WO1993013196A3 - Method for increasing recombinant protein production - Google Patents
Method for increasing recombinant protein production Download PDFInfo
- Publication number
- WO1993013196A3 WO1993013196A3 PCT/US1992/011188 US9211188W WO9313196A3 WO 1993013196 A3 WO1993013196 A3 WO 1993013196A3 US 9211188 W US9211188 W US 9211188W WO 9313196 A3 WO9313196 A3 WO 9313196A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- recombinant protein
- protein production
- increasing
- increasing recombinant
- cultured
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 2
- 230000014616 translation Effects 0.000 title 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 abstract 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 abstract 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 abstract 1
- 229930195712 glutamate Natural products 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01045—Glucosylceramidase (3.2.1.45), i.e. beta-glucocerebrosidase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/495—Transforming growth factor [TGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Methods and compositions for increasing the production of recombinant proteins are provided. In one aspect, eukaryotic expression systems are cultured in media containing an appropriate amount of transforming growth factor-β. In another aspect, eukaryotic expression systems are cultured in glutamate-containing, essentially glutamine-free media.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81332491A | 1991-12-24 | 1991-12-24 | |
| US07/813,324 | 1991-12-24 | ||
| US87668092A | 1992-04-30 | 1992-04-30 | |
| US07/876,680 | 1992-04-30 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1993013196A2 WO1993013196A2 (en) | 1993-07-08 |
| WO1993013196A3 true WO1993013196A3 (en) | 1993-10-28 |
Family
ID=27123724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1992/011188 WO1993013196A2 (en) | 1991-12-24 | 1992-12-23 | Method for increasing recombinant protein production |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO1993013196A2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11124760B2 (en) | 2014-03-24 | 2021-09-21 | Biogen Ma Inc. | Methods for overcoming glutamine deprivation during mammalian cell culture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0307247A2 (en) * | 1987-09-11 | 1989-03-15 | Genentech, Inc. | A method for culturing recombinant cells |
| WO1990003430A1 (en) * | 1988-09-23 | 1990-04-05 | Cetus Corporation | Cell culture medium for enhanced cell growth, culture longevity and product expression |
| WO1990007573A1 (en) * | 1988-12-23 | 1990-07-12 | Genzyme Corporation | Enzymatically active recombinant glucocerebrosidase |
-
1992
- 1992-12-23 WO PCT/US1992/011188 patent/WO1993013196A2/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0307247A2 (en) * | 1987-09-11 | 1989-03-15 | Genentech, Inc. | A method for culturing recombinant cells |
| WO1990003430A1 (en) * | 1988-09-23 | 1990-04-05 | Cetus Corporation | Cell culture medium for enhanced cell growth, culture longevity and product expression |
| WO1990007573A1 (en) * | 1988-12-23 | 1990-07-12 | Genzyme Corporation | Enzymatically active recombinant glucocerebrosidase |
Non-Patent Citations (4)
| Title |
|---|
| ABSTRACTS OF PAPERS OF THE 196th NATIONAL MEETING OF THE AMERICAN CHEMICAL SOCIETY; LOS ANGELES, CALIFORNIA; September 25-30, 1988; Abstract MBTD 141; FIELD, R.P.: 'Suspension growth of recombinant CHO cells in serum-free and glutamine-free medium' * |
| BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS vol. 138, no. 2, 31 July 1986, DULUTH, MINNESOTA US pages 974 - 980 VARGA, J. & JIMENEZ, S.A. 'Stimulation of normal human fibroblast collagen production and processing by transforming growth factor-beta' * |
| CLINICAL RESEARCH vol. 35, no. 3, April 1987, page 725A WIKNER, N.E. ET AL. 'Transforming growth factor-beta stimulates the expression of fibronectin by human keratinocytes' * |
| JOURNAL OF DERMATOLOGY vol. 18, no. 5, May 1991, TOKYO, JP pages 252 - 257 HASHIRO, M. ET AL. 'Stimulation of fibronectin secretion in cultured human keratinocytes by transforming growth factor-beta not by other growth inhibitory substances' * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1993013196A2 (en) | 1993-07-08 |
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