WO1996015252A3 - Vector and methods for introducing at least two genes into a plant - Google Patents
Vector and methods for introducing at least two genes into a plant Download PDFInfo
- Publication number
- WO1996015252A3 WO1996015252A3 PCT/JP1995/002283 JP9502283W WO9615252A3 WO 1996015252 A3 WO1996015252 A3 WO 1996015252A3 JP 9502283 W JP9502283 W JP 9502283W WO 9615252 A3 WO9615252 A3 WO 9615252A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gene
- plant
- introducing
- vector
- genes
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Abstract
Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
UA97062658A UA40661C2 (en) | 1994-11-09 | 1995-11-08 | vector for incorporation of desired gene into the plant (variants), a method for obtaining transgenic plant, a method for incorporation of at least two DESIRED genes into the plant |
BRPI9509715-5A BR9509715B1 (en) | 1994-11-09 | 1995-11-08 | vector, process for transgenic plant production and process for introducing at least two genes into a plant. |
RU97109836A RU2149187C1 (en) | 1994-11-09 | 1995-11-08 | Vector for insertion of required gene in plant (variants), method of transgenic plant producing and method of insertion of at least two required genes in plant |
AU38557/95A AU703485B2 (en) | 1994-11-09 | 1995-11-08 | Vector and methods for introducing at least two genes into plant |
NZ295256A NZ295256A (en) | 1994-11-09 | 1995-11-08 | Vector containing a desired gene, a selectable marker gene and a removable dna element wherein the selectable marker gene is removed along with the removable dna element once it has been expressed |
APAP/P/1997/000980A AP9700980A0 (en) | 1994-11-09 | 1995-11-08 | Vector and methods for introducing at least two genes into a plant. |
PL95320201A PL184707B1 (en) | 1994-11-09 | 1995-11-08 | Vectors for and methods of introducing at least two genes into a plant organism |
SK569-97A SK56997A3 (en) | 1994-11-09 | 1995-11-08 | Vector and methods for introducing at least two genes into a plant |
NO972108A NO972108L (en) | 1994-11-09 | 1997-05-07 | Vector and methods for introducing at least two genes into a plant |
FI971961A FI971961L (en) | 1994-11-09 | 1997-05-07 | Vector and methods for transferring at least two genes into a plant |
BG101524A BG62892B1 (en) | 1994-11-09 | 1997-05-29 | Vector and methods for introducing at least two genes into a plant |
Applications Claiming Priority (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP31139994 | 1994-11-09 | ||
JP6/311399 | 1994-11-09 | ||
JP17012395 | 1995-05-31 | ||
JP7/170123 | 1995-05-31 | ||
JP7/293254 | 1995-10-04 | ||
JP29325495 | 1995-10-04 | ||
JP31343295A JP3256952B2 (en) | 1994-11-09 | 1995-10-25 | Vector for gene transfer into plant, method for producing transgenic plant using the same, and method for multiple gene transfer into plant |
JP7/313432 | 1995-10-25 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1996015252A2 WO1996015252A2 (en) | 1996-05-23 |
WO1996015252A3 true WO1996015252A3 (en) | 1996-07-18 |
Family
ID=27474306
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP1995/002283 WO1996015252A2 (en) | 1994-11-09 | 1995-11-08 | Vector and methods for introducing at least two genes into a plant |
Country Status (25)
Country | Link |
---|---|
US (1) | US5965791A (en) |
EP (2) | EP1602729A3 (en) |
JP (1) | JP3256952B2 (en) |
KR (1) | KR100343759B1 (en) |
CN (1) | CN1073624C (en) |
AP (1) | AP9700980A0 (en) |
