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WO1995030749A1 - Dispositif de presentation - Google Patents

Dispositif de presentation Download PDF

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Publication number
WO1995030749A1
WO1995030749A1 PCT/US1995/006324 US9506324W WO9530749A1 WO 1995030749 A1 WO1995030749 A1 WO 1995030749A1 US 9506324 W US9506324 W US 9506324W WO 9530749 A1 WO9530749 A1 WO 9530749A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
dna
biological source
card
affixed
Prior art date
Application number
PCT/US1995/006324
Other languages
English (en)
Inventor
Kary B. Mullis
Ronald M. Cook
Francois Ferre
Original Assignee
Stargene, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stargene, Inc. filed Critical Stargene, Inc.
Priority to AU25965/95A priority Critical patent/AU2596595A/en
Publication of WO1995030749A1 publication Critical patent/WO1995030749A1/fr

Links

Classifications

    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09F—DISPLAYING; ADVERTISING; SIGNS; LABELS OR NAME-PLATES; SEALS
    • G09F3/00—Labels, tag tickets, or similar identification or indication means; Seals; Postage or like stamps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Definitions

  • This invention relates to a device for exhibiting the nucleic acid of a known biological source.
  • the invention concerns collectible articles carrying a sample of the genetic code of a precise biological source.
  • Collectible articles or other memorabilia of known individuals typically carry a likeness in the form of a photograph or illustration of the individual, or other identifying information.
  • the content of collectible cards, for example, is typically artistic and informational, and is reproduced or published by imprinting on cardboard.
  • Unique articles of known individuals, an original autograph, handwritten letter, or possessions formerly owned by the individual are limited in number, and highly collectable.
  • the present invention overcomes this obstacle and provides a device, which is available for collectors, that exhibits nucleic acid, either DNA or RNA, of a known biological source, that of a human individual or a non-human source, such as animals, plants, or microorganisms.
  • a device which comprises a member and nucleic acid derived from a known biological source. The nucleic acid is affixed to the member.
  • the device comprises a first member and a second member, in which the nucleic acid is affixed to the second member.
  • the device further comprises identification means for communicating the identity of the biological source.
  • a locating means such as a phosphorescent label mixed with or chemically attached to the nucleic acid, is also provided in the invention for locating or visualizing the position of the nucleic acid on the device.
  • the device comprises a sufficient number of amplified polymorphic loci to be uniquely specific for said biological source.
  • Figure 1 is front plan view of the device.
  • Figure 2 is a rear plan view of the device.
  • Figure 3 is sectional view of the device taken along line A-A of Figure 1.
  • Figure 4 is a sectional view of the device. DETAILED DESCRIPTION OF THE INVENTION
  • a device 10 is provided for exhibiting or containing the nucleic acid 12 of a known biological source.
  • the device 10 comprises a member 14 and nucleic acid 12 affixed to the member 14.
  • the nucleic acid 12 is derived from a known biological source.
  • the device 10 further comprises identification means 16 for communicating the identity of the biological source.
  • identification means 16 are typically photographs, illustrations, likenesses, facsimile or original autographs, text or other indicia identifying the known biological source or describing characteristics of the DNA, such as various geometric representations of sequence data of the DNA in the form of arrays of colored or geometric elements symbolizing specific nucleotides and their sequence.
  • the identification means 16 of the invention includes a magnetic coding strip containing nucleotide sequence information for the nucleic acid comprising the device. Methods for incorporating information, such as nucleotide sequence data, into a magnetic coding strip and for placing a magnetic coding strip on the device are well known in the art (Caulastics, Daly City, CA) .
  • Locating means 18 of the invention include visual labels mixed with the nucleic acid.
  • Visual labels for use in the invention include, but are not restricted to, color labels, phosphorescent labels, and fluorescent labels (Handbook of Fluorescent Probes and Research Chemicals. Richard Haugland, 1994, publisher Molecular Probes) .
