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WO1996013203A1 - Procede non invasif de mesure d'analytes dans le sang - Google Patents

Procede non invasif de mesure d'analytes dans le sang Download PDF

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Publication number
WO1996013203A1
WO1996013203A1 PCT/US1994/012417 US9412417W WO9613203A1 WO 1996013203 A1 WO1996013203 A1 WO 1996013203A1 US 9412417 W US9412417 W US 9412417W WO 9613203 A1 WO9613203 A1 WO 9613203A1
Authority
WO
WIPO (PCT)
Prior art keywords
body part
blood
volume ratio
tissue volume
invasively
Prior art date
Application number
PCT/US1994/012417
Other languages
English (en)
Inventor
Richard L. Wiggins
Original Assignee
Diasense, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU61865/94A priority Critical patent/AU6186594A/en
Priority to EP94107137A priority patent/EP0623308A1/fr
Priority to CA002123151A priority patent/CA2123151A1/fr
Priority to JP6120684A priority patent/JPH07136151A/ja
Application filed by Diasense, Inc. filed Critical Diasense, Inc.
Priority to PCT/US1994/012417 priority patent/WO1996013203A1/fr
Publication of WO1996013203A1 publication Critical patent/WO1996013203A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/1455—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue using optical sensors, e.g. spectral photometrical oximeters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue
    • A61B5/14532—Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value ; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid or cerebral tissue for measuring glucose, e.g. by tissue impedance measurement

Definitions

  • This invention relates to techniques for non- invasively detecting the concentration of analytes in the blood of living animals, and in particular to the use of infrared and near-infrared spectroscopic techniques for the non-invasive detection of glucose concentrations in the blood of humans.
  • the concentration of various constituents in the blood In the diagnosis and treatment of various conditions, it is important to measure the concentration of various constituents in the blood. For example, in the treatment of diabetes, the concentration of glucose in the blood must be measured on a periodic basis. For persons experiencing insulin-dependent or Type I diabe ⁇ tes, it is often necessary or desirable to measure blood glucose concentration several times each day. Obtaining accurate readings of cholesterol concentrations is impor ⁇ tant in the prevention of coronary artery disease. The measurement of the concentration of other blood analytes such as bilirubin and alcohol, is also important for various diagnostic purposes.
  • non-invasive blood glucose detection techniques have been proposed.
  • One of the most promising of these techniques is the non-invasive infrared spectroscopic technique.
  • a portion of the patient's skin is irradiated with infrared or near-infrared radia ⁇ tion, either at selected wavelengths or over a portion of the spectrum. Radiation that is either back-scattered or transmitted through a body part such as the finger, is then measured.
  • spectroscopic analysis techniques it has been hoped that the concentration of glucose in the blood can be determined.
  • a method for the detection of the concentration of an analyte in blood of a living animal comprises the steps of non-invasively irradiating a body part of the animal having a first blood volume to tissue volume ratio, detecting radiation emitted from the body part, non-invasively irradiating a body part having a second blood volume to tissue volume ratio, detecting radiation emitted from the body part, and calculating the concen ⁇ tration of the analyte in blood based on the detected radiation.
  • a method for calibrating an instrument for non- invasively determining the concentration of an analyte in the blood of a living animal comprises the steps of non-
  • ⁇ UBS ⁇ TUTE SHEET (RULE 2$) invasively irradiating a body part of the animal having a first blood volume to tissue volume ratio and detecting radiation emitted from the body part to obtain a first absorbance spectrum, non-invasively irradiating a body part of the animal having a second blood volume to tissue volume ratio, and detecting the radiation emitted by the body part, to obtain a second absorbance spectrum, invas ⁇ ively detecting the concentration of the analyte in the blood of the animal substantially simultaneously with said steps of irradiating to obtain the first and second absorbance spectra, and employing the first absorbance spectrum, the second absorbance spectrum, and the invasi- vely-detected concentration, to calibrate the instrument.
