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WO1992011760A1 - Procede et recipient de conservation d'organismes animaux - Google Patents

Procede et recipient de conservation d'organismes animaux Download PDF

Info

Publication number
WO1992011760A1
WO1992011760A1 PCT/IT1992/000001 IT9200001W WO9211760A1 WO 1992011760 A1 WO1992011760 A1 WO 1992011760A1 IT 9200001 W IT9200001 W IT 9200001W WO 9211760 A1 WO9211760 A1 WO 9211760A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
conservation
larvae
bedding
air
Prior art date
Application number
PCT/IT1992/000001
Other languages
English (en)
Inventor
Enzo Silingardi
Original Assignee
Silingardi, Andrea
Silingardi, Matteo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Silingardi, Andrea, Silingardi, Matteo filed Critical Silingardi, Andrea
Publication of WO1992011760A1 publication Critical patent/WO1992011760A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K97/00—Accessories for angling
    • A01K97/04—Containers for bait; Preparation of bait
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
    • A01N1/146—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/16—Physical preservation processes

Definitions

  • the invention concerns a method for the conservation of animal organisms for use in fishing, in laboratories, for biological pest control in agriculture, the container and packaging relating thereto, that is to say a new process for the conservation, as well as fishing bait such as flesh fly larvae belonging to the genuses Sarcophaga and Calliphora ucilia, of other small animal organisms, for example worms such as Polycheta and Oligocheta for use as bait and Hyrodinea for laboratory use, and also of insects such as Coleopters, Coccinellids, Dipterans, Syrphids, Neuropters, Chrysopids, Hemiptera, Anthocorids, predatory mites (in particular Phytoseidites, Stigmeites, Thrombididites) , all suitable for the destruction of harmful insects and microorganisms: the container and packaging of the product also forming the object of the request for protection.
  • fishing bait such as flesh fly larvae belonging to the genuses Sarcophaga and Calliphora u
  • ccnssrvaticn in a sealed container on a bed of non- absorbent insulating powder considered suitable to prevent dehydration, in a percentage amount of approximately 5% by weight with respect to the larvae and made up of 60% ground corn-cob, 37% of ground polyethylene, plus powdered mint leaves to make up 100%.
  • the container the following was provided:
  • auxiliary holes in a screen stuck to the cover consisting of a film provided with holes having a diameter of between 1 and 2 mm for extraction, by means of sifting, of the insulating powder and for re-animation of the larvae, after their period of reduced activity, before gradual removal of the screen itself.
  • the invention solves the above technical problem by using a method which provides for the conservation of the animal organism, to be used as fishing bait, or for biological pest control, or for laboratory uses, immobilized by an anoxic environment, that is to say caused by the absence of oxygen in the environment into which it is put, with the following conditions: container with rarefied atmosphere, preferably at approximately 0,8 bars, or the successive creation of a.
  • inert gasses preferably carbon dioxide, or nitrogen, or a mixture thereof, up to atmospheric pressure
  • - container containing bedding preferably between 10 and 15% by weight with respect to that of the animal organisms contained therein, to prevent on the one hand excessive dehydration, and on the other hand excessive humidity: said bedding being made up as follows: absorbent granular material (without dust to avoid abrasion of the cuticle) such as montmorillonite, zeolite, chalk, kaolin, mucilage, or mixtures thereof 25% disinfectant (for example sorbic acid and its salts, benzole acid and its salts, propionic acid) 5% natural aromas or bacteria chosen to perfume and deodorize 2-10% inert filling, preferably sawdust, or wood shavings, or bran, or corn-cob or polyester shavings, or textile trimmings, or mixtures thereof, or other materials that do not interact with the animal organisms to make 100%
  • - container impermeable to light - temperature within the container preferably of 2° C with a tolerance limit of up to 8-10° C and with the possibility of exposure at even up to 15 - 20° C : in the latter case only for a period of 10-20 hours; as regards the container, the following are provided: - material with low light-permeability in the single container or, at least, in the packaging surrounding one or more containers;
  • - bags for example in plastic material, suitable for extraction of air and the successive possible substitution by inert gasses of the air extracted;
  • - box-like or tray-like containers in plastic material, plastic-coated cardboard, cardboard with internal coating in aluminium or other metal or impermeable and non- reactive paint, suitable for the operations indicated above;
  • - box-shaped, or tray-shaped metal containers in tin- plate, aluminium, or other metal, internally coated, for example with non-reactive paints, again on condition that said containers are suitable for the operations listed above.
  • the box-shaped container must be able to be joined, on the moment of opening, to a perforated lid allowing air exchange between the inside and the outside: the holes in said lid must not be equal to or exceeding the minimum dimensions of the animal organism and of the grains of bedding, for example, in the case of flesh fly larvae, said holes must not exceed a maximum width of 2 mm, in order to avoid escape of said larvae and of the granular bedding.
  • the packing can be multi-layer or single layer: the former, indicated for the storage, transport and commercialization of large quantities, to the detriment of the recovery time of the organisms, is also indicated, for example, for the operations of chumming preliminary to fishing, where rapid return to activity of the larvae is not required; the latter, indicated when the return to activity of the organism must, on the contrary, be practically immediate, as, for example, in the case of larvae used for angling, to the detriment of the weight of each single package, which cannot exceed approximately 200 g without having basic dimensions which would in practice be inconvenient.
  • the volume of the animal organism-bedding mixture in relation to that of the box-like container, in the case of evacuation of the air, given the low degree of vacuum, this will be almost the same as the geometrical volume of the container.
