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WO1996040414A1 - Systeme d'enzymes pour le traitement de gaz - Google Patents

Systeme d'enzymes pour le traitement de gaz Download PDF

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Publication number
WO1996040414A1
WO1996040414A1 PCT/US1996/009610 US9609610W WO9640414A1 WO 1996040414 A1 WO1996040414 A1 WO 1996040414A1 US 9609610 W US9609610 W US 9609610W WO 9640414 A1 WO9640414 A1 WO 9640414A1
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WIPO (PCT)
Prior art keywords
phase
enzyme
gas
selected gas
zone
Prior art date
Application number
PCT/US1996/009610
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English (en)
Inventor
Michael C. Trachtenberg
Original Assignee
Trachtenberg Michael C
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Publication date
Application filed by Trachtenberg Michael C filed Critical Trachtenberg Michael C
Priority to EP96918364A priority Critical patent/EP0830196A4/fr
Priority to AU61045/96A priority patent/AU6104596A/en
Priority to CA002222030A priority patent/CA2222030C/fr
Publication of WO1996040414A1 publication Critical patent/WO1996040414A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/46—Removing components of defined structure
    • B01D53/62—Carbon oxides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/10—Single element gases other than halogens
    • B01D2257/102—Nitrogen
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/10—Single element gases other than halogens
    • B01D2257/104—Oxygen
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/20—Halogens or halogen compounds
    • B01D2257/206—Organic halogen compounds
    • B01D2257/2064—Chlorine
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/20—Halogens or halogen compounds
    • B01D2257/206—Organic halogen compounds
    • B01D2257/2066—Fluorine
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/30—Sulfur compounds
    • B01D2257/302—Sulfur oxides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/30—Sulfur compounds
    • B01D2257/304—Hydrogen sulfide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/40—Nitrogen compounds
    • B01D2257/404—Nitrogen oxides other than dinitrogen oxide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/50—Carbon oxides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/70—Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
    • B01D2257/702—Hydrocarbons
    • B01D2257/7022—Aliphatic hydrocarbons
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Definitions

  • This invention relates to a process utilizing natural, modified or engineered enzymes as agents, alone or immobilized in conjunction with membranes or other techniques or cells or cell fragments for the extraction of one or more specific molecules from a mixture of molecules in a first gaseous phase and moving at least one specific molecule to a second phase.
  • the mixture of molecules is in a first gas phase and a specific molecule type is transported to a second mobile fluid phase.
  • the specific gases to be separated may be present in a gas mixture, or in solution of mixed gases.
  • the specific gases can be treated either in their dissolved or solvated molecular state or after they have been converted to an ionic or ionized state either by reaction with water, reaction with another solvent, or by other prior chemical reaction.
  • the invention process may also be used to regenerate a specific gas from the second phase.
  • the invention further relates to the extraction of gases, molecules or ions from a liquid solution, aqueous or non-aqueous, to concentrate or isolate specific materials alone or in chemical combination and in ionic or non-ionic form.
  • Traditional gas separation means commonly exhibit one or more of the following problems: they are energy inefficient, commonly nonspecific, quite slow, require a relatively pure feedstock, depend on a significant pressure head, or use ecologically questionable or toxic compounds.
  • the relatively pure feed stock requirement may result in a geographical restriction of available feed materials. The geographic availability may require shipment from distant locations such that transportation costs may be high, and even prohibitive for some uses.
  • the preceding limitations present restrictions on the growth and application of gas extraction/purification systems. A gas separation or enrichment process that did not require highly concentrated feed-stocks thus eliminating or reducing transportation requirements would be beneficial.
  • enzymes present several advantages including enhanced efficiency, speed, and increased specificity. Enzymes also commonly distinguish optical isomers. Further, they can be used at moderate temperatures and pressures, enhancing safety.
  • the invention provides a process for gas separation wherein a selected gas in a mixed gas stream is contacted by an enzyme, and the selected gas is removed from the gas stream.
  • the selected gas may also be recovered as a separated gas.
  • the invention provides a process for gas treatment wherein a selected gas in a gas phase is contacted by a solvated enzyme wherein the enzyme active site is in direct contact with the gas phase, the selected gas is converted to a first product in a condensed phase by contact with the solvated enzyme.
  • the hydrated enzyme is partially covered with a hydrocarbon film.