  • An apparatus for the non-invasive detection of the concentration of a constituent of blood of a living animal includes means for non-invasively irradiating a body part of the animal, means for detecting radiation emitted from the body part, and data processing means for receiving a first signal from the detecting means repre ⁇ sentative of the intensity of radiation detected during irradiation of a body part having a first blood volume to tissue volume ratio and calculating and storing a first absorbance spectrum based on the first signal; receiving a second signal from the detecting means representative of the intensity of radiation detected during irradiation of a body part having a second blood volume to tissue volume ratio and calculating and storing a second absor ⁇ saye spectrum based on the second signal; and calculat ⁇ ing from the first absorbance spectrum and the second absorbance spectrum the concentration of the constituent in the blood.
  • An apparatus for calibrating an instrument for non-invasive determination of the concentration of an analyte in the blood of a living animal includes means for non-invasively irradiating a body part of the animal; means for detecting radiation emitted from the body part; and data processing means for receiving a first signal from the detecting means representative of the intensity of radiation detected during irradiation of a body part having a first blood volume to tissue volume ratio and calculating and storing a first absorbance spectrum based on the first signal, receiving a second signal from the detecting means representative of the intensity of radia ⁇ tion detected during irradiation of a body part having a second blood volume to tissue volume ratio and calculat ⁇ ing and storing a second absorbance spectrum based on the second signal; receiving an input representing an invasi- vely-detected concentration of the analyte in the blood of the animal; and based on the first absorbance spec ⁇ trum, the second absorbance spectrum and the invasively- detected concentration, calibrating the instrument
  • Figure 1 is a sectional, schematic representa ⁇ tion of a step in a method of non-invasive detection of analyte concentration in the blood.
  • Figure 2 is a schematic representation of a method of non-invasive detection of analyte concentration in the blood stream.
  • Figure 3 is a sectional, schematic representa ⁇ tion a step in non-invasive detection of analyte concen ⁇ tration in the blood.
  • Figure 4 is a flow diagram illustrating the steps in a method for the calibration of an apparatus for the non-invasive detection of analyte concentration in the blood.
  • Figure 5 is a flow diagram illustrating the steps in a method for the non-invasive detection of analyte concentration in the blood.
  • Figures 6A and 6B illustrate the step of irra ⁇ diating an earlobe at two different blood volume to tissue volume ratios in a method according to the inven ⁇ tion
  • Figures 6C and 6D illustrate the step of irra ⁇ diating an earlobe and detecting radiation transmitted through the earlobe at two different blood volume to tissue volume ratios in a method according to the inven ⁇ tion.
  • Figure 7A and 7B illustrate the step of irradi ⁇ ating a finger tip at two different blood volume to tissue volume ratios in a method of the invention.
  • Figures 8A and 8B illustrate the step of irra ⁇ diating the skin over a vein at two different blood volume to tissue volume ratios in a method of the inven ⁇ tion.
  • Figure 9 is a block diagram illustrating an apparatus for obtaining two different spectra showing absorbance at a first blood volume to tissue volume ratio and at a second blood volume to tissue volume ratio in a method of the invention.
  • Figure 10A is a schematic representation of an apparatus for obtaining a first absorption spectrum at a first blood volume to tissue volume ration and a second absorption spectrum at a second blood volume to tissue volume ratio in a method of the invention.
  • Figure 10B is a flow diagram illustrating the steps in a method of calibrating an apparatus for the non-invasive detection of blood analyte concentrations according to the invention.
  • Figure IOC is a flow diagram illustrating the steps in a method of obtaining a blood analyte concentration reading in accordance with the invention.
  • Figures 11A and 11B illustrate the step of irradiating an inner surface of the mouth at two differ ⁇ ent blood volume to tissue volume ratios in a method in accordance with the invention.
  • Incident infrared radia ⁇ tion is transmitted by incident optical fiber 10 into skin 30 of the individual. Back-scattered infrared radiation from skin 30 is received in pickup optical fiber 20 and transmitted to glucose concentration sensor 70.
  • Incident optical fiber 10 and pick-up optical fiber 20 may each be a bundle of optical fibers.
  • Optical fiber bundle 10 and optical fiber bundle 20 may be in any desired geometric relationship.
