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WO1996039195A3 - Chemically modified oligonucleotide for site-directed mutagenesis - Google Patents

Chemically modified oligonucleotide for site-directed mutagenesis Download PDF

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Publication number
WO1996039195A3
WO1996039195A3 PCT/US1996/008883 US9608883W WO9639195A3 WO 1996039195 A3 WO1996039195 A3 WO 1996039195A3 US 9608883 W US9608883 W US 9608883W WO 9639195 A3 WO9639195 A3 WO 9639195A3
Authority
WO
WIPO (PCT)
Prior art keywords
chemically modified
site
directed mutagenesis
modified oligonucleotide
molecule
Prior art date
Application number
PCT/US1996/008883
Other languages
French (fr)
Other versions
WO1996039195A2 (en
Inventor
Peter M Glazer
Pamela A Havre
Original Assignee
Univ Yale
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Yale filed Critical Univ Yale
Priority to AU60432/96A priority Critical patent/AU6043296A/en
Publication of WO1996039195A2 publication Critical patent/WO1996039195A2/en
Publication of WO1996039195A3 publication Critical patent/WO1996039195A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3511—Conjugate intercalating or cleaving agent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)

Abstract

A mutagenic, triplex-forming oligonucleotide and methods for use thereof wherein the oligonucleotide is chemically modified to incorporate a mutagen and forms a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the mutagen is brought into proximity with the target molecule and causes a mutation at a specific site therein. The mutation activates, inactivates or alters the activity and function of the target molecule.
PCT/US1996/008883 1995-06-06 1996-06-04 Chemically modified oligonucleotide for site-directed mutagenesis WO1996039195A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU60432/96A AU6043296A (en) 1995-06-06 1996-06-04 Chemically modified oligonucleotide for site-directed mutage nesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46351995A 1995-06-06 1995-06-06
US463,519 1995-06-06

Publications (2)

Publication Number Publication Date
WO1996039195A2 WO1996039195A2 (en) 1996-12-12
WO1996039195A3 true WO1996039195A3 (en) 1997-01-30

Family

ID=23840376

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/008883 WO1996039195A2 (en) 1995-06-06 1996-06-04 Chemically modified oligonucleotide for site-directed mutagenesis

Country Status (2)

Country Link
AU (1) AU6043296A (en)
WO (1) WO1996039195A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136601A (en) * 1991-08-21 2000-10-24 Epoch Pharmaceuticals, Inc. Targeted mutagenesis in living cells using modified oligonucleotides
US11918695B2 (en) 2014-05-09 2024-03-05 Yale University Topical formulation of hyperbranched polymer-coated particles
ES3007997T3 (en) 2014-05-09 2025-03-21 Univ Yale Hyperbranched polyglycerol-coated particles
CA3014795A1 (en) 2016-02-16 2017-08-24 Yale University Compositions and methods for treatment of cystic fibrosis
US11136597B2 (en) 2016-02-16 2021-10-05 Yale University Compositions for enhancing targeted gene editing and methods of use thereof
US20200113821A1 (en) 2017-04-04 2020-04-16 Yale University Compositions and methods for in utero delivery
US20210189431A1 (en) 2018-08-10 2021-06-24 Yale University Compositions and methods for embryonic gene editing in vitro
CN112912502A (en) 2018-08-31 2021-06-04 耶鲁大学 Compositions and methods for enhancing triplex- and nuclease-based gene editing
WO2020112195A1 (en) 2018-11-30 2020-06-04 Yale University Compositions, technologies and methods of using plerixafor to enhance gene editing
WO2020257776A1 (en) 2019-06-21 2020-12-24 Yale University Peptide nucleic acid compositions with modified hoogsteen binding segments and methods of use thereof
US20220372474A1 (en) 2019-06-21 2022-11-24 Yale University Hydroxymethyl-modified gamma-pna compositions and methods of use thereof
US20220339294A1 (en) 2019-09-09 2022-10-27 Yale University Nanoparticles for selective tissue or cellular uptake

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0253193A2 (en) * 1986-07-08 1988-01-20 Abbott Laboratories Method for mutagenesis by oligonucleotide-directed repair of a strand break
US5354670A (en) * 1991-12-24 1994-10-11 The President And Fellows Of Harvard College Site-directed mutagenesis of DNA
WO1995001364A1 (en) * 1993-06-25 1995-01-12 Yale University Chemically modified oligonucleotide for site-directed mutagenesis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0253193A2 (en) * 1986-07-08 1988-01-20 Abbott Laboratories Method for mutagenesis by oligonucleotide-directed repair of a strand break
US5354670A (en) * 1991-12-24 1994-10-11 The President And Fellows Of Harvard College Site-directed mutagenesis of DNA
WO1995001364A1 (en) * 1993-06-25 1995-01-12 Yale University Chemically modified oligonucleotide for site-directed mutagenesis

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GASPARRO F P ET AL: "Site-specific targeting of psoralen photoadducts with a triple helix-forming oligonucleotide: Characterization of psoralen monoadduct and crosslink formation.", NUCLEIC ACIDS RESEARCH 22 (14). 1994. 2845-2852. ISSN: 0305-1048, XP002018568 *
HAVRE P A ET AL: "Targeted mutagenesis of simian virus 40 DNA mediated by a triple helix-forming oligonucleotide.", JOURNAL OF VIROLOGY 67 (12). 1993. 7324-7331. ISSN: 0022-538X, XP002018570 *
HAVRE PA ET AL: "Targeted mutagenesis of DNA using triple helix-forming oligonucleotides linked to psoralen.", PROC NATL ACAD SCI U S A, AUG 15 1993, 90 (16) P7879-83, UNITED STATES, XP002018569 *
RAHA M ET AL: "Mutagenesis by third-strand-directed psoralen adducts in repair-deficient human cells: high frequency and altered spectrum in a xeroderma pigmentosum variant.", PROC NATL ACAD SCI U S A, APR 2 1996, 93 (7) P2941-6, UNITED STATES, XP002018566 *
WANG G ET AL: "Targeted mutagenesis in mammalian cells mediated by intracellular triple helix formation.", MOLECULAR AND CELLULAR BIOLOGY 15 (3). 1995. 1759-1768. ISSN: 0270-7306, XP002018567 *

Also Published As

Publication number Publication date
AU6043296A (en) 1996-12-24
WO1996039195A2 (en) 1996-12-12

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