  • the hydrocarbon film is a lipid layer, more preferably the lipid layer is a phospholipid and most preferably the phospholipid is a bilayer.
  • the first product is further contacted with a second enzyme in the condensed phase and converted to a second product, which maybe the originally selected gas or a different material.
  • the invention provides a process for gas treatment wherein a selected gas in a gas phase s contacted by a solvated enzyme wherein the enzyme active site is in direct contact with the gas phase, the selected gas is converted to a first product in a condensed phase by contact with the solvated enzyme, the first product is further contacted with a second enzyme in the condensed phase and converted to a second product.
  • a simplified bioreactor which comprises buoyant beads coated with enzyme, floating on the surface of a condensed fluid phase, in contact with a gas phase such that the active site of a portion of the enzyme coating is in direct contact with the gas phase, and the beads are free to rotate in response to motion in either phase, and means for producing motion in at least one phase such that portions of the beads alternately contact the gas phase and the fluid phase, bringing a selected gas into the condensed fluid phase.
  • the enzyme is immobilized on the bead surface.
  • the enzyme is carbonic anhydrase, and the condensed fluid phase is water.
  • the invention also provides novel apparatus for practicing the process for gas separations.
  • the method couples use of selective enzymes and immobilization by several distinct means in conjunction with a variety of support structures or membranes to achieve gas enrichment, purification, separation and processing of gas mixtures.
  • the process of the invention can be used in a selective or subtractive manner, and provides on location separation and purification, thereby avoiding transportation problems.
  • the invention also encompasses advanced enzyme fixation methods resulting in higher enzyme activity than the crude cross linking techniques fixation of Bonaventura et al.
  • the present invention is the first to provide enzyme immobilization with active site exposure directly to gas phase substrates.
  • the invention is a process to achieve vectorial movement and separation of one or more gas substrates admixed with several other gases, thus isolating a selected gas from a mixture.
  • the invention may be used to detect and measure the gas, to subsequently purifying the selected gas, or to convert selected gases to new chemical entities.
  • an appropriate enzyme or enzymes are present at the supply side to effect a change in state or chemistry of the selected gas and thereby generate a reaction product and reduce or prevent exit of the enzyme reaction product back to the gaseous supply side.
  • Solubility and concentration factors favor movement of the enzyme reaction product into a second phase which also contacts the enzyme.
  • the second phase may be a second gas stream, or a solvent or a solvent doped with ions or other chemical materials to facilitate retention of the enzyme reaction product. Further treatment of the second phase may recover the selected gas as a purified or concentrated stream, or further react the selected gas or gases to produce a desired chemical entity.
  • the several objects the invention are to provide a process wherein gas mixtures are effectively separated so that at least one species may be isolated and/or enriched. Another object is to extract a selected gas for independent use or to serve as a start material or substrate for another physical, chemical or biological operation. Another object is to process ionic equivalents of a gas, present in a solution, to extract the ionic gas equivalents in the form of a gas or to use the material as a substrate for another physical, chemical or biological operation. Another object of the present invention is to allow serial, parallel or sequential processing of gas mixtures for sequential isolation or enrichment of gases. Another object is to allow very large volumes of gas to be produced, not heretofore possible.
  • Further objects of the present invention are to utilize a low purity gas source. Yet another object is to treat the effluent from any kind of smoke stack, stationary or mobile, to process gases including carbon dioxide, oxides of nitrogen, oxides of sulfur, hydrogen sulfide, methane, ozone, or chlorofluorocarbons. Carbon dioxide removal from gas streams is a particularly preferred objective for gas processing.
  • Fig. 1 illustrates a simplified bioreactor for separation of a selected gas.
  • Fig. 2 shows a reactor set up for recovery of carbon dioxide from a flue gas stream.
  • Fig. 3 shows a multienzyme reactor for production of urea.
  • Fig.4 shows a reactor for conversion of oxygen and water to hydrogen peroxide.
  • Fig. 5 illustrates buoyant beads for positioning enzyme at a liquid surface to facilitate transfer of a selected gas to a liquid phase.
  • Fig. 6 Phase rotating immobilized enzyme carrier, plan view.
  • Fig. 7 Phase rotating immobilized enzyme carrier, side view. n .TAi .Fn nFSCRIPTION OF THE INVENTION
  • Figure 1 illustrates an apparatus for practicing the invention as a process for gas separation wherein a selected gas in a mixed gas stream is contacted by an enzyme, and the selected gas is removed from the gas stream.