  • the advantages obtained by this invention are the following: complete interruption of motorial activity in the animal organisms for extremely long periods, for example about thirty days with zero mortality, and up to even forty days or more with a mortality rate which increases from zero to less than 10-15% overall from the thirtieth day onwards, in the case of larvae for use in fishing; recovery of motorial activity in an extremely brief space of time when compared with the conservation period, even for long conservation periods; large increase in survival rates and weight conservation; no need for manipulation from the moment of closure of the container until the re-opening of the same; an economical process; increased utilization of volume; suspension of the metamorphosis from larva into pupa, in the case of flesh fly larvae, for the whole of the conservation period, in the practical absence of oxygen; inhibition of the damaging development of microorganisms within the container; duration of the residual life of the animal organism equal to that which it would have had without anoxic treatment; possibility of application of the method, of the container and of the packing, to worms, to insects for use in biological pest control and
  • the bedding according to the present invention when compared with that used in the preceding invention by the same applicant: - gives complete elimination of the humidity produced by the larvae during the brief period lasting from closure of the package until their immobilization, thus preventing the growth and development of harmful microorganisms, the putrefaction of the larvae due to long periods of contact with the humidity, the giving off of bad smells in spite of the presence of the deodorant and the negative effect of presenting the customer with larvae in said state;
  • EXAMPLE i 80 bags in opaque polyethylene were prepared, each one containing 50 g of larvae in a multi-layer arrangement and 5 g of bedding according to the invention; half of them were closed after rarefaction to 0,8 bars, while the other half, after rarefaction, was first brought back to 1 bar by introduction of a gaseous mixture formed by 25% carbon dioxide and 75% nitrogen.
  • each bag On the opening of each bag the following was invariably found. Up to and including the 29th day, and in both types of bag: larvae dry, absence of bad smells, zero mortality rate. From the 30th day a slight mortality began, showing an increase of approximately 1% per day for the bags under depression and of 1-2% per day for the bags under controlled atmosphere.
  • EXAMPLE 4 40 bags in opaque polyethylene were prepared, each containing 50 g of flesh fly larvae, with 7,5 g of bedding according to the invention, the whole in a single layer, with rarefaction to 0,8 bars, temperature + 2° C, thermometrically regulated; upon opening of each bag it was invariably found that the larvae were sweet smelling and there was no humidity.
  • the test was above all aimed at ascertaining the time required for recovery of motorial activity during forty days, opening one bag every 24 hours: much shorter recovery times were found than those given in Table 1, the 35 minutes of the first day were reduced to as little as 3 minutes, and, respectively, 470-410 minutes to only 60 minutes.
  • the mortality rate a slight decrease was noted between the 29th and the 35th day (see curve D in the diagrams of Figures 1 and 2) .
  • Figure 1 is a diagram showing percentage mortality, as a function of time, for the flesh fly larvae, as found during tests performed under a variety of conditions, the abscissa showing the duration of the test in days, and the ordinate showing the percentage mortality rate found: in said diagram A, B, C and D, respectively, indicate the curves relating to a conservation test at a temperature of 18° C and to three tests carried out at a thermostatically controlled temperature of 2° C: curve D relating to a mono-layer package;
  • Figure 2 shows, on a larger scale, the development of the diagram relating to tests B, C and D performed according to the invention, shown in a compressed manner in Figure 1;
  • Figure 3 is a perspective view of a rigid box-shaped container, the upper surface of which, open before introduction of the larvae, is formed by a hermetically sealed film, said box being provided with a perforated lid for application during use: it is put into
  • Figure 6 is a perspective view of a possible container with flexible walls, of the bag kind, packed in a multi-layer arrangement, in its depressed condition;
  • Figure 7 is a partial transversal cross-section of the container according to Figure 6;
  • Figure 8 is a cross- section like that shown in Figure 7, but showing a flexible, single layer container.
  • the rigid container for example made of plastic material, containing the larvae, or the animal organisms in general 2, mixed with bedding material 3 » , closed under depression (for example at a pressure of 0,8 bars) by means of flexible film 3, heat- sealed, liquid- and gas-tight, along the edge 4 of the container 1 and provided with tear-away tongues 5; with 6 a lid for reanimation which snaps onto the edge 4, said lid being provided with perforations 7 having a diameter preferably not exceeding approximately 2 mm, set at a distance p of approximately 1 cm in both directions; with 8 a flexible container, for example in polyethylene, for multi-layer packaging; with 9 a similar container, but for single-layer packaging.
  • the packaging process in the case of flesh fly larvae, takes place in the following manner: the larvae 2, born approximately one week before, that is to say at the end of the nutrition period of their larval phase, are collected, sawdust is added, the whole is sieved and kept in open boxes at a lowest nominal temperature of 2° C, thermostatically controlled, for a preparation period of approximately 24 hours, during which time the motorial activity slows down by even as much as 60-70%; having been extracted from the refrigerator, they are mixed with bedding- 3' in a container and transferred to the dosing hopper on the packing machine, which performs the following operations:
  • the packages are transferred to a refrigerator having a lowest temperature of 2° C (obviously oscillating up to 8- 10° C), from this moment on the packages can be delivered to the seller - even without treatment using cooling means - for transportation and up to the end of the fourth day from closing: after which it is necessary to replace them in a refrigerator (again at a minimum of 2° C with a variation of temperature of up to 8-10° C caused by the thermostat) for at least six hours before use.
  • the larvae can be advantageously used after as much as forty or more days, the only obstacle being an increased mortality rate of over 10-15%.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Feed For Specific Animals (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)

