WO1996015264B1 - Procede d'identification de sequences nucleotidiques a partir de differentes sources biologiques par amplification des sequences - Google Patents
Procede d'identification de sequences nucleotidiques a partir de differentes sources biologiques par amplification des sequencesInfo
- Publication number
- WO1996015264B1 WO1996015264B1 PCT/GB1995/002654 GB9502654W WO9615264B1 WO 1996015264 B1 WO1996015264 B1 WO 1996015264B1 GB 9502654 W GB9502654 W GB 9502654W WO 9615264 B1 WO9615264 B1 WO 9615264B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- seq
- fragment
- sequence
- amplification
- Prior art date
Links
- 150000007523 nucleic acids Chemical group 0.000 title claims abstract 16
- 238000000034 method Methods 0.000 title claims abstract 12
- 230000003321 amplification Effects 0.000 title claims abstract 10
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract 10
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract 10
- 239000013615 primer Substances 0.000 claims abstract 10
- 239000003155 DNA primer Substances 0.000 claims abstract 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 5
- 239000007795 chemical reaction product Substances 0.000 claims abstract 4
- 239000012472 biological sample Substances 0.000 claims abstract 3
- 108090000623 proteins and genes Proteins 0.000 claims abstract 3
- 102000004169 proteins and genes Human genes 0.000 claims abstract 2
- 239000012634 fragment Substances 0.000 claims 10
- 239000002773 nucleotide Substances 0.000 claims 10
- 125000003729 nucleotide group Chemical group 0.000 claims 10
- 108091034117 Oligonucleotide Proteins 0.000 claims 9
- 150000001413 amino acids Chemical class 0.000 claims 8
- 230000000295 complement effect Effects 0.000 claims 6
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 108010049152 Cold Shock Proteins and Peptides Proteins 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 230000035939 shock Effects 0.000 abstract 1
Abstract
Procédé d'identification d'une séquence nucléotidique dans un échantillon biologique consistant à utiliser une paire d'amorces oligonucléotidiques universelles pour amplifier la séquence nuclótidique et à caractériser les produits de réaction de l'amplification. Les amorces universelles préférées sont dérivées de régions conservées de protéines du type 'choc froid' ou de protéines de séquence Y et s'hybrident avec les gènes qui codent les séquences peptidiques GXVKWFNXXKGFGFI et GPXAXNVTXX.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/666,493 US5948649A (en) | 1994-11-12 | 1995-11-13 | Method for identifying nucleic acid sequences from different biological sources through amplification of the same |
| EP95936670A EP0731850A1 (fr) | 1994-11-12 | 1995-11-13 | Procede d'identification de sequences nucleotidiques a partir de differentes sources biologiques par amplification des sequences |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9422891A GB9422891D0 (en) | 1994-11-12 | 1994-11-12 | Improvements in and relating to the amplification and identification of nucleotide sequences |
| GB9422891.3 | 1994-11-12 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO1996015264A2 WO1996015264A2 (fr) | 1996-05-23 |
| WO1996015264A3 WO1996015264A3 (fr) | 1996-08-29 |
| WO1996015264B1 true WO1996015264B1 (fr) | 1996-10-03 |
Family
ID=10764324
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/GB1995/002654 WO1996015264A2 (fr) | 1994-11-12 | 1995-11-13 | Procede d'identification de sequences nucleotidiques a partir de differentes sources biologiques par amplification des sequences |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US5948649A (fr) |
| EP (1) | EP0731850A1 (fr) |
| GB (1) | GB9422891D0 (fr) |
| WO (1) | WO1996015264A2 (fr) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9615516D0 (en) * | 1996-07-24 | 1996-09-04 | Jsd Tech Ltd | Bacillus cereus |
| US7534568B2 (en) | 1999-10-29 | 2009-05-19 | Hologic Inc. | Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe |
| US7118860B2 (en) * | 1999-10-29 | 2006-10-10 | Stratagene California | Methods for detection of a target nucleic acid by capture |
| US6528254B1 (en) * | 1999-10-29 | 2003-03-04 | Stratagene | Methods for detection of a target nucleic acid sequence |
| WO2001048242A2 (fr) | 1999-12-29 | 2001-07-05 | Mergen Ltd. | Procedes d'amplification et de detection de plusieurs polynucleotides sur un support en phase solide |
| WO2001051661A2 (fr) * | 2000-01-13 | 2001-07-19 | Amsterdam Support Diagnostics B.V. | Systeme universel d'amplification de l'acide nucleique pour acides nucleiques d'un prelevement |
| US6376191B1 (en) | 2000-03-22 | 2002-04-23 | Mergen, Ltd. | Microarray-based analysis of polynucleotide sequence variations |
| EP1418241A1 (fr) * | 2002-11-08 | 2004-05-12 | PrimaGen Holding B.V. | Procédé de quantifier le rapport de deux séquences d'acides nucléiques |
| US8206913B1 (en) | 2003-03-07 | 2012-06-26 | Rubicon Genomics, Inc. | Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process |
| DE602004029560D1 (de) | 2003-03-07 | 2010-11-25 | Rubicon Genomics Inc | Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden |
| WO2005090607A1 (fr) | 2004-03-08 | 2005-09-29 | Rubicon Genomics, Inc. | Procedes et compositions pour la generation et l'amplification de bibliotheques d'adn pour la detection et l'analyse sensible de methylation d'adn |
| ATE510930T1 (de) | 2005-08-02 | 2011-06-15 | Rubicon Genomics Inc | Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion |
| WO2007018602A1 (fr) | 2005-08-02 | 2007-02-15 | Rubicon Genomics, Inc. | Isolement des ilots cpg au moyen d'un procede de segregation thermique et de selection-amplification enzymatique |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| FR2596774B1 (fr) * | 1986-04-04 | 1988-07-22 | Pasteur Institut | Sondes oligonucleotidiques et procedes pour la detection par hybridation des acides nucleiques de bacteries et autres etres vivants |
| FR2636075B1 (fr) * | 1988-09-07 | 1991-11-15 | Biotechnologie Ste Europ | Procede de detection des bacteries, levures, parasites et autres eucaryotes, notamment dans les produits alimentaires |
| CA2033718A1 (fr) * | 1990-01-19 | 1991-07-20 | Ronald M. Atlas | Procede de detection d'agents pathogenes microbiens dans l'eau et indicateurs de contamination par des matieres fecales humaines dans des echantillons d'eau et trousses de detection connexes |
| CA2026264A1 (fr) * | 1990-09-26 | 1992-03-27 | William Scott Davidson | Test pour determiner l'identite de l'espece et(ou) de la population d'un organisme par l'analyse directe de la sequence des nucleotides de segments definis du genome |
| US5470971A (en) * | 1991-03-11 | 1995-11-28 | The University Of Medicine And Dentistry Of New Jersey | Stress-induced proteins, genes coding therefor, transformed cells of organisms, methods and applications |
| US6232061B1 (en) * | 1992-04-01 | 2001-05-15 | Cambridge Neuroscience, Inc. | Homology cloning |
-
1994
- 1994-11-12 GB GB9422891A patent/GB9422891D0/en active Pending
-
1995
- 1995-11-13 WO PCT/GB1995/002654 patent/WO1996015264A2/fr not_active Application Discontinuation
- 1995-11-13 US US08/666,493 patent/US5948649A/en not_active Expired - Fee Related
- 1995-11-13 EP EP95936670A patent/EP0731850A1/fr not_active Withdrawn
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