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WO1994003620A3 - Procede permettant d'accroitre l'expression de proteines virales - Google Patents

Procede permettant d'accroitre l'expression de proteines virales Download PDF

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Publication number
WO1994003620A3
WO1994003620A3 PCT/US1993/007299 US9307299W WO9403620A3 WO 1994003620 A3 WO1994003620 A3 WO 1994003620A3 US 9307299 W US9307299 W US 9307299W WO 9403620 A3 WO9403620 A3 WO 9403620A3
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WIPO (PCT)
Prior art keywords
viral proteins
increasing expression
proteins
methods
cell
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PCT/US1993/007299
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English (en)
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WO1994003620A2 (fr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed filed Critical
Priority to DK93918614T priority Critical patent/DK0652966T3/da
Priority to AT93918614T priority patent/ATE224956T1/de
Priority to MX9400188A priority patent/MX9400188A/es
Priority to JP6505519A priority patent/JP3048387B2/ja
Priority to EP93918614A priority patent/EP0652966B1/fr
Priority to DE69332334T priority patent/DE69332334T2/de
Priority to CA002140531A priority patent/CA2140531C/fr
Priority to AU48008/93A priority patent/AU680425B2/en
Publication of WO1994003620A2 publication Critical patent/WO1994003620A2/fr
Publication of WO1994003620A3 publication Critical patent/WO1994003620A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
    • C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Procédés d'expression et de sécrétion par recombinaison de protéines virales. Ces procédés consistent à utiliser des agents accompagnateurs compatibles afin d'entraîner les protéines en un mouvement de navette vers la surface cellulaire. De cette façon, la sortie de protéines produites par recombinaison de la cellule est facilitée, ce qui permet d'obtenir des rendements accrus et une purification plus aisée de la protéine requise.
PCT/US1993/007299 1992-07-29 1993-07-29 Procede permettant d'accroitre l'expression de proteines virales WO1994003620A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
DK93918614T DK0652966T3 (da) 1992-07-29 1993-07-29 Fremgangsmåde til forøgelse af ekspression af virale proteiner
AT93918614T ATE224956T1 (de) 1992-07-29 1993-07-29 Verfahren zur verstärkten expression von virusproteinen
MX9400188A MX9400188A (es) 1993-07-29 1993-07-29 Metodo para producir en forma recombinante una proteina y complejo quecomprende dicha proteina.
JP6505519A JP3048387B2 (ja) 1992-07-29 1993-07-29 ウイルスタンパク質の発現を増大する方法
EP93918614A EP0652966B1 (fr) 1992-07-29 1993-07-29 Procede permettant d'accroitre l'expression de proteines virales
DE69332334T DE69332334T2 (de) 1992-07-29 1993-07-29 Verfahren zur verstärkten expression von virusproteinen
CA002140531A CA2140531C (fr) 1992-07-29 1993-07-29 Methode pour augmenter l'expression de proteines virales
AU48008/93A AU680425B2 (en) 1992-07-29 1993-07-29 Method of increasing expression of viral proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/921,807 US5474914A (en) 1992-07-29 1992-07-29 Method of producing secreted CMV glycoprotein H
US07/921,807 1992-07-29

Publications (2)

Publication Number Publication Date
WO1994003620A2 WO1994003620A2 (fr) 1994-02-17
WO1994003620A3 true WO1994003620A3 (fr) 1994-05-11

Family

ID=25446002

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/007299 WO1994003620A2 (fr) 1992-07-29 1993-07-29 Procede permettant d'accroitre l'expression de proteines virales

Country Status (9)

Country Link
US (2) US5474914A (fr)
EP (2) EP1298215A3 (fr)
JP (1) JP3048387B2 (fr)
AT (1) ATE224956T1 (fr)
AU (1) AU680425B2 (fr)
CA (1) CA2140531C (fr)
DE (1) DE69332334T2 (fr)
DK (1) DK0652966T3 (fr)
WO (1) WO1994003620A2 (fr)