  • radia ⁇ tion travels through a dead cell layer 34, a layer of live cells, or basal layer, 38, before entering the capillary region, and specifically papillae 42.
  • the papillae each contain a large numbers of capillaries 46 and live cells. If radiation penetrates still deeper in skin 30 before being back-scattered, it will enter the portion of the dermis containing the venules 52 and arterioles of the superficial plexus 50.
  • schemati ⁇ cally the transmission of radiation through blood and tissue. Radiation is transmitted from an emitter (not shown) through first optical fiber 210 into skin 230. Radiation from optical fiber 210 passes through a length b ⁇ of tissue 232 and a length b B of blood 234 before being received in pickup optical fiber 220 and trans ⁇ mitted to a detector (not shown) . According to Beers' Law, the intensity of transmitted or transflected radia ⁇ tion I ⁇ , is given by:
  • I 0 is the intensity of the incident radiation
  • A- is the absorbance.
  • the absorbance A- of radiation by glucose in the example shown schematically in Figure 2 may be repre ⁇ sented as
  • C GB is the concentration of glucose in blood
  • C GT is the concentration of glucose in tissue
  • a G is a constant characteristic of the absorbance of light by glucose.
  • the concentration of glucose in the bloodstream and in the cells is not identical.
  • concentration of glucose in the blood is not identical.
  • a body part of an animal having a first blood volume to tissue volume ratio is irradiated, and reflect ⁇ ed or absorbed radiation emitted from the body part is detected, as is conventional.
  • this step is illustrated in box 100 as "IRRADIATE AND DETECT AT FIRST BLOOD VOLUME TO TISSUE VOLUME RATIO.”
  • This step may be accomplished using, as shown in Figure l, conventional techniques.
  • Infrared radiation source 5 may be a tungsten filament bulb, maintained thermally isolated, with a constant current through the filament and an infrared filter intermediate the bulb and optical fiber bundle 10.
  • Radiation source 5 preferably provides radiation in a continuous spectrum between about lOOOnm and about 2500nm.
  • Pick-up optical fiber (or bundle of optical fibers) 20 transmits radiation emitted from the body part 30 to spectrometer 80.
  • spectrometer 80 may be, for example, a unitary block of appropriate glass in a Czerny-Turner configuration.
  • Detector 85 may be, as is conventional in infrared and near-infrared detection, an array of lead- sulfide detectors.
  • a selected portion of the spectrum is focused by the spectrometer 80 on detector 85.
  • detector 85 may include 64 detectors, each covering a wavelength range of about 15nm. Electrical signals from detector 85 are transmitted to data proces ⁇ sor 90 which, in accordance with conventional data pro ⁇ cessing techniques, obtains a first absorbance spectrum, showing absorbance plotted against wavelength and stores that first absorbance spectrum.
  • a pre-amplifier, an amplifier and an analog-to-digital converter may be provided intermediate the detector array and data proces ⁇ sor 90.
  • a chopper is also preferably provided in the path of reflected light to modulate the radiation and, in combination with a lock-in amplifier, reduce the effects of noise.
  • the next step, indicated at block 105, is "IRRADIATE AND DETECT AT SECOND BLOOD VOLUME TO TISSUE VOLUME RATIO.”
  • Carrying out of this step is illustrated in Figure 3. It will be seen, referring to Figure 3, that a mass 15, disposed about optical fibers 10 and 20, is compressing skin 30. Compression of skin 30 does not affect substantially the volume of dead cells 34, cells in basal layer 38, or living cells in papillae 42 or deeper layers of the dermis. However, it will be observed that capillaries 46 in the papillae are significantly compressed. As a result, the ratio of blood volume to tissue volume is decreased. Thus a second blood volume to tissue volume ratio is obtained.
  • the next step, as shown in Figure 4, at block 110, is to "INVASIVELY DETERMINE ANALYTE CONCENTRATION IN BLOOD.”
  • This step is performed, in accordance with conventional techniques, by lancing a body part, such as a finger, to obtain a small quantity of blood, and then analyzing the blood in a high accuracy instrument.