AR (1) | AR000479A1 (en) |
AT (1) | ATE307211T1 (en) |
AU (1) | AU703485B2 (en) |
BG (1) | BG62892B1 (en) |
BR (1) | BR9509715B1 (en) |
CA (1) | CA2162449C (en) |
CZ (1) | CZ138897A3 (en) |
DE (1) | DE69534525D1 (en) |
DK (1) | DK0716147T3 (en) |
ES (1) | ES2248795T3 (en) |
FI (1) | FI971961L (en) |
HU (1) | HUT77074A (en) |
NO (1) | NO972108L (en) |
NZ (1) | NZ295256A (en) |
OA (1) | OA10607A (en) |
PL (1) | PL184707B1 (en) |
SK (1) | SK56997A3 (en) |
UA (1) | UA40661C2 (en) |
WO (1) | WO1996015252A2 (en) |
Families Citing this family (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AUPN903196A0 (en) * | 1996-03-29 | 1996-04-26 | Australian National University, The | Single-step excision means |
CN1195064C (en) * | 1996-05-09 | 2005-03-30 | 日本制纸株式会社 | Vector for gene transfer into plant allowing optional deletion of marker gene |
US6204434B1 (en) | 1996-09-11 | 2001-03-20 | Genesis Research & Development Corporation Limited | Materials and methods for the modification of plant lignin content |
US5850020A (en) * | 1996-09-11 | 1998-12-15 | Genesis Research & Development Corporation, Ltd. | Materials and method for the modification of plant lignin content |
US6410718B1 (en) | 1996-09-11 | 2002-06-25 | Genesis Research & Development Corporation Ltd. | Materials and methods for the modification of plant lignin content |
FR2759857B1 (en) * | 1997-02-27 | 1999-04-30 | Biocem | NEW USES OF MALE STERILITY IN PLANTS |
US6632980B1 (en) * | 1997-10-24 | 2003-10-14 | E. I. Du Pont De Nemours And Company | Binary viral expression system in plants |
JP2000083680A (en) * | 1998-07-16 | 2000-03-28 | Nippon Paper Industries Co Ltd | Introduction of gene into plant utilizing adventitious bud redifferentiation gene put under control due to photoinduction type promoter as selection marker gene and vector for transduction of gene into plant used therefor |
FR2784393B1 (en) * | 1998-10-09 | 2002-02-22 | Biogemma Fr | PROCESS FOR OBTAINING TRANSGENIC PLANTS EXPRESSING A PROTEIN HAVING HYDROGEN PEROXIDE PRODUCING BY TRANSFORMATION BY AGROBACTERIUM RHIZOGENES |
ATE358731T1 (en) | 1998-10-09 | 2007-04-15 | Arborgen Llc | POLYPEPTIDES WITH 4-CUMARATE:COA LIGASE ACTIVITY AND USES THEREOF. |
CA2292798A1 (en) | 1999-01-06 | 2000-07-06 | Nippon Paper Industries Co., Ltd. | Novel vector for introducing a gene into a plant using a selectable marker |
IL146338A0 (en) | 1999-05-17 | 2002-07-25 | Icon Genetics Inc | Process of rapid variety-independent plant transformation |
US6231561B1 (en) | 1999-09-20 | 2001-05-15 | Appriva Medical, Inc. | Method and apparatus for closing a body lumen |
US6723896B1 (en) * | 1999-11-12 | 2004-04-20 | The Rockefeller University | Inducible site-specific recombination for the activation and removal of transgenes in transgenic plants |
US7112721B2 (en) | 2000-02-08 | 2006-09-26 | Sakata Seed Corporation | Methods and constructs for plant transformation |
EP1261725A2 (en) * | 2000-02-08 | 2002-12-04 | Sakata Seed Corporation | Methods and constructs for agrobacterium-mediated plant transformation |
US6750379B2 (en) | 2000-03-09 | 2004-06-15 | Dekalb Genetics Corporation | Homologous recombination-mediated transgene alterations in plants |
US6512165B1 (en) * | 2000-07-10 | 2003-01-28 | Pioneer Hi-Bred International, Inc. | Methods for enhancing plant transformation frequencies |
US7560622B2 (en) * | 2000-10-06 | 2009-07-14 | Pioneer Hi-Bred International, Inc. | Methods and compositions relating to the generation of partially transgenic organisms |
