+

WO1992017261A1 - Appareil de filtrage multiechantillon retenu par vide d'air et procede d'utilisation - Google Patents

Appareil de filtrage multiechantillon retenu par vide d'air et procede d'utilisation Download PDF

Info

Publication number
WO1992017261A1
WO1992017261A1 PCT/US1992/002752 US9202752W WO9217261A1 WO 1992017261 A1 WO1992017261 A1 WO 1992017261A1 US 9202752 W US9202752 W US 9202752W WO 9217261 A1 WO9217261 A1 WO 9217261A1
Authority
WO
WIPO (PCT)
Prior art keywords
vacuum
base plate
filter membrane
area
clamping
Prior art date
Application number
PCT/US1992/002752
Other languages
English (en)
Inventor
Patricia Crean Monti
Richard J. White
Michael K. Nicholson
Original Assignee
Nicholson Precision Instruments Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nicholson Precision Instruments Inc. filed Critical Nicholson Precision Instruments Inc.
Priority to JP4510638A priority Critical patent/JPH06506869A/ja
Publication of WO1992017261A1 publication Critical patent/WO1992017261A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G01N33/5304—Reaction vessels, e.g. agglutination plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates

Definitions

  • This invention relates generally to an apparatus and method thereof for biochemical testing and screening. More particularly, the present invention relates to a multi-sample filtration apparatus and method thereof for bio edical testing and screening of multiple samples.
  • Multi-sample, filtration apparatus are generally used for sampling all types of media.
  • the testing of extracts of blood, extracts of cells or purified nucleic acids from a variety of sources is a common application of the apparatus.
  • the testing of extracts of blood, whole cells or purified materials are common applications.
  • Conventional apparatus typically operate by allowing a sample to come into contact with a filter membrane. Tests are then performed on the membrane, and a variety of determinations can be made regarding the sample media.
  • Conventional filtration devices further allow for testing of multiple samples, so as to allow more than one type of media or multiple samples of identical media to be tested.
  • Fernwood Patent One type of multi-sample filtration apparatus, disclosed in United States Patent No. 4,493,815 to Fernwood et aJL (referred to herein as the "Fernwood Patent") employs a vacuum member to draw the media into contact with a filter membrane. The purpose of the vacuum member is to bring the media in contact with the filter membrane.
  • the Fernwood Patent also discloses a plurality of mechanical screws for "sandwiching" (clamping) the assembly together. Clamping of the assembly is necessary to prevent migration of samples on the membrane and leakage of vacuum.
  • Apparatus such as that disclosed by the Fernwood Patent, however, have several disadvantages.
  • One disadvantage relates to sealing. If a good seal is not obtained, samples will migrate across the filter membrane causing serious problems when a lab technician is trying to analyze the membrane. Some samples will be destroyed, others will be placed in a condition that will not readily facilitate analysis by the technician.
  • the present invention is directed to overcoming one or more of the problems set forth above.
  • the present invention is an apparatus for testing sample media using a filter membrane.
  • the apparatus first comprises a well plate configured in a standard 96 well microtiter configuration. Each well is adapted to contain the sample media.
  • the apparatus further comprises a first means for vacuum-clamping the filter membrane in sealable contact with the well plate.
  • the vacuum clamping means seals each of the wells of the well plate in contact with the filter membrane.
  • the apparatus further comprises a second means for drawing the sample media contained in each well of the well plate into contact with the filter membrane.
  • the vacuum clamping means comprises a vacuum clamping valve and a first vacuum inlet mounted on a base plate.
  • the vacuum inlet is adapted to connect with an external vacuum source.
  • the vacuum clamping valve is rotatable to a first position where the vacuum clamping valve is in an "on” position and a second position where the vacuum clamping valve is in an "off” position.
  • the first vacuum inlet is in communication with a second channel to supply a vacuum to a vacuum clamping region of the apparatus.
  • the vacuum clamping means may further comprise a gasket.
  • the gasket has first and second substantially parallel elastomeric surfaces and a plurality of wells in registration with wells of the well plate.
  • the base plate has formed therein a vacuum reservoir surrounded by a plurality of islands.
  • the gasket is mounted about the islands of the base plate to form the vacuum clamping area.
  • the vacuum clamping feature of the present invention provides several advantages heretofore unavailable in conventional media testing apparatus.
  • One such advantage relates to the resultant sample media on the filter membrane.
  • the vacuum clamping feature provides a more consistent clamping force than that of the screws and clamps used in conventional apparatus.
  • a consistent clamping force ensures that each of the sample media will be properly placed on the filter membrane.
