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WO1994019929A2 - Graines de brassica ayant des niveaux reduits de glucosinolates - Google Patents

Graines de brassica ayant des niveaux reduits de glucosinolates Download PDF

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Publication number
WO1994019929A2
WO1994019929A2 PCT/US1994/001869 US9401869W WO9419929A2 WO 1994019929 A2 WO1994019929 A2 WO 1994019929A2 US 9401869 W US9401869 W US 9401869W WO 9419929 A2 WO9419929 A2 WO 9419929A2
Authority
WO
WIPO (PCT)
Prior art keywords
glucosinolate
seed
glucosinolates
meal
seeds
Prior art date
Application number
PCT/US1994/001869
Other languages
English (en)
Other versions
WO1994019929A3 (fr
Inventor
Lorin Roger Debonte
Zhegong Fan
Original Assignee
Cargill, Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cargill, Incorporated filed Critical Cargill, Incorporated
Priority to AT94910149T priority Critical patent/ATE192016T1/de
Priority to AU62703/94A priority patent/AU696492B2/en
Priority to DK94910149T priority patent/DK0685993T3/da
Priority to US08/507,394 priority patent/US5866762A/en
Priority to CA002157019A priority patent/CA2157019C/fr
Priority to DE69424155T priority patent/DE69424155T2/de
Priority to EP94910149A priority patent/EP0685993B1/fr
Publication of WO1994019929A2 publication Critical patent/WO1994019929A2/fr
Publication of WO1994019929A3 publication Critical patent/WO1994019929A3/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A01H1/101—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds

Definitions

  • the present invention relates to Brassica seeds, meal, plant lines and progeny thereof having a reduced level of glucosinolates.
  • Canola meal is widely employed as a protein supplement in animal feed.
  • the feeding value of canola meal is reduced due to the anti-nutritive effects of the breakdown products of the glucosinolates, which reduce feed intake and growth in non-ruminant animals.
  • Glucosinolates in the seed are broken down during the extraction process by the enzyme myrosinase to form isothiocyanates and nitriles. These breakdown products may also inhibit thyroid function, leading to goiter.
  • the feed value of canola meal can be improved by reducing or eliminating glucosinolates from canola seeds.
  • Typical glucosinolate levels in canola meal and seed are disclosed in the following references: 1) Shahidi et al. Journal of Food Quality, 11, 421-431 (1989), and 2) Lichter et al., Plant Breeding 100, 209-221 (1988) and 3) Kraling et al., Plant Breeding, 105, 33-39 (1990).
  • the typical range for the glucosinolates content of conventional B. napus double low canola, varieties in mmol/g of seed at 40% oil content and 8.5% moisture is as follows:
  • the present invention provides B. napus lines, seeds, and meal having a reduced level of glucosinolates. SUMMARY OF INVENTION
  • the present invention comprises a seed comprising a.
  • Brassica napus canola variety having a maximum content of glucosinolates of about 3.4 mmol/g seed and belonging to a line in which said glucosinolate content has been stabilized for both the generation to which the seed belongs and its parent generation and progeny thereof.
  • the present invention further comprises a plant line comprising a Brassica napus canola variety which produces seeds having a maximum content of glucosinolates of about 3.4 mmol/g seed and in which said glucosinolate content is stabilized for both the generation to which the seed belongs and its parent generation.
  • the present invention further comprises a canola meal derived from the above-described seeds.
  • This canola meal has a maximum content of glucosinolates of 5.7 mmol/g of oil free meal.
  • the present invention provides seeds, meal and plant lines having a reduced level of glucosinolates generated by creating specific mutations in the glucosinolate biosynthetic pathway.
  • Scheme I depicts the biosynthetic pathway of major aliphatic glucosinolates in Brassica.
  • a "line” is a group of plants that display little or no genetic variation between individuals for at least one trait. Such lines may be created by several generations of self-pollination and selection, or vegetative propagation from a single parent using tissue or cell culture techniques. As used herein, the term “variety” refers to a line which is used for commercial production.
  • mutation refers to a detectable and heritable genetic change not caused by segregation or genetic recombination.
  • “Mutant” refers to an individual, or lineage of individuals, possessing a genetic mutation.
  • the term “Mutagenesis” refers to the use of a mutagenic agent to induce random genetic mutations within a population of individuals. The treated population, or a subsequent generation of that population, is then screened for usable trait(s) that result from the mutations. A “population” is any group of individuals that share a common gene pool. As used herein "M Q " is untreated seed.
  • Mi is the seed (and resulting plants) exposed to a mutagenic agent
  • M2 is the progeny (seeds and plants) of self-pollinated M A plants
  • M3 is the progeny of self-pollinated M2 plants
  • M4 is the progeny of self-pollinated M3 plants.
  • M 5 is the progeny of self-pollinated M4 plants.
  • Mg is the progeny of self-pollinated plants of the previous generation.
  • progeny as used herein means the plants and seeds of all subsequent generations resulting from a particular designated generation.
  • Stability or “stable” as used herein means that with respect to a given component, the component is maintained from generation to generation for at least two generations and preferably at least three generations at substantially the same level, e.g., preferably ⁇ 15%, more preferably ⁇ 10%, most preferably ⁇ 5%.
  • the method of invention is capable of creating lines with improved glucosinolate compositions stable up to ⁇ 5% from generation to generation.
  • the above stability may be affected by temperature, location, soil fertility, stress and time of planting. Thus, comparison of glucosinolate profiles should be made from seeds produced under similar growing conditions. Stability may be measured based on knowledge of prior generation.
  • Brassica plants whose seed oil contains less than 2% erucic acid.
  • the same varieties have also been bred so that the defatted meal contains less than 30 mmol glucosinolates/gram.
  • "Canola seed” is referred to herein as the seed of the genus Brassica which shall contain less than 30 mmoles of total glucosinolates per gram of whole seed at a moisture content of 8.5%; and the oil component of such seed shall contain less than 2% of all fatty acids as erucic acid.
  • canola meal is used herein to describe a protein meal derived from seeds of the genus Brassica containing less than 30 mmoles of total glucosinolates per gram of defatted meal at a moisture content of 8.5%.
  • canola oil is used herein to describe an oil derived from the seed of the genus Brassica, with less than 2% of all fatty acids as erucic acid.
  • the glucosinolate contents for the present invention are reported as mmol/gm seed at 40% oil content and at a moisture content of 8.5%.
  • the reported values to older references mmol/gm of seed can be converted to mmol/gm of oil free meal, at 8.5% moisture using the following conversion: (mmol/gm seed)/(l .0 - 0.4).
  • a comparison of this conversion from mmol of glucosinolates/gm of seed to mmol of glucosinolates/gm of defatted meal at 8.5% moisture using field grown IMC 129 seed is presented in Table V in Example 1 hereinafter.
  • IMC 129 seeds (M Q ) were mutagenized with N-methyl-N'-nitro-N- nitrosoguanidine (MNNG).
  • MNNG N-methyl-N'-nitro-N- nitrosoguanidine
  • IMC 129 is a canola quality commercial Spring variety with high oleic acid grown by InterMountain Canola Inc. in the northwestern United States registered under U.S. Plant Variety Protection Certificate 9100151.
  • the glucosinolate composition of field-grown IMC 129 has remained stable under commercial production.
  • the glucosinolate composition as mmoles per gram of whole seed at a moisture content of 8.5% and 40% oil content is as follows: 8.77 of total glucosinolates, 3.97 of 2-hydroxy-3-butenyl glucosinolate, 0.38 of allyl glucosinolate, 0.35 of 2-hydroxy-4-pentyl glucosinolate, 1.84 of 3-butenyl glucosinolate, 1.66 of 4-hydroxy-3-indolyl methyl glucosinolate, 0.22 of 4-pentyl glucosinolate, and 0.32 of 3-indolylmethyl glucosinolate.