  • the apparatus allows the selected gas to be recovered as a separated gas.
  • the bioreactor comprises a vessel having at least one first wall 2 and at least one second wall 4, and an optional end wall 6 which closes a portion of the volume enclosed by first wall 2 and second wall 4.
  • a portion 9 of second wall 4 is permeable to at least one selected gas and also comprises a support surface 8 for enzyme immobilization; a mixed gas stream 10, from a gas source not shown, enters a space between first wall 2 and second wall 4 wherein the mixed gas stream 10 contacts immobilized enzyme 12 fixed to support surface 8 which removes selected gas 14 from mixed gas stream 10; selected gas 14 interacts with immobilized enzyme 12 and is caused to enter second phase 16 which is in fluid contact with immobilization support 8 and immobilized enzyme 12 and confined with the volume enclosed by second wall 4 and optionally end wall 6; second phase 16 may be a low pressure carrier gas stream or vacuum line, or a liquid or gas permeable gel, alternatively second phase 16 may be stirred by optional stirrer 18 or circulated by an optional pump, not illustrated.
  • An appropriate enzyme or enzymes are present at permeable portion 9 to effect a change of state or chemistry of selected gas 14 and thereby reduce of prevent exit of the selected gas 14 back to the supply side.
  • the desired vectorial movement of selected gas 14 into phase 16 may be promoted by concentration effects, solvation, chemical conversion, ionization, or other means to favor movement of selected gas 14 or its enzyme reaction product 15 into second phase 16.
  • second phase 16 may flow through die area enclosed by second walls 4 by use of a suitable port, not shown, and second phase 16 may also be recirculated through a recycling loop not shown.
  • the selected gas 14 or its enzyme reaction product 15 may be used in second phase or may optionally be further processed.
  • Additional support surface 8, permeable area 9 and immobilized enzyme may be provided in second wall 4 to connect second phase 16 to a recovery zone 22 enclosed by first wall 2, or second wall 4, or a combination thereof, and optionally end wall 6 and recovered as a further reacted product or as a concentrated gas by, for example, reverse reaction with the same immobilized enzyme to generate a recovered purified selected gas 14.
  • a concentration gradient or other means will be used to favor vectorial movement of selected gas 14 or enzyme reaction product 15 from second phase 16 to recovery zone 22. Any of the means previously listed can be also be applied at this stage to provide movement into recovery zone 22.
  • one or more different enzymes or other catalysts may be placed between second phase 16 and recovery zone 22 to further convert selected gas 14 to a new product.
  • Figure 2 illustrates a reactor removing carbon dioxide from a mixed gas stream such as animal respiratory gases by contact with carbonic anhydrase, conversion of carbon dioxide to bicarbonate, and subsequently reconverting bicarbonate to carbon dioxide as a concentrated gas stream.
  • Figure 3 illustrates a multienzyme reactor system which converts carbon dioxide and ammonia to urea.
  • the mixed gas stream 10 may be pretreated to provide an optimal temperature or pressure, or to remove components that would lower the reactor's efficiency, or contribute components that may pass through the selection media to contaminate purified selected gas stream 24, or recovered product 25.
  • pretreatment include mechanical screening by filters, chemical screening by adsorbents or absorbents, or scrubbing, and use of heat exchangers, waste heat recovery processing, compression, expansion and other gas processing steps known in die art.
  • the immobilization support 8 maybe of any conventional material such as polysaccharide surfaces or gels, ion exchange resin, treated silicon oxides, poms metal structures, carbon rods or tubes, graphite fibers, silica beads, cellulose membranes, gel matrices such as polyacrylamide gels, poly(acryloyI morpholine) gels, nylon or other polymer meshes, or other suitable binding surface.
  • the enzyme may be wholly or partially encapsulated in a suitable material such as cellulose nitrate capsules, polyvinyl alcohol capsules, starch capsules or liposome preparations.
  • the enzyme may be fixed at a phase boundary, as illustrated in Figures 5, 6 and 7 or by use of nonionic surfactants as described, for example in Li et al., U.S. Patent 3,897,308.
  • the enzyme may be fixed to the surface by binding, covalent bonding, physical attraction, coordination bonds, chelation, or other binding means or mechanical trapping or o er means known to d ose skilled in the art.