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US6855316B1 (en) * 1994-02-14 2005-02-15 University Of Hawaii Baculovirus produced Plasmodium falciparum vaccine
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US6156319A (en) * 1994-07-25 2000-12-05 The Trustees Of The University Of Pennsylvania Soluble herpesvirus glycoprotein complex vaccine
US5807557A (en) * 1994-07-25 1998-09-15 The Trustees Of The University Of Pennsylvania Soluble herpesvirus glycoprotein complex
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US6461814B1 (en) * 1997-01-15 2002-10-08 Dominic G. Spinella Method of identifying gene transcription patterns
WO2000039311A1 (fr) * 1998-12-31 2000-07-06 Advanced Research And Technology Institute Recepteur humain du facteur de croissance des fibroblastes utilise comme co-recepteur dans l'injection du virus 2 associe aux adenovirus
US6710173B1 (en) 1999-06-25 2004-03-23 Progenics Pharmaceuticals, Inc. Stabilized viral envelope proteins and uses thereof
US7108984B2 (en) * 2000-01-12 2006-09-19 Mount Sinai School Of Medicine Methods of identifying modulators of the FGF receptor
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US20030199092A1 (en) * 2002-01-11 2003-10-23 The Regents Of The University Of California Methods for increasing mRNA half-life in eukaryotic cells
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WO1992002628A1 (fr) * 1990-08-02 1992-02-20 Chiron Corporation Expression de glycoproteine-h de cytomegalovirus humain utilisant le systeme d'expression cellulaire de baculovirus-insectes
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EP0509841A2 (fr) * 1991-04-18 1992-10-21 Tonen Corporation Système de co-expression d'un gène de la protéine disolfure isomerase et d'un gène d'un polypeptide utile et procédé de production de ce polypeptide en utilisant un tel système
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WO1992002628A1 (fr) * 1990-08-02 1992-02-20 Chiron Corporation Expression de glycoproteine-h de cytomegalovirus humain utilisant le systeme d'expression cellulaire de baculovirus-insectes
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EP0509841A2 (fr) * 1991-04-18 1992-10-21 Tonen Corporation Système de co-expression d'un gène de la protéine disolfure isomerase et d'un gène d'un polypeptide utile et procédé de production de ce polypeptide en utilisant un tel système
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KAUFMAN, R.J. ET AL.: "Effect of von Willebrand factor coexpression on the synthesis and secretion of Factor VIII in Chinese Hamster Ovary cells", MOLECULAR AND CELLULAR BIOLOGY, vol. 9, no. 3, March 1989 (1989-03-01), pages 1233 - 1242 *
KAYE, J.F. ET AL.: "Glycoprotein H of human cytomegalovirus (HCMV) forms a stable complex with the HCMV UL115 gene product", JOURNAL OF GENERAL VIROLOGY, vol. 73, no. 10, October 1992 (1992-10-01), pages 2693 - 2698 *
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SPAETE, R.R. ET AL.: "CMV (Towne) glycoprotein H (gH) is complexed with GRP78 and GRP94", PROGRESS IN CYTOMEGALOVIRUS RESEARCH. M.P. LANDINI EDITOR, 1991, ELSEVIER SCIENCE PUBLISHER, pages 133 - 136 *
SPAETE, R.R. ET AL.: "Coexpression of truncated Human Cytomegalovirus gH with UL115 gene product or the truncated Human Fibroblast growth Factor Receptor results in transport of gH to the cell surface", VIROLOGY, vol. 193, no. 2, February 1993 (1993-02-01), pages 853 - 861 *

Also Published As

Publication number Publication date
DK0652966T3 (da) 2003-02-03
CA2140531A1 (fr) 1994-02-17
AU680425B2 (en) 1997-07-31
EP0652966A1 (fr) 1995-05-17
JP3048387B2 (ja) 2000-06-05
CA2140531C (fr) 2000-03-28
WO1994003620A2 (fr) 1994-02-17
ATE224956T1 (de) 2002-10-15
DE69332334D1 (de) 2002-10-31
DE69332334T2 (de) 2003-08-07
EP1298215A3 (fr) 2007-09-26
EP1298215A2 (fr) 2003-04-02
EP0652966B1 (fr) 2002-09-25
US5474914A (en) 1995-12-12
US5767250A (en) 1998-06-16
AU4800893A (en) 1994-03-03
JPH07509370A (ja) 1995-10-19

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