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WO1995006111A1 - Systeme mettant en ×uvre des reacteurs photobiologiques tubulaires dans la culture a l'echelle industrielle de micro-organismes photosynthetiques - Google Patents

Systeme mettant en ×uvre des reacteurs photobiologiques tubulaires dans la culture a l'echelle industrielle de micro-organismes photosynthetiques Download PDF

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Publication number
WO1995006111A1
WO1995006111A1 PCT/IT1994/000140 IT9400140W WO9506111A1 WO 1995006111 A1 WO1995006111 A1 WO 1995006111A1 IT 9400140 W IT9400140 W IT 9400140W WO 9506111 A1 WO9506111 A1 WO 9506111A1
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WO
WIPO (PCT)
Prior art keywords
tubes
culture
tube
bioreactor according
air
Prior art date
Application number
PCT/IT1994/000140
Other languages
English (en)
Inventor
Mario Roberto Tredici
Graziella Chini Zittelli
Silvia Biagiolini
Renato Carobbi
Francesco Favilli
Domenico Mannelli
Edoardo Pinzani
Original Assignee
Consiglio Nazionale Delle Ricerche
Inalco S.P.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consiglio Nazionale Delle Ricerche, Inalco S.P.A. filed Critical Consiglio Nazionale Delle Ricerche
Priority to AU75082/94A priority Critical patent/AU7508294A/en
Publication of WO1995006111A1 publication Critical patent/WO1995006111A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02—Percolation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers

Definitions

  • the invention relates to a bioreactor for the production of biomasses, especially of biomasses consisting of cultures of photosynthetic microorganisms or plant cells dispersed in nutrient-containing aqueous solutions, of the type that comprises at least one reaction tube or pipe that is essentially transparent to solar radiation.
  • the degree and seriousness of the phenomenon is inversely proportional to the diameter of the tubes used. With tubes having an internal diameter of 100 mm, degassing of the culture every 30-40 in is sufficient; 10-mm tubes require degassing every 3-4 min: this limits the length of tubular reactors of small diameters (20-30 mm) to a few hundred metres and significantly reduces the dimensions that can be achieved with the culture unit (module) . On the other hand, it is important to adopt small-diameter tubes for the sake of the economy of the process, as a higher culture density can be achieved with tubes of smaller diameter, and the costs of preparing and moving the medium are lower, besides which it is easier to prevent contamination by undesired microorganisms.
  • the invention relates to a bioreactor system for the production of biomasses, which system represents an improvement upon conventional systems.
  • means are provided for moving the biomass, that is the culture; a nozzle for gas is provided (at the starting end of said tube relative to the direction of movement) for blowing in gas (air or the like) which, as it moves through and/or with the biomass of the culture, provides mixing and removes the toxic gases generated by the photoreactions; also provided are means for returning the culture to the tube and means at the upper end of the tube for removing or optionally recovering the in- blown gas and the gas produced by the culture.
  • Said in- blown gas may be suitable for providing certain of the nutrient gases required by the cultured organisms (for example CO2 or N 2 ) •
  • reaction tube may actually be horizontal. In practice, and advantageously, it lies at a shallow angle to the horizontal.
  • a reaction tube may also be helical.
  • several reaction tubes are virtually straight and equidistant from each other.
  • a bioreactor comprises at least one reaction tube that is essentially transparent to solar radiation and lies at a shallow angle, generally not more than 10°, and especially of between 2° and 6°, to the horizontal, between two points at different levels; a nozzle for gas is provided at the lower end of the tube to blow in gas (air or the like) as a propellant to carry the culture upwards and to remove toxic gases generated by the photoreactions; also provided are means for returning the culture from the upper end to the lower end of the tube and means at the upper end of the tube for removing or optionally recovering the in-blown gas and the gas produced by the biomass of the culture.
  • gas air or the like
  • a plurality of reaction tubes are arranged in a preferably single- layer bundle, said tubes being approximately straight and being arranged side by side parallel with each other, preferably lying on an ''inclined geometrical plane; at least one of the ends of each of said tubes is connected to a collector; and means are provided for blowing in air at the base of at least some of said tubes.
  • the abovementioned means may comprise at least one air distributing pipe with nozzles for blowing air or other suitable gas into all or some of the reaction tubes.
  • Said collector may be connected to the lower ends of the reaction tubes, or to the upper ends of the reaction tubes.
  • the lower ends of the reaction tubes may be connected to a first or lower collector, and the upper ends of said tubes may be connected to a second or upper collector.
  • one or more return pipes for returning ths culture from the upper collector to the lower collector may be provided.
  • the system may comprise means for distributing, and nozzles for blowing in, air or other gas that are active alternately and selectively in some of said reaction tubes, while in other of said tubes the culture is being returned from the upper collector to the lower collector; the alternating of their actions periodically reverses the movement in each reaction tube.
  • said reaction tubes are arranged in two bundles with the tubes of each bundle being approximately parallel with each other; the tubes of said two bundles are angled in opposite directions from a lower middle area; air blowing means are provided in all or some of the tubes of each bundle, optionally in alternation.
  • collectors connecting together the reaction tubes at their upper ends are provided, and the tubes between the collectors form catenaries having average inclinations within the limits indicated relative to the horizontal; the air or gas is blown in at an intermediate lower point of each tube and in one of the ascending arms of at least some of the tubes, the in-blowing points active in adjacent tubes being located on opposite sides relative to the lowest point of the catenary. More particularly, in each tube there may be two opposing air-blowing nozzles on opposite sides of the lowest point of the catenary; and means are also provided for alternately supplying one or other of said blowing nozzles. Means may be provided for blowing air into one arm of one tube and into the opposing arm of the tubes oppositely adjacent thereto.
  • Another embodiment comprises a plurality of collectors at approximately the same level, between which run reaction tubes, all inclined- in the same direction, with joining pieces and with nozzles which blow in gas from distributing pipes; means are provided for returning the biomasses of the culture.
  • Said means may be formed by other reaction tubes inclined in the opposite direction to said reaction tubes, and having their own joining pieces and blowing nozzles.
  • the reaction tubes may be of synthetic resin or glass.
  • the reaction tubes are made - at least in groups - from two sheets of synthetic resin welded together to define the tubes and optionally also the collectors; in the latter case temperature- regulating water may be run over said sheets.
  • electrodes, probes and other sensors for monitoring the level and physical and chemical parameters of the culture may be placed in the upper collector.
  • devices such as filters and the like, to keep the environment inside the reactor ' sterile and to enable harvesting, introduction of culture medium, and other operations, to be performed in sterile conditions.