  • a phosphorescent label such as Powder Glow Pigment Gl (The Coulter Counter Company) or Liquitex ® Fluorescent Red or Fluroescent Blue (Binney & Smith, Easton, PA) or Cres-Lite ® Pigments (Crescent Bronze Powder Co., Chicago, IL)
  • a phosphorescent label such as Powder Glow Pigment Gl (The Coulter Counter Company) or Liquitex ® Fluorescent Red or Fluroescent Blue (Binney & Smith, Easton, PA) or Cres-Lite ® Pigments (Crescent Bronze Powder Co., Chicago, IL)
  • Powder Glow Pigment Gl The Coulter Counter Company
  • Liquitex ® Fluorescent Red or Fluroescent Blue Boney & Smith, Easton, PA
  • Cres-Lite ® Pigments Crescent Bronze Powder Co., Chicago, IL
  • the invention contemplates the placing of a hologram 20 as locating means 18 on the face of the member, the location of the hologram 20 indicating the location of the nucleic acid 12 on the device.
  • An embodiment of the device includes a hologram 20 as a hologram laminate sheet 20 in combination with a phosphorescent or other color label labeling means for locating the nucleic acid 12 in the device.
  • Methods and manufacturing practices for placing holograms on paper or plastic cards or substrates are well known in the art (Light Impressions, Inc. Santa Cruz, CA. ; American Banknote Corporation, New Jersey; Transilwrap, Chicago, IL) .
  • the member 14 of the invention contemplates structures, which are lightweight, suitable for exhibiting, containing or encapsulating the nucleic acid of a biological source. Typical members include but are not restricted to cards and jewelry.
  • Another embodiment of the invention involves a second member 22 attached to a first member 24.
  • the second member 22, not the first member 24, would comprise the nucleic acid 12 derived from the known biological source.
  • a phosphorescent label or equivalent color label locating means 18 is associated with the nucleic acid of the second member.
  • a hologram locating means is laminated in juxtaposition with the second member to facilitate locating the nucleic acid exhibited, carried, enclosed, or accommodated by the device.
  • the nucleic acid 12, typically DNA, is affixed to the device 10.
  • An embodiment of the invention contemplates affixing the nucleic acid to the device in such a manner that the nucleic acid is non-extractable from the device.
  • the DNA non-extractably affixed to the device is denatured, rendered unavailable for further amplification or any further expression. That is to say that the DNA affixed to the device is not copiable nor capable of serving as a template for any biochemical reactions based upon its sequence of nucleotides.
  • the DNA of the device is in a form which cannot be further manipulated, nor extracted from the device, nor sized or sequence analyzed.
  • a typical embodiment of the present invention would be a member 14 in the form of a card, as shown in Figures 1-4.
  • One embodiment of the card of the present invention is a card about 3.5 x 3.5 inches.
  • the card is formed from cardboard or plastic, which may be further laminated to protect the card from deterioration.
  • Methods and materials for manufacturing cards from paper and/or plastic are well known in the art as are methods for imprinting or embossing photographs, likenesses, text and other indicia upon such cards (Caulastics, Daly City, CA; Outlook Graphics Corporation, Neenah, WI) .
  • Photographic prints can be prepared by any of the known photographic techniques (Design and Type, San Francisco, CA) .
  • the present invention embodies nucleic acid derived from a known biological source, which nucleic acid is exhibited, carried, enclosed, or accommodated by the device.
  • the biological sources of nucleic acid used in the present invention can be human or non-human.
  • Non- human sources include vertebrate or invertebrate animals, plants, microorganisms and viruses.
  • a sufficient amount of nucleic acid is obtained from the tissue of a known biological source.
  • the tissue is a minute amount of dead skin cells obtained from a non-invasive procedure, such as a light scraping of the skin. Typical scraping procedures include scraping beneath a finger nail or from the bottom of the foot.
  • Sufficient tissue is also available from hair follicles or scraping the inside of the cheek.
  • the nucleic acid 12 affixed to the device 10 is obtained directly from the biological source.
  • the nucleic acid obtained from the biological source is amplified, that is, copied with an amplification technique, and the copied nucleic acid is affixed to the device.
  • the preparation of the DNA from the tissue of a biological source is achieved by isolating the DNA from the tissue and subjecting the nucleic acid to amplification techniques well known in the art .
  • the Polvmerase Chain Reaction by K.B. Mullis, F. Ferre, and Richard Gibbs (1994), publ. Birkhauser; Molecular Cloning (1988), Sambroock, Fritsh and Maniatis, Cold Spring Harbor Laboratory Press) .
