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WO1996015259A3 - Assay for the measurement of multilineage stem cells and methods of use thereof - Google Patents

Assay for the measurement of multilineage stem cells and methods of use thereof Download PDF

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Publication number
WO1996015259A3
WO1996015259A3 PCT/EP1995/004480 EP9504480W WO9615259A3 WO 1996015259 A3 WO1996015259 A3 WO 1996015259A3 EP 9504480 W EP9504480 W EP 9504480W WO 9615259 A3 WO9615259 A3 WO 9615259A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
stem cells
population
hematopoietic
assay
Prior art date
Application number
PCT/EP1995/004480
Other languages
French (fr)
Other versions
WO1996015259A2 (en
Inventor
Amittha Wickrema
Original Assignee
Sandoz Ltd
Sandoz Ag
Systemix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sandoz Ltd, Sandoz Ag, Systemix Inc filed Critical Sandoz Ltd
Priority to AU41167/96A priority Critical patent/AU4116796A/en
Publication of WO1996015259A2 publication Critical patent/WO1996015259A2/en
Publication of WO1996015259A3 publication Critical patent/WO1996015259A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention encompasses methods of detecting and quantitating hematopoietic stem cells in a population of hematopoietic cells. A population of hematopoietic cells is first obtained and treated to enrich for stem cells. Single cells in the enriched population are deposited onto a suitable growth substrate such as a well in a 96 well microtiter plate. The single cell deposits are cultured under conditions suitable to maintain viability and induce differentiation. The resultant populations are then assayed for the expression of lineage-specific messenger RNA transcripts specific to at least two different lineages. The presence of transcripts specific to two or more different lineages is scored as positive for the initial presence of a stem cell.
PCT/EP1995/004480 1994-11-14 1995-11-14 Assay for the measurement of multilineage stem cells and methods of use thereof WO1996015259A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU41167/96A AU4116796A (en) 1994-11-14 1995-11-14 Assay for the measurement of multilineage stem cells and methods of use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33998594A 1994-11-14 1994-11-14
US08/339,985 1994-11-14

Publications (2)

Publication Number Publication Date
WO1996015259A2 WO1996015259A2 (en) 1996-05-23
WO1996015259A3 true WO1996015259A3 (en) 1996-09-19

Family

ID=23331407

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1995/004480 WO1996015259A2 (en) 1994-11-14 1995-11-14 Assay for the measurement of multilineage stem cells and methods of use thereof

Country Status (2)

Country Link
AU (1) AU4116796A (en)
WO (1) WO1996015259A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL154159A0 (en) 2000-08-01 2003-07-31 Yissum Res Dev Co Directed differentiation of ebryonic cells
WO2005030999A1 (en) * 2003-09-25 2005-04-07 Dana-Farber Cancer Institute, Inc Methods to detect lineage-specific cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994008039A1 (en) * 1992-10-02 1994-04-14 Systemix, Inc. Method of enrichment for human hematopoietic stem cells using c-kit

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994008039A1 (en) * 1992-10-02 1994-04-14 Systemix, Inc. Method of enrichment for human hematopoietic stem cells using c-kit

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MOUTHON MA ET AL: "Expression of tal-1 and GATA-binding proteins during human hematopoiesis.", BLOOD, FEB 1 1993, 81 (3) P647-55, UNITED STATES, XP000564653 *
THOMA S J ET AL: "Phenotype analysis of hematopoietic CD34+ cell populations derived from human umbilical cord blood using flow cytometry and cDNA-polymerase chain reaction", BLOOD, 83 (8). 1994. 2103-2114., XP000564654 *

Also Published As

Publication number Publication date
WO1996015259A2 (en) 1996-05-23
AU4116796A (en) 1996-06-06

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