WO1993008683A1 - Herbicide resistant plants - Google Patents
Herbicide resistant plants Download PDFInfo
- Publication number
- WO1993008683A1 WO1993008683A1 PCT/GB1992/001989 GB9201989W WO9308683A1 WO 1993008683 A1 WO1993008683 A1 WO 1993008683A1 GB 9201989 W GB9201989 W GB 9201989W WO 9308683 A1 WO9308683 A1 WO 9308683A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- herbicide
- plant
- clomazone
- maize
- plants
- Prior art date
Links
- 239000004009 herbicide Substances 0.000 title claims abstract description 40
- 230000002363 herbicidal effect Effects 0.000 title claims abstract description 37
- 241000196324 Embryophyta Species 0.000 claims abstract description 52
- KIEDNEWSYUYDSN-UHFFFAOYSA-N clomazone Chemical compound O=C1C(C)(C)CON1CC1=CC=CC=C1Cl KIEDNEWSYUYDSN-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000005499 Clomazone Substances 0.000 claims abstract description 41
- 231100000636 lethal dose Toxicity 0.000 claims abstract description 7
- 240000008042 Zea mays Species 0.000 claims description 31
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 20
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 18
- 235000009973 maize Nutrition 0.000 claims description 18
- 231100000350 mutagenesis Toxicity 0.000 claims description 12
- 238000002703 mutagenesis Methods 0.000 claims description 12
- QXDOFVVNXBGLKK-UHFFFAOYSA-N 3-Isoxazolidinone Chemical class OC1=NOCC1 QXDOFVVNXBGLKK-UHFFFAOYSA-N 0.000 claims description 8
- 102100031699 Choline transporter-like protein 1 Human genes 0.000 claims 1
- 101000940912 Homo sapiens Choline transporter-like protein 1 Proteins 0.000 claims 1
- 230000009471 action Effects 0.000 abstract description 9
- 239000002962 chemical mutagen Substances 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 16
- 238000005204 segregation Methods 0.000 description 11
- 230000035772 mutation Effects 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 7
- PLUBXMRUUVWRLT-UHFFFAOYSA-N Ethyl methanesulfonate Chemical compound CCOS(C)(=O)=O PLUBXMRUUVWRLT-UHFFFAOYSA-N 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 239000000049 pigment Substances 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 4
- 229930002875 chlorophyll Natural products 0.000 description 4
- 235000019804 chlorophyll Nutrition 0.000 description 4
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 4
- 210000005069 ears Anatomy 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000004576 sand Substances 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 101100060388 Arabidopsis thaliana CLT1 gene Proteins 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000219195 Arabidopsis thaliana Species 0.000 description 2
- 108700003861 Dominant Genes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 240000001140 Mimosa pudica Species 0.000 description 2
- 235000016462 Mimosa pudica Nutrition 0.000 description 2
- 239000005662 Paraffin oil Substances 0.000 description 2
- 244000090599 Plantago psyllium Species 0.000 description 2
- 241001464837 Viridiplantae Species 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 244000038559 crop plants Species 0.000 description 2
- 230000002939 deleterious effect Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000010187 selection method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 150000003505 terpenes Chemical class 0.000 description 2
- OINNEUNVOZHBOX-QIRCYJPOSA-K 2-trans,6-trans,10-trans-geranylgeranyl diphosphate(3-) Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CC\C(C)=C\COP([O-])(=O)OP([O-])([O-])=O OINNEUNVOZHBOX-QIRCYJPOSA-K 0.000 description 1
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- OINNEUNVOZHBOX-XBQSVVNOSA-N Geranylgeranyl diphosphate Natural products [P@](=O)(OP(=O)(O)O)(OC/C=C(\CC/C=C(\CC/C=C(\CC/C=C(\C)/C)/C)/C)/C)O OINNEUNVOZHBOX-XBQSVVNOSA-N 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 241001180649 Myrcia group Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241001148683 Zostera marina Species 0.000 description 1
- 230000009102 absorption Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- ANWUQYTXRXCEMZ-NYABAGMLSA-L chlorophyllide a Chemical compound C1([C@H](C2=O)C(=O)OC)=C(N3[Mg]N45)C2=C(C)\C3=C\C(=N2)C(CC)=C(C)\C2=C\C4=C(C=C)C(C)=C5\C=C/2[C@@H](C)[C@H](CCC(O)=O)C1=N\2 ANWUQYTXRXCEMZ-NYABAGMLSA-L 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 230000007614 genetic variation Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011086 glassine Substances 0.000 description 1
- 208000006278 hypochromic anemia Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- NUHSROFQTUXZQQ-UHFFFAOYSA-N isopentenyl diphosphate Chemical compound CC(=C)CCO[P@](O)(=O)OP(O)(O)=O NUHSROFQTUXZQQ-UHFFFAOYSA-N 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000001844 prenyl group Chemical group [H]C([*])([H])C([H])=C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical compound OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4684—Zea mays [maize]
Definitions
- This invention relates to herbicide resistant maize plants.