  • the present invention prefers the PCR amplification technique (Mullis and Faloona, 1987) for preparing DNA for the device of the present invention
  • other methods of amplification or cloning find utility for preparing the nucleic acid of the device (G. Walker, PCR Methods and Applications Vol. 3, pp.
  • PCR provides the advantages of allowing minute amounts of DNA to be amplified to whatever quantity might be desired, simplicity of use, and economy of practice both in materials and labor.
  • the present invention employs samples of nucleic acid of a known biological source affixed to the member.
  • the device of the invention uses nucleic acid which has a level of specificity ranging from being unique for an individual of a biological source to being merely from a known biological source.
  • an embodiment of the invention uses DNA obtained from a biological source which is not necessarily unique for that biological source, the DNA being a copy of a portion of that biological source's DNA which is common to other biological sources.
  • the device of the invention comprises DNA, the DNA including sequences which have been characterized as being specifically from a known biological source.
  • Methods are well known in the art for obtaining DNA sequences that are specific for a biological source, which methods and sequences are well known in the art for classifying and distinguish that biological source from all other biological sources (The Polvmerase Chain Reaction, by K.B. Mullis, F. Ferre, and Richard Gibbs (1994), publ. Birkhauser).
  • the biological source of the DNA is identified by amplification with one or more primers of known origin.
  • DNA obtained from a fossil specimen and suspected to be from a dinosaur is characterized and identified by amplification with degenerate avian and/or reptilian primers.
  • Protocols for classifying the biological source of DNA using various probe and/or primer protocols are well known in the art of molecular archaeology/anthropology and molecular forensics (The Polvmerase Chain Reaction, by K.B. Mullis, F. Ferre, and Richard Gibbs (1994), publ. Birkhauser).
  • the device of the present invention comprises a sample of DNA unique for the known individual of that biological source from whose tissue the DNA is derived directly or through amplification of the directly derived DNA.
  • the device comprises a sample of DNA having a sufficient number of amplified polymorphic loci to be uniquely specific for an individual, such an individual being a human or a tissue, cellular, or body fluid remnant from an individual should that individual be deceased.
  • the invention employs a strategy in which sufficient variability in the amplified DNA is obtained to produce a composite profile which is unique to an individual, except for that individual's onozygotic twin.
  • the strategy relies on the knowledge that a number of loci in complex genomes are polymorphic and that DNA polymorphisms can be detected by PCR (Jeffreys et al., 1988) .
  • PCR PCR-derived DNA sequence
  • By amplifying a number of polymorphic DNA loci a DNA fingerprint is obtained (Risch and Devlin, 1992) which, like an individual's actual fingerprint, is highly specific for an individual.
  • Geno ic Fingerprinting uses any of a number of techniques well known in the art for producing genomic fingerprints (Botstein et al.Am. J. Hum. Genet. 32:314- 331) .
  • AP-PCR arbitrarily primed PCR method
  • Sequence polymorphisms can be detected using AP-PCR and reproducible fingerprints of complex genomes can be generated. Divergence of even a fraction of a percent between two genomes often results in a different fingerprint pattern because a somewhat different set of sites in the genome have the best matches with the primers.
  • PCR products that are shared between only some individuals act as polymorphic markers. Each primer gives a different pattern of AP-PCR products, each with the potential of detecting polymorphisms between strains.
  • the present invention typically uses three primer sequences that are polymorphic in humans.
  • Three typical AP-PCR primers include:
  • primers typically give after amplification about forty amplicons of which seven on average will be polymorphic in humans. These three primers can be used alone or in pairwise combinations. In the pairwise reaction more than half the AP-PCR products are different from those produced by either oligo alone. Therefore, about 3 amplicons will represent new polymorphic sequences when the primers are used in pairwise combinations. Thus, with only three primers and their respective three pairwise combinations, an average of 30 sequence polymorphisms can be generated. These sequence polymorphisms which can be analyzed by sizing product using gel electrophoresis mostly correspond to deletion/insertion polymorphisms. Most of these markers are dimorphic (Jeffreys et al. 1988) . Assuming independence between these loci and a partition, on average, of 50:50 for the dimorphic markers in the human population, the chances that two individuals share the same DNA fingerprint using 30 sequence polymorphisms is about one in a billion.
