AU2001249221A1 (en) | 2000-03-20 | 2001-10-03 | Biosphere Medical, Inc. | Injectable and swellable microspheres for tissue bulking |
| US7338657B2 (en) * | 2001-03-15 | 2008-03-04 | Biosphere Medical, Inc. | Injectable microspheres for tissue construction |
| US6436424B1 (en) * | 2000-03-20 | 2002-08-20 | Biosphere Medical, Inc. | Injectable and swellable microspheres for dermal augmentation |
| US6638312B2 (en) | 2000-08-04 | 2003-10-28 | Depuy Orthopaedics, Inc. | Reinforced small intestinal submucosa (SIS) |
| US8366787B2 (en) * | 2000-08-04 | 2013-02-05 | Depuy Products, Inc. | Hybrid biologic-synthetic bioabsorbable scaffolds |
| EP1315796B1 (en) * | 2000-08-16 | 2006-07-12 | Duke University | Decellularized tissue engineered constructs and tissues |
| CA2457162A1 (en) | 2000-09-01 | 2002-03-07 | Virginia Commonwealth University Intellectual Property Foundation | Electroprocessed fibrin-based matrices and tissues |
| US20090130066A1 (en) * | 2000-11-06 | 2009-05-21 | Gerigene Medical Corporation | Augmentation and repair of sphincter defects with cells including muscle cells |
| JP4485124B2 (en) * | 2000-11-17 | 2010-06-16 | ヴァージニア コモンウェルス ユニバーシティ インテレクチュアル プロパティー ファンデーション | Electroprocessed collagen |
| AU2002220209B2 (en) | 2000-12-06 | 2006-05-25 | Robert J. Hariri | Method of collecting placental stem cells |
| US8057788B2 (en) | 2000-12-06 | 2011-11-15 | Anthrogenesis Corporation | Placental stem cell populations |
| US7311905B2 (en) | 2002-02-13 | 2007-12-25 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta, and uses and methods of treatment using said cells |
| US7041868B2 (en) | 2000-12-29 | 2006-05-09 | Kimberly-Clark Worldwide, Inc. | Bioabsorbable wound dressing |
| EP3246396B1 (en) | 2001-02-14 | 2020-01-29 | Celularity, Inc. | Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells |
| EP2336300B1 (en) | 2001-02-14 | 2015-07-08 | Anthrogenesis Corporation | Post-partum mammalian placenta, its use and placental stem cells therefrom |
| US20030032961A1 (en) * | 2001-07-16 | 2003-02-13 | Pelo Mark Joseph | Devices from naturally occurring biologically derived materials |
| US8025896B2 (en) | 2001-07-16 | 2011-09-27 | Depuy Products, Inc. | Porous extracellular matrix scaffold and method |
| AU2002316696B2 (en) * | 2001-07-16 | 2007-08-30 | Depuy Products, Inc. | Cartilage repair and regeneration scaffold and method |
| US7819918B2 (en) * | 2001-07-16 | 2010-10-26 | Depuy Products, Inc. | Implantable tissue repair device |
| US7361195B2 (en) * | 2001-07-16 | 2008-04-22 | Depuy Products, Inc. | Cartilage repair apparatus and method |
| JP4294474B2 (en) | 2001-07-16 | 2009-07-15 | デピュイ・プロダクツ・インコーポレイテッド | Meniscus reproduction device |
| US7163563B2 (en) | 2001-07-16 | 2007-01-16 | Depuy Products, Inc. | Unitary surgical device and method |
| US7201917B2 (en) | 2001-07-16 | 2007-04-10 | Depuy Products, Inc. | Porous delivery scaffold and method |
| US7914808B2 (en) | 2001-07-16 | 2011-03-29 | Depuy Products, Inc. | Hybrid biologic/synthetic porous extracellular matrix scaffolds |
| EP2301343A1 (en) | 2002-02-13 | 2011-03-30 | Anthrogenesis Corporation | Embryonic-like stem cells derived from post-partum mammalian placenta and uses and methods of treatment using said cells |
| WO2003072128A2 (en) * | 2002-02-22 | 2003-09-04 | Ebi, L.P. | Methods and compositions for treating bone or cartilage defects |
| US7498171B2 (en) | 2002-04-12 | 2009-03-03 | Anthrogenesis Corporation | Modulation of stem and progenitor cell differentiation, assays, and uses thereof |
| KR100527623B1 (en) * | 2002-06-01 | 2005-11-15 | 라이프코드인터내셔날 주식회사 | Biodegradable polymer scaffold containing extracellular matrix used for artificial organs and method for preparing same |