  • a second advantage relates to operation. More particularly, the vacuum clamping feature makes operation of the present invention significantly easier than conventional apparatus. Ease of operation leads to increased production output and efficiency.
  • Figure 1 is a exploded view of the present invention
  • Figure 2A is a perspective view of the base plate
  • Figure 2B is a sectional view of the base plate
  • Figure 3 is a cut-away view of the valve
  • Figure 4A is a perspective view of the gaske ;
  • Figure 4B is a sectional view of the gasket
  • Figure 5A is a plan view of the well plate
  • Figure 5B is a sectional view of the well plate.
  • the present invention is an apparatus configured to sample media.
  • One feature of the present invention is a first vacuum member adapted to bring the media in contact with a filter membrane.
  • a second feature of the present invention is a second vacuum member adapted to provide the clamping force necessary to seal the apparatus so that the first vacuum member can properly bring the media in contact with the filter membrane.
  • the vacuum clamping feature of the present invention provides several advantages heretofore unavailable in conventional media testing apparatus.
  • One such advantage relates to the resultant sample media on the filter membrane.
  • the vacuum clamping feature provides a more consistent clamping force than that of the screws and clamps used in conventional apparatus.
  • a consistent clamping force ensures that each of the sample media will be properly placed on the filter membrane.
  • a second advantage relates to operation. More particularly, the vacuum clamping feature makes operation of the present invention significantly easier than conventional apparatus. Ease of operation leads to increased production output and efficiency.
  • the apparatus 100 generally comprises a filter membrane 110, a base plate 112, a first vacuum valve 114, a second vacuum valve 116, a gasket 118, a well plate 120, and a cover plate 122.
  • the filter membrane 110 is provided to receive and capture sample media.
  • the filter membrane 110 is well known in the art.
  • filter membrane 110 is made of either nylon or nitrocellulose.
  • any type of filter membrane could potentially be used with the present invention.
  • Such alternative filter membranes include, but are not limited to, polyvinylidene fluoride membranes, cellulose acetate membranes, and modified nylon membranes.
  • the base plate 112 is generally configured to provide the two independent vacuum features.
  • the first vacuum feature operates to bring the sample media contained in well plate 120 into contact with the filter membrane 110.
  • the second vacuum feature operates to clamp the filter membrane 110, base plate 112, gasket 118, and well plate 120 assembly together.
  • the second vacuum feature replaces the screw clamps found in conventional apparatus.
  • the first vacuum valve 114 is generally provided to regulate the first vacuum feature. As will be more fully described herein, the first vacuum valve 114 is configured to allow the user to control the rate at which the media samples are drawn into contact with the filter membrane 110. As shown by cover plate 122, the first vacuum valve 114 is rotatable to a first position where the vacuum valve 114 is in an "on" position 134 and a second position where the first vacuum valve 114 is in an "off" position 136. When the first vacuum valve 114 is in the "on" position 134, a vacuum is generated in the base plate 112 causing the sample media in the well plate 120 to be drawn into contact with the filter membrane 110.
  • the second vacuum valve 116 is generally provided to regulate the second vacuum feature. As will be more fully described herein, the second vacuum valve 116, allows the user to control the clamp force that holds the filter membrane 110, base plate 112, gasket 118, and well plate 120 assembly together. Applica ⁇ tion of the clamp force allows the apparatus 100 to be quickly assembled. Removal of the clamp force allows the apparatus 100 to be quickly disassembled. As also shown by the cover plate 122, the second vacuum valve 116 is rotatable to a first position where the second vacuum valve 116 is in an "on" position 130 and a second position where the second vacuum valve 116 is in a "release" position 132. When the second vacuum valve 116 is in the "on" position 130, a vacuum is generated in the base plate 112 causing the base plate 112/gasket 118/filter membrane 110/well plate 120 assembly to be securely held together.
  • the gasket 118 is generally provided to seal the apparatus 100 such that the first vacuum feature and the second vacuum feature can operate at optimum performance levels. As will be shown more fully herein, gasket 118 ensures that the necessary clamping force is maintained such that no leakage of vacuum occurs from the second vacuum feature during operation of the first vacuum feature. Prevention of vacuum leakage from the second vacuum feature during operation of the first vacuum feature is essential to ensuring that the sample media is properly disposed on the filter membrane 110. Failure to prevent vacuum leakage from the second vacuum feature may result in migration of sample media across the filter membrane 110.
  • the well plate 120 is generally provided to hold the sample media to be tested by the apparatus 100. As will be described more fully herein, well plate 120 may be configured in a conventional 96 well microtiter configuration thereby allowing multiple sample media to be tested.
