WO1996041007B1 - Detection de deletion de l'y de l'infertilite chez l'homme a l'aide d'amorces multiplex combinees - Google Patents
Detection de deletion de l'y de l'infertilite chez l'homme a l'aide d'amorces multiplex combineesInfo
- Publication number
- WO1996041007B1 WO1996041007B1 PCT/US1996/009421 US9609421W WO9641007B1 WO 1996041007 B1 WO1996041007 B1 WO 1996041007B1 US 9609421 W US9609421 W US 9609421W WO 9641007 B1 WO9641007 B1 WO 9641007B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- daz
- seq
- nos
- mic2
- primer pairs
- Prior art date
Links
- 238000012217 deletion Methods 0.000 title claims abstract 7
- 208000007466 Male Infertility Diseases 0.000 title claims abstract 5
- 238000001514 detection method Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 20
- 210000002593 Y chromosome Anatomy 0.000 claims abstract 7
- 238000007403 mPCR Methods 0.000 claims abstract 7
- 230000037430 deletion Effects 0.000 claims abstract 6
- 210000001182 human Y chromosome Anatomy 0.000 claims abstract 3
- 230000035772 mutation Effects 0.000 claims abstract 2
- 102000008482 12E7 Antigen Human genes 0.000 claims 32
- 108010020567 12E7 Antigen Proteins 0.000 claims 32
- 108091034117 Oligonucleotide Proteins 0.000 claims 24
- 239000008280 blood Substances 0.000 claims 14
- 210000004369 blood Anatomy 0.000 claims 14
- 101001088879 Homo sapiens Lysine-specific demethylase 5D Proteins 0.000 claims 12
- 102100033143 Lysine-specific demethylase 5D Human genes 0.000 claims 12
- 239000013611 chromosomal DNA Substances 0.000 claims 10
- 239000012634 fragment Substances 0.000 claims 10
- 101001088887 Homo sapiens Lysine-specific demethylase 5C Proteins 0.000 claims 8
- 102100033249 Lysine-specific demethylase 5C Human genes 0.000 claims 8
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 6
- 239000011777 magnesium Substances 0.000 claims 6
- 229910052749 magnesium Inorganic materials 0.000 claims 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 6
- -1 BKM Proteins 0.000 claims 4
- 101000915402 Homo sapiens Deleted in azoospermia protein 1 Proteins 0.000 claims 4
- 101000742844 Homo sapiens RNA-binding motif protein, Y chromosome, family 1 member A1 Proteins 0.000 claims 4
- 101000964566 Homo sapiens Zinc finger Y-chromosomal protein Proteins 0.000 claims 4
- 102100038040 RNA-binding motif protein, Y chromosome, family 1 member A1 Human genes 0.000 claims 4
- 102100040802 Zinc finger Y-chromosomal protein Human genes 0.000 claims 4
- 101150116409 dys-1 gene Proteins 0.000 claims 4
- 210000003917 human chromosome Anatomy 0.000 claims 4
- 230000037452 priming Effects 0.000 claims 4
- 238000004925 denaturation Methods 0.000 claims 3
- 230000036425 denaturation Effects 0.000 claims 3
- 238000001502 gel electrophoresis Methods 0.000 claims 3
- 108010006785 Taq Polymerase Proteins 0.000 claims 2
- 238000000137 annealing Methods 0.000 claims 2
- 206010003883 azoospermia Diseases 0.000 claims 2
- 230000001351 cycling effect Effects 0.000 claims 2
- 239000002480 mineral oil Substances 0.000 claims 2
- 235000010446 mineral oil Nutrition 0.000 claims 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims 1
- 229920000936 Agarose Polymers 0.000 claims 1
- 238000000246 agarose gel electrophoresis Methods 0.000 claims 1
- 208000000509 infertility Diseases 0.000 claims 1
- 230000036512 infertility Effects 0.000 claims 1
- 231100000535 infertility Toxicity 0.000 claims 1
- 208000008634 oligospermia Diseases 0.000 claims 1
- 230000036616 oligospermia Effects 0.000 claims 1
- 231100000528 oligospermia Toxicity 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000035558 fertility Effects 0.000 abstract 1
Abstract