| JP2006509770A (en) | 2002-11-26 | 2006-03-23 | アンソロジェネシス コーポレーション | Cell therapy, cell therapy units, and therapies using them |
| IL156945A0 (en) * | 2003-07-15 | 2004-02-08 | Itzhak Tavori | Device and a method for orthopedic delivery of bone reconstruction medium |
| US7326571B2 (en) * | 2003-07-17 | 2008-02-05 | Boston Scientific Scimed, Inc. | Decellularized bone marrow extracellular matrix |
| JP2005211099A (en) * | 2004-01-27 | 2005-08-11 | Olympus Corp | Artificial organ component, manufacturing method of artificial organ formation and artificial organ |
| US7569233B2 (en) | 2004-05-04 | 2009-08-04 | Depuy Products, Inc. | Hybrid biologic-synthetic bioabsorbable scaffolds |
| EP1748744A4 (en) * | 2004-05-11 | 2011-08-03 | Synthasome Inc | Tissue scaffold |
| US20070190108A1 (en) * | 2004-05-17 | 2007-08-16 | Arindam Datta | High performance reticulated elastomeric matrix preparation, properties, reinforcement, and use in surgical devices, tissue augmentation and/or tissue repair |
| JP2008511672A (en) * | 2004-08-30 | 2008-04-17 | セレゲン，インコーポレーテッド | Cultured three-dimensional tissue and use thereof |
| US7513866B2 (en) | 2004-10-29 | 2009-04-07 | Depuy Products, Inc. | Intestine processing device and associated method |
| US7354627B2 (en) | 2004-12-22 | 2008-04-08 | Depuy Products, Inc. | Method for organizing the assembly of collagen fibers and compositions formed therefrom |
| CA2612188A1 (en) * | 2005-06-17 | 2007-01-04 | Theregen, Inc. | Methods for treating ischemic tissue |
| US7595062B2 (en) | 2005-07-28 | 2009-09-29 | Depuy Products, Inc. | Joint resurfacing orthopaedic implant and associated method |
| US20070065415A1 (en) * | 2005-09-16 | 2007-03-22 | Kleinsek Donald A | Compositions and methods for the augmentation and repair of defects in tissue |
| CA2974249C (en) | 2005-10-13 | 2021-10-12 | Anthrogenesis Corporation | Immunomodulation using placental stem cells |
| US20090035855A1 (en) * | 2005-12-01 | 2009-02-05 | Agency For Science, Technology And Research | Three-dimensional reconstituted extracellular matrices as scaffolds for tissue engineering |
| WO2007079184A2 (en) | 2005-12-29 | 2007-07-12 | Anthrogenesis Corporation | Co-culture of placental stem cells and stem cells from a second source |
| US20070178137A1 (en) * | 2006-02-01 | 2007-08-02 | Toby Freyman | Local control of inflammation |
| EP3483263A1 (en) | 2006-10-23 | 2019-05-15 | Celularity, Inc. | Methods and compositions for treatment of bone defects with placental cell populations |
| US7871440B2 (en) | 2006-12-11 | 2011-01-18 | Depuy Products, Inc. | Unitary surgical device and method |
| CA2677679A1 (en) | 2007-02-12 | 2008-08-21 | Anthrogenesis Corporation | Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations |
| AU2008216749B2 (en) | 2007-02-12 | 2014-03-13 | Celularity Inc. | Treatment of inflammatory diseases using placental stem cells |
| US9200253B1 (en) | 2007-08-06 | 2015-12-01 | Anthrogenesis Corporation | Method of producing erythrocytes |
| MX2010003291A (en) | 2007-09-28 | 2012-02-21 | Anthrogenesis Corp | Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells. |
| ES2667210T3 (en) | 2007-11-07 | 2018-05-10 | Anthrogenesis Corporation | Use of umbilical cord blood in the treatment of complications of premature delivery |
| ES2478678T3 (en) | 2007-11-30 | 2014-07-22 | Rnl Bio Co., Ltd. | Cellular therapeutic agent for urinary incontinence comprising stem cells originated from decidua |
| US10702554B2 (en) | 2007-12-03 | 2020-07-07 | Sanbio, Inc. | Extracellular matrix from pluripotent cells |
| AP3129A (en) | 2008-01-30 | 2015-02-28 | Histogen Inc | Extracellular matrix compositions |