  • Base plate 112 comprises a first vacuum inlet 206 and a second vacuum inlet 208.
  • First vacuum inlet 206 and second vacuum inlet 208 enable an external vacuum source (not shown) to be connected to the apparatus 100.
  • First vacuum inlet 206 corresponds to first vac ⁇ um feature.
  • Second vacuum inlet 208 corresponds to the second vacuum feature.
  • First vacuum inlet 206 and second vacuum inlet 208 are mounted via holes (not shown) in the base plate 112 by conventional mounting means.
  • first vacuum inlet 206 and second vacuum inlet 208 are quick disconnect hose barb fittings. The only important criteria is that each of vacuum inlets 206,208 be capable of mating with the conventional tubing which is typically connected to the external vacuum source.
  • first vacuum inlet 206 and second vacuum inlet 208 could be connected to the same vacuum source.
  • a wide variety of vacuum sources may be used with the apparatus 100.
  • Base plate 112 further comprises a first opening 210 and a first channel 212.
  • First opening 210 provides communication between the first vacuum inlet 206 and the first channel 212.
  • First opening 210 is configured to accept the first vacuum valve 114.
  • rotation of the first vacuum valve 114 causes the first vacuum inlet 206 to be in and/or out of registration with the first channel 212.
  • the first channel 212 extends into a reservoir area 250 (to be described) .
  • Base plate 112 further comprises a second opening 214 and second channel 216.
  • Second opening 214 is in communication with the second vacuum inlet 208 and the second channel 216.
  • Second opening 214 is configured to accept the second vacuum valve 116.
  • rotation of the second vacuum valve 116 causes the second vacuum inlet 208 to be in and/or out of registration with the second channel 216.
  • the second channel 216 extends into a vacuum clamping area 255 (shown in Figure 4A and to be described in conjunction therewith) .
  • the vacuum reservoir area 250 is a recessed region within the base plate 112.
  • the vacuum reservoir area 250 acts as a receptacle wherein a vacuum is generated to draw the sample media from the well plate 120 in contact with the filter membrane 110.
  • the reservoir area 250 is sized so as to provide a proper vacuum area and to drain the sample media which passes through the filter membrane 110.
  • the vacuum reservoir area 250 may take a variety of configurations.
  • the vacuum reservoir area 250 has formed therein a plurality of channels 252 equidistantly arranged.
  • a single channel 254 connects the plurality of channels 252 to each other.
  • Single channel 254 is provided so as to connect the channels 252 together.
  • the plurality of channels 252 that are formed in the reservoir area 250 are in registration with the openings (to be described) in the gasket 118 and the wells (to be described) of the well plate 120.
  • the base plate 112 has further formed therein a plurality of islands 275. As will be discussed below, the islands 275 in combination with the gasket 118 form the vacuum clamping area 255.
  • the vacuum clamping area 255 is hereby defined as the area where the vacuum clamping means operates on the well plate 120.
  • the islands 275 are sized to closely receive the gasket 118.
  • a gasket recess 270 is formed within the base plate 112, such that the top portion of gasket 118 (to be described) is at substantially the same height with the islands 275 of the base plate 112.
  • the base plate 112 further comprises an opening 280 positioned at the top of one island 275. Opening 280 is in communication with the second channel 216. Opening 280 introduces the clamping vacuum to the top of the island 275.
  • the well plate 120 has formed a vacuum transfer channel (to be described) which allows the vacuum present at the opening 280 to uniformly disperse throughout the top of the remaining islands 275 and those portions of the gasket 118 that lie between the islands 275.
  • the base plate 112 may further comprise a pair of positioning pins 290.
  • the positioning pins 290 enable the well plate 120 to be accurately placed on top of the base plate 112 and gasket 118 when the apparatus 100 is assembled.
  • first vacuum valve 114 operates as a switch to turn on and off the sampling vacuum. In particular, rotation of the first vacuum valve 114 causes the first vacuum inlet 206 to be in and out of registration with the first channel 212.
  • First vacuum valve 114 has an upper portion 302 and a lower portion 304. The lower portion 304 fits closely into the opening 210 of the base plate 112. A close fit is required to minimize leakage of vacuum. However, the lower portion 304 should be allowed to rotate within the opening 210.
  • first channel 306 Formed in the lower portion 304 is a first channel 306 and a second channel 308.
  • Channel 306 has at one end a first opening 318 and at the second end a second opening 316.
  • Channel 308 extends completely through the center of the lower portion 304.
  • Channel 308 has at one end a first opening 310 and at the second end a second opening 312.
  • the second opening 316 of first channel 306 intersects with the second channel 308 thus allowing communication between the first channel 306 and the second channel 308.