  • a body part such as a finger
  • an analyzer manufactured by Yellow Spring Instruments may be employed.
  • the next step is the calibration of the instru ⁇ ment, as shown by block 115 in Figure 4, labeled "CALI ⁇ BRATE INSTRUMENT.”
  • the calibration step is carried out, preferably using multi-variate analytical techniques, using as data inputs the absorbance spectrum obtained at the first blood volume to tissue volume ratio, the absor ⁇ saye spectrum obtained at the second blood volume to tissue volume ratio and the analyte concentration deter ⁇ mined from analysis of the invasively-obtained blood sample.
  • the multivariate analytical technique is the method of partial least squares.
  • Various commercial software packages are avail ⁇ able that will perform the computations required for partial least squares analysis.
  • Such software packages include, for example, NSAS, by NIR Systems of Silver Spring, Maryland, and Spectra Calc, Lab-Calc and GRAMS, by Galactor Industries, of Salem, New Hampshire.
  • NSAS by NIR Systems of Silver Spring, Maryland
  • Spectra Calc, Lab-Calc and GRAMS by Galactor Industries, of Salem, New Hampshire.
  • the set of factors will, when multiplied by a given spectrum, provide the concentration of the desired analyte in the blood.
  • the first step is to "IRRADIATE AND DETECT AT FIRST BLOOD VOLUME TO TISSUE VOLUME RATIO.” This process is dis ⁇ cussed above and illustrated in Figure 1.
  • Data processor 90 stores an absorbance spectrum for the first blood volume to tissue volume ratio.
  • the next step is to "IRRADIATE AND DETECT AT SECOND BLOOD VOLUME TO TISSUE VOLUME RATIO.” This step may be accomplished while compressing the skin, as illustrated in Figure 3, or by one of the techniques explained below in connection with Figures 6-11.
  • the emitted radiation is then received by detector 85, as illustrated in Figure 1.
  • Detector 85 provides an output electrical signal representing the spectrum to data processor 90, which calculates and stores a absorption spectrum for the second blood volume to tissue volume ratio.
  • Data processor 90 will then, in accordance with conventional techniques, calculate the concentration of the analyte in blood, using a set of factors calculated during calibration of the instrument, as discussed above in connection with Figure 4.
  • This concentration is then preferably displayed on a suitable display.
  • the reading may also be stored in an appropriate memory device.
  • This step of calculation and display is indicated in Figure 5, at block 130, labeled "CALCULATE AND DISPLAY ANALYTE CONCENTRATION IN BLOOD.”
  • FIG. 6A and 6B there is illustrated a technique for obtaining readings from the ear lobe of a human patient.
  • Input optical fiber (which may also be a bundle of opti ⁇ cal fibers) 610 is in contact with the skin of ear lobe 630.
  • Pickup optical fiber (which may also be a bundle of optical fibers) 620 is also in contact with the skin of ear lobe 630.
  • an initial reading at a first blood volume to tissue volume ratio, is obtained with no pressure on the earlobe.
  • an appropriate instrument (not shown) obtains an absorbance spectrum for this reading.
  • ring 625 and mass 635 compress ear lobe 630.
  • the quantity of blood in ear lobe 630 will be substantially less than the blood volume when ear lobe 630 is not compressed as in Figure 6A.
  • a second blood volume to tissue volume ratio is obtained.
  • a reading is then taken at this second blood volume to tissue volume ratio, and an absorbance spectrum determined.
  • Input optical fiber (which may also be a bundle of optical fibers) 640, is in contact with the skin of earlobe 630, at one side thereof.
  • Pickup optical fiber (which may also be a bundle of optical fibers) 650 is also in contact with the skin of earlobe 630, but opposite to input optical fiber 640.
  • input optical fiber 640 a rigid block 645.
  • pickup optical fiber 650 a rigid block 655.
  • an appropriate instrument obtains an absorbance spectrum for this reading.
  • earlobe 630 is compressed between masses 645 and 655.
  • the quantity of blood in earlobe 630 will be substantially less than the blood volume when earlobe 630 is not compressed as in Figure 6C.
