WO1996040949B1 - Induction of male sterility in plants by expression of high levels of avidin - Google Patents
Induction of male sterility in plants by expression of high levels of avidinInfo
- Publication number
- WO1996040949B1 WO1996040949B1 PCT/US1996/008583 US9608583W WO9640949B1 WO 1996040949 B1 WO1996040949 B1 WO 1996040949B1 US 9608583 W US9608583 W US 9608583W WO 9640949 B1 WO9640949 B1 WO 9640949B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- plant
- promoter
- nucleotide sequence
- dna molecule
- avidin
- Prior art date
Links
- 108090001008 Avidin Proteins 0.000 title claims abstract 17
- 206010021929 Infertility male Diseases 0.000 title claims 3
- 208000007466 Male Infertility Diseases 0.000 title claims 3
- 230000006698 induction Effects 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 12
- 230000009261 transgenic effect Effects 0.000 claims abstract 10
- 239000013604 expression vector Substances 0.000 claims abstract 9
- 230000035558 fertility Effects 0.000 claims abstract 2
- 241000196324 Embryophyta Species 0.000 claims 29
- 239000002773 nucleotide Substances 0.000 claims 19
- 125000003729 nucleotide group Chemical group 0.000 claims 19
- 108020004414 DNA Proteins 0.000 claims 17
- 102000053602 DNA Human genes 0.000 claims 17
- 108090000623 proteins and genes Proteins 0.000 claims 9
- 108091026890 Coding region Proteins 0.000 claims 4
- 239000012634 fragment Substances 0.000 claims 4
- 108090000848 Ubiquitin Proteins 0.000 claims 3
- 102000044159 Ubiquitin Human genes 0.000 claims 3
- 230000000692 anti-sense effect Effects 0.000 claims 3
- 108020004999 messenger RNA Proteins 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 108090000994 Catalytic RNA Proteins 0.000 claims 2
- 230000001939 inductive effect Effects 0.000 claims 2
- 108020005544 Antisense RNA Proteins 0.000 claims 1
- 102000053642 Catalytic RNA Human genes 0.000 claims 1
- 241001057636 Dracaena deremensis Species 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 244000020551 Helianthus annuus Species 0.000 claims 1
- 101710196632 LexA repressor Proteins 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 239000003184 complementary RNA Substances 0.000 claims 1
- 230000001172 regenerating effect Effects 0.000 claims 1
- 108091092562 ribozyme Proteins 0.000 claims 1
- 238000005507 spraying Methods 0.000 claims 1
- 108700026220 vif Genes Proteins 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
Abstract
Male-sterile plants can be produced by increasing the endogenous concentration of avidin in the plant tissues. This effect can be achieved by producing transgenic plants containing an expression vector in which a promoter is operably linked to a DNA sequence encoding avidin. Methods for restoring male fertility are also disclosed.
Claims
1. An isolated DNA molecule comprising a nucleotide sequence encoding avidin operably linked to a plant promoter sequence.
2. An expression vector comprising the isolated DNA molecule of claim 1.
3. An expression vector according to claim 2, wherein said plant promoter sequence is a constitutive promoter.
4. An expression vector according to claim 3, wherein said constitutive promoter sequence is an ubiquitin promoter sequence.
5. A transgenic plant, wherein said plant comprises a heterologous nucleotide sequence comprising an avidin coding sequence, wherein said heterologous nucleotide sequence increases the concentration of avidin in the tissues of the plant, and whereby said increased concentration of avidin causes said plant to be male sterile.
6. A transgenic plant according to claim 5, wherein said plant is a corn plant.
7. A transgenic plant according to claim 5, wherein said plant is a soybean plant.
8. A transgenic plant according to claim 5, wherein said plant is a sunflower plant.
9. A transgenic plant according to claim 5, wherein said heterologous nucleotide sequence further comprises a constitutive promoter sequence.
10. A transgenic plant according to claim 9, wherein said constitutive promoter sequence is a ubiquitin promoter sequence.