  • the support may also be a membrane of selective permeability. The selectivity may be by size, or other characteristic.
  • the membrane may be a lipid bilayer doped with passive porins, channels or ionophores of the co-porter or antiporter type which commonly rely on properties such as charge an ⁇ Vor hydrated radius for
  • permeable portion 9 and support surface 8 include permeable membranes, natural and artificial, including semipermeable plastic membranes, black lipid membranes, alternatively doped with ionophores to provide ion conducting channels.
  • the porins may be active, i.e. , dependent on an energy flux.
  • the energy flux may be tied to an endogenous high energy compound such as a labile triphosphate bond or to an exogenous supply of energy via photons, electrons or protons.
  • the immobilization support works as membrane with selective permeability to maintain separation of the mixed gas stream 10 from the second phase 16 while passing selected gas 5 14 into contact with second phase 16.
  • the enzymes immobilized on support surface 8 are of two types, simple enzymes and those requiring cofactors for activity.
  • Simple enzymes may be fixed to the support surface by any of the means known to the art.
  • the enzymes are modified by adding an amino acid sequence that binds to the 0 support without substantially reducing enzyme activity.
  • the enzyme is modified by altering the DNA segment coding for the enzyme to add a sequence coding for an amino acid sequence that yields a binding moiety to the enzyme in a manner that enhances enzyme binding.
  • the modification may provide a sequence that binds to a metal such as a poly-
  • histidine sequence or may code for an epitope or antigen moiety that binds to an antibody or may be a portion of an antibody that binds a known antigen.
  • a poly histidine sequence can be added to an enzyme such as carbonic anhydrase by splicing a DNA fragment coding for the desired polyhistidine sequence into the DNA coding for the enzyme at either terminus of the protein sequence, and expressing the DNA in a suitable organism and recovering the new enzyme.
  • the cofactor in the case of enzymes requiring cofactors the cofactor maybe supplied in second phase 16 or also fixed to the support surface 8 or supplied by pretreatment of surface 8 with the cofactor to activate bound enzyme. If multiple materials are to be bound to the support surface 8 they may be exposed to a prepared surface sequentially or as a mixture.
  • the second phase 16 may be selected from the group consisting of gases, aqueous solvents, protic solvents, aprotic solvents, hydrocarbon solvents, aromatic hydrocarbon solvents and supercritical fluids.
  • the second phase may be stirred or a flow introduced by a pump to move fresh material into contact with the immobilized enzyme at the support surface.
  • a flow may be introduced into second phase 16 by a stirrer or pump or other conventional means. Flow or other means is applied to maintain a concentration or other gradient to produce vectorial movement of the selected gas 14 into condensed phase 16.
  • immobilization support 8 and film 26 maybe combined as, for example, in a lipid bilayer wherein a portion of the enzyme extends into the lipid bilayer to provide both support and an evaporation barrier.
  • a film 26 is provided in which the selected gas is soluble, and which decreases the escape of second phase 16 into mixed gas stream 10.
  • the film maybe a gel, hydrocarbon layer, or preferably a lipid or phospholipid layer or bilayer.
  • the condensed phase 16 is contacted by a second surface which may carry a catalyst, enzyme or simply be selectively permeable such that selected gas 14 or its equivalent or derivative is transported to recovery zone 22. Again a concentration or other gradient may be used to enhance vectorial movement from second phase 16 into recovery zone 22.
  • Gases are defined as materials which are in the gas phase at ambient temperature and pressure (taken to be 20 degrees C. and one atmosphere). Suitable gases include nitrogen, oxygen, oxides of carbon, nitrogen, sulfur, methane, ammonia, hydrogen sulfide and the like. Any gas that interacts with an enzyme directly from the gas phase may be a selected gas 14 in combination with a suitable enzyme 12 which interacts with the selected gas 14.
  • An enzyme is a protein or peptide that selectively binds or reacts with a gas molecule.
  • An 5_ enzyme is composed of amino acids with an active site which binds a specific molecule and facilities a change in the bound molecule.
  • An enzyme may include nonnaturally occurring amino acids, and maybe natural or artificial.
