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WO1995021270A3 - Procede, reactif et kit pour la detection et l'amplification de sequences d'acides nucleiques - Google Patents

Procede, reactif et kit pour la detection et l'amplification de sequences d'acides nucleiques Download PDF

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Publication number
WO1995021270A3
WO1995021270A3 PCT/US1995/001468 US9501468W WO9521270A3 WO 1995021270 A3 WO1995021270 A3 WO 1995021270A3 US 9501468 W US9501468 W US 9501468W WO 9521270 A3 WO9521270 A3 WO 9521270A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
nucleic acid
amplification
kit
reagent
Prior art date
Application number
PCT/US1995/001468
Other languages
English (en)
Other versions
WO1995021270A9 (fr
WO1995021270A2 (fr
Inventor
Craig W Adams
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Priority to AU19113/95A priority Critical patent/AU1911395A/en
Publication of WO1995021270A2 publication Critical patent/WO1995021270A2/fr
Publication of WO1995021270A9 publication Critical patent/WO1995021270A9/fr
Publication of WO1995021270A3 publication Critical patent/WO1995021270A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention se rapporte à des procédés et à des réactifs qui servent à la détection et à l'amplification exponentielle de molécules d'acides nucléiques cibles. Ce procédé utilise généralement un oligonucléotide amorce qui s'hybride conjointement avec un oligonucléotide bloquant sur un brin de la molécule cible, et un oligonucléotide de fin de chaîne qui peut s'hybrider à l'oligonucléotide bloquant.
PCT/US1995/001468 1994-02-04 1995-02-03 Procede, reactif et kit pour la detection et l'amplification de sequences d'acides nucleiques WO1995021270A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU19113/95A AU1911395A (en) 1994-02-04 1995-02-03 Method, reagent and kit for the detection and amplification of nucleic acid sequences

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19363094A 1994-02-04 1994-02-04
US08/193,630 1994-02-04

Publications (3)

Publication Number Publication Date
WO1995021270A2 WO1995021270A2 (fr) 1995-08-10
WO1995021270A9 WO1995021270A9 (fr) 1995-09-28
WO1995021270A3 true WO1995021270A3 (fr) 1995-10-19

Family

ID=22714396

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/001468 WO1995021270A2 (fr) 1994-02-04 1995-02-03 Procede, reactif et kit pour la detection et l'amplification de sequences d'acides nucleiques

Country Status (2)

Country Link
AU (1) AU1911395A (fr)
WO (1) WO1995021270A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6180338B1 (en) * 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6489145B1 (en) 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0332435A2 (fr) * 1988-03-10 1989-09-13 Zeneca Limited Procédé pour détecter des séquences de nucléotides
WO1990001069A1 (fr) * 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Procede d'amplification et de detection de sequences d'acide nucleique
EP0357336A2 (fr) * 1988-08-25 1990-03-07 Syntex (U.S.A.) Inc. Méthode de détection de séquences spécifiques d'acide nucléique
GB2225112A (en) * 1988-11-22 1990-05-23 Ici Plc Hybridisation probes
WO1994003630A2 (fr) * 1992-08-04 1994-02-17 Beckman Instruments, Inc. Procede, reactif et trousse de detection et d'amplification de sequences d'acides nucleiques
WO1994017210A1 (fr) * 1993-01-27 1994-08-04 Oncor, Inc. Amplification de sequences d'acides nucleiques

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0332435A2 (fr) * 1988-03-10 1989-09-13 Zeneca Limited Procédé pour détecter des séquences de nucléotides
WO1990001069A1 (fr) * 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Procede d'amplification et de detection de sequences d'acide nucleique
EP0357336A2 (fr) * 1988-08-25 1990-03-07 Syntex (U.S.A.) Inc. Méthode de détection de séquences spécifiques d'acide nucléique
GB2225112A (en) * 1988-11-22 1990-05-23 Ici Plc Hybridisation probes
WO1994003630A2 (fr) * 1992-08-04 1994-02-17 Beckman Instruments, Inc. Procede, reactif et trousse de detection et d'amplification de sequences d'acides nucleiques
WO1994017210A1 (fr) * 1993-01-27 1994-08-04 Oncor, Inc. Amplification de sequences d'acides nucleiques

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
WRIGHT, P. ET AL.: "The polymerase chain reaction : Miracle or Mirrage", JOURNAL PATHOLOGY, vol. 162, pages 99 - 117 *
WU, D. ET AL.: "the ligation amplification reaction", GENOMICS, vol. 4, pages 560 - 69 *

Also Published As

Publication number Publication date
WO1995021270A2 (fr) 1995-08-10
AU1911395A (en) 1995-08-21

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