| EP3357519B1 (en) * | 2008-07-02 | 2019-03-13 | Allergan, Inc. | Compositions for soft tissue filling and regeneration |
| MX339624B (en) | 2008-08-20 | 2016-06-02 | Anthrogenesis Corp | IMPROVED COMPOSITIONS OF CELLS AND METHODS TO PREPARE THE SAME. |
| BRPI0921249A8 (en) * | 2008-11-14 | 2018-12-04 | Histogen Inc | use of extracellular matrix in the preparation of a composition, use of extracellular matrix and chemotherapeutic agent in the preparation of a composition, and compositions for treating cancer |
| BRPI0922184A2 (en) | 2008-11-19 | 2020-08-18 | Anthrogenesis Corporation | isolated cell, isolated cell population, degradable permanent or decellularized matrix or synthetic structure, and method for treating an individual |
| ES2667265T3 (en) | 2009-07-10 | 2018-05-10 | Histogen, Inc. | Conditioned medium and extracellular matrix compositions of cells grown under hypoxic conditions |
| WO2011084925A2 (en) | 2010-01-05 | 2011-07-14 | Cell Constructs, Inc. | Biomaterials made from human hair |
| JP2013518108A (en) | 2010-01-26 | 2013-05-20 | アントフロゲネシス コーポレーション | Treatment of bone-related cancer using placental stem cells |
| TWI578993B (en) | 2010-04-07 | 2017-04-21 | 安瑟吉納西斯公司 | Using angiogenesis of placental stem cells |
| WO2012009422A1 (en) | 2010-07-13 | 2012-01-19 | Anthrogenesis Corporation | Methods of generating natural killer cells |
| EP2799077A1 (en) | 2010-11-12 | 2014-11-05 | Allergan, Inc. | Metabolized conditioned growth medium and methods of use |
| AR084754A1 (en) | 2010-12-30 | 2013-06-05 | Anthrogenesis Corp | COMPOSITIONS THAT INCLUDE MOTHER PLACENTA CELLS AND PLASMA RICH IN PLATES, AND THEIR METHODS OF USE |
| US20120171180A1 (en) | 2010-12-30 | 2012-07-05 | Sascha Abramson | Compositions comprising amnion derived adherent cells and platelet-rich plasma |
| WO2012092485A1 (en) | 2010-12-31 | 2012-07-05 | Anthrogenesis Corporation | Enhancement of placental stem cell potency using modulatory rna molecules |
| CA2822689C (en) | 2011-01-06 | 2015-12-29 | Humacyte | Tissue-engineered constructs |
| ES2707579T3 (en) | 2011-06-01 | 2019-04-04 | Celularity Inc | Pain treatment using placental cytoblast |
| EP3604555B1 (en) | 2011-10-14 | 2024-12-25 | President and Fellows of Harvard College | Sequencing by structure assembly |
| ES2991004T3 (en) | 2011-12-22 | 2024-12-02 | Harvard College | Methods for the detection of analytes |
| ES2953308T3 (en) | 2011-12-22 | 2023-11-10 | Harvard College | Compositions and methods for the detection of analytes |
| CN104303186B (en) * | 2012-05-09 | 2018-12-11 | 皇家飞利浦有限公司 | Intervene information agency medicine tracked interface |
| WO2013184754A2 (en) | 2012-06-05 | 2013-12-12 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using dna origami probes |
| JP2016506968A (en) | 2013-02-05 | 2016-03-07 | アントフロゲネシス コーポレーション | Placenta-derived natural killer cells |
| US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
| MX385330B (en) | 2013-06-04 | 2025-03-18 | Harvard College | RIBONUCLEIC ACID-GUIDED TRANSCRIPTIONAL REGULATION. |
| JP6882282B2 (en) | 2015-11-03 | 2021-06-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Methods and equipment for stereoscopic imaging of three-dimensional nucleic acid-containing matrices |
| AU2017257624B2 (en) | 2016-04-25 | 2023-05-25 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| CN105920679B (en) * | 2016-04-26 | 2018-10-19 | 青岛大学 | A kind of preparation method of the dermal scaffold material with three-dimensional gradient pore structure |
| EP4428536A3 (en) | 2016-08-31 | 2024-12-04 | President and Fellows of Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