  • a system of the type defined above may comprise, in the collectors, shut-off members, which may be fully openable, for forcing the biomass of the culture to follow certain paths, especially in the case of a continuous-type operation.
  • efficient agitation and movement of the medium is achieved, as is efficient degassing that solves the problem of the accumulation of photosynthetic oxygen (or other gas that may be generaced during growth) .
  • This is achieved by blowing air into all or at least a large part of the reactor; the linear distance that can be achieved is at least an order of magnitude greater than is possible in conventional tubular photoreactors.
  • the invention does away with the need for pumps or other mechanical devices that damage the cultivated cells.
  • the use of blown air in order simultaneously to agitate and degas can be done advantageously by having the tubes of which the module is composed lie entirely or in large part at an angle to the horizontal.
  • the bubbles of air introduced at a slight overpressure into the lower sections also therefore exert a propulsive action as they rise to the top, moving and/or entraining the culture with them.
  • the air that has entrained the photosynthetic O2 is separated and removed. It may be discharged or reused, for example for aerobic processes which are activated in the presence of oxygen-enriched air.
  • Figs. 1 and 2 show in plan view and in section through the line II-II marked in Fig. 1, a schematic of an embodiment comprising two collectors;
  • Figs. 3 and 4 show, in a similar way to Figs. 1 and 2, a modified schematic for obtaining a cyclical alternation of the movement;
  • Figs. 5 and 6 show, in plan view and in a section taken through the line VI-VI marked in Fig. 5, a schematic of a system having reaction tubes following a catenary path with two slopes;
  • Fig. 7 shows, in a similar way to Fig. 6, a schematic similar to the previous schematic but having straight or virtually straight tubes;
  • Figs. 8 and 9 show two schematics similar to each other and suitable for systems to be installed on more or less level ground;
  • Figs. 10, 11 and 12 show a simplified schematic, in plan view and in vertical section, and a variant of the same in plan view;
  • Figs. 13, 14 and 15 show another simplified schematic, in plan view and in vertical section, and a variant of the same in plan view.
  • a plurality of tubes of transparent material 31 is set up on, for example, slightly inclined ground, in such a way that the tubes 31 lie at an angle to the horizontal of the order of, for example, between 1° and 10°, preferably between 2° and 6°; the tubes 31 may be oriented along the lines of maximum slope, or inclined relative to the lines of maximum slope and may be installed in contact with each other or separated from each other by a distance of, for example, the same order of magnitude as the diameter of the tubes.
  • the tubes 31 are connected to a lower collector 35; inside the lower collector 35 - or close to the collector - the ends of the tubes 31 are reached by nozzles 36 supplied with air from a distributing pipe 37 so that air is delivered to the interior of the tubes; it is possible simply to provide a pipe 37 which runs along the interior of the collector 35, passes through the lower portions of the tubes 31 that enter the collector, and has holes 36 by way of nozzles. At least one discharge 39 serves for emptying the reactor.
  • the tubes 31 are connected to an upper collector 43, in which there forms a free level of the liquid mass in an intermediate position; the upper collector 43 has outlets 43A for the air and other gases, and appropriate control systems such as probes, sensors of various kinds and thermometers.
  • the complex is filled with culture medium.
  • the air rises through the culture medium - in which the microorganisms or plant cells are developing - along the individual tubes 31 and creates a flow of liquid towards the upper collector 43; an equal quantity of liquid redescends down each tube 31 or preferably along return tubes or pipes 44 - arranged at intervals between the tubes 31 - which have no air-blowing nozzles and -may be of a different cross section from the tubes 31.
  • the return tubes 44 may be positioned in i.nilluminated areas (for example underneath the surface on which the tubes 31 are laid) .
  • the movements of liquid set up in the tubes 31 as the bubbles of air pass through it result in the removal of the toxic gases generated by the photosynthetic reactions, and also ensure an efficient, continuous changing of position of the liquid, and hence of the microbial cells.
  • the result is improved insolation and uniformity of the required reactions throughout the mass.
  • the tubes 31 and optionally 44 can be laid on the ground with the slope indicated above or greater slopes, but in the latter case the tubes are set at an angle relative to the lines of maximum slope so as to maintain the desired inclination.
  • nozzles 36A and 36B feeding into alternate tubes 31; in the case of two distributing pipes 37A and 37B, one of them will have nozzles feeding into tubes 31 in even-numbered positions and the other will have nozzles feeding into tubes in odd- numbered positions.
  • the nozzles and distributing pipes may be made according to the same * principles as those illustrated in Figs. 1 and 2.
  • air is supplied alternately and cyclically first to one, then to the other of the two distributing pipes 37A, 37B. It follows that in a cyclical manner, certain of the tubes 31 receive air and movements towards the collector 43 are set up in them, while in the other tubes 31 to which air is not fed, a return flow towards the collector 35 occurs.
  • Figs. 5 and 6 show an embodiment in which two groups of tubes 51 are arranged at equal intervals and with two opposing slopes from a lower middle area; two opposing tubes can be formed by a single catenary tube, the average inclinations being approximately as described above.
  • the tubes may be laid on natural opposing slopes or on suitably designed supports. At the two raised extremities the tubes 51 are connected up by respective collectors 59 and 61 having functions similar to those of the upper collector 43 of the previous exampl .
  • each of said two distributors 55 and 57 when active, blows air continuously into the ascending arm of tubes 51 in even-numbered positions and into the opposite ascending arm of tubes 51 in odd- numbered positions (or vice versa) ; it is thus possible to have reverse flows moving continuously in adjacent tubes 51.