  • Suitable enzymes include: ENZYME EC NUMBER 0 glucose oxidase 1.1.3.4 aldehyde oxidase 1.2.3.1 hydroxylamine oxidase 1.7.3.4 sulfite oxidase 1.8.3.1 sulfur-ferric ion 1.8.99.- 5 oxidoreductase catechol oxidase 1.1.3.14
  • cytochrome carbon monoxide- 1.2.3.- me ⁇ iylene blue methylene blue oxidoreductase carbon monoxide 1.2.99.2 methyl viologen dehydrogenase nitrate reductase (NADH) 1.6.6.1 NADH nitrate reductase 1.6.6.2 NAD(P)H
  • NADP(P)H sur ⁇ roxide-forming 1.6.99.- NADPH enzyme
  • a membrane or mesh surface such as, for example, nylon
  • the material is activated by exposure to a 0.2 M nitric acid wash.
  • the activated surface is exposed to 2 M sodium carbonate solution containing 20 g of disodium irninodiacetic acid (IDA) for 24 0 h at 60-65 C.
  • IDA disodium irninodiacetic acid
  • the material is then rinsed with 0.1 M sodium carbonate, 0.01 M sodium acetate and water. It is then charged using zinc chloride at 1 mg/ml.
  • the basis of protein retention is binding of amino acids on the protein surface (notably histidine) to the vacant coordination sites 0 of metals (e.g., copper, zinc) complexed to chelating groups such as N-(carboxymethyl) glycine.
  • the IDA ligand is favored because it is resistant to Ph between 2 and 13, and temperatures to at least 120 C.
  • a carbonic anhydrase (CA) (E 5 4.2.1.1) is immobilized on both sides of a reservoir of aqueous buffer.
  • CA is the one of the fastest enzymes known with a turnover number of 600,000 katals. Thus it has the ability to catalyze the hydration of 600,000 molecules of carbon dioxide per second per molecule of CA.
  • Carbonic anhydrase is a diffusion limited enzyme. Thus, any CO 2 molecule which comes
  • the mixed gas supply is taken from the exhaust stack of a gas fired electric power plant following appropriate heat exchangers and after passage of the exhaust through an air house.
  • the make up of a typical gas is N 2 - 64.92%, O 2 - 2.04%, CO 2 - 7.06%, and H 2 O - 25.98%.
  • CA contains a zinc atom and other divalent cations can, under correct circumstances, displace the zinc and alter the reactivity of the enzyme.
  • Cobalt is a particularly potent divalent cation of this class.
  • the enzyme is covered with a thin oil film 26 to prevent water loss from the second phase 16.
  • the second phase is an aqueous 20 mM sodium phosphate buffer.
  • CA catalyzes the hydration of CO 2 to HCO 3 -.
  • the bicarbonate is delivered to the flowing buffer stream of the second phase 16 and carried to contact the permeable wall area adjacent recovery one 22 where it contacts a second layer of CA fixed to support surface 8 at permeable wall area 9.
  • the CA layer reverses the reaction and converts HCO 3 - to CO 2 .
  • the second phase 16 buffer stream is constantly flowing to deliver lean buffered medium to the bicarbonate rich zone adjacent to the mixed gas supply 10 and then carry the bicarbonate rich fluid to the surface adjacent the recovery zone 22.
  • the flow removes bicarbonate from the first surface where it can act as a competitive inhibitor of CA and slow the reaction.
  • the overall effect is to create a continuous removal of product adjacent the enzyme surfaces and maintain a gradient that favors vectorial movement of CO 2 into solution as bicarbonate adjacent mixed gas stream 10 and from bicarbonate to CO 2 adjacent zone 22.
  • Carbonic anhydrase has a dehydrating rate constant which is slightly slower than the hydrating rate constant. For equal molar volumes of carbon dioxide and bicarbonate this favors the hydration of carbon dioxide. However, adjusting the relative surface area of the two enzyme surfaces will compensate for this difference. The newly generated CO 2 is drawn into recovery zone 22 at low pressure for extraction. This guarantees a vectorial flow for the entire system. The kinetics and the specificity of the enzyme greatly favor the removal 5 of CO 2 from the mixed gas supply above all other gases. Carbonic anhydrase does not recognize or interact with any of the other gases in the flue gas mixture. However, under steady state conditions the concentration and solubility of the several gases in the mixed gas stream will determine the degree to which they may pollute the purified gas stream.
  • the solubility of these gases in oil and in water at 37UQs given in Table I The permeability of CO 2 in water is 210 * 10 '9 * cm 3 (STP) * cm/sec * cm 2 * cm-Hg.
