+

WO1996007755A3 - Method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria - Google Patents

Method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria Download PDF

Info

Publication number
WO1996007755A3
WO1996007755A3 PCT/CZ1995/000020 CZ9500020W WO9607755A3 WO 1996007755 A3 WO1996007755 A3 WO 1996007755A3 CZ 9500020 W CZ9500020 W CZ 9500020W WO 9607755 A3 WO9607755 A3 WO 9607755A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorochrome
indicators
substrates
pathogenic bacteria
potentially pathogenic
Prior art date
Application number
PCT/CZ1995/000020
Other languages
French (fr)
Other versions
WO1996007755A2 (en
Inventor
Bozena Sedlinska
Jin Haeusler
Vlastimil Stetina
Original Assignee
Lachema As
Bozena Sedlinska
Jin Haeusler
Vlastimil Stetina
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lachema As, Bozena Sedlinska, Jin Haeusler, Vlastimil Stetina filed Critical Lachema As
Publication of WO1996007755A2 publication Critical patent/WO1996007755A2/en
Publication of WO1996007755A3 publication Critical patent/WO1996007755A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/20—Coumarin derivatives
    • C12Q2334/22—4-Methylumbelliferyl, i.e. beta-methylumbelliferone, 4MU

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria, based on that onto a moistened adsorption matrix saturated with a fluorochrome substrate there is transfered from a selective culture medium an incubated membrane filter with colonies of microorganisms which upon further cultivation for 1 to 4 hours at an optimal temperature for the microorganisms in question is exposed to U.V. rays of a wave length of 350 to 370 nm.
PCT/CZ1995/000020 1994-09-09 1995-09-11 Method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria WO1996007755A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CZPV2192-94 1994-09-09
CZ942192A CZ282401B6 (en) 1994-09-09 1994-09-09 Method of making conformity tests by making use of fluorochrome substrata in indicators of faecal contamination or potentially pathogenic bacteria

Publications (2)

Publication Number Publication Date
WO1996007755A2 WO1996007755A2 (en) 1996-03-14
WO1996007755A3 true WO1996007755A3 (en) 1996-07-25

Family

ID=5464527

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CZ1995/000020 WO1996007755A2 (en) 1994-09-09 1995-09-11 Method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria

Country Status (2)

Country Link
CZ (1) CZ282401B6 (en)
WO (1) WO1996007755A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3496677B1 (en) 2016-08-12 2021-03-17 Coloplast A/S An ostomy appliance

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0108303A1 (en) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Method for detection and isolation of a microorganism
WO1986001903A1 (en) * 1984-09-19 1986-03-27 Board Of Regents, The University Of Texas System Colony blot assay for enterotoxigenic bacteria
US4591554A (en) * 1979-10-31 1986-05-27 Ajinomoto Co., Inc. Rapid method for detecting microorganisms
GB2178847A (en) * 1985-08-07 1987-02-18 Philips Electronic Associated Testing for the presence of living organisms at the surface of an object
WO1990012888A1 (en) * 1989-04-27 1990-11-01 Biocontrol Systems, Incorporated Precipitate test for microorganisms

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591554A (en) * 1979-10-31 1986-05-27 Ajinomoto Co., Inc. Rapid method for detecting microorganisms
EP0108303A1 (en) * 1982-11-01 1984-05-16 Miles Laboratories, Inc. Method for detection and isolation of a microorganism
WO1986001903A1 (en) * 1984-09-19 1986-03-27 Board Of Regents, The University Of Texas System Colony blot assay for enterotoxigenic bacteria
GB2178847A (en) * 1985-08-07 1987-02-18 Philips Electronic Associated Testing for the presence of living organisms at the surface of an object
WO1990012888A1 (en) * 1989-04-27 1990-11-01 Biocontrol Systems, Incorporated Precipitate test for microorganisms

Also Published As

Publication number Publication date
WO1996007755A2 (en) 1996-03-14
CZ282401B6 (en) 1997-07-16
CZ219294A3 (en) 1996-03-13

Similar Documents

Publication Publication Date Title
Dias et al. Microbial ecology of activated sludge: I. Dominant bacteria
CA2015496A1 (en) Microbiological dry culture medium
FR2708286B1 (en) Method of identifying microorganisms with at least two chromogens.
MX9602924A (en) Method for detecting staphylococci.
AU2127888A (en) Detection methods
CA2338249A1 (en) Biodispersion as a method for removal of hydrocarbon oil from marine aqueous environments
ES2104408T3 (en) PROCEDURE OF IDENTIFICATION OF MICROORGANISMS WITH A SUPPLEMENTED MEDIA WITH CARBON HYDRATE.
IN190120B (en)
YU55601A (en) Process for the preparation of pseudominic acid a antibiotic by microbiological method
CA2182511A1 (en) Microbiological Culture Bottle, and Method of Making and Using Same
WO1996007755A3 (en) Method of performing confirmatory tests by fluorochrome substrates in indicators of fecal contamination or potentially pathogenic bacteria
Sands et al. Ecology and physiology of fluorescent pectolytic pseudomonads
ES2160829T3 (en) PROCEDURE FOR OBTAINING RIBOFLAVINE THROUGH MICROORGANISMS WITH MODIFIED ISOCITRATOLIASA ACTIVITY.
AU2159801A (en) Test for micro-organisms
ES2166544T3 (en) PROCEDURE FOR THE AEROBIC BIODEGRATION OF DIFFICULTLY HYDROSOLUBBLE SUBSTANCES AND MICROORGANISMS CEPA IHI-91.
DE69632373D1 (en) Selective media for the cultivation and isolation of Gram-negative bacteria, antibiotic composition
KR840000641A (en) Microbial Automatic Screening Method
RU2204597C2 (en) Strain of archaebacterium bacillus species vkpm b-5061 as decomposing agent petroleum products and phosphate used in soil and sewage treatment
ATE5199T1 (en) PROCESS FOR OBTAINING GLUCOSE DEHYDROGENASE AND SUITABLE MICROORGANISMS THEREOF.
CA2225485A1 (en) Microbiological degradation of alkylene amine acetates
JPS54157896A (en) Preparation of prumycin
DE3469566D1 (en) Aerobic microbiological method
SU1375646A1 (en) Bacterial strain xanthomonas sp. for purifying effluents from tetrahydrofuran
JPS5664793A (en) Preparation of l-phenylalanine
Sharma et al. Properties of a cellulolytic Sporolactobacillus and some non‐sporing cellulolytic rods, presumptive clostridia, from an anaerobic digester

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AT CA CN DE DK ES FI GB HU JP MX NO PL RU SE US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载