  • nucleic acid affixed to the member can be made even more unique or specific by mixing the AP-PCR derived nucleic acid with nucleic acid amplified from another region which is highly polymorphic, namely, mitochondrial DNA, as described below.
  • the Mitochondrial DNA (mtDNA Control Region) control region The hypervariable segments of the human mitochondrial DNA (mtDNA) control region have been amplified and reveal an enormous amount of variability, with 274 mtDNA types observed among 525 individuals by using 23 sequence-specific ol. onucleotide (SSO) probes (Stoneking et al. Am. J. Hum. Genet. 48:370-382 (1991)).
  • SSO sequence-specific ol. onucleotide
  • the variability at the level of the nucleotide sequence is much higher, making it almost unique for a given individual.
  • the uniqueness of mtDNA at the nucleotide sequence level will allow the device of the invention to include highly specific nucleotide sequences.
  • the invention achieves this level of uniqueness by using asymmetric PCR in which unequal primer ratios are used to generate single-stranded templates suitable for direct sequencing. While not restricted to the following primer, the invention contemplates using these mtDNA primers (ibid) :
  • the device of the invention comprises a DNA fingerprint 12 of an individual affixed to the member 14 by preparing a mixture of DNAs derived from an individual.
  • the device of the invention typically comprises about a million DNA molecules with an average size of about 200 base pairs (BP) .
  • BP base pairs
  • Each DNA fingerprint contains about 200 different DNA fragments.
  • the mixture which includes AP-PCR loci and the mtDNA control region of an individual, is an absolutely unique DNA fingerprint for a given individual.
  • Skin or Integument Dried or dead skin cells, ready to be sluffed off or already sluffed off. This type of material is easily obtained from around the finger nail or from the sole of the foot.
  • Hair A shed hair provides up to 10 nanograms (ng) of genomic DNA. In a freshly-plucked hair, the root end may contain as much as 500 ng DNA. The hair shaft contains too little DNA to determine its quantity and condition. Recovery of purified DNA, however, usually yields no more than 200 ng DNA per freshly-plucked hair (Higuchi et al. 1988). There is less DNA in hair previously plucked because of DNA degradation by oxidation and other attacks of the environment. Only about 1 ng of DNA per hair would be available.
  • Tissue Paraffin-embedded tissues obtained at autopsy or during surgical interventions are cut to give 10 micron sections and several sections provide enough material for DNA extraction (Shibata, 1988) .
  • Bone/Teeth Teeth and bones, particularly those available from archaeological explorations, provide enough DNA for PCR. Such techniques are well known by those skilled in the art of molecular archaeology (The Polvmerase Chain Reaction, by K.B. Mullis, F. Ferre, and Richard Gibbs (1994) , publ. Birkhauser) . For non-human biological sources, in which the species is either extant or extinct, this small amount of DNA is readily obtainable either in tissues from various laboratories or commercial sources, or as isolated DNA, unamplified or amplified (George Poinar, Jr.. , Dept.
  • the device 10 of the invention comprises locating means 18 for locating the nucleic acid 12 which is exhibited, carried, enclosed, or accommodated by the device 10.
  • the locating means 18, typically a visual label, is mixed with the nucleic acid as described below under "Preparation of Fluorescent DNA Glass Beads.”
  • the device of the invention comprises nucleic acid
  • the fixation of the nucleic acid to the member or second member for exhibiting, carrying, enclosing or accommodating of the nucleic acid by the device can be achieved by methods well known in the art, including but not restricted to covalently attaching the nucleic acid to the member (E. Fahy, Nucleic Acids Research. Vol. 21: 1819 (1993)). Accordingly, the DNA, for example, affixed to the member cannot be chemically extracted from the member.
  • the device of the present invention includes an embodiment in which the nucleic acid is dissolved in an aqueous solution comprising a color label, such as ink or other pigment.