  • a reading is then taken at this second blood volume to tissue volume ratio, and an absorbance spectrum determined.
  • FIG. 7A there will be explained a method of non-invasively calibrating an instrument, and detecting a blood analyte concentration, by taking readings at a patient's finger.
  • FIG. 7A there is shown a person's finger tip 730 with incident optical fiber or optical fiber bundle 710, and pick up optical fiber or optical fiber bundle 720, in contact with the finger and adjacent to one another.
  • finger tip 730 There is in finger tip 730 a first blood volume to tissue volume ratio.
  • Incident optical fiber 710 transmits infrared radiation into finger tip 730.
  • Radiation emit ⁇ ted from finger 730 into pick up optical fiber 720 is transmitted to an analysis instrument, which obtains an absorbance spectrum for a first blood volume to tissue volume ratio.
  • finger tip 730 has been compressed between ring 735 and surface 740. Compression of finger tip 730 results in a lower, second, blood volume to tissue volume ratio. Radiation transmit ⁇ ted through incident optical fiber 710 enters finger tip 730. A portion of that radiation is emitted into pickup optical fiber 720 and transmitted to an analysis instru ⁇ ment, which calculates an absorbance spectrum for the second blood volume to tissue volume ratio. If the technique of Figures 7A and 7B is employed in calibrating an analysis instrument, a blood sample will be obtained, and concentration of the selected analyte measured by conventional analytical techniques. Using the data from the two absorbance spectra and the invasive measurement, the instrument will be calibrated.
  • FIGs 8A and 8B there is illus ⁇ trated a method according to the invention employing readings of large veins close to the skin surface.
  • a body part such as the hand 830 of an individual, with a prominent vein 832 located close to the skin.
  • An incident optical fiber or optical fiber bundle 810 transmits radiation from an emitter (not shown) to the body part 830.
  • Radiation emitted from body part 830 into pickup optical fiber or pick up optical fiber bundle 820 is transmitted to a conventional instru ⁇ ment for obtaining of an absorbance spectrum.
  • a reading is taken at a first blood volume to tissue volume ratio, as illustrated in Figure 8A, to obtain a first absorbance spectrum. Because the large vein is close to the sur ⁇ face, the blood volume to tissue volume ratio is rela ⁇ tively high.
  • a reading is taken at a second, lower, blood volume to tissue volume ratio.
  • finger 834 has been used to drain vein 832 of blood. This is done simply by pressing the finger on the vein, and running the finger outward from the heart while maintaining pressure on the vein.
  • Near-infrared radiation source 905 emits radiation in the near-infrared into an incident- optical fiber or incident optical fiber bundle 910.
  • Incident optical fiber bundle 910 splits into first branch inci ⁇ dent optical fiber bundle 912 and second branch incident optical fiber bundle 914.
  • First branch incident optical fiber bundle 912 is disposed with its end in contact with a blood rich portion of skin 930 of a patient. Blood rich portion of the skin 930 may be, for example, the inside of the wrist.
  • Blood poor portion 932 may be, for example, the upper interior forearm.
  • Infrared radiation emitted from blood rich portion of skin 930 enters pickup optical fiber or pickup optical fiber bundle 922 and is transmitted through optical fiber bundle 922 to optical switch 928. Infrared radiation from blood poor portion of skin 932 is received
  • Optical switch 928 selectively allows radiation from either pickup optical fiber bundle 922 or pickup optical bundle 924 to enter spectrometer 940.
  • Spectrometer 940 spectrally separates radiation received from pickup optical fiber bundle 922 or 924, and focuses radiation on optical detector 945.
  • Optical detector 945 provides an electrical signal representative of detected radiation to data processor 955.
  • Controller 950 controls the operation of optical switch 928, and provides signals to data processor 955, so that data processor 955 can accurately distinguish the spectra from blood rich portion of skin 930 and blood poor portion of skin 932.
  • Data processor 955 calculates an absorbance spectrum for blood rich portion of skin 930 and blood poor portion of skin 932. These absorbance spectra are processed, as explained above, either to calibrate an instrument or to calculate analyte concentration in the blood.