US6927322B2 (en) | 2001-03-30 | 2005-08-09 | Cornell Research Foundation, Inc. | Cabbage proteinase inhibitor gene confers resistance against plant pests |
EP1264891A1 (en) * | 2001-05-31 | 2002-12-11 | Plant Research International B.V. | Modification of plant genomes by inducible site-specific recombination of transgenes |
JP3985474B2 (en) * | 2001-07-24 | 2007-10-03 | 日本製紙株式会社 | Gene transfer method to Eucalyptus plants with improved gene transfer efficiency |
EP1279737A1 (en) | 2001-07-27 | 2003-01-29 | Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. | Transformation method for obtaining marker-free plants |
JPWO2003040363A1 (en) * | 2001-11-08 | 2005-03-03 | 独立行政法人科学技術振興機構 | Rice transposon gene |
US7534934B2 (en) | 2002-02-20 | 2009-05-19 | J.R. Simplot Company | Precise breeding |
NZ535395A (en) * | 2002-02-20 | 2009-01-31 | Simplot Co J R | Precise plant breeding using plant DNA border like sequences instead of Agrobacterium borders |
JP4102921B2 (en) * | 2002-07-26 | 2008-06-18 | 日本製紙株式会社 | Efficient production method of transgenic plant using auxin precursor |
CA2493364A1 (en) | 2002-07-26 | 2004-02-12 | Basf Plant Science Gmbh | Inversion of the negative-selective effect of negative marker proteins using selection methods |
WO2004076625A2 (en) * | 2003-02-21 | 2004-09-10 | Vector Tobacco Ltd. | Method of identifying plant cells transformed with a gene |
AU2004225649B2 (en) | 2003-03-28 | 2008-11-06 | National Institute Of Agrobiological Sciences | Process for producing plant storage organ with high production of recombinant protein and novel recombinant protein |
US7575917B2 (en) | 2003-04-09 | 2009-08-18 | Monsanto Technology Llc | DNA constructs and methods to enhance the production of commercially viable transgenic plants |
EP1699926B1 (en) | 2003-12-10 | 2014-06-18 | Monsanto Technology, LLC | Stress tolerant plants and methods thereof |
AU2007249224B2 (en) | 2006-05-12 | 2013-03-21 | Monsanto Technology Llc | Methods and compositions for obtaining marker-free transgenic plants |
EP1949785A1 (en) | 2007-01-26 | 2008-07-30 | Coöperatie AVEBE U.A. | Use of R-genes as a selection marker in plant transformation and use of cisgenes in plant transformation |
WO2010009353A1 (en) | 2008-07-16 | 2010-01-21 | Monsanto Technology Llc | Methods and vectors for producing transgenic plants |
US7868688B2 (en) * | 2008-12-30 | 2011-01-11 | Cosmic Circuits Private Limited | Leakage independent very low bandwith current filter |
US11608505B2 (en) | 2017-11-27 | 2023-03-21 | Riken | Genome-edited plant production method |
CN108753813B (en) * | 2018-06-08 | 2021-08-24 | 中国水稻研究所 | Methods of obtaining marker-free transgenic plants |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0334383A2 (en) * | 1988-03-25 | 1989-09-27 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Transgenic plant with modified physiology, morphology and modified hormone metabolism, tissue cultures of this plant and process for its preparation |
WO1992001370A1 (en) * | 1990-07-19 | 1992-02-06 | The Regents Of The University Of California | Biologically safe plant transformation system |
WO1994017176A1 (en) * | 1993-01-29 | 1994-08-04 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5512482A (en) | 1990-04-26 | 1996-04-30 | Calgene, Inc. | Plant thioesterases |
GB9024728D0 (en) | 1990-11-14 | 1991-01-02 | Ici Plc | Herbicide resistant plants |