  • the system can be discharged intermittently from the lower middle part of the individual tubes using a discharge collector 63, or from either or both of the collectors 59, 61 at one end, in a continuous manner, providing a supply of new medium at a distance from the culture discharge.
  • a discharge collector 63 or from either or both of the collectors 59, 61 at one end, in a continuous manner, providing a supply of new medium at a distance from the culture discharge.
  • the system can be made to operate on continuous cycle.
  • tubes 151 sloping at opposite angles run between a lower middle collector 153 and two upper outer collectors 159 and 161.
  • Distributors 155 and 157 can supply air to nozzles for blowing into the bottom end of the tubes 151 according to rules of cyclical selection similar to those discussed above in relation to previous examples.
  • suitable shut-off members which may be fully openable or adjustable - can be provided to impose certain obligatory paths on the mass undergoing treatment, where a continuous cycle is required, with culture medium being introduced at one end and product taken off at the other end of the system.
  • Figs. 8 and 9 show embodiments suitable for level ground and with structures of limited vertical height.
  • Fig. 8 shows a plurality of arrays of reaction tubes 71; the tubes 71 of each array lie with the slope already indicated between adjacent collectors 73, apart from an optional lower initial collector 731, to facilitate the return flow.
  • the tubes 71 have a joining piece 75 with an elbow from where the actual slope of the respective tube 71 commences, and in which there fits a nozzle 77 for blowing in air from a distributor 79, or with outlet holes directly from the distributor 79 (see Fig. 1) ; from the initial collector 731 in the low position, the tubes 71 lead directly upwards.
  • Connected to the initial collector 731 is at least one return pipe 81 arriving from the last 73X of the collectors 73. Operation is similar to that in the previous examples, with paths repeated and running over flat or approximately horizontal ground.
  • Fig. 9 in which the same reference numerals denote parts and components similar to those of Fig. 8 - the one or more return pipes 81 are now replaced by further arrays of tubes 171 - more or less intimately interposed between the tubes 71, for example in groups, in order to satisfy the demands for exposure to natural light - and these tubes 171 slope in the opposite direction to the tubes 71 and have similar vertical portions 175 and nozzles 177; this approach creates a return flow which can also be used for biosynthesis. Between the groups of tubes 71 and 171 it is possible to have suitable dividing walls in the collectors 73 and 73X.
  • collectors 73 may also be arranged vertically to correspond to the heights of the tubes connected to them, as indicated in broken lines at 273 in Fig. 8.
  • Simpler versions than those discussed thus far may adopt a single collector, lower or upper, with air blowing means for the purposes already indicated, and with tubes sloping at a shallow angle to the horizontal.
  • Figs. 10 and 11 show an arrangement with a single upper collector 201, from which run tubes 203 closed at the lower end and traversed by a distributing pipe 205 with nozzles 207 for blowing air into the tubes 203. Though rather slowly, the biomass does still move along the tubes 203 and the toxic gases generated inside them are still removed. In the variant shown in Fig.
  • an air distributing pipe 305 or a pair of' alternately operating air distributing pipes 305A have the same function as 207 and 207A.
  • protective filters and other means for ensuring sterility and/or avoiding contamination of the interior and/or exterior can be provided.
  • 125 tubes having an external diameter of 40 mm, an internal diameter of 30 mm and lengths of 200 m may be laid out parallel on an inclined surface with an interval of one diameter from one to the next.
  • the tubes are connected at the upper and/or lower ends. Compressed air is blown into the bottom ends of alternate tubes (or tube sections) in order to get the culture moving and mixing, with a cyclical return of the mass to the lower end.
  • a culture unit having a surface area of 2000 m 2 (10 200 m) and approximately 18,000 litres of useful volume is constructed.
  • Tubes made of PVC, polyethylene, glass, polymethyl methacrylate, polycarbonate or other transparent material can be used.
  • the diameter of the tubes may vary between 15 and 50 mm, but other diameters are possible, as also are structures made of polyethylene or PVC films welded to form tubes connected together on the same principles.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
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  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Bioréacteur destiné à la production de cultures de micro-organismes photosynthétiques ou de cellules végétales dispersées dans des solutions aqueuses renfermant des substances nutritives. Ce réacteur comporte des tubes réactionnels (31) essentiellement transparents vis-à-vis du rayonnement solaire et formant un angle très faible, généralment compris entre 2° et 6°, avec l'horizontale, lesdits tubes s'étendant entre deux capteurs (35, 43) situés à des hauteurs différentes. Une buse (36A, 36B) est prévue à l'extrémité inférieure de chaque tube (31) et destinée à insuffler en alternance de l'air (ou analogue) servant de gaz véhicule portant la culture vers le haut et évacuant les gaz toxiques engendrés par les photoréactions. Certains tubes (31), agissant en alternance, renvoient la culture du capteur supérieur (43) vers le capteur inférieur (35), et le gaz insufflé ou produit par la culture est évacué ou éventuellement récupéré au niveau du capteur supérieur (43).
PCT/IT1994/000140 1993-08-27 1994-08-25 Systeme mettant en ×uvre des reacteurs photobiologiques tubulaires dans la culture a l'echelle industrielle de micro-organismes photosynthetiques WO1995006111A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU75082/94A AU7508294A (en) 1993-08-27 1994-08-25 System using tubular photobioreactors for the industrial culture of photosynthetic microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITFI93A000167 1993-08-27
ITFI930167A IT1262502B (it) 1993-08-27 1993-08-27 Impianto a fotobioreattori tubolari per la coltura industriale di microrganismi fotosintetici.

