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WO1994026883A3 - Procede de production d'enzyme exempte de poussiere - Google Patents

Procede de production d'enzyme exempte de poussiere Download PDF

Info

Publication number
WO1994026883A3
WO1994026883A3 PCT/EP1994/001642 EP9401642W WO9426883A3 WO 1994026883 A3 WO1994026883 A3 WO 1994026883A3 EP 9401642 W EP9401642 W EP 9401642W WO 9426883 A3 WO9426883 A3 WO 9426883A3
Authority
WO
WIPO (PCT)
Prior art keywords
granules
enzyme
dust
pore size
free enzyme
Prior art date
Application number
PCT/EP1994/001642
Other languages
English (en)
Other versions
WO1994026883A9 (fr
WO1994026883A2 (fr
Inventor
Carl Sidonius Maria Andela
Jan Feijen
Marc Dillissen
Original Assignee
Gist Brocades Nv
Carl Sidonius Maria Andela
Jan Feijen
Marc Dillissen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv, Carl Sidonius Maria Andela, Jan Feijen, Marc Dillissen filed Critical Gist Brocades Nv
Priority to JP6525000A priority Critical patent/JPH07509141A/ja
Priority to AU69705/94A priority patent/AU677342B2/en
Priority to PL94307854A priority patent/PL307854A1/xx
Priority to EP94918349A priority patent/EP0675952A1/fr
Priority to KR1019950700195A priority patent/KR950702625A/ko
Publication of WO1994026883A2 publication Critical patent/WO1994026883A2/fr
Publication of WO1994026883A9 publication Critical patent/WO1994026883A9/fr
Priority to FI950183A priority patent/FI950183A0/fi
Publication of WO1994026883A3 publication Critical patent/WO1994026883A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38672—Granulated or coated enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/98—Preparation of granular or free-flowing enzyme compositions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Inorganic Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicinal Preparation (AREA)
  • Glanulating (AREA)
  • Confectionery (AREA)

Abstract

Selon l'invention, on obtient de nouvelles granules d'enzyme présntant de propriétés améliorées. L'élément de base desdites granules est un noyau constitué d'une particule présentant une taille de pore et une distribution de la taille des pores telles qu'elles permettent à une solution enzymatique de pénétrer dans ladite particule. Ainsi, le matériau constituant le noyau comprend l'enzyme sous forme liquide, ce qui élimine l'inconvénient d'avoir à traiter les enzymes sous forme de poudre. De plus, on obtient une bonne aptitude à l'écoulement et au dosage. Les granules contenant l'enzyme sont de préférence revêtues d'un matériau macromoléculaire filmogène. Ces granules ont une faible teneur en poussière et leur résistance mécanique est améliorée. L'invention concerne également un procédé permettant de préparer ces granules d'enzyme.
PCT/EP1994/001642 1993-05-18 1994-05-18 Procede de production d'enzyme exempte de poussiere WO1994026883A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP6525000A JPH07509141A (ja) 1993-05-18 1994-05-18 細塵を含まない酵素の製造方法
AU69705/94A AU677342B2 (en) 1993-05-18 1994-05-18 Process for dust-free enzyme manufacture
PL94307854A PL307854A1 (en) 1993-05-18 1994-05-18 Method of obtaining dust-free enzyme
EP94918349A EP0675952A1 (fr) 1993-05-18 1994-05-18 Procede de production d'enzyme exempte de poussiere
KR1019950700195A KR950702625A (ko) 1993-05-18 1994-05-18 비살포성 효소의 제조방법(process for dust-free enzyme manufacture)
FI950183A FI950183A0 (fi) 1993-05-18 1995-01-16 Menetelmä pölyämättömän entsyymin valmistamiseksi

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL93201428.5 1993-05-18
EP93201428 1993-05-18

Publications (3)

Publication Number Publication Date
WO1994026883A2 WO1994026883A2 (fr) 1994-11-24
WO1994026883A9 WO1994026883A9 (fr) 1995-01-05
WO1994026883A3 true WO1994026883A3 (fr) 1995-03-02

Family

ID=8213835

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1994/001642 WO1994026883A2 (fr) 1993-05-18 1994-05-18 Procede de production d'enzyme exempte de poussiere

Country Status (8)

Country Link
EP (1) EP0675952A1 (fr)
KR (1) KR950702625A (fr)
CN (1) CN1110059A (fr)
AU (1) AU677342B2 (fr)
CA (1) CA2140530A1 (fr)
FI (1) FI950183A0 (fr)
PL (1) PL307854A1 (fr)
WO (1) WO1994026883A2 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7371423B2 (en) 1997-04-09 2008-05-13 Danisco, A/S Method for preparing flour doughs and products made from such doughs using lipase
US7622290B2 (en) 2004-03-12 2009-11-24 Danisco A/S Fungal lipolytic enzymes, nucleic acids encoding, and uses thereof
US7638293B2 (en) 2003-01-17 2009-12-29 Danisco A/S Method
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
USRE43341E1 (en) 1995-06-07 2012-05-01 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US8889371B2 (en) 2004-07-16 2014-11-18 Dupont Nutrition Biosciences Aps Lipolytic enzyme: uses thereof in the food industry