  • the lower portion is further designed such that when the openings 310 and 312 are in registration with the first vacuum inlet 206 and first channel 212 of the base plate 112, respectively, the external vacuum source (not shown) is in communication with the vacuum reservoir area 250, and thus is turned “on.” When the openings 310 and 312 are not in registration with the first vacuum inlet 206 and first channel 212 of the base plate 112, the sampling vacuum is turned “off.”
  • the lower portion is further designed such that when the opening 318 is in registration with a third channel 235 of the base plate 112, the opening 310 is in registration with the first channel 212, thus providing communication between the ambient atmosphere and the vacuum reservoir area 250. This communication allows the user to pressurize the vacuum reservoir area 250.
  • the first vacuum valve 114 further has formed ridge 320.
  • Ridge 320 is configured such that it will rest on the surface of the cover plate 122. Ridge 320 ensures that the openings 310 and 312 will register with the vacuum inlet 206 and first channel 212 and that openings 316 and 318 will register with third channel 235 and first channel 212.
  • the second vacuum valve 116 is designed in a manner similar to that of first vacuum valve 114 described above.
  • the second vacuum valve 116 is designed such that rotation of the second vacuum valve 116 to a first (i.e., "on") position results in communication between second vacuum inlet 208 and second channel 216 of base plate 112, thereby providing a vacuum to the vacuum clamping area 255.
  • Rotation of the second vacuum valve 116 to a second position results in communication between a fourth channel 237 and the second channel 216 of the base plate 112, thus allowing for pressurization of the vacuum clamping area 255.
  • gasket 118 is made out of a flexible material, such as silicon or the like. In order to perform repeated filtrations with the same gasket, its material composition must be resilient. However, gasket 118 can be made out of a variety of flexible and/or resilient materials.
  • the gasket 118 first comprises an inner section 402.
  • Inner section 402 has formed therein a plurality of wells 404.
  • wells 404 In the embodiment shown by Figure 4, a standard 96 microtiter well configuration is depicted.
  • the claimed invention anticipates the use of any number of wells taking a variety of shapes and sizes.
  • the gasket 118 further comprises a circular ridge 420 (not shown in Figure 4A) surrounding each well 404.
  • Each circular ridge 420 rises approximately .002 inches above the surface of each well 404.
  • Each circular ridge 420 ensures, upon assembly of apparatus 100 that an airtight seal is made with the corresponding well (to be described) of the well plate 120.
  • the gasket 118 further comprises a webbed section 406.
  • Webbed section 406 comprises a plurality of webs 414 and bridges 416. The webs 414 and bridges 416 are configured to mate with the islands 275 formed in the base plate 112 to thereby firmly secure the gasket 118 therein.
  • the gasket 118 further comprises a first border ridge 410 and a second border ridge 412.
  • First border ridge 410 surrounds the perimeter of the inner area 402.
  • Second border ridge 412 surrounds the perimeter of the webbed section 406.
  • the first and second border ridges 410 and 412 are about .002 inch in height and upon assembly, function to define a closed vacuum clamping area 255 that will not leak vacuum.
  • the gasket 118 further comprises a tab portion 418.
  • Tab portion 418 is provided to allow easy removal of the gasket 118 from the base plate 112.
  • Tab portion 418 is configured to mate with recess 272 of the base plate 112.
  • Well plate 120 first comprises a plurality of wells 502.
  • the wells 502 are configured in a standard 96 well microtiter configuration.
  • well plate 120 could have wells of a variety of configuration depending on the type of test to be run in the apparatus 100.
  • the well plate 120 further comprises a recessed vacuum channel 504 extending around the perimeter of the well 502 pattern. Upon assembly, the area at the periphery of the vacuum channel 504 would be in sealable contact with the ridges 410 and 412 of the gasket 118. As such, the vacuum channel 504 becomes the ceiling for the vacuum clamping area 255 and closes it.
  • the well plate 120 further comprises a pair of pin insets 506, 508 to facilitate the placement of the well plate 120 with the base plate 112. Upon assembly, the positioning pins 290 of the base plate 112 mate with the pin insets 506 and 508, respectively.
  • the first vacuum valve 114 is set to the "release” position and the second vacuum valve 116 is set to the "off” position. As such, operation of the apparatus 100 can now begin.
  • the external vacuum source is assumed to be on.
  • the gasket 118 is first placed onto the base plate 112.
  • the plurality of webs 414 and bridges 416 mate with the corresponding islands 275 on the base plate.
  • the filter membrane 110 is placed on the inner section 402 of the gasket 118.
  • the well plate 120 is placed on the base plate 112 via mating pins 290 and insets 506 and 508.
  • the wells 502 of the well plate 120 are in substantial registration with the wells 404 of the gasket 118.
  • the channels 252 of base plate 112 are positioned directly under the wells 404 of the gasket 118.
  • the apparatus 100 is ready to be clamped into position.
  • the second vacuum valve 116 is rotated to the "on" position.
  • the well plate 120 is forced into contact with the upper surface of the islands 275 and the ridges 410 and 412 of the gasket 118.