11. A method of producing a transgenic male-sterile plant, comprising (1) introducing into plant cells an expression vector comprising a promoter and an avidin coding sequence, wherein said promoter controls the expression of said avidin coding sequence; and (2) regenerating a transgenic plant from said plant cells wherein expression of said avidin coding sequence in the plant causes male sterility. -41-
12. The method of claim 11, wherein said promoter comprises a ubiquitin promoter.
13. A method of using an avidin gene to produce a male- fertile hybrid plant, comprising the steps of:
(a) producing a first parent male-sterile plant comprising a DNA molecule according to claim 1, wherein the expression of avidin causes male sterility;
(b) producing a second transgenic parent plant expressing a second foreign gene; and
(c) cross-fertilizing said first parent with said second parent to produce a hybrid plant, wherein said hybrid plant expresses said second foreign gene, and wherein the product of said second foreign gene reduces expression of avidin in said hybrid plant, thereby producing a male-fertile hybrid plant.
14. The method of claim 13, wherein said second foreign gene is selected from the group consisting of an antisense gene, a ribozyme gene and an external guide sequence gene.
15. The method of claim 14, wherein said antisense gene encodes an antisense RNA that complements avidin mRNA sequences.
16. The method of claim 14, wherein said ribozyme gene encodes a ribozyme which is designed to target avidin mRNA molecules.
17. The method of claim 14, wherein said external guide sequence gene comprises sequences which complement avidin mRNA sequences.
18. The method of claim 14, wherein said DNA molecule of said first parent plant further comprises a LexA operator which is operatively linked to the promoter of the DNA molecule, and wherein said second foreign gene encodes the LexA repressor.
19. The expression vector of claim 2, wherein said promoter sequence is an anther-specific promoter sequence comprising an anther box selected from the group consisting of: -42-
(a) a DNA molecule having the nucleotide sequence of SEQ ID NO:1;
(b) a DNA molecule having the nucleotide sequence of SEQ ID NO:2;
(c) a DNA molecule having the nucleotide sequence of SEQ ID NO:3; and
(d) a functional fragment of (a) , (b) or (c) .
20. The expression vector of claim 19, wherein said anther box has the nucleotide sequence of SEQ ID NO:l, and wherein said anther-specific promoter further comprises a core promoter selected from the group consisting of:
(a) CaMV 35S core promoter;
(b) a DNA molecule having the nucleotide sequence of SEQ ID NO:4;
(c) a DNA molecule having the nucleotide sequence of SEQ ID NO:5;
(d) a DNA molecule having the nucleotide sequence of SEQ ID NO:6; and
(e) a functional fragment of (b) , (c) or (d) .
21. The expression vector of claim 19, wherein said anther box has the nucleotide sequence of SEQ ID NO:2, and wherein said anther-specific promoter further comprises a core promoter selected from the group consisting of:
(a) CaMV 35S core promoter;
(b) a DNA molecule having the nucleotide sequence of SEQ ID NO:4;
(c) a DNA molecule having the nucleotide sequence of SEQ ID NO:5;
(d) a DNA molecule having the nucleotide sequence of SEQ ID NO:6; and
(e) a functional fragment of (b) , (c) or (d) .
22. The expression vector of claim 19, wherein said anther box has the nucleotide sequence of SEQ ID NO:6, and wherein said anther-specific promoter further comprises a core promoter selected from the group consisting of: (a) CaMV 35S core promoter;
(b) a DNA molecule having the nucleotide sequence of SEQ ID NO:4;
(c) a DNA molecule having the nucleotide sequence of SEQ ID NO:5;
(d) a DNA molecule having the nucleotide sequence of SEQ ID NO:6; and
(e) a functional fragment of (b) , (c) or (d) .
23. A method for restoring fertility in a plant rendered male sterile by expression of avidin, comprising spraying said plant with a solution of biotin.
24. The method of claim 11, wherein said promoter comprises an inducible promoter.
25. The method of claim 14, wherein said antisense gene comprises an inducible promoter.
-44-
STATEMENT UNDER ARΗCLE 19
Pursuant to Article 19(1) and Rule 46.1, applicant hereby amends the claims of the above-captioned application and files the following statement with the amendment.
