WO1996001417A1 - Spectrophotometric equipment and use thereof - Google Patents
Spectrophotometric equipment and use thereof Download PDFInfo
- Publication number
- WO1996001417A1 WO1996001417A1 PCT/SE1995/000808 SE9500808W WO9601417A1 WO 1996001417 A1 WO1996001417 A1 WO 1996001417A1 SE 9500808 W SE9500808 W SE 9500808W WO 9601417 A1 WO9601417 A1 WO 9601417A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cuvette
- light
- equipment according
- rear wall
- spectrophotometric equipment
- Prior art date
Links
- 239000000523 sample Substances 0.000 claims abstract description 21
- 230000003287 optical effect Effects 0.000 claims abstract description 10
- 238000005259 measurement Methods 0.000 claims abstract description 4
- 230000031700 light absorption Effects 0.000 claims abstract description 3
- 239000000463 material Substances 0.000 claims description 12
- 238000004458 analytical method Methods 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 239000002002 slurry Substances 0.000 claims description 5
- 239000008187 granular material Substances 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 239000010453 quartz Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 230000005540 biological transmission Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000012254 powdered material Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000013179 statistical model Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
Definitions
- the present invention refers to a spectrophotometric equipment comprising a light emitting source in front of a cuvette and a detector behind said cuvette.
- the detector measures the absorp ⁇ tion, in a sample material charged in the cuvette, of the light from the light source.
- the emitted light or a portion of the emitted light passes through a transparent front wall of the cuvette, the sample material and a transparent rear wall of the cuvette.
- the invention furthermore relates to the use of said spectrophotometric equipment.
- Emitted light from the light source can be a so called white light or a monochromatic light, emanating from a monochromator of suitable constructional design.
- the detector can be a simple photodetector or consist of a dispersion part, such as a poly- chromator together with a number of detectors for certain wave ⁇ lengths.
- An ordinary spectrophotometer setting is based on reflection, i.e. light reflected from an exposed sample in the cuvette of a spectrophotometer is measured.
- Spectrophotometers based on transmission are also well-known. The amount of light passing through the sample is thereby mea ⁇ sured.
- the present invention relates to such a spectrophoto ⁇ meter equipment.
- Cuvettes used with transmission spectrophotometers are designed with a front and a rear wall forming a right angle in relation to the optical axis of the light emitted from the light source.
- Cuvettes having such a design very often perform poorly with many products of interest for spectrophotometric analyses.”
- the poor performance is normally related to the chemical and physi ⁇ cal nature and properties of the products, which cause large variations in the absorbency levels between different samples as well as between different measurements on the same sample. Varying structures of various samples imply similar problems due to varying density when charged in a cuvette. The samples can thus not be satisfactorily analysed using an equipment as herein disclosed.
- the present invention refers to a spectrophotometric equipment comprising a light emitting source arranged in front of a cuvette and a detector arranged behind said cuvette, which detector measures the absorption of emitted light in a sample material introduced into the cuvette.
- the emitted light or a portion of the said light passes, prior to recording in the detector, through a transparent front wall of the cuvette, the sample material and a transparent rear wall of the cuvette.
- the equipment is characterised in that the front wall and/or the rear wall of the cuvette forms an oblique angle in relation to the optical axis of the emitted light.
- the cuvette can thereby be wedge-shaped, cylindrical, spherical or the like giving above oblique angle in relation to said optical axis.
- At least the front and rear wall of the cuvette are made of glass, quartz or other material transparent to the wavelengths of used light.
- the front wall of the cuvette is, according to one preferred embodiment of the invention, designed to give an oblique angle in relation to the optical axis of the light emitted from the light source, while the rear wall forms a right angle in rela ⁇ tion of said optical axis.
- the aforementioned embodiments of the cuvette according to the present invention give the major advantages of less variations in absorbency levels between different sample materials and types as well as a reduced difference between the highest and the lowest value of each spectra.
- a single transmission spect ⁇ rophotometer can thus with said cuvette at a very high degree of reliability analyse a large number of different materials.
- Used light source normally works within the wavelength area of 800-1100 nm, which is near the infrared light.
- the light that passes through the cuvette has a comparatively narrow divergence when entering the sample but rapidly diverges through reflection and change of direction as soon as it has entered into the sample material.
- 1/100 to 1/100000 of entered light passes through a finely powdered sample, which sample state is a preferred embodiment of the invention. It is often, in order to reproduce analytical results, necessary to prepare a sample material used in different analysis similarly or equally.
- the design of the cuvette according to the present invention furthermore, facilitates filling and packing of powdered mate ⁇ rials thereinto.