  • the diffusivity of CO 2 in water is 1.96 * 10 "5 * cm 2 /sec.
  • the solubility of CO 2 in water is 3.39 * 10 "5 gmoles * cm * 10 "3 /atm.
  • FIG 3 a two stage reactor of the same general design and operation as that illustrated in Example 2 is shown.
  • the inlet mixed gas 10 is preferably a carbon dioxide rich stream.
  • carbonic anhydrase is the immobilized enzyme 12 which is covered by a lipid film 26 as in Example 0 2.
  • Carbon dioxide is converted to bicarbonate adjacent the mixed gas side also as described in Example 2.
  • a separate supply line 28 injects ammonia into the aqueous phase 16, which is converted to ammonium carbonate by conventional chemical reaction with the bicarbonate.
  • the ammonium carbonate rich aqueous phase is circulated to contact urease 12 immobilized on a semipermeable 5 membrane 9 adjacent urea recovery zone 22.
  • ammonium carbonate from the aqueous buffer is converted to urea by urease and transported across the membrane to the recovery zone via absorption.
  • An organic solvent in the recovery zone dissolves the urea which is conveyed away from permeable zone 9, for recovery.
  • the urea stream is flash evaporated to concentrate the urea
  • the operation of a gas phase to gas phase bioreactor is illustrated in Fig 4.
  • the immobilized enzyme 12 is catalase and the selected gas from mixed gas stream 10 is oxygen.
  • Catalase is a diffusion limited or "perfect enzyme". Its reaction rate is about 5.6*10 6 mol/mol/min. ⁇ G for the reaction of hydrogen peroxide to produce oxygen and water in the uncatalyzed state is 18 kcal mol. When catalyzed by a typical physical catalyst, platinum, this value drops to 13 kcal/mol, an improvement of only 38%. However, when catalyzed by catalase it declines dramatically to 7 kcal/mol, an improvement over platinum of 86% and a total improvement of 257%. This reaction is exothalmic in me amount of 45.68 kcal/gmol in aqueous phase and 25.97 kcal/gmol in gas phase.
  • the reaction can be forced to proceed in the opposite direction, using O 2
  • H 2 O 2 H 2 O 2
  • the raw materials O 2 and H 2 O vapor are supplied at elevated temperature, or other means, sufficient to overcome the energy barrier, then H 2 O 2 can be generated. It is important to maintain this reaction in the gas phase first to minimize the energy differences and second to prevent the deleterious effect of even moderate concentrations of peroxide on 0 the integrity of the enzyme. It is also important that the second side is at lower pressure than the first side to provide vectorial flow to the system and remove the peroxide from the enzyme before an appreciable concentration accumulates. Due to its specificity catalase is unreactive to other gases commonly found in air, for example. Water vapor is supplied to the enzyme by increasing the humidity 5 of the mixed gas stream 10, preferably to near 100%. Water vapor at near 100% humidity provides sufficient water to hydrate the active site of the enzyme. The consequence of the exemplified operation is the production of enriched peroxide in the second phase 16.
  • FIG. 5 A simplified bioreactor is illustrated in Fig. 5 wherein an enzyme 12 such as carbonic anhydrase is coated on the surface of a buoyant bead 30 which floats at the surface of a fluid.
  • an enzyme 12 such as carbonic anhydrase is coated on the surface of a buoyant bead 30 which floats at the surface of a fluid.
  • a gas stream contacts the exposed enzyme on the exposed surface of the floating bead, and the enzyme binds its gas substrate. Turbulence of the fluid at the surface rotates the bead to immerse enzyme with
  • the net effect is to facilitate the conversion of carbon dioxide in the mixed gas to bicarbonate in aqueous solution.
  • a bead reactor may be incorporated for example into a conventional scrubber to reduce the amount of carbon dioxide released into the atmosphere by a fossil fuel fired power generation plant. Beads floating on the surface of a pond may be useful to
  • oblong beads 32 which may be partially coated with enzyme and weighted to maintain the enzyme exposed to the gas phase.
  • An alternate embodiment uses fully coated beads 30 of a selected density to float at the fluid surface with a portion of the 0 enzyme directly exposed to the gas phase. While any fluid may be employed provided that the enzyme remains active and product is washed off into the fluid phase, water or aqueous buffers are the preferred fluids.