  • the disclosed method may be applied to all oilseed Brassica species, and to both Spring and Winter maturing types within each species.
  • Physical mutagens including but not limited to X-rays, UV rays, and other physical treatments which cause chromosome damage, and other chemical mutagens, including but not limited to ethidium bromide, nitrosoguanidine, diepoxybutane etc. may also be used to induce mutations.
  • the mutagenesis treatment may also be applied to other stages of plant development, including but not limited to cell cultures, embryos, microspores and shoot spices. Once the mutation has been identified it can be transferred into other B. napus varieties by cross-pollination.
  • the present invention includes such cross-pollinated species.
  • the Mj seeds derived from the mutagenesis treatment were planted in the greenhouse and Mj plants were individually self-pollinated.
  • M2 seed was harvested and planted in the greenhouse, and individually self-pollinated to advance to the next generation.
  • M3 seeds were screened for total glucosinolate content using a TruBluGlu meter. This meter is available from Dr. R. J. W. Truscott at Systrix Pty. Ltd., University of Wollongong, P.O. Box 1144, Wollongong, N.S .W. 25000, Australia.
  • the analytical procedure employed is detailed in Truscott et al., Proceedings of the Eighth International Rapeseed Congress 1991, McGregor, D., Ed., Vol. 5, pp. 1425-1427.
  • Example 1 M4 seeds were analyzed by high pressure liquid chromatography to determine specific alterations in the glucosinolate composition. Those lines which remained stable in glucosinolate content were regarded as stable mutations.
  • M4 seeds were evaluated for mutations on the basis of a Z-distribution.
  • An extremely stringent 1 in 10,000 rejection rate was employed to establish statistical thresholds to distinguish mutation events from existing variation.
  • Mean and standard deviation values were determined from the non-mutagenized IMC 129 control population.
  • the upper and lower statistical thresholds for each glucosinolate were determined from the mean value of the population ⁇ the standard deviation, multiplied by the Z-distribution. Based on a population size of 10,000, the confidence interval is 99.99%.
  • Stable mutations as used herein are defined as M3 or more advanced lines which maintain a selected altered glucosinolate profile for a minimum of three generations, and exceeding established statistical thresholds for a minimum of two generations, as determined by liquid chromatographic analysis of a minimum of 200 mg (approximately 50 seeds) of randomly selected seeds. Alternatively, stability may be measured in the same way by comparing to subsequent generations. In subsequent generations, stability is defined as having similar glucosinolate profiles in the seed as that of the prior or subsequent generation when grown under substantially similar conditions.
  • Stable mutants having reduced levels of glucosinolates were obtained. Seeds and plant lines producing such seeds, having a maximum glucosinolate content of 3.4 mmoles per g seed were obtained. Generally, the total glucosinolate as mmol/g of seed ranged from 2.01 to 3.41. The maximum content and range for the individual glucosinolates is as follows as mmol/g seed at 8.5% moisture and 40% oil content:
  • the glucosinolate content of the canola meal derived from the seeds of these stable mutants was calculated as previously described from the seed glucosinolate content.
  • the meal has a maximum total glucosinolate content of 5.7 mmol/g on an oil free basis at 8.5% moisture. Generally, the total glucosinolate content ranged from 3.4 to 5.7 mmol/g.
  • the individual glucosinolate levels are as follows in mmol/g of oil free meal at 8.5% moisture.
  • IMC 129 seeds (M Q ) were mutagenized with N-methyl-N'-nitro-N- nitrosoguanidine (MNNG).
  • MNNG N-methyl-N'-nitro-N- nitrosoguanidine
  • IMC 129 is a canola quality commercial Spring variety with high oleic acid grown by InterMountain Canola Inc. in the northwestern United States.
  • the glucosinolate composition of field-grown IMC 129 is as described above and has remained stable under commercial production.
  • Prior to mutagenesis 45,000 seeds of IMC 129 were preimbibed in 300 seed lots for two hours on wet filter paper saturated in 0.05 M Sorenson's buffer (pH 6.1). The preimbibed seeds were placed in 1.0 mM MNNG for three hours.
  • the M2 seed from individual plants were catalogued and stored. Approximately 9,500 individual M2 lines were sown in the greenhouse in 4 inch pots containing Pro-Mix soil. The plants were maintained at 25°C/15°C,
  • the M3 seed was screened using the TruBluGlu meter available from Dr. R. J. W. Truscott at Systrix Pty, Ltd., University of Wollongong, P.O. Box
  • the means and standard deviations were calculated from an external control population of non-mutagenized IMC 129 grown next to the mutagenized lines.
  • Statistical thresholds were established for the glucosinolates using the control populations.
  • the lower thresholds were determined to be 3.6 standard deviations below the IMC 129 control means with a 99.99% confidence interval.
  • the lower thresholds used in the greenhouse selection are listed in Table IV. Selected M3 lines with altered glucosinolate compositions were planted in the greenhouse to advance the next generation.
  • Table I summarizes the reduced level of glucosinolates for the mutant lines generated. Significant reductions in the levels of 2-hydroxy-3-butenyl glucosinolate, 3-butenyl glucosinolate, and 4-hydroxy-3-indolylmethyl glucosinolate contributed to the overall low levels of total glucosinolates.
  • Table I presents the resulting overall glucosinolate compositions for the mutant lines of the present invention.
  • Table II presents the resulting glucosinolate compositions of mutants having low 2-hydroxy-3-butenyl glucosinolate for several indicated lines.
  • Table El presents the glucosinolate compositions of selected mutants with low 4-hydroxy-3-indolylmethyl glucosinolate for the lines indicated.
  • Table IV illustrates the statistical thresholds employed for selection of the low glucosinolate mutations.
  • Table V presents glucosinolate values for the control IMC 129. All values represent mmoles glucosinolate per gram of seed at 8.5% moisture and 40% oil content or per gram of oil free meal at 8.5% moisture as indicated.
  • PROG Progoitrin 2-Hydroxy-3-butenyl glucosinolate ALLYL (Sinigrin) Allyl glucosinolate NAPOL (Napoleiferin) 2-Hydroxy-4-pentenyl glucosinolate
  • GLUCO Gluconapin 3-Butenyl glucosinolate 4-OH (4-OH Glucobrassicin) 4-Hydroxy-3-indolylmethyl glucosinolate
  • GLUCOBN Glucobrassicanapin
  • GLUCOBB Glucobrassicin 3-Indolylmethyl glucosinolate