- the sample material can, of course, also be present as granules, a slurry, a paste, a liquid or the like.
- the present invention also refers to the use of aforementioned spectrophotometric equipment for analyses of materials such as fodders and foodstuffs, whereby these products are available as granules, a powder, a slurry, a paste or a liquid or are trans ⁇ ferred into any of above states prior to the analysis.
- the use is, of course, not limited to above materials and/or states.
- the equipment according to the present invention can advanta ⁇ geously be used for analysis of all types of sample materials giving rise to discussed problems.
- the equipment according to the present invention is especially suitable for analyses of materials containing fat and/or protein.
- the spectrophotometric equipment comprises a monochromatic light source 1 arranged in front of a cuvette 2 and a detector unit 2 arranged behind said cuvette 2.
- a finely powdered material 4, a fodder is charged in the cuvette 2.
- Light emitted from the light source 1 will pass through a transparent front wall 5 of the cuvette 2, the fodder 4 and a transparent rear wall 6 of the cuvette 2 prior to measurement in the detector 3.
- the front wall 5 forms an oblique angle in relation to the optical axis 7 of the light.
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Spectrophotometric equipment comprising a light source (1) arranged in front of a cuvette (2) and a detector (3) arranged behind said cuvette (2). The detector (3) measures absorption of light, emitted from the light source (1), in a sample material (4) charged in the cuvette (2), whereby the light or a portion of the light passes through a front wall (5) of the cuvette (2), the sample material (4) and a rear wall (6) of the cuvette prior to measurement in the detector (3). The equipment is characterised in that the front wall (5) and/or the rear wall (6) of the cuvette (2) forms an oblique angle in relation to the optical axis (7) of the emitted light.
Description
SPECTROPHOTOMETRIC EQUIPMENT AND USE THEREOF
The present invention refers to a spectrophotometric equipment comprising a light emitting source in front of a cuvette and a detector behind said cuvette. The detector measures the absorp¬ tion, in a sample material charged in the cuvette, of the light from the light source. The emitted light or a portion of the emitted light passes through a transparent front wall of the cuvette, the sample material and a transparent rear wall of the cuvette. The invention furthermore relates to the use of said spectrophotometric equipment.
Emitted light from the light source can be a so called white light or a monochromatic light, emanating from a monochromator of suitable constructional design. The detector can be a simple photodetector or consist of a dispersion part, such as a poly- chromator together with a number of detectors for certain wave¬ lengths.
An ordinary spectrophotometer setting is based on reflection, i.e. light reflected from an exposed sample in the cuvette of a spectrophotometer is measured.
Spectrophotometers based on transmission are also well-known. The amount of light passing through the sample is thereby mea¬ sured. The present invention relates to such a spectrophoto¬ meter equipment.
Cuvettes used with transmission spectrophotometers are designed with a front and a rear wall forming a right angle in relation to the optical axis of the light emitted from the light source. Cuvettes having such a design very often perform poorly with many products of interest for spectrophotometric analyses."The
poor performance is normally related to the chemical and physi¬ cal nature and properties of the products, which cause large variations in the absorbency levels between different samples as well as between different measurements on the same sample. Varying structures of various samples imply similar problems due to varying density when charged in a cuvette. The samples can thus not be satisfactorily analysed using an equipment as herein disclosed.
It has by means of the present invention quite surprisingly been possible to eliminate or substantially reduce above prob¬ lems. The present invention refers to a spectrophotometric equipment comprising a light emitting source arranged in front of a cuvette and a detector arranged behind said cuvette, which detector measures the absorption of emitted light in a sample material introduced into the cuvette. The emitted light or a portion of the said light passes, prior to recording in the detector, through a transparent front wall of the cuvette, the sample material and a transparent rear wall of the cuvette. The equipment is characterised in that the front wall and/or the rear wall of the cuvette forms an oblique angle in relation to the optical axis of the emitted light. The cuvette can thereby be wedge-shaped, cylindrical, spherical or the like giving above oblique angle in relation to said optical axis.
At least the front and rear wall of the cuvette are made of glass, quartz or other material transparent to the wavelengths of used light.
The front wall of the cuvette is, according to one preferred embodiment of the invention, designed to give an oblique angle in relation to the optical axis of the light emitted from the light source, while the rear wall forms a right angle in rela¬ tion of said optical axis.
The aforementioned embodiments of the cuvette according to the present invention give the major advantages of less variations in absorbency levels between different sample materials and types as well as a reduced difference between the highest and the lowest value of each spectra. A single transmission spect¬ rophotometer can thus with said cuvette at a very high degree of reliability analyse a large number of different materials.