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022606A1 (fr) * 1994-02-21 1995-08-24 Novo Nordisk A/S Procede pour la production d'une preparation enzymatique immobilisee et utilisation de cette preparation
DE69605009T2 (de) * 1995-05-29 2000-05-25 Kao Corp., Tokio/Tokyo Enzymhaltiges granulat und verfahren zu seiner herstellung
AR006643A1 (es) 1996-04-12 1999-09-08 Novozymes As Granulos con contenido enzimatico, un proceso para su preparacion, una composicion detergente que los comprende y el uso de los mismos.
TW409035B (en) 1997-06-04 2000-10-21 Gist Brocades Bv Starch-based enzyme granulates
EP0913468A3 (fr) * 1997-07-22 2001-06-13 Dsm N.V. Composition pour l'amélioration du pain
CA2243769A1 (fr) * 1997-07-22 1999-01-22 Gist-Brocades B.V. Nouvelle composition ameliorant le pain
DK1042458T3 (da) * 1997-12-23 2004-03-22 Novozymes As Fremgangsmåde til immobilisering af enzymer
US6156548A (en) 1997-12-23 2000-12-05 Novo Nordisk A/S Immobilization of enzymes with a fluidized bed for use in an organic medium
GB2343191A (en) * 1998-10-30 2000-05-03 Procter & Gamble Low dusting components for detergent compositions
US6630436B1 (en) 1998-10-30 2003-10-07 The Procter & Gamble Company Impact resistant solid component
ES2220437T3 (es) 1999-02-10 2004-12-16 Basf Aktiengesellschaft Granulados que contienen enzimas para piensos.
DE19929257A1 (de) * 1999-06-25 2000-12-28 Basf Ag Polymerbeschichtete, granulierte enzymhaltige Futtermittelzusätze und Verfahren zu deren Herstellung
DE60328357D1 (de) 2002-01-15 2009-08-27 Basf Se Enzymhaltige futtergranulate
DK1467630T4 (da) 2002-01-15 2011-07-25 Basf Se Granulater, der indeholder foderenzymer
DE102006053071A1 (de) * 2006-11-10 2008-05-15 Ab Enzymes Gmbh Protein-enthaltender Stoff mit erhöhter Temperaturstabilität
US9560877B2 (en) 2012-03-13 2017-02-07 General Mills, Inc. Cereal-based product with improved eating quality fortified with dietary fiber and/or calcium
WO2019076834A1 (fr) * 2017-10-16 2019-04-25 Novozymes A/S Granulés à faible teneur en poussière

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0193829A2 (fr) * 1985-03-05 1986-09-10 SOLVAY ENZYMES, INC. (a Delaware corporation) Formulation d'enzyme particulaire et non poussiéreuse
EP0206418A2 (fr) * 1985-06-28 1986-12-30 The Procter & Gamble Company Composition granulaire contenant un agent de blanchiment sec et une enzyme stable
EP0430395A1 (fr) * 1989-11-24 1991-06-05 Kingston Diagnostics, L.P. Enzymes stabilisées dispersées
US5177013A (en) * 1989-07-31 1993-01-05 Ajinomoto Co., Inc. Preparation of an immobilized lipase having a low water content without drying
EP0542351A1 (fr) * 1991-11-11 1993-05-19 Akzo Nobel N.V. Procédé pour la préparation des granules de sel

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1435905A (en) * 1972-11-03 1976-05-19 Unilever Ltd Enzyme granules
GB1590432A (en) * 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
FR2446317A1 (fr) * 1979-01-12 1980-08-08 Solvay Granules complexes contenant des substances proteiniques actives et procedes pour leur fabrication, leur utilisation, et leur regeneration
ES2167319T3 (es) * 1991-10-07 2002-05-16 Genencor Int Granulo que contiene una enzima revestida.

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0193829A2 (fr) * 1985-03-05 1986-09-10 SOLVAY ENZYMES, INC. (a Delaware corporation) Formulation d'enzyme particulaire et non poussiéreuse
EP0206418A2 (fr) * 1985-06-28 1986-12-30 The Procter & Gamble Company Composition granulaire contenant un agent de blanchiment sec et une enzyme stable
US5177013A (en) * 1989-07-31 1993-01-05 Ajinomoto Co., Inc. Preparation of an immobilized lipase having a low water content without drying
EP0430395A1 (fr) * 1989-11-24 1991-06-05 Kingston Diagnostics, L.P. Enzymes stabilisées dispersées
EP0542351A1 (fr) * 1991-11-11 1993-05-19 Akzo Nobel N.V. Procédé pour la préparation des granules de sel

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43341E1 (en) 1995-06-07 2012-05-01 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US7371423B2 (en) 1997-04-09 2008-05-13 Danisco, A/S Method for preparing flour doughs and products made from such doughs using lipase
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
US7638293B2 (en) 2003-01-17 2009-12-29 Danisco A/S Method
US7622290B2 (en) 2004-03-12 2009-11-24 Danisco A/S Fungal lipolytic enzymes, nucleic acids encoding, and uses thereof
US8889371B2 (en) 2004-07-16 2014-11-18 Dupont Nutrition Biosciences Aps Lipolytic enzyme: uses thereof in the food industry

Also Published As

Publication number Publication date
AU6970594A (en) 1994-12-12
CN1110059A (zh) 1995-10-11
AU677342B2 (en) 1997-04-17
CA2140530A1 (fr) 1994-11-24
FI950183L (fi) 1995-01-16
EP0675952A1 (fr) 1995-10-11
WO1994026883A2 (fr) 1994-11-24
FI950183A0 (fi) 1995-01-16
KR950702625A (ko) 1995-07-29
PL307854A1 (en) 1995-06-26

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