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Physiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Steroid Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

L'invention se rapporte à des graines de Brassica ayant une teneur maximum en glucosinolates de 3,4 m moles par gramme de graines, de lignées végétales ou de la souche de cette plante qui donne ces graines, et à la farine dérivée de ces graines.
PCT/US1994/001869 1993-02-25 1994-02-22 Graines de brassica ayant des niveaux reduits de glucosinolates WO1994019929A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AT94910149T ATE192016T1 (de) 1993-02-25 1994-02-22 Brassica mit niedrigem glucosinolate gehalt
AU62703/94A AU696492B2 (en) 1993-02-25 1994-02-22 Low level glucosinolate (brassica)
DK94910149T DK0685993T3 (da) 1993-02-25 1994-02-22 Brassica med lavt glucosinolatindhold
US08/507,394 US5866762A (en) 1993-02-25 1994-02-22 Low level glucosinolate brassica
CA002157019A CA2157019C (fr) 1993-02-25 1994-02-22 Brassica a faible teneur en glucosinolate
DE69424155T DE69424155T2 (de) 1993-02-25 1994-02-22 Brassica mit niedrigem glucosinolate gehalt
EP94910149A EP0685993B1 (fr) 1993-02-25 1994-02-22 GRAINES DE $i(BRASSICA) AYANT DES NIVEAUX REDUITS DE GLUCOSINOLATES

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2323893A 1993-02-25 1993-02-25
US08/023,238 1993-02-25

Related Parent Applications (1)

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US2323893A Continuation 1993-02-25 1993-02-25

Related Child Applications (1)

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US09/240,831 Continuation US6225533B1 (en) 1993-02-25 1999-02-01 Low level glucosinolate Brassica

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WO1994019929A2 true WO1994019929A2 (fr) 1994-09-15
WO1994019929A3 WO1994019929A3 (fr) 1995-01-12

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US (2) US5866762A (fr)
EP (1) EP0685993B1 (fr)
AT (1) ATE192016T1 (fr)
AU (1) AU696492B2 (fr)
DE (1) DE69424155T2 (fr)
DK (1) DK0685993T3 (fr)
WO (1) WO1994019929A2 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0771878A1 (fr) * 1995-10-31 1997-05-07 Plant Genetic Systems N.V. Plantes à teneur réduite en glucosinolates
US6225533B1 (en) 1993-02-25 2001-05-01 Cargill, Incorporated Low level glucosinolate Brassica
WO2001037642A1 (fr) * 1999-11-26 2001-05-31 National Dairy Development Board Nouvelle lignee vegetale de brassica napus
KR100473484B1 (ko) * 1995-09-15 2005-07-18 존스 홉킨스 스쿨 오브 메디슨 겨자과식물의싹또는종자로부터추출된글루코시놀레이트와이소티오시아네이트및당해추출방법,이를포함하는암화학보호성식품,환제또는정제,및당해암화학보호성식품의제조방법