Used light source normally works within the wavelength area of 800-1100 nm, which is near the infrared light.
The light that passes through the cuvette has a comparatively narrow divergence when entering the sample but rapidly diverges through reflection and change of direction as soon as it has entered into the sample material. Normally, 1/100 to 1/100000 of entered light passes through a finely powdered sample, which sample state is a preferred embodiment of the invention. It is often, in order to reproduce analytical results, necessary to prepare a sample material used in different analysis similarly or equally.
The design of the cuvette according to the present invention, furthermore, facilitates filling and packing of powdered mate¬ rials thereinto. The sample material can, of course, also be present as granules, a slurry, a paste, a liquid or the like.
The present invention also refers to the use of aforementioned spectrophotometric equipment for analyses of materials such as fodders and foodstuffs, whereby these products are available as granules, a powder, a slurry, a paste or a liquid or are trans¬ ferred into any of above states prior to the analysis. The use is, of course, not limited to above materials and/or states. The equipment according to the present invention can advanta¬ geously be used for analysis of all types of sample materials
giving rise to discussed problems. The equipment according to the present invention is especially suitable for analyses of materials containing fat and/or protein.
The invention and the attendant advantages will be more fully understood from the following detailed description, taken in conjunction with appended Figure 1 showing an embodiment of a spectrophotometric equipment according to the present invention. The spectrophotometric equipment comprises a monochromatic light source 1 arranged in front of a cuvette 2 and a detector unit 2 arranged behind said cuvette 2. A finely powdered material 4, a fodder, is charged in the cuvette 2. Light emitted from the light source 1 will pass through a transparent front wall 5 of the cuvette 2, the fodder 4 and a transparent rear wall 6 of the cuvette 2 prior to measurement in the detector 3. The front wall 5 forms an oblique angle in relation to the optical axis 7 of the light.
Recorded light absorption will be used for calculation of dif¬ ferent parameters by means of statistical methods and models.
All obtained analytical results are, compared to analyses using a conventional cuvette, more accurate.
While a particular embodiment of the invention has been shown, it will be understood, of course, that the invention is not limited thereto since many modifications may be made, and it is, therefore, contemplated to cover by the appended claims any such modifications as fall within the true spirit and scope of the invention.
Claims
1. Spectrophotometric equipment comprising a light source (1) arranged in front of a cuvette (2) and a detector or detec¬ tor unit (3) arranged behind said cuvette (2), which detec¬ tor (3) measures absorption of light, emitted from the light source (1) , in a sample material (4) charged in the cuvette (2), whereby the light or a portion of the light passes through a front wall (5) of the cuvette (2), the sample material (4) and a rear wall (6) of the cuvette prior to measurement in the detector (3) c h a r a c t e r i s e d i n, that the front wall (5) and/or the rear wall (6) of the cuvette (2) forms an oblique angle in relation to an optical axis (7) of the light emitted from the light source (1).
Spectrophotometric equipment according to claim 1 c h a r a c t e r i s e d i n, that the front wall (5) of the cuvette (2) forms an oblique angle in relation to said optical axis (7) and that the rear wall (6) forms a substantially right angle in relation to said optical axis (7) .
3. Spectrophotometric equipment according to claim 1 or 2 c h a r a c t e r i s e d i n, that the cuvette is wedge-shaped having its largest distance between the front wall (5) and the rear wall (7) upwards
4. Spectrophotometric equipment according to any of the claims 1 - 3 c h a r a c t e r i s e d i n, that the light source (1) works within a wavelength area of 800 to 1100 nm.
5. Spectrophotometric equipment according to any of the claims 1 - 4 c h a r a c t e r i s e d i n, that the sample material (4) is present in form of granules, a powder, a slurry, a paste or a liquid.
6. Spectrophotometric equipment according to claim 5 c h a r a c t e r i s e d i n, that the sample material (4) is present in form of a finely grin¬ ded powder.
7. Spectrophotometric equipment according to any of the claims 1 - 6 c h a r a c t e r i s e d i n, that at least the front wall (5) and the rear wall (6) is made of a material being transparent to used wavelengths.
8. Spectrophotometric equipment according to claim 7 c h a r a c t e r i s e d i n, that at least the front wall (5) and the rear wall (6) is made of glass or quartz.
9. Use of a spectrophotometric equipment according to any of the claims 1 - 8 for analyses of a material being available in form of granules, a powder, a slurry, a paste or a liquid or for analyses of a material being transformed into granu¬ les, a powder, a slurry, a paste or a liquid prior to said analyses.