  • aqueous solution of nucleic acid is affixed to the device by printing on the member or second member of the device.
  • an embodiment of the invention comprises a second member 22 attached to a first member 24 such that the device exhibits, contains, encloses or accommodates the nucleic acid.
  • the nucleic acid 12 is affixed to the second member 22.
  • the first member 24 is a card and the second member 22 is a wafer formed from plastic-like material.
  • the second member 22 includes DNA 12 and locating means 18.
  • the second member 22 of the invention in the form of a plastic disk-shaped wafer comprises DNA 12 adsorbed onto a substrate which is mixed with a phosphorescent label or fluorescent label (R. Haugland, 1994) locating means 18 and embedded into a second substrate formed, from plastic, for example, as a disk-shaped wafer second member 22.
  • Methods for immobilizing DNA onto various substrates which are in turn incorporated onto other substrates are well known in the art, as follows. Preparation of Fluorescent DNA Glass Beads. DNA adsorbed glass beads are prepared by suspending 100 grams of glass beads (100 mesh) Alltech Associates, Deerfield, IL) in an aqueous solution of DNA.
  • the DNA may be directly bonded to a fluorescent species prior to its absorption onto the solid substrate.
  • a fluorescent species such as fluorescent substituted primers in the PCR amplification process (Mullis, 1994) .
  • the glass bead solid substrate may be substituted for by other materials such as plastic beads (Bangs Labs, Carmel, IND) or by fibers such as plastic or cotton thread (A. Lewis Thread Co., NY, NY).
  • the DNA solid substrate above may be conveniently immobilized in a clear plastic carrier such as a wafer disk as a second member 22.
  • a clear plastic carrier such as a wafer disk as a second member 22.
  • Such disk may be prepared in a variety of ways. DNA glass beads are suspended in a liquid polyester casting resin (Clear-Lite, Tap Plastics, Dublin, CA) followed by catalytic initiation to produce the solid wafer. Alternatively, pre-made wafer disks 22 may be drilled out to form a cavity in which the DNA - solid substrate is placed.
  • the second member 22 is attached to the first member 24 by various methods well known in the art for attaching plastic to cardboard or plastic to plastic, including, but not restricted to press fitting, lamination vis laminate sheets 26, ultrasonic welding, gluing, mechanically fastening, hot stamp foil, or friction fitting (Caulastics, Daly City, CA; Outlook Graphics, Corp., Neenah, WI; Transilwrap, Chicago, IL) .
  • the second member 22 disk is laminated to the first member card 24.
  • the member or first member of the device can be the surface or packaging surface of a compact disk, a video cassette, book or other gift, memorable or collectible article to which the second member comprising the DNA is attached with attaching means as described above.
  • Another embodiment of the invention involves the device of the invention having attachment means for attaching the device to a the surface or packaging surface of a compact disk, video cassette, book or other gift, memorable, or collectible article.
  • the attachment means are well known in the art, including, but not restricted to methods well known in the art for attaching plastic to cardboard or plastic to plastic, such as press fitting, lamination, ultrasonic welding, gluing, mechanically fastening, hot stamp foil, or friction fitting (Caulastics, Daly City, CA; Outlook Graphics, Corp., Neenah, WI; Transilwrap, Chicago, IL) .
  • the second member disk is laminated to the first member card or the first member in the form of jewelry; or the first member in the form of packaging for a book, video or audio cassette or packaging for a compact disk.
  • the compact disk, video cassette, book, gift, memorable or collectible article comprises subject and/or indicia identifying a biological source of the DNA of the device.
  • the device of the present invention also contemplates exhibiting or accomr bating the nucleic acid of a plurality of known individuals.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Dispositif pour la présentation d'un acide nucléique provenant d'une source biologique connue. Ce dispositif se présente de façon typique sous la forme d'une carte. L'acide nucléique provenant d'une source biologique connue est fixé à la carte. Des moyens de localisation sont prévus pour déterminer la position de l'acide nucléique sur la carte.
PCT/US1995/006324 1994-05-10 1995-05-10 Dispositif de presentation WO1995030749A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU25965/95A AU2596595A (en) 1994-05-10 1995-05-10 Exhibiting device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24016094A 1994-05-10 1994-05-10
US08/240,160 1994-05-10