- the purpose in providing crop plants which resist the action of a herbicide is to facilitate the destruction of weeds growing between the plants by the overall application of a herbicidally effective concentration of a herbicide which would destroy the crop plant in its normal, that is herbicide sensitive, state.
- Such resistant plants are also useful for use in a locus of any short term carry-over of herbicide from a previous crop.
- tissue culture techniques where spontaneous somaclonal variation occurs in the presence or absence of a utagen. By applying to the cultures some form of selection pressure it is possible to recover cells which resist that pressure. Depending on the plant species it is sometimes possible to regenerate whole plants from the resistant cells. Such tissue culture selection methods have been used in the past to select for resistance to herbicides.
- Pollen mutagenesis is discussed at pages 150 and 151. Pollen mutagenesis gives a relatively high frequency of variation in the M- generation compared with other available procedures. However, no suggestion is made in respect of the use of pollen mutagenesis for generation of mutants which display resistance to herbicide action. Section III (page 149) describes some of the difficulties of the use of mutagenesis as a source of genetic variation. One important aspect is the method by which the population of putative mutants is screened for useful phenotypes. Mutagenesis, of pollen or seed, has previously been used to generate large populations of random mutations in plants.
- Clomazone [2-(2-chlorophenyl)methyl-4,4- dimethyl-3-isoxazolidinone] , is a selective herbicide for use on soybean.
- a single pre-plant application of clomazone controls many grasses and some broad-leafed weeds. This herbicide reduces or stops the accumulation of plastid pigments in susceptible species by inhibiting an enzyme of the terpenoid pathway, resulting in white, yellow or pale green plants.
- the precise enzymatic site of action of clomazone is not known but some of the suggested sites of action are: (a) isopentenyl pyrophosphate enzyme, (b) prenyl transferase ⁇ , (c) enzymatic phytylation of chlorophyllide, and, (d) some enzyme downstream of geranyl-geranyl- pyrophosphate production in the terpenoid pathway. It is believed that differences in selectivity between tolerant species such as soybean and sensitive species such as maize is not due to to differential absorption, translocation or metabolism but is accounted for by some differences between the species which occur at the site of action.
- Maize is very sensitive to the action of clomazone and the introduction of tolerance to this herbicide would be beneficial to farmers in allowing greater flexibility in grass and broad—leafed weed control.
- An object of the present invention is to provide a herbicide resistant maize. According to the present invention there is provided a plant having an increased level of tolerance to a normally lethal dose of a herbicide of the isoxazolidinone class.
- the herbicide is clomazone [2-(2- chlorobenzyl)-4,4-dimethyl-l,2- oxazolidin-2-one]. It is further provided by the invention a maize plant having an increased level of tolerance to a normally lethal dose of a herbicide of the isoxazolidinone class.
- the maize plant is derived by pollen mutagenesis from the maize inbred line designated UE95.
- the invention further provides a hybrid maize which is tolerant of normally lethal dose of an isoxazolidinone herbicide, of which at least one of the parents is an isoxazolidinone-tolerant maize plant as hereinabove defined.
- the invention also provides seed and progeny of the aforesaid plants.
- the invention further provides seed of the tolerant mutant designated UE95/CLT1, a sample of which has been deposited with the American Type Culture Collection under the terms of the Budapest Treaty, prior to the filing of this application.
- the resistance trait segregates in the deposited seeds but the tolerant mutants may be obtained by growing the seeds under the conditions specified for screening in Example 2 below.
- the tolerance exhibits a 3:1 segregation and at the higher concenration at a ratio of 1:2:1 of homozygous tolerant: heterozygous tolerant: homozygous sensitive.
