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WO1996012824A3 - Emploi de spermidine pour attenuer les effets inhibiteurs de reactions en chaine de ligase dans un echantillon d'essai clinique - Google Patents

Emploi de spermidine pour attenuer les effets inhibiteurs de reactions en chaine de ligase dans un echantillon d'essai clinique

Info

Publication number
WO1996012824A3
WO1996012824A3 PCT/US1995/012874 US9512874W WO9612824A3 WO 1996012824 A3 WO1996012824 A3 WO 1996012824A3 US 9512874 W US9512874 W US 9512874W WO 9612824 A3 WO9612824 A3 WO 9612824A3
Authority
WO
WIPO (PCT)
Prior art keywords
spermidine
chain reaction
ligase chain
test sample
clinical test
Prior art date
Application number
PCT/US1995/012874
Other languages
English (en)
Other versions
WO1996012824A2 (fr
Inventor
Alan H Davis
Jianli Cao
Eui H Lee
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Priority to EP95938175A priority Critical patent/EP0791075A2/fr
Priority to JP51396296A priority patent/JP2002501361A/ja
Publication of WO1996012824A2 publication Critical patent/WO1996012824A2/fr
Publication of WO1996012824A3 publication Critical patent/WO1996012824A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé amélioré d'amplification d'acides nucléiques cibles et en particulier procédé d'atténuation des effets inhibiteurs de certains échantillons lors de réactions d'amplification en chaine par ligases. L'atténuation de l'inhibition s'obtient par adjonction au mélange d'amplification d'une quantité efficace d'un réactif de type polyamine tel que la spermidine, par exemple de 1 à 4 mM. Dans des variantes, la présence de la spermidine permet l'amplification en présence de concentrations réduites de MgCl2 et de ligase.
PCT/US1995/012874 1994-10-21 1995-10-18 Emploi de spermidine pour attenuer les effets inhibiteurs de reactions en chaine de ligase dans un echantillon d'essai clinique WO1996012824A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP95938175A EP0791075A2 (fr) 1994-10-21 1995-10-18 Emploi de spermidine pour attenuer les effets inhibiteurs de reactions en chaine de ligase dans un echantillon d'essai clinique
JP51396296A JP2002501361A (ja) 1994-10-21 1995-10-18 臨床検査試料におけるリガーゼ連鎖反応の阻害を軽減するためのスペルミジンの使用

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US33139194A 1994-10-21 1994-10-21
US08/331,391 1994-10-21
US53221295A 1995-10-12 1995-10-12
US08/532,212 1995-10-12

Publications (2)

Publication Number Publication Date
WO1996012824A2 WO1996012824A2 (fr) 1996-05-02
WO1996012824A3 true WO1996012824A3 (fr) 1996-06-27

Family

ID=26987744

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/012874 WO1996012824A2 (fr) 1994-10-21 1995-10-18 Emploi de spermidine pour attenuer les effets inhibiteurs de reactions en chaine de ligase dans un echantillon d'essai clinique

Country Status (3)

Country Link
EP (1) EP0791075A2 (fr)
CA (1) CA2202990A1 (fr)
WO (1) WO1996012824A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136533A (en) * 1997-07-03 2000-10-24 Id Biomedical Additives for use in cycling probe reactions
AU4754300A (en) * 1999-04-30 2000-12-12 Connex Gesellschaft Zur Optimierung Von Forschung Und Entwicklung Mbh Mixture for neutralizing enzyme inhibitors
US20150218608A1 (en) * 2014-01-31 2015-08-06 New England Biolabs, Inc. Compositions and Methods for Improvement of Ligation Yields
EP3438258B1 (fr) 2016-03-30 2021-09-22 FUJIFILM Wako Pure Chemical Corporation Jeu d'amorce pour la détection de chlamydia trachomatis, méthode de détection de chlamydia trachomatis utilisant ce dernier, et kit de réactifs associé

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6236187A (ja) * 1985-08-09 1987-02-17 Takara Shuzo Co Ltd Ｄｎａリガ−ゼ組成物及びその使用方法
EP0320308A2 (fr) * 1987-12-11 1989-06-14 Abbott Laboratories Procédé pour détecter une séquence cible d'acide nucléique
WO1991017239A1 (fr) * 1990-05-03 1991-11-14 Cornell Research Foundation, Inc. Systeme d'amplification d'adn induit par ligase thermostable, servant a detecter des maladies genetiques.
JPH06277061A (ja) * 1993-03-30 1994-10-04 Shimadzu Corp 核酸合成法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6236187A (ja) * 1985-08-09 1987-02-17 Takara Shuzo Co Ltd Ｄｎａリガ−ゼ組成物及びその使用方法
EP0320308A2 (fr) * 1987-12-11 1989-06-14 Abbott Laboratories Procédé pour détecter une séquence cible d'acide nucléique
WO1991017239A1 (fr) * 1990-05-03 1991-11-14 Cornell Research Foundation, Inc. Systeme d'amplification d'adn induit par ligase thermostable, servant a detecter des maladies genetiques.
JPH06277061A (ja) * 1993-03-30 1994-10-04 Shimadzu Corp 核酸合成法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 8712, Derwent World Patents Index; AN 87-084125, XP002001117, "dna synthetase composition option temperature degree contain dna synthetase polyethylene glycol one mono di valence cation poly amine" *
DATABASE WPI Week 9444, Derwent World Patents Index; AN 94-353745, XP002001116, "nucleic acid amplify presence poly amine efficiency remove inhibit cause impure biological sample" *

Also Published As

Publication number Publication date
WO1996012824A2 (fr) 1996-05-02
CA2202990A1 (fr) 1996-05-02
EP0791075A2 (fr) 1997-08-27

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