WO1996041176A1 - Dosage immunologique pour determiner l'activation des cellules du sang - Google Patents
Dosage immunologique pour determiner l'activation des cellules du sang Download PDFInfo
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- WO1996041176A1 WO1996041176A1 PCT/US1996/008776 US9608776W WO9641176A1 WO 1996041176 A1 WO1996041176 A1 WO 1996041176A1 US 9608776 W US9608776 W US 9608776W WO 9641176 A1 WO9641176 A1 WO 9641176A1
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- Prior art keywords
- platelets
- activation
- platelet
- enzyme
- antibody
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- 239000002245 particle Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- QGVLYPPODPLXMB-QXYKVGAMSA-N phorbol Natural products C[C@@H]1[C@@H](O)[C@]2(O)[C@H]([C@H]3C=C(CO)C[C@@]4(O)[C@H](C=C(C)C4=O)[C@@]13O)C2(C)C QGVLYPPODPLXMB-QXYKVGAMSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000036470 plasma concentration Effects 0.000 description 1
- 238000003615 platelet activation assay Methods 0.000 description 1
- 239000000106 platelet aggregation inhibitor Substances 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- GMVPRGQOIOIIMI-DWKJAMRDSA-N prostaglandin E1 Chemical compound CCCCC[C@H](O)\C=C\[C@H]1[C@H](O)CC(=O)[C@@H]1CCCCCCC(O)=O GMVPRGQOIOIIMI-DWKJAMRDSA-N 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000006973 regulation of leukocyte migration Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000246 remedial effect Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 239000003868 thrombin inhibitor Substances 0.000 description 1
- 239000003634 thrombocyte concentrate Substances 0.000 description 1
- DSNBHJFQCNUKMA-SCKDECHMSA-N thromboxane A2 Chemical compound OC(=O)CCC\C=C/C[C@@H]1[C@@H](/C=C/[C@@H](O)CCCCC)O[C@@H]2O[C@H]1C2 DSNBHJFQCNUKMA-SCKDECHMSA-N 0.000 description 1
- XNRNNGPBEPRNAR-JQBLCGNGSA-N thromboxane B2 Chemical compound CCCCC[C@H](O)\C=C\[C@H]1OC(O)C[C@H](O)[C@@H]1C\C=C/CCCC(O)=O XNRNNGPBEPRNAR-JQBLCGNGSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
Definitions
- the assay of the present invention can eliminate the need for any equipment other than that commonly found in an ordinary clinical/or research laboratory.
- the following description deals with a platelet assay, but the procedure is also applicable to samples of other types of cells which undergo activation, for example, blood cells other than red blood cells, such as monocytes, lymphocytes, and granulocytes.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La présente invention décrit un procédé de détermination du niveau d'activation des plaquettes et autres types de cellules du sang qui sont soumises à une activation par la technique ELISA ou par d'autres techniques de dosage immunologique. Ce procédé consiste à faire réagir un échantillon contenant les cellules du sang dans une phase liquide avec une quantité excessive d'anticorps primaires spécifiques à l'activation avant de permettre aux cellules dans l'échantillon de se lier à une surface solide. Cette procédure diffère des procédures traditionnelles d'ELISA, dans lesquelles les cellules sont fixées à la surface d'un tube à essai en plastique ou à une plaque de microtitrage avant d'ajouter l'anticorps primaire. Selon l'invention, il s'est révélé que le fait de lier les cellules à une surface avant la réaction avec l'anticorps primaire modifie le niveau d'activation des cellules rendant ainsi le dosage moins précis. En outre, la technique ELISA sur les plaquettes entières peut, tout comme les analyses cytométriques de flux, détecter par sensibilité les plaquettes activées.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96918058A EP0838034A4 (fr) | 1995-06-07 | 1996-06-05 | Dosage immunologique pour determiner l'activation des cellules du sang |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US47352095A | 1995-06-07 | 1995-06-07 | |
| US08/473,520 | 1995-06-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1996041176A1 true WO1996041176A1 (fr) | 1996-12-19 |
Family
ID=23879872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/008776 WO1996041176A1 (fr) | 1995-06-07 | 1996-06-05 | Dosage immunologique pour determiner l'activation des cellules du sang |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP0838034A4 (fr) |
| WO (1) | WO1996041176A1 (fr) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993021527A1 (fr) * | 1992-04-22 | 1993-10-28 | Milwaukee Heart Research Foundation | Dosage immunologique pour determiner l'activation de cellules sanguines |
-
1996
- 1996-06-05 WO PCT/US1996/008776 patent/WO1996041176A1/fr not_active Application Discontinuation
- 1996-06-05 EP EP96918058A patent/EP0838034A4/fr not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993021527A1 (fr) * | 1992-04-22 | 1993-10-28 | Milwaukee Heart Research Foundation | Dosage immunologique pour determiner l'activation de cellules sanguines |
Non-Patent Citations (1)
| Title |
|---|
| See also references of EP0838034A4 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0838034A1 (fr) | 1998-04-29 |
| EP0838034A4 (fr) | 2000-05-24 |
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