La présente invention concerne un procédé permettant de tester l'intégrité d'un chromosome Y par des réactions PCR multiplex amplifiant des régions spécifiques du chromosome Y humain pour lesquelles on a établi une relation avec la fertilité normale chez le sujet masculin. Ce procédé permet de détecter des mutations par délétion à l'intérieur du chromosome Y, mutations qui permettent de prédire une infertilité chez le sujet masculin. L'invention concerne également une trousse contenant les réactifs nécessaires à la mise en ÷uvre dudit procédé.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96919190A EP0832288A1 (fr) | 1995-06-07 | 1996-06-06 | Detection de deletion de l'y de l'infertilite chez l'homme a l'aide d'amorces multiplex combinees |
| AU61592/96A AU6159296A (en) | 1995-06-07 | 1996-06-06 | Male infertility y-deletion detection with multiplex primer combinations |
| JP9501742A JPH11507222A (ja) | 1995-06-07 | 1996-06-06 | マルチプレックスプライマー結合による男性不妊ｙ染色体欠失検出 |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/472,416 US5783390A (en) | 1995-06-07 | 1995-06-07 | Male infertility Y-deletion detection battery |
| US08/472,416 | 1995-06-07 | ||
| US08/531,556 US5776682A (en) | 1995-06-07 | 1995-09-18 | Male infertility y-deletion detection battery |
| US08/531,556 | 1995-09-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1996041007A1 WO1996041007A1 (fr) | 1996-12-19 |
| WO1996041007B1 true WO1996041007B1 (fr) | 1997-02-13 |
Family
ID=23875426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/009421 WO1996041007A1 (fr) | 1995-06-07 | 1996-06-06 | Detection de deletion de l'y de l'infertilite chez l'homme a l'aide d'amorces multiplex combinees |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US5783390A (fr) |
| EP (1) | EP0832288A1 (fr) |
| JP (1) | JPH11507222A (fr) |
| AU (1) | AU6159296A (fr) |
| CA (1) | CA2221521A1 (fr) |
| WO (1) | WO1996041007A1 (fr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5840549A (en) * | 1995-06-07 | 1998-11-24 | Promega Corporation | Male infertility Y-deletion detection battery |
| US6103886A (en) * | 1997-04-11 | 2000-08-15 | Whitehead Institute For Biomedical Research | Genes in the non-recombining region of the Y chromosome |
| US20020058265A1 (en) * | 2000-09-15 | 2002-05-16 | Promega Corporation | Detection of microsatellite instability and its use in diagnosis of tumors |
| US6844152B1 (en) * | 2000-09-15 | 2005-01-18 | Promega Corporation | Detection of microsatellite instability and its use in diagnosis of tumors |
| DE10160563B4 (de) * | 2001-12-10 | 2005-12-22 | Ogham Gmbh | Verfahren zur Untersuchung der erblichen Infertilität des Mannes |
| KR100464084B1 (ko) * | 2002-01-17 | 2005-01-03 | (주)지노첵 | 다기능성 프라이머를 이용하여 중합효소연쇄반응의 산물을확인하는 방법 |
| JP2008517601A (ja) * | 2004-10-22 | 2008-05-29 | プロメガ コーポレイション | 生殖細胞のゲノム不安定性を検出するための方法及びキット |
| CN113684268B (zh) * | 2021-08-17 | 2023-08-29 | 北京嘉宝仁和医疗科技股份有限公司 | Y染色体azf区域微缺失检测的方法、引物组合物及试剂盒 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0261172A1 (fr) * | 1986-02-21 | 1988-03-30 | Whitehead Institute For Biomedical Research | Sondes d'hybridation de l'adn specifique du chromosome y et procedes d'utilisation desdites sondes |
| WO1989002440A2 (fr) * | 1987-09-21 | 1989-03-23 | Whitehead Institute For Biomedical Research | Sondes d'hybridation a adn specifiques a l'y et utilisations prevues a cet effet |
| AU7947794A (en) * | 1993-10-22 | 1995-05-08 | Medical Research Council | Azoospermia identification and treatment |
-
1995
- 1995-06-07 US US08/472,416 patent/US5783390A/en not_active Expired - Fee Related
-
1996
- 1996-06-06 EP EP96919190A patent/EP0832288A1/fr not_active Withdrawn
- 1996-06-06 WO PCT/US1996/009421 patent/WO1996041007A1/fr not_active Application Discontinuation
- 1996-06-06 AU AU61592/96A patent/AU6159296A/en not_active Abandoned
- 1996-06-06 CA CA002221521A patent/CA2221521A1/fr not_active Abandoned
- 1996-06-06 JP JP9501742A patent/JPH11507222A/ja active Pending
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