- Clomazone a member of the isoxazolidinone family of herbicides,is the active ingredient of a commercial herbicide known as Command (Trade Mark, FMC Corporation): its chemical name is 2-(2-chloro- benzyl)-4,4-dimethyl-l,2-oxazolidin-3-one
- the maize plants of this invention are resistant to clomazone but, as is not uncommon in such cases, it is expected that some degree of cross-resistance will exist in respect of other isoxazolidinone herbicides and other herbicidally active compounds which have similar modes of action.
- Maize is very sensitive to clomazone. However, although we have demonstrated that genotypic variation for tolerance of clomazone does exist in maize, the level of that tolerance, even in the most tolerant genotype, is well below the field application rate required for efficient herbicidal action by clomazone. Table I classifies 40 commercial maize inbreds according to their degrees of tolerance. TABLE 1 INBRED CORN LINES CLASSIFIED ACCORDING TO THEIR RESPONSE TO CLOMAZONE AT A CONCENTRATION OF 0.2 PPM (97.23 G/HA)
- the preferred method of creating the clomazone-tolerant plants of this invention is selection from a population of random mutants created via pollen-mutagenesis in the manner described in our International Patent Application Number PCT/GB90/00753.
- somaclonal variation may occur it is required that a culture of plant tissue be established. This requirement restricts the choice of genotype which may be used as it is not always possible to regenerate whole plants from the cultured tissue.
- mutagenised pollen may be applied to any recipient maize genotype, including commercially important and well-established elite breeding lines. Also the rate of occurrence of undesirable mutations which somaclonal variation is known to produce is unexpectedly reduced.
- selection is carried out at at the M. generation, with the result that only dominant mutations are selected. Also, being carried out on whole plants or on the seed pre-emergence, or both, allows the herbicide concentration to mimic the field conditions more closely than is possible with the application of the selection pressure of the herbicide to a tissue culture.
- tissue culture selection method whole plants have to be regenerated from the tissue and grown to maturity before any indication of the performance of the progeny under field application rates of the herbicide can be obtained.
- selection is made directly on the plants under concentrations of herbicide which are comparable to those which are recommended for normal weed-killing activity in the field.
- Figure 1 is a flow-chart showing the derivation of several generations of progeny from plants generated by this invention
- Figure 2 shows the chemical structure of clomazone
- Figure 3 shows the relative chlorophyll content (measured with a SPAD 502) of (a) control UE95 seedling (b) a wild-type UE95 seedling growing in 1.72 ppm clomazone and (c) the resistant UE95 mutant CLT1 growing in 1.75 ppm clomazone; and.
- Figure 4 shows the chlorophyll fluorescence transients of (A) control UE95 seedling (B) a wild-type UE95 seedling growing in 1.72 ppm clomazone and (C) the resistant UE95 mutant CLTl growing in 1.75 ppm clomazone
- FIG. 5 shows the visual assessment score of clomazone injury in Arabidopsis thaliana.
- the invention will now be described, by way of illustration, in the following Examples.
- EMS ethyl methane sulphonate
- Fresh pollen grains with anthers were harvested from a total of twenty tassels of field grown maize inbred line UE95. Pollen grains were separated from the anthers using a Glassine (Trade Mark) bag.
- CLT1 The resistant plant, designated CLT1
- CLT was transplanted and grown to maturity.
- CLT was self-pollinated and backcrossed to unmodified UE95.
- CLTl was crossed to the line BD68 to provide F. seed.
- This F. seed was also grown to maturity and self-pollinated to obtain F- seed for an RFLP linkage analysis, and backcrossed to BD68 again to provide BC ⁇ seed for the conversion of BD68.
- EXAMPLE 4 - GENETIC ANALYSIS BC ⁇ seed (150) of CLTl were planted and sprayed with clomazone in the manner described above. After 9 days growth the plants were evaluated for tolerance. Out of the 150 seeds only 76 seed germinated and grew.
- BC.S seed from 19 ears derived from CLTl was rescreened in the above manner. However, 100% silica sand was used to increase the effective dose of herbicide to the plants. After nine days growth the plants were evaluated for tolerance. On this occasion, it was possible to distinguish homozygous tolerant seedlings from heterozygous tolerant seedlings. Seedlings derived from each ear appeared to segregate in a 1:2:1 manner for tolerance to clomazone (Table 2). If homozygous tolerant and heterozygous tolerant seedlings were classed together, the seedlings exhibited a 3:1 segregation (Table 3). Both of these segregation ratios are indicative of a single gene for clomazone tolerance.