  • Near infrared emitter 1005 emits radiation in the near infrared. This near infrared radiation is transmitted into incident optical fiber bundle 1010. An end of incident optical fiber bundle 1010 is placed in contact with the skin of body part 1030, so that infrared radia- tion irradiates body part 1030. Radiation emitted from body part 1030 is received in pickup optical fiber or optical fiber bundle 1020 and is transmitted to spectrom ⁇ eter 1040. Radiation from body part 1030 is then spec ⁇ trally separated and focused on detector array 1045. Detector 1045 provides an electrical signal to data processor 1050 indicative of the light intensity. Data processor 1050 calculates an absorption spectrum.
  • pulse detector 1035 is physi ⁇ cally coupled to the skin of body part 1030.
  • Pulse detector 1035 is electrically coupled to, and provides an electrical signal to, data processor 1050, which signal indicates the pulse cycle in body part 1030.
  • Pulse detector 1035 may be, for example, a pressure sensor or an optical sensor that detects hemoglobin variance.
  • pulse detector 1035 provides information regard ⁇ ing the relative quantity of blood in blood vessels in body part 1030. During the cycle of the pulse in any body part, the blood volume varies and thus the blood volume to tissue volume ratio varies.
  • FIG. 10B there will be explained a method for calibrating an instrument using the device of Figure 10A in a method according to the invention.
  • the first step illustrated at block 1060, labeled "IRRADIATE AND DETECT AT SYSTOLIC," the step of irradiating body part 1030 during the systolic portion of the pulse cycle, as detected by pulse detector 1035, is indicated.
  • body part 1030 is irradiat ⁇ ed during the diastolic portion of the pulse cycle, as detected by pulse detector 1035.
  • This step is illustrat ⁇ ed by clock 1062, labeled "IRRADIATE AND DETECT AT DIA- STOLIC.”
  • Data processor 1050 has then calculated and stored absorbance spectra obtained at the systolic and diastolic portions of the pulse cycle.
  • the absorbance spectrum obtained at the systolic portion of the cycle represents a relatively high blood volume to tissue volume ratio.
  • the absorbance obtained in the diastolic portion of the cycle represents an absorbance spectrum obtained at a relatively low blood volume to tissue volume ratio.
  • the next step in the method of calibration according to Figure 10B is to obtain a blood sample and to detect the concentration of the blood analyte of interest from this sample using conventional techniques, as explained in greater detail above.
  • This step is illustrated at block 1064, labeled "INVASIVELY DETECT BLOOD ANALYTE CONCENTRATION.”
  • the next step is to calibrate the instrument, preferably in accordance with multivariate techniques, such as partial least squares.
  • This step is illustrated at block 1066, labeled "CALI ⁇ BRATE INSTRUMENT. "
  • the first step is to irradiate body part 1030 and detect the emitted radiation during the systolic portion of the pulse cycle.
  • the portion of the pulse cycle is, as explained above, detected by pulse detector 1035.
  • This first step is illustrated at block 1070, labeled "IRRADIATE AND DETECT AT SYSTOLIC.”
  • the next step is to irradiate body part 1030 at the diastolic portion of the pulse cycle.
  • the portion of the pulse cycle is detected by pulse detector 1035, which provides an appropriate electrical signal to data processor 1050.
  • This step is illustrated by block 1072, labeled "IRRADI ⁇ ATE AND DETECT AT DIASTOLIC.”
  • Data processor 1050 has now obtained and calculated absorbance spectra for a first blood volume to tissue volume ratio and a second blood volume to tissue volume ratio. As calibration has already been performed, the blood analyte concentration may now be calculated. This step of calculation is illustrated at block 1074 labeled “CALCULATE BLOOD ANALY ⁇ TE CONCENTRATION. "
  • near infra ⁇ red emitter 1005 may, through incident optical fiber bundle 1010, irradiate body part 1030 continuously over several pulse cycles.