Publications (1)

Publication Number Publication Date
WO1995030749A1 true WO1995030749A1 (fr) 1995-11-16

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Family Applications (1)

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PCT/US1995/006324 WO1995030749A1 (fr) 1994-05-10 1995-05-10 Dispositif de presentation

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AU (1) AU2596595A (fr)
WO (1) WO1995030749A1 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999067358A3 (fr) * 1998-06-24 2000-03-30 Stargene Co Ltd Procede relatif a l'elaboration d'objets renfermant de l'adn
WO2000010459A3 (fr) * 1998-08-25 2000-05-18 Gert Schlueter Dispositif et technique permettant de constituer et de conserver une banque personnelle de cellules, destinee notamment a des analyses genetiques par analyse de l'adn
WO2002049474A3 (fr) * 2000-12-21 2002-12-27 Alexander Olek Bijoux comportant une empreinte genetique
ES2204237A1 (es) * 2001-05-25 2004-04-16 Manuel Gonzalez Perez Recipiente para almacenamiento de material genetico.
EP1430462A4 (fr) * 2001-09-25 2006-12-20 Dnaform Kk Materiaux imprimes comprenant un support recouvert d'un oligomere et/ou d'un polymere, leur procede de preparation et leur procede de distribution et/ou de stockage
EP1997904A1 (fr) * 2007-05-29 2008-12-03 Identif GmbH Procédé destiné à l'identification infalsifiable d'une marque prévue sur un objet
CN102511979A (zh) * 2011-12-31 2012-06-27 福建师范大学 间接包被法制备储存人体基因信息的纪念物品的制备方法

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US5139812A (en) * 1989-07-07 1992-08-18 Bioprobe Systems Method and apparatus for high security crypto-marking for protecting valuable objects
US5360628A (en) * 1986-04-30 1994-11-01 Butland Trust Organization Technique for labeling an object for its identification and/or verification

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US5360628A (en) * 1986-04-30 1994-11-01 Butland Trust Organization Technique for labeling an object for its identification and/or verification
US5139812A (en) * 1989-07-07 1992-08-18 Bioprobe Systems Method and apparatus for high security crypto-marking for protecting valuable objects

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Title
INNIS et al., "PCR Protocols: A Guide to Methods and Applications", Published 1990, by ACADEMIC PRESS, INC., NEW YORK, NY, Chapter 13, by LEVENSON et al., "Nonisotopically Labeled Probes and Primers", pages 99-112. *
JOURNAL OF MOLECULAR BIOLOGY, Volume 98, issued 1975, E.M. SOUTHERN, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis", pages 503-517. *
PROC. NATL. ACAD. SCI. U.S.A., Volume 74, No. 12, issued December 1977, J.C. ALWINE et al., "Method For Detection of Specific RNAs in Agarose Gels by Transfer to Diazobenzyloxymethyl-Paper and Hybridization with DNA Probes", pages 5350-5354. *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999067358A3 (fr) * 1998-06-24 2000-03-30 Stargene Co Ltd Procede relatif a l'elaboration d'objets renfermant de l'adn
WO2000010459A3 (fr) * 1998-08-25 2000-05-18 Gert Schlueter Dispositif et technique permettant de constituer et de conserver une banque personnelle de cellules, destinee notamment a des analyses genetiques par analyse de l'adn
WO2002049474A3 (fr) * 2000-12-21 2002-12-27 Alexander Olek Bijoux comportant une empreinte genetique
ES2204237A1 (es) * 2001-05-25 2004-04-16 Manuel Gonzalez Perez Recipiente para almacenamiento de material genetico.
ES2326606A1 (es) * 2001-05-25 2009-10-15 Manuel Gonzalez Perez Recipiente para almacenamiento de material genetico y archivo historico de la familia.
ES2326606B1 (es) * 2001-05-25 2010-07-07 Manuel Gonzalez Perez Recipiente para almacenamiento de material genetico y archivo historico de la familia.
EP1430462A4 (fr) * 2001-09-25 2006-12-20 Dnaform Kk Materiaux imprimes comprenant un support recouvert d'un oligomere et/ou d'un polymere, leur procede de preparation et leur procede de distribution et/ou de stockage
EP1997904A1 (fr) * 2007-05-29 2008-12-03 Identif GmbH Procédé destiné à l'identification infalsifiable d'une marque prévue sur un objet
CN102511979A (zh) * 2011-12-31 2012-06-27 福建师范大学 间接包被法制备储存人体基因信息的纪念物品的制备方法

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