Attached hereto are replacement pages 36 through 39 for the application. The claims presented herein closely conform to the claims in the allowed U.S. priority application, which will issue shortly.
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RO97-02278A RO120270B1 (en) | 1995-06-07 | 1996-06-06 | Inducing male sterility at plants, by avidin high level expression |
| CA002223460A CA2223460C (en) | 1995-06-07 | 1996-06-06 | Induction of male sterility in plants by expression of high levels of avidin |
| AU61493/96A AU708618B2 (en) | 1995-06-07 | 1996-06-06 | Induction of male sterility in plants by expression of high levels of avidin |
| BR9609069A BR9609069A (en) | 1995-06-07 | 1996-06-06 | Induction of male sterility in plants by expression of high levels of avidin |
| EP96919051A EP0832261A2 (en) | 1995-06-07 | 1996-06-06 | Induction of male sterility in plants by expression of high levels of avidin |
| JP9501127A JPH11512922A (en) | 1995-06-07 | 1996-06-06 | Induction of plant male sterility by high levels of avidin expression |
| PL96323745A PL323745A1 (en) | 1995-06-07 | 1996-06-06 | Method of causing male sterility with plants by high avidin expression |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US47558295A | 1995-06-07 | 1995-06-07 | |
| US08/475,582 | 1995-06-07 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1996040949A1 WO1996040949A1 (en) | 1996-12-19 |
| WO1996040949B1 true WO1996040949B1 (en) | 1997-02-20 |
Family
ID=23888227
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/008583 WO1996040949A1 (en) | 1995-06-07 | 1996-06-06 | Induction of male sterility in plants by expression of high levels of avidin |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP0832261A2 (en) |
| JP (1) | JPH11512922A (en) |
| KR (1) | KR19990022728A (en) |
| CN (1) | CN1192784A (en) |
| AR (1) | AR002352A1 (en) |
| AU (1) | AU708618B2 (en) |
| BR (1) | BR9609069A (en) |
| CA (1) | CA2223460C (en) |
| HU (1) | HUP9900885A3 (en) |
| PL (1) | PL323745A1 (en) |
| RO (1) | RO120270B1 (en) |
| WO (1) | WO1996040949A1 (en) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5837850A (en) * | 1994-04-21 | 1998-11-17 | Pioneer Hi-Bred International, Inc. | Regulatory element conferring tapetum specificity |
| US5962769A (en) * | 1995-06-07 | 1999-10-05 | Pioneer Hi-Bred International, Inc. | Induction of male sterility in plants by expression of high levels of avidin |
| US5767379A (en) * | 1995-11-06 | 1998-06-16 | John Howard | Commercial production of avidin in plants |
| US7071384B2 (en) | 1997-03-07 | 2006-07-04 | Genencor International, Inc. | Methods for commercial production of heterologous laccase in plant tissue and extraction of the laccase from plant seed |
| CA2281830C (en) | 1997-03-07 | 2007-06-19 | Prodigene, Inc. | Methods of commercial production and extraction of protein from seed |
| AU2585297A (en) * | 1997-03-20 | 1998-09-22 | Prodigene, Inc. | Methods of commercial production and extraction of protein from seed |
| US6046385A (en) * | 1997-10-09 | 2000-04-04 | Midwest Oilseeds, Inc. | Mutant male sterile gene of soybean |
| BR9912814A (en) | 1998-07-15 | 2001-05-02 | Horticulture & Food Res Inst | Chimeric polypeptides allowing the expression of harmful plant proteins |
| IL125632A0 (en) * | 1998-08-03 | 1999-04-11 | Israel State | Degeneration and restoration of plant tissue |