  • the signal produced by pulse detector 1035 will then be used by data processor 1050 to characterize data received from detector 1045 as to the portion of the pulse cycle to which such data relates.
  • readings may be taken initially at any portion of the cycle, and not merely at the systolic portion of the cycle, as illus ⁇ trated in Figures 10B and IOC.
  • FIG. 11A and 11B there is illustrated a technique for obtaining readings from the interior of the mouth of a human patient.
  • the technique illustrated in Figures 11A and 11B is performed on the interior of the lip, and in particular, the interior of the lower lip, of the patient. The same technique may be applied to other interior surfaces of the mouth.
  • Input optical fiber (which may also be a bundle of optical fibers) 1110 is in contact with the interior surface of lower lip 1130.
  • Pickup optical fiber (which may also be a bundle of optical fibers) 1120 is also in contact with the interior surface of lower lip 1130.
  • a mass 1135 there is provided on the outer surface of lip 1130, opposite to rigid ring 1125, a mass 1135.
  • an initial reading at a first blood volume to tissue volume ratio, is obtained with no pressure on the lip.
  • an appropriate instrument (not shown) obtains an absorbance spectrum for this reading.
  • ring 1125 and mass 1135 compress lip 1130.
  • the quantity of blood in lip 1130 intermediate rigid ring 1125 and mass 1135 will be substantially less than the blood volume in that portion of the lip when lip 1130 is not compressed as in Figure 11A.
  • a second blood volume to tissue volume ratio is obtained. A reading is taken at this second blood volume to tissue volume ratio, and an absorbance spectrum determined.

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Abstract

La présente invention concerne un procédé de détection de la concentration d'un analyte dans le sang d'un animal vivant. Le procédé consiste à irradier une partie du corps de l'animal caractérisé par un premier rapport entre le volume sanguin et le volume tissulaire. Le procédé consiste ensuite à détecter le rayonnement émis par la partie du corps (120). Le procédé consiste ensuite à irradier de façon non invasive une partie du corps caractérisée par un second rapport entre le volume sanguin et le volume tissulaire et à détecter le rayonnement émis par la partie du corps (125), puis à calculer la concentration de l'analyte contenu dans le sang à partir du rayonnement détecté émis (130).
PCT/US1994/012417 1993-05-07 1994-10-28 Procede non invasif de mesure d'analytes dans le sang WO1996013203A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU61865/94A AU6186594A (en) 1993-05-07 1994-05-04 Method for non-invasive measurement of concentration of analytes in blood
EP94107137A EP0623308A1 (fr) 1993-05-07 1994-05-06 Mesure non-invasive de la concentration de constituants du sang
CA002123151A CA2123151A1 (fr) 1993-05-07 1994-05-09 Methode de mesure non invasive de la concentration sanguine d'analytes
JP6120684A JPH07136151A (ja) 1993-05-07 1994-05-09 血液成分の濃度を検出する方法及び装置並びにこの装置を較正するための装置
PCT/US1994/012417 WO1996013203A1 (fr) 1993-05-07 1994-10-28 Procede non invasif de mesure d'analytes dans le sang

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US5916193A 1993-05-07 1993-05-07
PCT/US1994/012417 WO1996013203A1 (fr) 1993-05-07 1994-10-28 Procede non invasif de mesure d'analytes dans le sang

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1732435A2 (fr) * 2004-04-07 2006-12-20 Sensys Medical, Inc. Appareil compact de mesure non-invasive de glucose par spectroscopie proche infrarouge

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5372135A (en) * 1991-12-31 1994-12-13 Vivascan Corporation Blood constituent determination based on differential spectral analysis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5372135A (en) * 1991-12-31 1994-12-13 Vivascan Corporation Blood constituent determination based on differential spectral analysis

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1732435A2 (fr) * 2004-04-07 2006-12-20 Sensys Medical, Inc. Appareil compact de mesure non-invasive de glucose par spectroscopie proche infrarouge
EP1732435A4 (fr) * 2004-04-07 2009-07-29 Sensys Medical Inc Appareil compact de mesure non-invasive de glucose par spectroscopie proche infrarouge

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