- Resistant plants were used in reciprocal back-crosses with homozygous, herbicide sensitive UE95.
- the frequency of resistant progeny in the M..BC, or M ⁇ BC 2 generations was found by treatment with clomazone and counting the survivors.
- the ratio of resistant to sensitive plants in the progeny of the backcross was not significantly different from 1:1 for all of the mutants, indicating that resistance is controlled by a single dominant gene.
- the M.BC plants were self pollinated to give generation M 1 BC.S. which was again self-pollinated to give M.BC.S,. From that generation it was possible to identify, by the fact that the resistant trait is non-segregating, lines which are homozygous for the trait. These homozygous lines selected for use in the production of F, hybrids which possess resistance to clomazone or for further breeding work.
- Arabidopsis thaliana (ecotypes Landsberg erecta and Columbia) M 2 seed derived from
- EMS-treated M. seed were purchased from Lehle Seeds (Tucson, AZ85718, USA). Seeds were surfaced-sterilised by sequential washing in 95% ethanol (7 minutes), 10% Domestos (Trade Mark) (10 minutes)and autoclaved water (3 washes). Seeds were spread in clear plastic dishes on sterile nutrient medium (half-strength MS salts and vitamins, 10 g/1 sucrose, 8 g/1 Phytagar, pH 5.8) supplemented with herbicide, and sealed with laboratory film. Seeds were allowed to germinate
- Arabidopsis (Landsberg erecta) was monitored over a concentration range of from zero to lOO M.
- the first leaf proved more sensitive to clomazone than the cotyledon.
- the first leaf was fully bleached by 5//M clomazone, whereas the cotyledon was incompletely injured at this concentration, ( Figure 5)
- Routine screening for clomazone tolerant mutants was performed on 5//M clomazone, by visually assessing the first leaf for tolerance to bleaching. A total of 175,000 M 2 seed were evaluated by the standard screening procedure. From one parental M. group, four tolerant candidate seedlings were identified (Landsberg erecta).
- the M 3 progeny derived from one of the selected M 2 seedlings were evaluated for inheritance of the clomazone tolerance trait. 3 seed were germinated on 5//M clomazone under standard culture conditions. All 89 germinated , seedlings exhibited greater tolerance to the bleaching action of clomazone, in comparison with the wild-type seedlings (Landsberg erecta). There was no evidence for segregation of the tolerance trait among the M, population. This demonstrated that the selected M_ parent of these progeny exhibited heritable tolerance to clomazone, and was genetically homozygous for the tolerance trait.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP92922546A EP0610338A1 (en) | 1991-10-31 | 1992-10-30 | Herbicide resistant plants |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9123159.7 | 1991-10-31 | ||
GB919123159A GB9123159D0 (en) | 1991-10-31 | 1991-10-31 | Herbicide resistant plants |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1993008683A1 true WO1993008683A1 (en) | 1993-05-13 |
Family
ID=10703877
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB1992/001989 WO1993008683A1 (en) | 1991-10-31 | 1992-10-30 | Herbicide resistant plants |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0610338A1 (en) |
AU (1) | AU2806292A (en) |
GB (1) | GB9123159D0 (en) |
WO (1) | WO1993008683A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK0508161T3 (en) * | 1991-04-08 | 2000-08-14 | American Cyanamid Co | Use of wheat resistant to AHAS-inhibiting herbicide as well as its selection method |
DE19838360A1 (en) * | 1998-04-22 | 1999-10-28 | Biotronik Mess & Therapieg | Vascular electrode line |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4443971A (en) * | 1979-10-16 | 1984-04-24 | Cornell Research Foundation, Inc. | Herbicide-tolerant plants |
WO1985003085A1 (en) * | 1984-01-16 | 1985-07-18 | Mta Szegedi Biológiai Központja | Process for the production of mixotrophic tissue cultures and herbicide-resistant plants |
EP0284419A1 (en) * | 1987-03-27 | 1988-09-28 | Pioneer Hi-Bred International, Inc. | Anther microspore-based selection process |