  • An alternative embodiment uses a structure similar to that shown in Fig. 6.
  • Beads having enzyme immobilized on the surface are fixed to a lattice 34 in 5 such a manner that the beads can rotate around a lattice element.
  • the lattice 34 is positioned such that the beads are fixed at the surface of a fluid phase in contact with a gas stream.
  • the gas stream impinges on the beads such that the force of the gas stream 10 is sufficient to rotate the beads exposing fresh enzyme surface to the gas and carrying bound gas into contact with the fluid phase 16.
  • the turbulence is such that the bead 30 carrying the immobilized enzyme rotates to be successively exposed to the gas phase and the fluid phase 16.
  • the bioreactor illustrated in Figs. 6 and 7 utilizes countercurrent flow of the two phases on opposite sides of the immobilized enzyme affixed to axially 5 tethered bead-like immobilization surfaces. Under these circumstances the beads will rotate moving the surface from contacting the first fluid to contacting the second fluid and vice versa. Gases or products will be delivered from d e first fluid to the second fluid where they will be deposited.
  • the effect of the immobilized enzyme at the bead surface is to facilitate transfer of a gas or
  • the countercurrent fluids are immiscible or are separated by a semipermeable layer which may partially overlay the bead support lattice 34 to separate the countercurrent fluids.
  • the beads are sized to extend beyond the separating layer and contact each fluid.
  • the beads of Figures 6 or 7 may include vanes to facilitate rotation by the fluid flow. Disks or other shaped supports may be substituted for spherical beads in this embodiment.
  • the choice of fluid, buffer and salt content is determined by both the solubility and ionization potential of ionic equivalents of the gas of interest in the fluid, on both an absolute scale and in relation to the solubility and formation of ionic equivalents of other gases in the mixed gas stream.
  • the following factors guide the choice.
  • One is to increase the fractional concentration of the gas of interest.
  • Another is to decrease the fractional concentration of contaminants.
  • Yet another is to increase the ability of chemical reactions to occur in the medium and to increase the ability of the enzyme to generate ionic equivalents.
  • Another is to optimize the miscibility of the gas with the phase surrounding the enzyme.

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Abstract

L'invention porte sur un procédé de séparation de gaz consistant à mettre un gaz sélectionné présent dans un courant d'un mélange de gaz en contact avec une enzyme présentant un site actif directement en contact avec le courant gazeux ce qui permet d'en extraire au moins une partie du gaz sélectionné. Dans un mode de réalisation, le gaz sélectionné est mis en contact avec une enzyme solvatée dont le site actif est directement en contact avec la phase gazeuse, le gaz sélectionné se trouvant converti en un premier produit en phase condensée par contact avec l'enzyme solvatée. L'invention porte également sur un bioréacteur comportant une cuve présentant au moins une première paroi délimitant une zone d'admission et une deuxième paroi délimitant une deuxième zone de phase. Une partie de la deuxième cloison est perméable à au moins l'un des gaz sélectionnés de la zone d'admission, mais retient la deuxième phase dans la zone de deuxième phase. Une partie de la deuxième cloison comporte également une surface support à laquelle est fixée au moins une enzyme si bien que le mélange de gaz pénétrant dans la zone d'admission entre en contact avec l'enzyme qui extrait un gaz sélectionné du mélange de gaz et transfert le gaz sélectionné dans la zone de seconde phase. L'invention porte en outre sur un autre bioréacteur comportant: des perles flottantes revêtues d'un enzyme flottant à la surface d'une phase fluide condensée et en contact avec une phase gazeuse de manière à ce que le site actif de l'enzyme soit en contact direct avec la phase gazeuse, les perles pouvant tourner librement en réponse aux mouvements de l'une ou l'autre phase. L'invention porte enfin sur un moyen mettant en mouvement au moins l'une des phases de manière à ce que des parties des perles entrent alternativement en contact avec la phase gazeuse et avec la phase fluide, ce qui amène le gaz sélectionné en phase fluide condensée.
