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WO1992018599A1 - Additif a action detergente renfermant une cellulase et une protease - Google Patents

Additif a action detergente renfermant une cellulase et une protease Download PDF

Info

Publication number
WO1992018599A1
WO1992018599A1 PCT/DK1992/000116 DK9200116W WO9218599A1 WO 1992018599 A1 WO1992018599 A1 WO 1992018599A1 DK 9200116 W DK9200116 W DK 9200116W WO 9218599 A1 WO9218599 A1 WO 9218599A1
Authority
WO
WIPO (PCT)
Prior art keywords
protease
cellulase
detergent
detergent composition
detergent additive
Prior art date
Application number
PCT/DK1992/000116
Other languages
English (en)
Inventor
Peter Wagner
Rie Tsuchiya
Original Assignee
Novo Nordisk A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8096968&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1992018599(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk A/S filed Critical Novo Nordisk A/S
Priority to BR9205921A priority Critical patent/BR9205921A/pt
Priority to EP92909626A priority patent/EP0581839B1/fr
Priority to JP4508964A priority patent/JPH06506712A/ja
Priority to DE69212150T priority patent/DE69212150T2/de
Publication of WO1992018599A1 publication Critical patent/WO1992018599A1/fr
Priority to FI934656A priority patent/FI934656A0/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase

Definitions

  • the present invention relates to a detergent additive and detergent composition
  • a detergent additive and detergent composition comprising a cellulase and a protease i the presence of which the cellulase exhibits improved stabilit properties.
  • cellulases are sensitiv to the action of proteases, i.e. that in the presence o proteases commonly employed in detergents, cellulases ar degraded to lower molecular weight polypeptides resulting i inactivation of the cellulase enzymes.
  • cellulases ar employed in liquid detergent compositions, it has typicall been necessary to protect them from proteolytic degradation.
  • the present invention relates to a detergent additiv comprising a cellulase and a protease, the protease being on which has a higher degree of specificity than Bacillus lentu serine protease.
  • the term "higher degree of specificity” is defined a a protease which conditions degrades human insulin to fewe components than does the B_j_ lentus serine protease under t following conditions: 0.5 ml of a 1 mg/ml solution of hum insulin in B and R buffer, pH 9.5, is incubated with 75 enzyme solution of 0.6 CPU (cf. Novo Nordis Analysis Meth No. AF 228/1) per litre for 120 min. at 37"C, and the reactio is quenched with 50 ⁇ l IN HCl.
  • the present invention relates to a detergen composition
  • a detergen composition comprising a cellulase and a protease as define above.
  • subtilisin Novo or a varian thereof (e.g. a variant described in US 4,914,031) , a proteas derivable from Nocardiopsis dassonvillei NRRL 18133 (describe in WO 88/03947) , a serine protease specific for glutamic an aspartic acid, producible by Bacillus licheniformis (thi protease is described in detail in co-pending Internationa patent application No. PCT/DK91/00067) , or a trypsin-lik protease producible by Fusarium sp., DSM 2672 (this proteas is described in WO 89/06270) .
  • the cellulase included in the detergent additive or detergent composition of the invention may be any enzyme exhibiting cellulolytic activity and may, for instance be derived from a strain of Humicola, Fusarium, Myceliophthora, Thermonospora, Bacillus or Strepto yces.
  • the cellulase is an endoglucanase, i.e. an enzyme which shows affinity for cellulose and which attacks amorphous regions of low crystallinity in cellulose fibres.
  • a particular endoglucanase which it has been found favourable to combine with a protease as defined above is is one which is immunoreactive with an antibody raised against a highly purified ⁇ 43 kD endoglucanase derived from Humicola insolens, DSM 1800.
  • One such enzyme which is decribed in detail in co- pending Danish patent application No. DK 1159/90 has the ami acid sequence shown in the appended Sequence Listing ID# 1. F the present purpose, this enzyme is referred to in t following as " ⁇ 43 kD endoglucanase".
  • the detergent additive according to the invention may suitab be in the form of a non-dusting granulate, stabilized liqu or protected enzyme.
  • Non-dusting granulates may be produc e.g. according to US 4,106,991 and 4,661,452 (both to No Industri A/S) and may optionally be coated by methods known the art.
  • Liquid enzyme preparations may, for instance, stabilized by adding a polyol such as propylene glycol, a sug or sugar alcohol, lactic acid or boric acid according established methods. Other enzyme stabilizers are well kno in the art.
  • Protected enzymes may be prepared according to t method disclosed in EP 238,216.
  • the detergent additive may suitably contain 0.001-1.0 mg cellulase and 0.001-1.0 mg of protease per gram of t additive. It will be understood that the detergent additive m further include one or more other enzymes, such as a lipas peroxidase or amylase, conventionally included in deterge additives.
  • the detergent composition of the invention additional comprises a surfactant which may be of the anionic, non-ioni cationic, amphoteric, or zwitterionic type as well as mixtur of these surfactant classes.
  • anion surfactants are linear alkyl benzene sulfonates (LAS) , alp olefin sulfonates (AOS) , alcohol ethoxy sulfates (AES) a alkali metal salts of natural fatty acids.
  • Detergent compositions of the invention may contain oth detergent ingredients known in the art as e.g. builders bleaching agents, bleach activators, anti-corrosion agents sequestering agents, anti soil-redeposition agents, perfumes enzyme stabilizers, etc.
  • the detergent composition of the invention may be formulate in any convenient form, e.g. as a powder or liquid.
  • the enzym may be stabilized in a liquid detergent by inclusion of enzym stabilizers as indicated above.
  • the pH of a solutio of the detergent composition of the invention will be 7-12 an in some instances 7.0-10.5.
  • Other detergent enzymes such a proteases.
  • Upases or amylases may be included the detergen compositions of the invention, either separately or in combined additive as described above.
  • the cellulase is typicall present in a concentration corresponding to 0.0005-20 mg o enzyme protein per litre of washing liquor, and the proteas is typically present in a concentration corresponding t 0.0005-20 mg per litre of washing liquor.
  • the detergen composition of the invention is usually employed i concentrations of 0.5 - 20 g/1 in the washing solution. I general, it is most convenient to add the detergent additiv in amounts of 0.1 - 5% w/w or, preferably, in amounts of 0.2 - 2% of the detergent composition.
  • the detergent additive or detergent composition of the invention may be used to reduce the rate at which cellulose- containing fabrics become harsh or to reduce the harshness of cellulose-containing fabrics, or to provide colour clarification of coloured cellulose-containing fabrics in a manner known per se (cf. for instance WO 89/00069) .
  • Endoglucanase 12 CMCU/g of detergent
  • CMCU CMCase activity
  • the CMCase activity was determined as follows:
  • a substrate solution of 30 g/1 CMC (Hercules 7 LFD) i deionized water was prepared.
  • the enzyme sample to b determined was dissolved in 0.01 M phosphate buffer, pH 7.5 1.0 ml of the enzyme solution and 2.0 ml of a 0.1 M phosphat buffer, pH 7.5, were mixed in a test tube, and an enzym reaction was initiated by adding 1.0 ml of the substrat solution to the test tube.
  • the mixture was incubated at 40" for 20 minutes, after which the reaction was stopped by addin 2.0 ml of 0.125 M trisodiu phosphate.12H 2 0.
  • a blind sample wa prepared without incubation.
  • a ferricyanide solution (1.60 g of potassiu ferricyanide and 14.0 g of trisodium phosphate.12H 2 0 in 11 o deionized water) was added to a test sample as well as to blind immediately followed by immersion in boiling water an incubation for 10 minutes. After incubation, the samples were cooled with tap water. The absorbance at 420 n was measured and a standard curve was prepared with glucose solution.
  • CMCase unit is defined as the amount of enzym which, under the conditions specified above, forms an amoun of reducing carbohydrates corresponding to 1 ⁇ mol of glucos per minute.
  • the storage stability of the endoglucanase of the invention was determined as its residual activity (in CMCU%) under the conditions indicated above.
  • TELECOMMUNICATION INFORMATION (A) TELEPHONE: +4544448888 (B) TELEF-.X: +4544493256 (C) TELEX: 37304
  • ORGANISM Humicola insolens