WO1990014000A1 (en) * | 1989-05-17 | 1990-11-29 | Imperial Chemical Industries Plc | Herbicide resistant maize |
EP0436484A2 (en) * | 1989-12-29 | 1991-07-10 | Monsanto Company | Safening mixtures of sulfonylurea and acetanilide herbicides |
-
1991
- 1991-10-31 GB GB919123159A patent/GB9123159D0/en active Pending
-
1992
- 1992-10-30 EP EP92922546A patent/EP0610338A1/en not_active Withdrawn
- 1992-10-30 AU AU28062/92A patent/AU2806292A/en not_active Abandoned
- 1992-10-30 WO PCT/GB1992/001989 patent/WO1993008683A1/en not_active Application Discontinuation
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4443971A (en) * | 1979-10-16 | 1984-04-24 | Cornell Research Foundation, Inc. | Herbicide-tolerant plants |
WO1985003085A1 (en) * | 1984-01-16 | 1985-07-18 | Mta Szegedi Biológiai Központja | Process for the production of mixotrophic tissue cultures and herbicide-resistant plants |
EP0284419A1 (en) * | 1987-03-27 | 1988-09-28 | Pioneer Hi-Bred International, Inc. | Anther microspore-based selection process |
WO1990014000A1 (en) * | 1989-05-17 | 1990-11-29 | Imperial Chemical Industries Plc | Herbicide resistant maize |
EP0436484A2 (en) * | 1989-12-29 | 1991-07-10 | Monsanto Company | Safening mixtures of sulfonylurea and acetanilide herbicides |
Non-Patent Citations (1)
Title |
---|
Week 9042, Derwent Publications Ltd., London, GB; AN 90-314832 * |
Also Published As
Publication number | Publication date |
---|---|
AU2806292A (en) | 1993-06-07 |
EP0610338A1 (en) | 1994-08-17 |
GB9123159D0 (en) | 1991-12-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4517763A (en) | Hybridization process utilizing a combination of cytoplasmic male sterility and herbicide tolerance | |
US4658085A (en) | Hybridization using cytoplasmic male sterility, cytoplasmic herbicide tolerance, and herbicide tolerance from nuclear genes | |
Kølster et al. | Near‐Isogenic Barley Lines with Genes for Resistance to Powdery Mildew 1 | |
US5162602A (en) | Corn plants tolerant to sethoxydim and haloxyfop herbicides | |
Adipala et al. | Reaction of maize cultivars from Uganda to Exserohilum turcicum. | |
EP0472573B1 (en) | Herbicide resistant maize | |
Bond | Recent developments in breeding field beans (Vicia faba L.). | |
Albertsen et al. | Genetics and comparative growth morphology of fasciation in soybeans (Glycine max [L.] Merr.) | |
US5428001A (en) | Method for combating weeds among maize plants | |
Hallauer | Breeding systems | |
AU663856B2 (en) | Herbicide resistant plants | |
Rai et al. | Pearl millet improvement at ICRISAT-an update | |
US5606823A (en) | Cover crop system | |
Banga | Heterosis and its utilization | |
RU2128426C1 (en) | Method of preparing mutant corn, zea mays | |
Hu et al. | Adult plant phenotype of the Rp1-DJ compound rust resistance gene in maize | |
Lucken | The breeding and production of hybrid wheat | |
WO1993008683A1 (en) | Herbicide resistant plants | |
US6121525A (en) | Inbred corn line ZS4199 | |
US6069304A (en) | Inbred sunflower line PHA232 | |
US5124504A (en) | Nitrate-tolerant soybean | |
AU692165B2 (en) | Cover crop system | |
Chicaiza | Genetic control of leaf rust in barley | |
Tumwesigye | Heterosis and combining ability in common Bean (Phaseolus Vulgaris L) in Kenya | |
Kyanam | Advancements in Sorghum Breeding to Address Emerging Challenges |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AU BB BG BR CA CS FI HU JP KP KR LK MG MN MW NO PL RO RU SD US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL SE BF BJ CF CG CI CM GA GN ML MR SN TD TG |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
WWE | Wipo information: entry into national phase |
Ref document number: 1992922546 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref country code: US Ref document number: 1994 232162 Date of ref document: 19940811 Kind code of ref document: A Format of ref document f/p: F |
|
WWP | Wipo information: published in national office |
Ref document number: 1992922546 Country of ref document: EP |
|
NENP | Non-entry into the national phase |
Ref country code: CA |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1992922546 Country of ref document: EP |