| EP3507364A4 (en) | 2016-08-31 | 2020-05-20 | President and Fellows of Harvard College | METHOD FOR CREATING LIBRARIES OF NUCLEIC ACID SEQUENCES FOR DETECTION BY MEANS OF IN-SITU FLUORESCENCE SEQUENCING |
| US11185568B2 (en) | 2017-04-14 | 2021-11-30 | President And Fellows Of Harvard College | Methods for generation of cell-derived microfilament network |
| US11718829B2 (en) | 2017-07-28 | 2023-08-08 | Breakthrough Tech Llc | Methods and compositions for manufacturing extracellular matrix |
| CN112384200A (en) | 2018-01-30 | 2021-02-19 | 希斯托金公司 | Cell-derived extracellular vesicles cultured under hypoxic conditions and uses thereof |
| WO2020028194A1 (en) | 2018-07-30 | 2020-02-06 | Readcoor, Inc. | Methods and systems for sample processing or analysis |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN111084788A (en) * | 2018-10-22 | 2020-05-01 | 上海蓝盎电子科技发展有限公司 | Artificial intercellular substance |
| US12077780B2 (en) | 2020-02-14 | 2024-09-03 | Allergan Sales, Llc | Conditioned medium from cells cultured under hypoxic conditions and uses thereof |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| EP4395796A4 (en) | 2021-09-01 | 2024-12-25 | Shanghai Qisheng Biological Preparation Co., Ltd. | CARTILAGE REGENERATION USING IN SITU POLYMERIZABLE INJECTABLE COLLAGEN COMPOSITIONS CONTAINING CHONDROCYTES OR STEM CELLS |
| CN115006597B (en) * | 2022-06-02 | 2024-01-19 | 上海威高医疗技术发展有限公司 | Oral cavity repairing film and preparation method thereof |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3949073A (en) * | 1974-11-18 | 1976-04-06 | The Board Of Trustees Of Leland Stanford Junior University | Process for augmenting connective mammalian tissue with in situ polymerizable native collagen solution |
| US4424208A (en) * | 1982-01-11 | 1984-01-03 | Collagen Corporation | Collagen implant material and method for augmenting soft tissue |
| US4582640A (en) * | 1982-03-08 | 1986-04-15 | Collagen Corporation | Injectable cross-linked collagen implant material |
| US4837285A (en) * | 1984-03-27 | 1989-06-06 | Medimatrix | Collagen matrix beads for soft tissue repair |
| US5288617A (en) * | 1984-10-31 | 1994-02-22 | Commonwealth Scientific And Industrial Research Organization | Method of producing an antigenic preparation |
| US4963489A (en) * | 1987-04-14 | 1990-10-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US5266480A (en) * | 1986-04-18 | 1993-11-30 | Advanced Tissue Sciences, Inc. | Three-dimensional skin culture system |
| US4721096A (en) * | 1986-04-18 | 1988-01-26 | Marrow-Tech Incorporated | Process for replicating bone marrow in vitro and using the same |
| US5160490A (en) * | 1986-04-18 | 1992-11-03 | Marrow-Tech Incorporated | Three-dimensional cell and tissue culture apparatus |
| US5460939A (en) * | 1986-04-18 | 1995-10-24 | Advanced Tissue Sciences, Inc. | Temporary living skin replacement |
| US5032508A (en) * | 1988-09-08 | 1991-07-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US4803075A (en) * | 1986-06-25 | 1989-02-07 | Collagen Corporation | Injectable implant composition having improved intrudability |
| JPH041118Y2 (en) * | 1986-07-31 | 1992-01-14 | ||
| GB8708009D0 (en) * | 1987-04-03 | 1987-05-07 | Clayton Found Res | Injectable soft tissue augmentation materials |
| US5342765A (en) * | 1991-08-01 | 1994-08-30 | Occidental Chemical Corporation | Method of producing extracellular products from aerobic microorganisms |
| US5204382A (en) * | 1992-02-28 | 1993-04-20 | Collagen Corporation | Injectable ceramic compositions and methods for their preparation and use |
| US5478739A (en) * | 1992-10-23 | 1995-12-26 | Advanced Tissue Sciences, Inc. | Three-dimensional stromal cell and tissue culture system |
| US5541106A (en) * | 1993-04-05 | 1996-07-30 | Desmos, Inc. | Cell matrix stimulated attachment and hemidesmosome assembly |