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Un additif à action détergente comprend une cellulase et une protéase dotées d'une spécificité supérieure à celle de la sérine-protéase de Bacillus lentus. En particulier la protéase peut être choisie parmi la subtilisine Novo ou l'une de ses variantes, une protéase pouvant être dérivée de Nocardiopsis dassonvillei NRRL 18133, une sérine-protéase spécifique aux acides glutamique et aspartique et pouvant être produite par Bacillus licheniformis, ou une protéase voisine de la trypsine pouvant être produite par Fusarium sp. DSM 2672.
PCT/DK1992/000116 1991-04-22 1992-04-10 Additif a action detergente renfermant une cellulase et une protease WO1992018599A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
BR9205921A BR9205921A (pt) 1991-04-22 1992-04-10 Aditivo detergente e composição detergente
EP92909626A EP0581839B1 (fr) 1991-04-22 1992-04-10 Additif a action detergente renfermant une cellulase et une protease
JP4508964A JPH06506712A (ja) 1991-04-22 1992-04-10 セルラーゼおよびプロテアーゼを含有する洗剤用添加剤
DE69212150T DE69212150T2 (de) 1991-04-22 1992-04-10 Cellulase und protease enthaltender reinigungsmittelzusatz
FI934656A FI934656A0 (fi) 1991-04-22 1993-10-21 Cellulase och proteas innehaollande tvaettmedel

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK737/91 1991-04-22
DK91737A DK73791D0 (da) 1991-04-22 1991-04-22 Detergentadditiv

Publications (1)

Publication Number Publication Date
WO1992018599A1 true WO1992018599A1 (fr) 1992-10-29

Family

ID=8096968

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1992/000116 WO1992018599A1 (fr) 1991-04-22 1992-04-10 Additif a action detergente renfermant une cellulase et une protease

Country Status (10)

Country Link
EP (1) EP0581839B1 (fr)
JP (1) JPH06506712A (fr)
AT (1) ATE140262T1 (fr)
BR (1) BR9205921A (fr)
DE (1) DE69212150T2 (fr)
DK (2) DK73791D0 (fr)
ES (1) ES2090636T3 (fr)
FI (1) FI934656A0 (fr)
MX (1) MX9201826A (fr)
WO (1) WO1992018599A1 (fr)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994016064A1 (fr) * 1993-01-18 1994-07-21 Novo Nordisk A/S Granulat contenant en enzyme, procede de production de ce granulat et son utilisation
WO1997020026A1 (fr) * 1995-11-27 1997-06-05 Unilever N.V. Compositions detergentes enzymatiques
WO1997020025A1 (fr) * 1995-11-27 1997-06-05 Unilever N.V. Compositions detergentes enzymatiques
US5707950A (en) * 1994-11-18 1998-01-13 The Procter & Gamble Company Detergent compositions containing lipase and protease
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
US6251144B1 (en) 1992-06-12 2001-06-26 Genencor International, Inc. Enzymatic compositions and methods for producing stonewashed look on indigo-dyed denim fabric and garments
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
WO2011003968A1 (fr) 2009-07-08 2011-01-13 Ab Enzymes Oy Protéase fongique et son utilisation
US8603795B2 (en) 2009-04-30 2013-12-10 Ab Enzymes Oy Fungal protease and use thereof
US8609390B2 (en) 2009-04-30 2013-12-17 Ab Enzymes Oy Fungal serine protease and use thereof
US8945900B2 (en) 2010-10-29 2015-02-03 Ab Enzymes Oy Variants of fungal serine protease
US9404164B2 (en) 2011-03-31 2016-08-02 Ab Enzymes Oy Protease enzyme and uses thereof
US12173261B2 (en) 2018-10-12 2024-12-24 Ab Enzymes Oy Protease enzyme variants and uses thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100491525C (zh) 2000-07-28 2009-05-27 汉高两合股份公司 从芽孢杆菌a7-7(dsm 12368)中提取的新型淀粉分解酶以及含有该新型淀粉分解酶的洗涤剂和清洗剂