| CN1329009A (en) * | 2000-06-19 | 2002-01-02 | 上海博德基因开发有限公司 | A novel polypeptide-avidin 9 and polynucleotide for coding this polypeptide |
| AU2002248325A1 (en) * | 2001-01-04 | 2002-07-16 | Fleit, Lois | Method to maintain a genic male-sterile female parental lines for the production of hybrid wheat |
| US20020129399A1 (en) * | 2001-03-09 | 2002-09-12 | Marc Albertsen | Biotin-binding compounds for induction of sterility in plants |
| CN101851281B (en) | 2001-03-12 | 2012-07-04 | 日本烟草产业株式会社 | A novel protein, a gene encoding therefor and a method of using the same |
| CA2646399A1 (en) * | 2006-04-11 | 2007-10-18 | The State Of Israel, Ministry Of Agriculture & Rural Development, Agricu Ltural Research Organization, (A.R.O.), Volcani Center | Plant gene promoter and its use |
| WO2010046422A2 (en) | 2008-10-22 | 2010-04-29 | Basf Se | Use of auxin type herbicides on cultivated plants |
| AR075465A1 (en) | 2008-10-22 | 2011-04-06 | Basf Se | USE OF SULFONILUREA HERBICIDES IN CULTIVATED PLANTS |
| EP2903437A1 (en) | 2012-10-01 | 2015-08-12 | Basf Se | Use of n-thio-anthranilamide compounds on cultivated plants |
| WO2014079820A1 (en) | 2012-11-22 | 2014-05-30 | Basf Se | Use of anthranilamide compounds for reducing insect-vectored viral infections |
| WO2015161744A1 (en) * | 2014-04-22 | 2015-10-29 | 未名兴旺系统作物设计前沿实验室（北京）有限公司 | Identification and use of promoter ptaasg048 specifically expressed by plant anther |
| EP3028573A1 (en) | 2014-12-05 | 2016-06-08 | Basf Se | Use of a triazole fungicide on transgenic plants |
| WO2016091674A1 (en) | 2014-12-12 | 2016-06-16 | Basf Se | Use of cyclaniliprole on cultivated plants |
| BR112017021450B1 (en) | 2015-04-07 | 2021-12-28 | Basf Agrochemical Products B.V. | PEST CONTROL METHODS, PLANT HEALTH IMPROVEMENT METHOD AND COATED SEED |
| EP3338552A1 (en) | 2016-12-21 | 2018-06-27 | Basf Se | Use of a tetrazolinone fungicide on transgenic plants |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1986002077A1 (en) * | 1984-10-02 | 1986-04-10 | Meade Harry M | Production of streptavidin-like polypeptides |
| WO1994000992A1 (en) * | 1992-07-10 | 1994-01-20 | Pioneer Hi-Bred International, Inc. | Avidin and homologues as larvicides against insect pests |
| US5583210A (en) * | 1993-03-18 | 1996-12-10 | Pioneer Hi-Bred International, Inc. | Methods and compositions for controlling plant development |
| US5633438A (en) * | 1994-11-22 | 1997-05-27 | Pioneer Hi-Bred International | Microspore-specific regulatory element |
-
1996
- 1996-06-06 KR KR1019970709170A patent/KR19990022728A/en not_active Abandoned
- 1996-06-06 JP JP9501127A patent/JPH11512922A/en not_active Ceased
- 1996-06-06 CA CA002223460A patent/CA2223460C/en not_active Expired - Fee Related
- 1996-06-06 PL PL96323745A patent/PL323745A1/en unknown
- 1996-06-06 EP EP96919051A patent/EP0832261A2/en not_active Withdrawn
- 1996-06-06 HU HU9900885A patent/HUP9900885A3/en unknown
- 1996-06-06 BR BR9609069A patent/BR9609069A/en not_active Application Discontinuation
- 1996-06-06 RO RO97-02278A patent/RO120270B1/en unknown
- 1996-06-06 WO PCT/US1996/008583 patent/WO1996040949A1/en not_active Application Discontinuation
- 1996-06-06 AR ARP960102983A patent/AR002352A1/en unknown
- 1996-06-06 AU AU61493/96A patent/AU708618B2/en not_active Ceased
- 1996-06-06 CN CN96196046A patent/CN1192784A/en active Pending
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