WO1993016715A1 - Facteur 1 de stimulation de fibroblastes et detection anticipee de la fibrose - Google Patents
Facteur 1 de stimulation de fibroblastes et detection anticipee de la fibrose Download PDFInfo
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- WO1993016715A1 WO1993016715A1 PCT/US1993/001554 US9301554W WO9316715A1 WO 1993016715 A1 WO1993016715 A1 WO 1993016715A1 US 9301554 W US9301554 W US 9301554W WO 9316715 A1 WO9316715 A1 WO 9316715A1
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- fsf
- polypeptide
- sample
- fibrotic scarring
- antibody
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- 230000035945 sensitivity Effects 0.000 description 1
- 239000004017 serum-free culture medium Substances 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- mice Animals and Schistosomiasis infection C57BL/6NcrLBr female mice (18 to 20 g; Taconic Farms, Inc., Germantown, NY) were infected by the intraperitoneal injection of 35-50 cercariae of S. mansoni (Puerto Guatemalan strain) suspended in 0.5 ml sterile saline. The mice were euthanized eight weeks later by inducing C0 2 narcosis and their livers were placed in cold Hanks'' buffered salt solution (HBSS) . Isolation of granulomas and preparation of crranuloma supernatant. Isolation of granulomas was performed as described previously (Wyler et al. , Science.
- Antibody-treated cells were sorted FACStar Plus flow cytometry and analyzed by a FACScan (Becton- Dickinson) or a Coulter Epics 541 (Coulter Electronics Inc. , Hialeah, FL) flow cytometer. The fields were gaited to exclude autofluorescent and dead cells.
- the CD4+ sorted cells were washed and suspended (0.6-1.0 x 10 6 /ml) in serum-free medium RPMI 1640 supplemented with 0.3 g/ml bovine serum albumin (BSA; Sigma, St. Louis, MO) and incubated for 24h at 30°C in 5% C0 2 - 95% air humid atmosphere.
- BSA bovine serum albumin
- the beads were pelleted (12,000 g x 5 min) , washed twice in phosphate-buffered 0.15 M NaCl and then boiled for 5 min in sample buffer (lOmM Tris-HCl; 2% sodium dodecyl sulfate; 5% glycerol; 2% dithiothreitol; 0.05% pyronin Y; pH 6.8). Supernatant from these treated beads were subjected to electrophoresis in a 70 x 70 x 0.5 ui slab gel of 10% acrylamide (BioRad Labs, Richmond, CA) using standard method (Laemmli, Nature 227:680 (1970)).
- proteins were transferred electrophoretically (l-2h; 70 volts) onto nitrocellulose paper (BioRad) using standard procedures.
- the nitrocellulose paper was then exposed to X-ray film (X- OMAT, AR; Kodak, Rochester, NY) for 72 h at -70°C.
- Anti-FSF-1 antibody detected FSF-1 in both purified form and in crude granuloma supernatant in a dot-blot ELISA assay (Fig. 5) .
- the antibody did not detect acidic FGF or PDGF, mitogens that were detected with the appropriate homologous antibodies (Fig. 5) .
- CD4 + cells are stimulated "" in vivo to produce FsF-1 in response to egg antigens. Based on our current observation, such production may continue at least briefly when the lymphocytes are dissociated from the eggs and antigen-presenting cells.
- FsF-1 not only is secreted but also can remain associated with the surface of CD4 + lymphocytes suggests that in addition to the action of the secreted cytokine, direct contact between membrane-associated FsF-1 positive lymphocytes and fibroblasts might stimulate fibroblast growth.
- fibroblast growth factors some of which were purified from other sources and were recognized for this biological property (PDGF [Ross et al.. Cell 46:155 (1986)], FGF [Gospodarowicz et al. , J. Biol. Chem. 250:2515 (1975); Gospodarowicz et al. , J. Biol. 253.:3736 (1978)]), and heparin-binding epidermal growth factor [HB-EGF;Higashiyama et al.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
Procédé d'identification d'individus susceptibles de présenter une cicatrisation due à la fibrose. Le procédé consiste à prélever un échantillon chez un individu atteint d'une maladie inflammatoire chronique, à mettre l'échantillon en contact avec un anticorps spécifique au facteur 1 de stimulation de fibroblastes (FSF-1) dans des conditions permettant la formation d'un complexe immun, et à détecter l'éventuelle augmentation du taux relatif du complexe immun, laquelle indiquerait une vulnérabilité à la cicatrisation due à la fibrose. On a également prévu des polypeptides de FSF-1 et des anticorps spécifiques au FSF-1.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US840,426 | 1977-10-07 | ||
| US84042692A | 1992-02-24 | 1992-02-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1993016715A1 true WO1993016715A1 (fr) | 1993-09-02 |
Family
ID=25282348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1993/001554 WO1993016715A1 (fr) | 1992-02-24 | 1993-02-19 | Facteur 1 de stimulation de fibroblastes et detection anticipee de la fibrose |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO1993016715A1 (fr) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996026953A1 (fr) * | 1995-02-28 | 1996-09-06 | New England Medical Center Hospitals, Inc. | FsF-1 ET LA DETECTION PRECOCE DES FIBROSES |
| US6017719A (en) * | 1994-06-14 | 2000-01-25 | Nexell Therapeutics, Inc. | Positive and positive/negative cell selection mediated by peptide release |
| US6214968B1 (en) | 1992-02-24 | 2001-04-10 | New England Medical Center Hospitals, Inc. | Fibroblast stimulating growth factor 1 (FsF-1) and the early detection of fibrosis |
-
1993
- 1993-02-19 WO PCT/US1993/001554 patent/WO1993016715A1/fr active Application Filing
Non-Patent Citations (2)
| Title |
|---|
| D. CATTY, "Antibodies, Volume 1, A Practical Approach", Published 1989, by IRL PRESS, see pages 19-79. * |
| JOURNAL OF IMMUNOLOGY, Volume 132, Number 6, issued June 1984, D.J. WYLER et al., "Fibroblast Stimulation in Schistosomiasis", pages 3142-3148. * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6214968B1 (en) | 1992-02-24 | 2001-04-10 | New England Medical Center Hospitals, Inc. | Fibroblast stimulating growth factor 1 (FsF-1) and the early detection of fibrosis |
| US6017719A (en) * | 1994-06-14 | 2000-01-25 | Nexell Therapeutics, Inc. | Positive and positive/negative cell selection mediated by peptide release |
| WO1996026953A1 (fr) * | 1995-02-28 | 1996-09-06 | New England Medical Center Hospitals, Inc. | FsF-1 ET LA DETECTION PRECOCE DES FIBROSES |
| WO1996026954A1 (fr) * | 1995-02-28 | 1996-09-06 | New England Medical Center Hospitals, Inc. | Fsf-1 et la detection precoce des fibroses |
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