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988003947A1 (fr) * 1986-11-25 1988-06-02 Novo Industri A/S Protease alcaline active a basse temperature obtenue a partir du nocardiopsis dassonvillei et preparation de ladite protease
WO1989009259A1 (fr) * 1988-03-24 1989-10-05 Novo-Nordisk A/S Preparation de cellulase
WO1991017243A1 (fr) * 1990-05-09 1991-11-14 Novo Nordisk A/S Preparation de cellulase comprenant un enzyme d'endoglucanase
WO1991019791A1 (fr) * 1990-06-15 1991-12-26 Novo Nordisk A/S Protease thermostable provenant de staphylothermus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988003947A1 (fr) * 1986-11-25 1988-06-02 Novo Industri A/S Protease alcaline active a basse temperature obtenue a partir du nocardiopsis dassonvillei et preparation de ladite protease
WO1989009259A1 (fr) * 1988-03-24 1989-10-05 Novo-Nordisk A/S Preparation de cellulase
WO1991017243A1 (fr) * 1990-05-09 1991-11-14 Novo Nordisk A/S Preparation de cellulase comprenant un enzyme d'endoglucanase
WO1991019791A1 (fr) * 1990-06-15 1991-12-26 Novo Nordisk A/S Protease thermostable provenant de staphylothermus

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Dialog Information Services, file 351, World Patent Index 81-90, Dialog Accession No. 004494826, (KAOS) KAO CORP), "Detergent compsn. with good washing effect on inorganic comprises standard detergent compsn. with protease and alkali cellulase"; & JP,A,60 226 600, 851111, 8551. *
Dialog Information Services, file 351, World Patent Index 81-90, Dialog Accession No. 004810933, (LION CORP), "Weakly, acidic detergent compsn. cont. cellulase alkali protease and acidic material"; & JP,A,61 231 094, 861015, 8648. *

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251144B1 (en) 1992-06-12 2001-06-26 Genencor International, Inc. Enzymatic compositions and methods for producing stonewashed look on indigo-dyed denim fabric and garments
WO1994016064A1 (fr) * 1993-01-18 1994-07-21 Novo Nordisk A/S Granulat contenant en enzyme, procede de production de ce granulat et son utilisation
US5707950A (en) * 1994-11-18 1998-01-13 The Procter & Gamble Company Detergent compositions containing lipase and protease
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
US7273748B2 (en) 1995-10-17 2007-09-25 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
US7323326B2 (en) 1995-10-17 2008-01-29 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
WO1997020026A1 (fr) * 1995-11-27 1997-06-05 Unilever N.V. Compositions detergentes enzymatiques
WO1997020025A1 (fr) * 1995-11-27 1997-06-05 Unilever N.V. Compositions detergentes enzymatiques
US5798327A (en) * 1995-11-27 1998-08-25 Lever Brothers Company Enzymatic detergent compositions
US8937170B2 (en) 2009-04-30 2015-01-20 Ab Enzymes Oy Nucleic acids encoding fungal protease
US8603795B2 (en) 2009-04-30 2013-12-10 Ab Enzymes Oy Fungal protease and use thereof
US8609390B2 (en) 2009-04-30 2013-12-17 Ab Enzymes Oy Fungal serine protease and use thereof
US8877912B2 (en) 2009-04-30 2014-11-04 Ab Enzymes Oy Nucleic acids encoding fungal serine protease
US8362222B2 (en) 2009-07-08 2013-01-29 Ab Enzymes Oy Fungal protease and use thereof
WO2011003968A1 (fr) 2009-07-08 2011-01-13 Ab Enzymes Oy Protéase fongique et son utilisation
US8945900B2 (en) 2010-10-29 2015-02-03 Ab Enzymes Oy Variants of fungal serine protease
US9404164B2 (en) 2011-03-31 2016-08-02 Ab Enzymes Oy Protease enzyme and uses thereof
US10221377B2 (en) 2011-03-31 2019-03-05 Ab Enzymes Oy Protease enzyme and uses thereof
US12173261B2 (en) 2018-10-12 2024-12-24 Ab Enzymes Oy Protease enzyme variants and uses thereof

Also Published As

Publication number Publication date
DK0581839T3 (da) 1996-10-28
DK73791D0 (da) 1991-04-22
ATE140262T1 (de) 1996-07-15
MX9201826A (es) 1992-10-01
EP0581839B1 (fr) 1996-07-10
DE69212150D1 (de) 1996-08-14
DE69212150T2 (de) 1997-02-06
FI934656L (fi) 1993-10-21
ES2090636T3 (es) 1996-10-16
BR9205921A (pt) 1994-07-05
FI934656A0 (fi) 1993-10-21
EP0581839A1 (fr) 1994-02-09
JPH06506712A (ja) 1994-07-28

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