| US5397352A (en) * | 1993-08-27 | 1995-03-14 | Burres; Steven | Method of recollagenation |
| US5516532A (en) * | 1994-08-05 | 1996-05-14 | Children's Medical Center Corporation | Injectable non-immunogenic cartilage and bone preparation |
-
1995
- 1995-06-06 US US08/470,101 patent/US5830708A/en not_active Expired - Lifetime
-
1996
- 1996-06-06 AT AT96921369T patent/ATE290350T1/en not_active IP Right Cessation
- 1996-06-06 DE DE69634454T patent/DE69634454T2/en not_active Expired - Lifetime
- 1996-06-06 WO PCT/US1996/009482 patent/WO1996039101A1/en active Search and Examination
- 1996-06-06 CA CA002223892A patent/CA2223892A1/en not_active Abandoned
- 1996-06-06 JP JP50179097A patent/JP3709487B2/en not_active Expired - Lifetime
- 1996-06-06 AU AU62609/96A patent/AU708090B2/en not_active Expired
- 1996-06-06 KR KR1019970708695A patent/KR19990022216A/en not_active Ceased
- 1996-06-06 ES ES96921369T patent/ES2238076T3/en not_active Expired - Lifetime
- 1996-06-06 EP EP96921369A patent/EP0967934B1/en not_active Expired - Lifetime
- 1996-06-06 NZ NZ311313A patent/NZ311313A/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO1996039101B1 (en) | Compositions and methods for naturally secreted extracellular matrix | |
| DE69634454T2 (en) | Compositions and methods relating to a naturally secreted matrix | |
| DE69531638T2 (en) | BIOMATRIX FOR TISSUE REGENATION | |
| DE69626035T2 (en) | A BIOLOGICAL MATERIAL CONTAINING AN EFFECTIVE CULTURE OF BONE MARROW STAMPS DIFFERENTIATED PARTIALLY OR COMPLETELY IN BINDING WEB CELLS, AND A THREE-DIMENSIONAL, BIODEGRADABLE AND BIODEGRADABLE MATRIX THAT CONSISTS OF A HYALURONIC ACID DERIVATIVE | |
| US6284284B1 (en) | Compositions and methods for production and use of an injectable naturally secreted extracellular matrix | |
| US8864844B2 (en) | Tissue scaffold | |
| DE3751843T2 (en) | MANUFACTURE OF BODY ORGANS BY CONTROLLED CELL GROWTH ON ARTIFICIAL MATRIX | |
| DE60022075T2 (en) | MECHANICAL HEART FLAP AND ITS MANUFACTURING METHOD | |
| DE69724275T2 (en) | BIOPOLYMER FOAMS FOR TISSUE RENEWAL AND RECONSTRUCTION | |
| DE69534083T2 (en) | BREAST TISSUE TECHNOLOGY | |
| DE69028524T2 (en) | IN VIVO-NEOMORPHOGENESE BY CARTILAGE FROM CELL CULTURES | |
| DE69017820T2 (en) | IMPLANTS FOR LARGE QUANTITIES OF CELLS ON POLYMERIC MATRICES. | |
| DE60018576T2 (en) | IN VITRO HEALING OF BONE AND / OR TROUBLE DAMAGE | |
| US5665391A (en) | Cultured, full-thickness integument substitutes based on three-dimensional matrix membranes | |
| Yannas et al. | Surface biology of collagen scaffold explains blocking of wound contraction and regeneration of skin and peripheral nerves | |
| DE69803065T2 (en) | CELL CULTURE MATRIX AND BIODEGRADABLE THREE-DIMENSIONAL MATRIX ON A HYALURONIC ACID DERIVATIVE BASE | |
| CN101998862A (en) | Collagen products and methods for producing collagen products | |
| AU2009212946A1 (en) | Autologous cells on a support matrix for tissue repair | |
| DE69913543T3 (en) | Dermal scaffold based on a neutralized chitosan sponge or a neutralized mixed chitosan-collagen sponge | |
| DE112012005018B4 (en) | Process for producing auricular cartilage tissue and auricular cartilage tissue | |
| Yannas | Applications of ECM analogs in surgery | |
| EP1196206A1 (en) | Human naturally secreted extracellular matrix-coated device | |
| CN110331127A (en) | A kind of preparation method of organizational project Skin scar model | |
| EP1184040A1 (en) | Skin matrix for covering and regeneration of injured skin parts and process for making the same | |
| WO2024037293A1 (en) | Cellular matrix nerve graft for repairing peripheral nerve injury and preparation method therefor |