+

WO1994011507A3 - Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna - Google Patents

Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna Download PDF

Info

Publication number
WO1994011507A3
WO1994011507A3 PCT/US1993/011295 US9311295W WO9411507A3 WO 1994011507 A3 WO1994011507 A3 WO 1994011507A3 US 9311295 W US9311295 W US 9311295W WO 9411507 A3 WO9411507 A3 WO 9411507A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna
hybridomas
production
recombinant antibodies
sequence replication
Prior art date
Application number
PCT/US1993/011295
Other languages
French (fr)
Other versions
WO1994011507A2 (en
Inventor
Thomas R Gingeras
Norman R M D Klinman
Cathy A Stillman
Phyllis-Jean Linton
Debra J Decker
Matthew C Biery
Original Assignee
Thomas R Gingeras
Norman R M D Klinman
Cathy A Stillman
Linton Phyllis Jean
Debra J Decker
Matthew C Biery
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thomas R Gingeras, Norman R M D Klinman, Cathy A Stillman, Linton Phyllis Jean, Debra J Decker, Matthew C Biery filed Critical Thomas R Gingeras
Priority to EP94902330A priority Critical patent/EP0628076A1/en
Priority to AU56737/94A priority patent/AU5673794A/en
Publication of WO1994011507A2 publication Critical patent/WO1994011507A2/en
Publication of WO1994011507A3 publication Critical patent/WO1994011507A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention is directed to a method for producing a recombinant protein having a preselected binding affinity and specificity for a given antigen. Generally, the method combines spleen fragment culture for the in vitro somatic mutation of B cells in response to antigen, followed by preferential amplification of RNA encoding heavy and light chains of desired antibodies produced by those B cells. The amplified RNA is then converted to DNA and incorporated into expression vectors; transfected host cells then express the antibody chains or a single chain antibody having preselected characteristics. Also disclosed are generic degenerate primer pools for the efficient amplification of RNA encoding mouse IgG.
PCT/US1993/011295 1992-11-19 1993-11-19 Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna WO1994011507A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP94902330A EP0628076A1 (en) 1992-11-19 1993-11-19 PRODUCTION OF MONOCLONAL RECOMBINANT ANTIBODIES WITHOUT THE USE OF HYBRIDOMAS BY $i(IN VITRO) SPLEEN FRAGMENT CULTURE COMBINED WITH ISOTHERMAL SELF-SUSTAINED SEQUENCE REPLICATION OF RNA
AU56737/94A AU5673794A (en) 1992-11-19 1993-11-19 Production of monoclonal recombinant antibodies without the use of hybridomas by (in vitro) spleen fragment culture combined with isothermal self-sustained sequence replication of rna

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97883592A 1992-11-19 1992-11-19
US07/978,835 1992-11-19

Publications (2)

Publication Number Publication Date
WO1994011507A2 WO1994011507A2 (en) 1994-05-26
WO1994011507A3 true WO1994011507A3 (en) 1994-07-07

Family

ID=25526436

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/011295 WO1994011507A2 (en) 1992-11-19 1993-11-19 Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna

Country Status (3)

Country Link
EP (1) EP0628076A1 (en)
AU (1) AU5673794A (en)
WO (1) WO1994011507A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6266899A (en) * 1998-10-05 2000-04-26 Ludwig Institute For Cancer Research Methods for producing human tumor antigen specific antibodies
EP1392866B1 (en) * 2001-05-14 2012-03-28 Henry Hongjun Ji Novel method for cloning variable domain sequences of immunological gene repertoire
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
EP3961214A1 (en) 2010-12-31 2022-03-02 BioAtla, Inc. Comprehensive monoclonal antibody generation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008800A1 (en) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Nucleic acid amplification by two-enzyme, self-sustained sequence replication

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008800A1 (en) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Nucleic acid amplification by two-enzyme, self-sustained sequence replication

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LINTON, P-J. ET AL.;: "Primary antibody-forming cells and secondary B cells are generated from separate precursor cell populations", CELL, vol. 59, 22 December 1989 (1989-12-22), CAMBRIDGE, NA US, pages 1049 - 1059 *

Also Published As

Publication number Publication date
AU5673794A (en) 1994-06-08
EP0628076A1 (en) 1994-12-14
WO1994011507A2 (en) 1994-05-26

Similar Documents

Publication Publication Date Title
AU664801B2 (en) Humanised antibodies
DE3883803D1 (en) Monoclonal antibodies to melanoma-associated antigens, hybrid cell lines that produce these antibodies, and their use.
IL161988A0 (en) Polycistronic expression of antibodies
CA2368734A1 (en) Method for preparing monoclonal antibody
ATE449853T1 (en) PRODUCTION AND USE OF HUMAN ANTIBODIES GENE BANKS (ßHUMAN ANTIBODIES LIBRARIESß)
WO2004061104A3 (en) Method for manufacturing recombinant polyclonal proteins
EP0332424A3 (en) Chimeric antibodies directed against human carcinoembryonic antigen
ATE377027T1 (en) TETRAVALENT ANTIBODIES CONSTRUCTS
ATE92959T1 (en) PRODUCTION OF CHIMAERIC ANTIBODIES.
WO1998046645A3 (en) Method for the production of antihuman antigen receptors and uses thereof
WO1998027114A3 (en) Mammalian cell surface antigens; related reagents
CA2508763A1 (en) Human antibody producing mouse and method for producing human antibody using the same
DE59007589D1 (en) Process for the production of hetero-bispecific antibodies.
DE3785186D1 (en) BINDING MOLECULE WITH SINGLE POLYPEPTIDE CHAIN.
AU4876690A (en) An expression system for production of chimeric monoclonal antibodies
IE810795L (en) Monoclonal antibodies against hepatitis b virus
NZ329314A (en) Hybridoma & method of preparing a monoclonal antibody reactive with hc gp-39, use & treatment of rheumatoid arthritis
WO1994011507A3 (en) Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna
DE69425843D1 (en) Recombinant vector for the exocellular use of single chain antibodies expressed in Bacillus Subtilis
KR960001122A (en) Recombinant single chain Fv antibody fragment and its use for immunopurification of hepatitis B virus surface antigen
ATE395422T1 (en) CHIMERIC ANTIBODIES FOR DELIVERY OF ANTIGENS TO SELECTED CELLS OF THE IMMUNE SYSTEM
EP0346702A3 (en) Transfectant cell lines which express isolated genes of the carcinoembryonic antigen family
EP0122814A3 (en) A method of cotransformation, cotransformed mouse cells producing at their surface human class ii antigens and the use of those cells to generate mouse hybridomas that produce monoclonal antibodies to those antigens
JPH05252986A (en) Heterobifunctional antibody and its utilization
Morrison New approaches to the production of monoclonal antibodies

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE

AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 1994902330

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2138009

Country of ref document: CA

WWP Wipo information: published in national office

Ref document number: 1994902330

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1994902330

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载