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WO1995034668B1 - Inhibition cytoplasmique de l'expression genique - Google Patents

Inhibition cytoplasmique de l'expression genique

Info

Publication number
WO1995034668B1
WO1995034668B1 PCT/US1995/006741 US9506741W WO9534668B1 WO 1995034668 B1 WO1995034668 B1 WO 1995034668B1 US 9506741 W US9506741 W US 9506741W WO 9534668 B1 WO9534668 B1 WO 9534668B1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
eukaryotic cell
rna
vector
cells
Prior art date
Application number
PCT/US1995/006741
Other languages
English (en)
Other versions
WO1995034668A2 (fr
WO1995034668A3 (fr
Filing date
Publication date
Priority claimed from US08/260,546 external-priority patent/US5922602A/en
Application filed filed Critical
Priority to AT95921458T priority Critical patent/ATE221574T1/de
Priority to MX9606476A priority patent/MX9606476A/es
Priority to JP8502208A priority patent/JPH10501968A/ja
Priority to CA002193094A priority patent/CA2193094C/fr
Priority to DE69527654T priority patent/DE69527654T2/de
Priority to EP95921458A priority patent/EP0804600B1/fr
Priority to AU26534/95A priority patent/AU710588B2/en
Publication of WO1995034668A2 publication Critical patent/WO1995034668A2/fr
Publication of WO1995034668A3 publication Critical patent/WO1995034668A3/fr
Publication of WO1995034668B1 publication Critical patent/WO1995034668B1/fr

Links

Abstract

Un aspect de l'invention concerne de nouvelles structures génétiques s'appliquant à l'expression de l'ARN inhibiteur dans le cytoplasme des cellules eucaryotiques. L'ARN inhibiteur peut être un ARN antisens ou un ARN co-suppresseur. Les structures génétiques de l'invention peuvent effectuer une réplication dans le cytoplasme d'une cellule eucaryotique et comporter une région promotrice en combinaison fonctionnelle avec un polynucléotide codant. Les structures génétiques peuvent être conçues de façon à effectuer une réplication dans le cytoplasme des cellules végétales, des cellules de levure et des cellules mammaliennes. Lorsque la cellule eucaryotique est une cellule végétale, la structure génétique est de préférence dérivée d'un virus végétal d'ARN. Les structures génétiques dérivées du virus végétal d'ARN peuvent utiliser un promoteur sous-génomique de virus végétal, y compris des promoteurs sous-génomiques provenant des tobamovirus en combinaison fonctionnelle avec la région codante de l'ARN. Un autre aspect de l'invention concerne des cellules comprenant les structures génétiques de l'invention et un organisme comprenant une pluralité de ces cellules. Un autre aspect de l'invention concerne des procédés de réduction de l'expression d'un gène spécifique dans les cellules eucaryotiques, c.-à-d. des procédés de production de cellules eucryotiques présentant des niveaux réduits d'expression d'un gène spécifique. Les procédés de l'invention consistent à transfecter une cellule ayant une structure génétique de l'invention dans laquelle la région codant l'ARN est spécifique dudit gène. Enfin, un autre aspect de l'invention concerne des cellules végétales produisant des niveaux élevés de phytoène caroténoïde. Les niveaux élevés de phytoène sont obtenus par l'inhibition de l'expression au niveau de l'enzyme phytoène dénaturase à l'aide des vecteurs de l'invention.
PCT/US1995/006741 1994-06-16 1995-05-26 Inhibition cytoplasmique de l'expression genique WO1995034668A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AT95921458T ATE221574T1 (de) 1994-06-16 1995-05-26 Cytoplasmatische inhibition des genexpression
MX9606476A MX9606476A (es) 1994-06-16 1995-05-26 Inhibicion citoplasmica de expresion de genes.
JP8502208A JPH10501968A (ja) 1994-06-16 1995-05-26 遺伝子発現の細胞質阻害
CA002193094A CA2193094C (fr) 1994-06-16 1995-05-26 Inhibition cytoplasmique de l'expression genique
DE69527654T DE69527654T2 (de) 1994-06-16 1995-05-26 Cytoplasmatische inhibition des genexpression
EP95921458A EP0804600B1 (fr) 1994-06-16 1995-05-26 Inhibition cytoplasmique de l'expression genique
AU26534/95A AU710588B2 (en) 1994-06-16 1995-05-26 The cytoplasmic inhibition of gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US260,546 1994-06-16
US08/260,546 US5922602A (en) 1988-02-26 1994-06-16 Cytoplasmic inhibition of gene expression

Publications (3)

Publication Number Publication Date
WO1995034668A2 WO1995034668A2 (fr) 1995-12-21
WO1995034668A3 WO1995034668A3 (fr) 1996-02-01
WO1995034668B1 true WO1995034668B1 (fr) 1996-04-18

Family

ID=22989605

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/006741 WO1995034668A2 (fr) 1994-06-16 1995-05-26 Inhibition cytoplasmique de l'expression genique

Country Status (12)

Country Link
US (6) US5922602A (fr)
EP (2) EP1087017B1 (fr)
JP (1) JPH10501968A (fr)
AT (2) ATE221574T1 (fr)
AU (1) AU710588B2 (fr)
CA (1) CA2193094C (fr)
DE (2) DE69534421T2 (fr)
ES (2) ES2180641T3 (fr)
IL (1) IL113955A0 (fr)
MX (1) MX9606476A (fr)
WO (1) WO1995034668A2 (fr)
ZA (1) ZA954451B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7622633B2 (en) 1997-12-23 2009-11-24 Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA

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