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100257369B1 (ko) * 1997-05-19 2000-05-15 구본준 횡전계방식액정표시장치
US6706953B2 (en) 1999-11-24 2004-03-16 Dhara Vegetable Oil And Food Company Limited Brassica napus plant line NUDB-38
ES2323644T3 (es) 2003-08-21 2009-07-22 Monsanto Technology Llc Desaturasas de acidos grasos procedentes de primula.
WO2005102310A1 (fr) 2004-04-16 2005-11-03 Monsanto Technology Llc Expression de desaturases d'acide gras de mais
WO2006052662A2 (fr) * 2004-11-04 2006-05-18 Monsanto Technology Llc Compositions d'huile a concentration elevee en acides gras polyinsatures
ITBO20050416A1 (it) * 2005-06-22 2006-12-23 Claudio Cerato Composizione per la cura e/o la prevenzione di attacchi da parte di agenti biologici
CA2645148C (fr) * 2006-03-10 2018-05-22 Monsanto Technology Llc Compositions d'huiles et de graines de soja et procedes de fabrication
CA2683735A1 (fr) * 2007-04-12 2008-10-23 Dow Agrosciences Llc Nouveaux cultivars de canola presentant des profils de productivite elevee et de stabilisation en acides gras
EP2379709B1 (fr) * 2008-12-18 2015-06-03 Dianaplantsciences, Inc. Culture de cellules végétales pour la production de produits naturels présentant une contamination aux glucosinolates réduite
US9480271B2 (en) 2009-09-15 2016-11-01 Monsanto Technology Llc Soybean seed and oil compositions and methods of making same
CA3041371A1 (fr) 2009-12-18 2011-06-23 Cargill Incorporated Plantes du genre brassica donnant des huiles a faible teneur totale en acides gras satures
US9695434B2 (en) 2010-05-25 2017-07-04 Cargill, Incorporated Brassica plants yielding oils with a low alpha linolenic acid content
WO2011150028A2 (fr) 2010-05-25 2011-12-01 Cargill, Incorporated Plantes du genre brassica donnant des huiles à faible teneur en acide alpha-linolénique
US8558065B2 (en) 2010-12-30 2013-10-15 Agrigenetics, Inc. Canola cultivar G31064
US9603322B2 (en) 2010-12-30 2017-03-28 Agrigenetics, Inc. Canola cultivars having high yield and stabilized fatty acid profiles

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3005039A1 (de) * 1980-02-11 1981-08-20 Siegfried 2301 Osdorf Bieder Verfahren und vorrichtung zur verbrennung von locker gelagerten feststoffen
US4889921A (en) * 1987-04-29 1989-12-26 The University Of Toronto Innovations Foundation Production of rapeseed protein materials
ATE110224T1 (de) * 1987-12-31 1994-09-15 Pioneer Hi Bred Int Produktion von raps mit erhöhtem ölsäuregehalt.
US5077071A (en) * 1989-09-06 1991-12-31 Epe Incorporated Oil extrusion process
CA2089265C (fr) * 1990-08-30 2002-02-26 Lorin R. Debonte Graines, plantes et huiles a profil d'acide gras modifie
EP0685993B1 (fr) 1993-02-25 2000-04-26 Cargill, Incorporated GRAINES DE $i(BRASSICA) AYANT DES NIVEAUX REDUITS DE GLUCOSINOLATES

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6225533B1 (en) 1993-02-25 2001-05-01 Cargill, Incorporated Low level glucosinolate Brassica
KR100473484B1 (ko) * 1995-09-15 2005-07-18 존스 홉킨스 스쿨 오브 메디슨 겨자과식물의싹또는종자로부터추출된글루코시놀레이트와이소티오시아네이트및당해추출방법,이를포함하는암화학보호성식품,환제또는정제,및당해암화학보호성식품의제조방법
EP0771878A1 (fr) * 1995-10-31 1997-05-07 Plant Genetic Systems N.V. Plantes à teneur réduite en glucosinolates
WO1997016559A1 (fr) * 1995-10-31 1997-05-09 Plant Genetic Systems, N.V. Plantes presentant une teneur reduite en glucosinolate
WO2001037642A1 (fr) * 1999-11-26 2001-05-31 National Dairy Development Board Nouvelle lignee vegetale de brassica napus

Also Published As

Publication number Publication date
DK0685993T3 (da) 2000-08-07
DE69424155D1 (de) 2000-05-31
DE69424155T2 (de) 2000-10-19
US5866762A (en) 1999-02-02
WO1994019929A3 (fr) 1995-01-12
US6225533B1 (en) 2001-05-01
EP0685993B1 (fr) 2000-04-26
AU696492B2 (en) 1998-09-10
EP0685993A1 (fr) 1995-12-13
AU6270394A (en) 1994-09-26
ATE192016T1 (de) 2000-05-15

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