Publications (1)

Publication Number Publication Date
WO1995006111A1 true WO1995006111A1 (fr) 1995-03-02

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PCT/IT1994/000140 WO1995006111A1 (fr) 1993-08-27 1994-08-25 Systeme mettant en ×uvre des reacteurs photobiologiques tubulaires dans la culture a l'echelle industrielle de micro-organismes photosynthetiques

Country Status (7)

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AU (1) AU7508294A (fr)
IL (1) IL110723A0 (fr)
IT (1) IT1262502B (fr)
MA (1) MA23313A1 (fr)
TN (1) TNSN94087A1 (fr)
WO (1) WO1995006111A1 (fr)
ZA (1) ZA945818B (fr)

Cited By (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2320031A (en) * 1996-12-06 1998-06-10 Stephen Skill Apparatus for the culture of microrganisms in the presence of light
DE19747994C1 (de) * 1997-10-17 1999-01-28 Torsten Steinau Verfahrenstech Bioreaktor mit U-förmigen Reaktorelementen
WO1999015620A1 (fr) * 1997-09-19 1999-04-01 Biotechna Environmental International Limited Bioreacteur modifie
GB2330589A (en) * 1997-10-22 1999-04-28 Stephen Skill Bioreactor for the culture of organisms
GB2331762A (en) * 1997-09-19 1999-06-02 Biotechna Environmental Intern Tubular bioreactor
GB2335199A (en) * 1998-03-11 1999-09-15 Applied Photosynthetics Limite Photobioreactor apparatus
DE10009060A1 (de) * 2000-02-25 2001-09-06 Dlr Ev Solarer Photoreaktor
WO2008010737A1 (fr) * 2006-07-21 2008-01-24 Tecnia Processos E Equipamentos Industriais E Ambintais Photobioréacteur pour une culture de microorganismes photosynthétiques
ES2319376A1 (es) * 2008-11-10 2009-05-06 Juan Luis Ripolles Romeu "fotobiorreactor".
DE102009013936A1 (de) 2009-03-19 2010-09-23 Wilfried Rosendahl Solarer Photoreaktor
WO2010150190A3 (fr) * 2009-06-23 2011-04-07 University Of The Witwatersrand, Johannesburg Système de culture d'algue diphasique
US7980024B2 (en) 2007-04-27 2011-07-19 Algae Systems, Inc. Photobioreactor systems positioned on bodies of water
WO2011143619A1 (fr) * 2010-05-14 2011-11-17 Photon8, Inc. Système et procédé de création d'une onde de déplacement à l'intérieur d'un réacteur photobiotique pour accroître la croissance des algues
US8110395B2 (en) 2006-07-10 2012-02-07 Algae Systems, LLC Photobioreactor systems and methods for treating CO2-enriched gas and producing biomass
US20120164712A1 (en) * 2009-06-10 2012-06-28 Gary Kenneth Ellem Production of algae
US20120252112A1 (en) * 2009-10-01 2012-10-04 Jeremy Pruvost Thin-layer photobioreactor with high volume productivity
ITPG20110019A1 (it) * 2011-09-06 2013-03-07 Davide Di Giuseppe Stefanori Bioreattore per la produzione di microrganismi e relativo metodo
US8507253B2 (en) 2002-05-13 2013-08-13 Algae Systems, LLC Photobioreactor cell culture systems, methods for preconditioning photosynthetic organisms, and cultures of photosynthetic organisms produced thereby
US8709808B2 (en) 2009-11-19 2014-04-29 The Arizona Board Of Regents Accordion bioreactor
WO2014133793A1 (fr) 2013-02-26 2014-09-04 Heliae Development, Llc Bioréacteur tubulaire modulaire
AU2010346632B2 (en) * 2010-02-23 2014-09-25 Margalef, Pere Method and apparatus for providing a photobioreactor
EP2496684A4 (fr) * 2009-11-02 2015-04-15 William R Kassebaum Système de photobioréacteur et son procédé d'utilisation
EP2748114A4 (fr) * 2011-10-14 2015-07-01 Presby Patent Trust Appareil de traitement de déchets liquides
NO20141197A1 (no) * 2014-10-07 2016-01-04 Lars Aglen Reaktor for enzymatisk hydrolyse av et råmateriale
CN107109326A (zh) * 2014-10-07 2017-08-29 N&U公共有限责任公司 用于酶处理的紧凑反应器
US10829725B2 (en) 2014-02-01 2020-11-10 The Arizona Board Of Regents On Behalf Of The University Of Arizona Air accordion bioreactor