  • the gasket 118 conforms with any irregularities found on the surface of well plate 120.
  • the circular ridges surrounding the wells 502 of the gasket 118 are also compressed, thereby providing complete separation between the individual wells 502 of the well plate 120 on the filter membrane 110. Complete isolation of the wells 502 on the membrane 110 eliminates bleeding or migration of sample media across the filter membrane 110.
  • the vacuum clamping force reaches maximum and the apparatus 100 is fully clamped.
  • the ridges 410 and 412 of the gasket 118 are in sealable contact with the well plate 120 and the vacuum clamping area 255 is fully enclosed by the islands 275, the ridges 410 and 412, and the vacuum channel 504 of the well plate 120.
  • Sample media may be loaded into the wells 502 of the well plate 120. Thereafter, the filtration process is initiated by rotating the first vacuum valve 114 to the "on” position. Rotation of the first vacuum valve 114 to the "on” position causes a vacuum to come about in the vacuum reservoir area 250. As such, the sample media contained in the wells 502 of the well plate 120 are brought into contact with the filter membrane 110. Sample media that passes through the filter membrane 110 is discharged through the vacuum reservoir area 250 and out the channel 212 and first vacuum inlet 206.
  • the user Upon completion of the filtration process, the user rotates the first vacuum valve 114 to the "off" position. In the “off” position, external vacuum source is disengaged from the vacuum reservoir area
  • the vacuum reservoir area 250 is in communication with the surrounding ambient environment. This pressurizes the vacuum reservoir area 250 thereby stopping the drainage of sample media from the wells 502 of the well plate 120.
  • the user has two options. First, the user can re-load the well plate 120 with additional and/or different sample media. Because the second vacuum valve 116 is still in the "on" position, the user can not remove the well plate 120 and as such any re-loading of sample media would have to be done with the well plate 120 still in place.
  • the user may want to remove the filter membrane 110 for further testing or the user may want to remove only the well plate 120 and replace it with an additional well plate 120 containing additional and/or different sample media to be tested with the existing sample media filtrate which is already present on the filter membrane 110.
  • the user has to rotate the second vacuum valve 116 to the "release” position. Rotation of the second vacuum valve 116 to the "release” position causes the external vacuum source to be disengaged from the vacuum clamping area 255 and the vacuum clamping area 255 to be in communication with the surrounding ambient environment. As such, this pressurizes the vacuum clamping area 255 thereby releasing the clamping force holding the apparatus 100 together.
  • the well plate 120 can be removed and replaced with another well plate 120 without removing the filter membrane 110.
  • the user can remove the well plate 120 and the filter membrane 110.
  • the filter membrane 110 can than be further tested as desired.
  • the gasket 118 can be removed by lifting up the tab 418 on gasket 118.
  • the gasket 118 can then be cleaned and replaced on the base plate 112 for use in the next test.
  • a different gasket 118 can be placed thereon.
  • the present invention can draw the sample media into contact with the filter membrane in an uncontaminated manner.
  • the vacuum clamping feature of the present invention acts to isolate each well pattern on the filter membrane thereby eliminating sample media migration or so called bleeding that occurs in conventional apparatus.
  • the apparatus of the present invention can be quickly assembled and disassembled thus increasing test production output and efficiency.
  • Such modifications and/or alternative embodiment may include, but are not limited to, an additional channel formed in the well plate 120 so as to allow the space surrounding the annular ridge 420 of each well 404 in gasket 118 to be in communication with the ambient environment. This would guard against evacuation of this area in the event of an imperfect performance of the first border ridge 410 once vacuum clamp is applied and in effect. Vacuum in this area would increase the likelihood of cross-well migration of samples.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Appareil servant à filtrer plusieurs échantillons biologiques à l'aide d'une membrane de filtre (110). L'appareil (100) comprend une plaque de base (112), une plaque de cuvettes (120), un joint hermétique (118), et un premier élément à vide d'air (114) permettant de maintenir ensemble par effet de vide d'air la plaque de base (112), la plaque de cuvettes (120), le joint hermétique (118) et la membrane de filtre (110). L'appareil peut comprendre en outre un deuxième élément à vide d'air (116) qui aspire les échantillons biologiques de la plaque de cuvettes (120) pour qu'ils viennent en contact avec la membrane de filtre (110).
PCT/US1992/002752 1991-04-04 1992-04-06 Appareil de filtrage multiechantillon retenu par vide d'air et procede d'utilisation WO1992017261A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4510638A JPH06506869A (ja) 1991-04-04 1992-04-06 真空クランプ多数サンプル濾過装置および方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68054491A 1991-04-04 1991-04-04
US680,544 1991-04-04