PCT/US1996/009610 1995-06-07 1996-06-06 Systeme d'enzymes pour le traitement de gaz WO1996040414A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP96918364A EP0830196A4 (fr) 1995-06-07 1996-06-06 Systeme d'enzymes pour le traitement de gaz
AU61045/96A AU6104596A (en) 1995-06-07 1996-06-06 Enzyme systems for gas processing
CA002222030A CA2222030C (fr) 1995-06-07 1996-06-06 Systemes d'enzymes pour le traitement de gaz

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US48668995A 1995-06-07 1995-06-07
US08/486,689 1995-06-07

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EP1166846A1 (fr) * 2000-06-22 2002-01-02 Jose Martinez Martinez Procédè de traitment de gaz et résidus provenant d'installations de compostage et d'épuration d'eaux usées
WO2004028667A1 (fr) * 2002-09-27 2004-04-08 Co2 Solution Inc. Procede et installation pour le recyclage d'emissions de gaz carbonique a partir de centrales electriques en especes carbonees utiles
US7998714B2 (en) 2008-09-29 2011-08-16 Akermin, Inc. Process for accelerated capture of carbon dioxide
US8354261B2 (en) 2010-06-30 2013-01-15 Codexis, Inc. Highly stable β-class carbonic anhydrases useful in carbon capture systems
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US8871485B2 (en) 2010-06-23 2014-10-28 University Of Florida Research Foundation, Inc. Modified carbonic anhydrase enzymes and their use in carbon dioxide sequestration and elimination
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Cited By (18)

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Publication number Priority date Publication date Assignee Title
US6524843B1 (en) * 1997-06-04 2003-02-25 Co2 Solution Inc. Process and apparatus for the treatment of carbon dioxide with carbonic anhydrase
WO1998055210A1 (fr) * 1997-06-04 1998-12-10 Systemes Envirobio Inc. Procede et dispositif permettant de traiter le dioxyde de carbone par l'anhydrase carbonique
WO2001042259A1 (fr) * 1999-12-11 2001-06-14 British Nuclear Fuels Plc Composes de coordination 1,3,6-triazacyclohexane servant a la preparation de polymeres a base de metal
EP1166846A1 (fr) * 2000-06-22 2002-01-02 Jose Martinez Martinez Procédè de traitment de gaz et résidus provenant d'installations de compostage et d'épuration d'eaux usées
WO2004028667A1 (fr) * 2002-09-27 2004-04-08 Co2 Solution Inc. Procede et installation pour le recyclage d'emissions de gaz carbonique a partir de centrales electriques en especes carbonees utiles
US7596952B2 (en) 2002-09-27 2009-10-06 Co2 Solution Inc. Process and a plant for recycling carbon dioxide emissions from power plants into useful carbonated species
US7998714B2 (en) 2008-09-29 2011-08-16 Akermin, Inc. Process for accelerated capture of carbon dioxide
US8178332B2 (en) 2008-09-29 2012-05-15 Akermin, Inc. Process for accelerated capture of carbon dioxide
US8846377B2 (en) 2009-08-04 2014-09-30 Co2 Solutions Inc. Process for CO2 capture using micro-particles comprising biocatalysts
US10220348B2 (en) 2009-08-04 2019-03-05 Co2 Solutions Inc. Process for CO2 capture using micro-particles comprising biocatalysts
US9480949B2 (en) 2009-08-04 2016-11-01 Co2 Solutions Inc. Process for desorbing CO2 capture from ion-rich mixture with micro-particles comprising biocatalysts
US8871485B2 (en) 2010-06-23 2014-10-28 University Of Florida Research Foundation, Inc. Modified carbonic anhydrase enzymes and their use in carbon dioxide sequestration and elimination
US8354261B2 (en) 2010-06-30 2013-01-15 Codexis, Inc. Highly stable β-class carbonic anhydrases useful in carbon capture systems
US8569031B2 (en) 2010-06-30 2013-10-29 Codexis, Inc. Chemically modified carbonic anhydrases useful in carbon capture systems
US8512989B2 (en) 2010-06-30 2013-08-20 Codexis, Inc. Highly stable beta-class carbonic anhydrases useful in carbon capture systems
US8420364B2 (en) 2010-06-30 2013-04-16 Codexis, Inc. Highly stable beta-class carbonic anhydrases useful in carbon capture systems
US8354262B2 (en) 2010-06-30 2013-01-15 Codexis, Inc. Chemically modified carbonic anhydrases useful in carbon capture systems
WO2022198278A1 (fr) * 2021-03-24 2022-09-29 The University Of Melbourne Perles flottantes contenant de l'anhydrase carbonique pour production d'algues

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