Citations (4)

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US3468057A (en) * 1966-06-01 1969-09-23 Inst Francais Du Petrole Process for the culture of algae and apparatus therefor
GB2118572A (en) * 1982-03-27 1983-11-02 Queen Elizabeth College Culture growth and apparatus therefor
FR2596412A1 (fr) * 1986-03-26 1987-10-02 Commissariat Energie Atomique Photobioreacteur
FR2685344A1 (fr) * 1991-12-18 1993-06-25 Commissariat Energie Atomique Dispositif de production intensive et controlee de micro-organismes photosynthetiques fragiles.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3468057A (en) * 1966-06-01 1969-09-23 Inst Francais Du Petrole Process for the culture of algae and apparatus therefor
GB2118572A (en) * 1982-03-27 1983-11-02 Queen Elizabeth College Culture growth and apparatus therefor
FR2596412A1 (fr) * 1986-03-26 1987-10-02 Commissariat Energie Atomique Photobioreacteur
FR2685344A1 (fr) * 1991-12-18 1993-06-25 Commissariat Energie Atomique Dispositif de production intensive et controlee de micro-organismes photosynthetiques fragiles.

Cited By (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2320031B (en) * 1996-12-06 2001-05-02 Stephen Skill Apparatus and method for growing culture of micro-organisms
WO1998024879A1 (fr) * 1996-12-06 1998-06-11 Photosynthesis (Jersey) Limited Culture de micro-organismes
GB2320031A (en) * 1996-12-06 1998-06-10 Stephen Skill Apparatus for the culture of microrganisms in the presence of light
AU736564B2 (en) * 1996-12-06 2001-08-02 Photosynthesis (Jersey) Limited Culture of micro-organisms
WO1999015620A1 (fr) * 1997-09-19 1999-04-01 Biotechna Environmental International Limited Bioreacteur modifie
GB2331762A (en) * 1997-09-19 1999-06-02 Biotechna Environmental Intern Tubular bioreactor
US6174720B1 (en) * 1997-09-19 2001-01-16 Biotechna Environmental International Limited Modified bioreactor
AU731796B2 (en) * 1997-09-19 2001-04-05 Biotechna Environmental International Limited Modified bioreactor
DE19747994C1 (de) * 1997-10-17 1999-01-28 Torsten Steinau Verfahrenstech Bioreaktor mit U-förmigen Reaktorelementen
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TNSN94087A1 (fr) 1995-04-25
AU7508294A (en) 1995-03-21
IT1262502B (it) 1996-06-28
ITFI930167A0 (it) 1993-08-27
ITFI930167A1 (it) 1995-02-27
MA23313A1 (fr) 1995-04-01
IL110723A0 (en) 1994-11-11

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