Publications (1)

Publication Number Publication Date
WO1992017261A1 true WO1992017261A1 (fr) 1992-10-15

Family

ID=24731540

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1992/002752 WO1992017261A1 (fr) 1991-04-04 1992-04-06 Appareil de filtrage multiechantillon retenu par vide d'air et procede d'utilisation

Country Status (3)

Country Link
EP (1) EP0582638A1 (fr)
JP (1) JPH06506869A (fr)
WO (1) WO1992017261A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994028110A1 (fr) * 1993-06-01 1994-12-08 Celsis International Plc Dispositif de detection
CN109590994A (zh) * 2019-01-09 2019-04-09 安徽明天氢能科技股份有限公司 一种用于阴阳极板上线的机械手
CN110636904A (zh) * 2017-06-08 2019-12-31 因特格拉生物科学有限公司 具有防真空功能的样品和试剂容器

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7824623B2 (en) 2003-06-24 2010-11-02 Millipore Corporation Multifunctional vacuum manifold

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4427415A (en) * 1979-01-05 1984-01-24 Cleveland Patrick H Manifold vacuum biochemical test method and device
US4493815A (en) * 1983-07-28 1985-01-15 Bio-Rad Laboratories, Inc. Supporting and filtering biochemical test plate assembly
US4797259A (en) * 1986-12-15 1989-01-10 Pall Corporation Well-type diagnostic plate device
US4895706A (en) * 1986-10-28 1990-01-23 Costar Corporation Multi-well filter strip and composite assemblies
US4927604A (en) * 1988-12-05 1990-05-22 Costar Corporation Multiwell filter plate vacuum manifold assembly