10. Use according to claim 9 c h a r a c t e r i s e d i n, that the material is a fodder or a foodstuff.
11. Use according to claim 9 or 10 c h a r a c t e r i s e d i n, that the material contains fat and/or protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU29413/95A AU2941395A (en) | 1994-07-04 | 1995-06-30 | Spectrophotometric equipment and use thereof |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9402354A SE502995C2 (en) | 1994-07-04 | 1994-07-04 | Spectrophotometer equipment and its use |
| SE9402354-6 | 1994-07-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1996001417A1 true WO1996001417A1 (en) | 1996-01-18 |
Family
ID=20394618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/SE1995/000808 WO1996001417A1 (en) | 1994-07-04 | 1995-06-30 | Spectrophotometric equipment and use thereof |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU2941395A (en) |
| SE (1) | SE502995C2 (en) |
| WO (1) | WO1996001417A1 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5893288A (en) * | 1997-04-25 | 1999-04-13 | Morgan Construction Company | Multiple outlet finishing mill |
| WO2000012997A1 (en) * | 1998-08-26 | 2000-03-09 | Alfa Laval Agri Ab | Fluid sensing device and method for use in particular in milking machines |
| US6573988B1 (en) | 1997-10-31 | 2003-06-03 | Foss Electric A/S | Cuvette and spacer therefor as well as a method of producing the spacer |
| CN110044829A (en) * | 2019-05-08 | 2019-07-23 | 上海仪电物理光学仪器有限公司 | Food quality analyzer and its application method |
| EP4049756A1 (en) * | 2021-02-24 | 2022-08-31 | Paul Marienfeld GmbH & Co. KG | Container for a sample and method for analyzing a sample with the container |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4056324A (en) * | 1975-05-10 | 1977-11-01 | Hildegard Gohde | Apparatus for counting and/or measuring particles suspended in a fluid medium |
| WO1982000358A1 (en) * | 1980-07-24 | 1982-02-04 | Oy Labsystems | Method of measurement and a cuvette |
| EP0479231A1 (en) * | 1990-10-01 | 1992-04-08 | Canon Kabushiki Kaisha | Apparatus and method for measuring specimen |
| WO1993015390A1 (en) * | 1992-01-22 | 1993-08-05 | Matthew Jonathan Rooney | Assessment of characteristics of fluids |
-
1994
- 1994-07-04 SE SE9402354A patent/SE502995C2/en not_active IP Right Cessation
-
1995
- 1995-06-30 WO PCT/SE1995/000808 patent/WO1996001417A1/en active Application Filing
- 1995-06-30 AU AU29413/95A patent/AU2941395A/en not_active Abandoned
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4056324A (en) * | 1975-05-10 | 1977-11-01 | Hildegard Gohde | Apparatus for counting and/or measuring particles suspended in a fluid medium |
| WO1982000358A1 (en) * | 1980-07-24 | 1982-02-04 | Oy Labsystems | Method of measurement and a cuvette |
| EP0479231A1 (en) * | 1990-10-01 | 1992-04-08 | Canon Kabushiki Kaisha | Apparatus and method for measuring specimen |
| WO1993015390A1 (en) * | 1992-01-22 | 1993-08-05 | Matthew Jonathan Rooney | Assessment of characteristics of fluids |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5893288A (en) * | 1997-04-25 | 1999-04-13 | Morgan Construction Company | Multiple outlet finishing mill |
| US6573988B1 (en) | 1997-10-31 | 2003-06-03 | Foss Electric A/S | Cuvette and spacer therefor as well as a method of producing the spacer |
| WO2000012997A1 (en) * | 1998-08-26 | 2000-03-09 | Alfa Laval Agri Ab | Fluid sensing device and method for use in particular in milking machines |
| CN110044829A (en) * | 2019-05-08 | 2019-07-23 | 上海仪电物理光学仪器有限公司 | Food quality analyzer and its application method |
| EP4049756A1 (en) * | 2021-02-24 | 2022-08-31 | Paul Marienfeld GmbH & Co. KG | Container for a sample and method for analyzing a sample with the container |
| WO2022180089A3 (en) * | 2021-02-24 | 2022-10-20 | Paul Marienfeld GmbH & Co. KG | Container for a sample and method for examining a sample with the container |
Also Published As
| Publication number | Publication date |
|---|---|
| SE9402354L (en) | 1996-01-05 |
| SE502995C2 (en) | 1996-03-04 |
| SE9402354D0 (en) | 1994-07-04 |
| AU2941395A (en) | 1996-01-25 |
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