BR9206032A (en) | 1991-05-22 | 1994-12-27 | Mycogen Corp | New isolates of bacillus thuringiensis active against pests of hymenopterans and genes encoding active toxins for hymenopterans |
WO1993001283A1 (en) | 1991-07-08 | 1993-01-21 | The United States Of America As Represented By The Secretary Of Agriculture | Selection-gene-free transgenic plants |
WO1993018643A1 (en) | 1992-03-24 | 1993-09-30 | Rice Breeding Research Laboratories | Process for reducing seed storage proteins and process for transforming plants |
US5591616A (en) | 1992-07-07 | 1997-01-07 | Japan Tobacco, Inc. | Method for transforming monocotyledons |
JPH06276872A (en) | 1993-03-31 | 1994-10-04 | Mitsubishi Corp | How to remove marker gene |
-
1995
- 1995-10-25 JP JP31343295A patent/JP3256952B2/en not_active Expired - Fee Related
- 1995-11-07 AR AR33413395A patent/AR000479A1/en unknown
- 1995-11-08 CA CA002162449A patent/CA2162449C/en not_active Expired - Fee Related
- 1995-11-08 PL PL95320201A patent/PL184707B1/en not_active IP Right Cessation
- 1995-11-08 SK SK569-97A patent/SK56997A3/en unknown
- 1995-11-08 WO PCT/JP1995/002283 patent/WO1996015252A2/en not_active Application Discontinuation
- 1995-11-08 DE DE69534525T patent/DE69534525D1/en not_active Expired - Fee Related
- 1995-11-08 AU AU38557/95A patent/AU703485B2/en not_active Ceased
- 1995-11-08 NZ NZ295256A patent/NZ295256A/en unknown
- 1995-11-08 UA UA97062658A patent/UA40661C2/en unknown
- 1995-11-08 BR BRPI9509715-5A patent/BR9509715B1/en not_active IP Right Cessation
- 1995-11-08 AT AT95117589T patent/ATE307211T1/en not_active IP Right Cessation
- 1995-11-08 ES ES95117589T patent/ES2248795T3/en not_active Expired - Lifetime
- 1995-11-08 CZ CZ971388A patent/CZ138897A3/en unknown
- 1995-11-08 DK DK95117589T patent/DK0716147T3/en active
- 1995-11-08 AP APAP/P/1997/000980A patent/AP9700980A0/en unknown
- 1995-11-08 EP EP05006037A patent/EP1602729A3/en not_active Withdrawn
- 1995-11-08 EP EP95117589A patent/EP0716147B1/en not_active Expired - Lifetime
- 1995-11-08 HU HU9702174A patent/HUT77074A/en unknown
- 1995-11-09 US US08/555,760 patent/US5965791A/en not_active Expired - Lifetime
- 1995-11-09 KR KR1019950040910A patent/KR100343759B1/en not_active Expired - Fee Related
- 1995-11-09 CN CN95120511A patent/CN1073624C/en not_active Expired - Fee Related
-
1997
- 1997-05-07 NO NO972108A patent/NO972108L/en unknown
- 1997-05-07 FI FI971961A patent/FI971961L/en not_active IP Right Cessation
- 1997-05-09 OA OA70004A patent/OA10607A/en unknown
- 1997-05-29 BG BG101524A patent/BG62892B1/en unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0334383A2 (en) * | 1988-03-25 | 1989-09-27 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Transgenic plant with modified physiology, morphology and modified hormone metabolism, tissue cultures of this plant and process for its preparation |
WO1992001370A1 (en) * | 1990-07-19 | 1992-02-06 | The Regents Of The University Of California | Biologically safe plant transformation system |
WO1994017176A1 (en) * | 1993-01-29 | 1994-08-04 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
Non-Patent Citations (1)
Title |
---|
A. SPENA ET AL.: "Cell-autonomous behavior of the rolC gene of Agrobacterium rhizogenes during leaf development: a visual assay for transposon excision in transgenic plants", PLANT CELL, vol. 1, 1989, pages 1157 - 1164, XP002002909 * |
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