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2176601B (en) * 1985-06-10 1989-02-01 Bio Rad Laboratories Test plate assembly defining discrete regions on a microporous membrane with low boundary distortion
US4902481A (en) * 1987-12-11 1990-02-20 Millipore Corporation Multi-well filtration test apparatus

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4427415A (en) * 1979-01-05 1984-01-24 Cleveland Patrick H Manifold vacuum biochemical test method and device
US4493815A (en) * 1983-07-28 1985-01-15 Bio-Rad Laboratories, Inc. Supporting and filtering biochemical test plate assembly
US4895706A (en) * 1986-10-28 1990-01-23 Costar Corporation Multi-well filter strip and composite assemblies
US4797259A (en) * 1986-12-15 1989-01-10 Pall Corporation Well-type diagnostic plate device
US4927604A (en) * 1988-12-05 1990-05-22 Costar Corporation Multiwell filter plate vacuum manifold assembly

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP0582638A4 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994028110A1 (fr) * 1993-06-01 1994-12-08 Celsis International Plc Dispositif de detection
AU668652B2 (en) * 1993-06-01 1996-05-09 Celsis International Plc Detection apparatus
US5811257A (en) * 1993-06-01 1998-09-22 Celsis International, P.L.C. Detection apparatus
CN110636904A (zh) * 2017-06-08 2019-12-31 因特格拉生物科学有限公司 具有防真空功能的样品和试剂容器
CN109590994A (zh) * 2019-01-09 2019-04-09 安徽明天氢能科技股份有限公司 一种用于阴阳极板上线的机械手
CN109590994B (zh) * 2019-01-09 2023-08-22 安徽明天氢能科技股份有限公司 一种用于阴阳极板上线的机械手

Also Published As

Publication number Publication date
EP0582638A1 (fr) 1994-02-16
EP0582638A4 (fr) 1994-03-23
JPH06506869A (ja) 1994-08-04

Similar Documents

Publication Publication Date Title
US5227137A (en) Vacuum clamped multi-sample filtration apparatus
US5108603A (en) Self-contained vacuum clamped multi-sample media filtration apparatus and method
US4948442A (en) Method of making a multiwell test plate
US5047215A (en) Multiwell test plate
CA2043633C (fr) Plateau-filtreur multi-echantillons
CA1210309A (fr) Plaque d'essai biochimique a double usage
EP1383604B1 (fr) Mat d'etancheite a commande d'ecoulement unidirectionnel pour plaque a puits
US5205989A (en) Multi-well filtration apparatus
US5516490A (en) Apparatus for preventing cross-contamination of multi-well test plates
WO1999000655A3 (fr) Appareil et procede d'essai de liaison rapide en ecoulement continu
CA1286578C (fr) Plaque d'essi comprenant une membrane poreuse sur laquelle des regions sont definies de maniere qu'il y ait peu de distorsion de leurs limites
AU2003210528A1 (en) Unidirectional flow control sealing matt for well plate
JP2007263966A (ja) マルチウェルマイクロ濾過装置
JPH02149321A (ja) フィルタ組立体
CN111065446B (zh) 用于微生物测试的过滤组件和方法
US5409832A (en) Membrane holder for use in an assay device
US20210245106A1 (en) Filtration assembly and method for microbiological testing
EP0582638A4 (fr)
WO1996039250A1 (fr) Dispositif de filtration pour echantillons multiples
JP4794582B2 (ja) 多機能真空マニフォールド
US6149871A (en) System for processing multiple specimens of biological fluid
EP0986435A1 (fr) Adaptateur de volume pour plaque multi-puits
US20050103703A1 (en) Method of assembling a filtration plate
EP0075405A1 (fr) Unité de filtration réutilisable avec membranes récupérables
CA2125159A1 (fr) Cartouche d'analyse

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): CA JP

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU MC NL SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1992910414

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1992910414

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1992910414

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: CA

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载