Abstract

Procédé de conservation d'organismes animaux du type pouvant servir d'appâts de pêche, aux recherches en laboratoire ou à la lutte contre les parasites biologiques. Cette conservation est nécessaire pour permettre aux organismes de sortir indemnes des périodes particulièrement longues (allant jusqu'à 30 ou 40 jours) de transport ou analogue sans perte sensible de qualité ou de valeur, lesdits organismes demeurant intacts et dans des conditions aptes à permettre une reprise rapide de leur activité. Ledit procédé consiste principalement à introduire les organismes (2) dans un récipient rigide (1) ou souple (8, 9) contenant une litière spéciale (3'), puis à raréfier l'air dans le récipient, de préférence jusqu'à 0,8 bars environ, et éventuellement à remplacer l'air lui-même par des gaz inertes, de manière à arrêter l'activité et le développement. Le procédé peut servir, par exemple, à la conservation des larves de sarcophages destinées à servir d'appâts pour la pêche de loisirs.
PCT/IT1992/000001 1991-01-03 1992-01-03 Procede et recipient de conservation d'organismes animaux WO1992011760A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITMO910003A IT1246018B (it) 1991-01-03 1991-01-03 Metodo per la conservazione di organismi animali utilizzati nella pesca, in laboratorio, nella lotta biologica in agricoltura: contenitore e confezionamento relativi.
ITMO91A000003 1991-01-03

Publications (1)

Publication Number Publication Date
WO1992011760A1 true WO1992011760A1 (fr) 1992-07-23

Family

ID=11384836

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT1992/000001 WO1992011760A1 (fr) 1991-01-03 1992-01-03 Procede et recipient de conservation d'organismes animaux

Country Status (3)

Country Link
AU (1) AU1171692A (fr)
IT (1) IT1246018B (fr)
WO (1) WO1992011760A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995026633A3 (fr) * 1994-03-31 1995-11-23 Dikla International Substrat de ponte pour les insectes
WO1999044416A1 (fr) * 1998-03-06 1999-09-10 Joaquin Rodriguez Sanchez Emballage de vers marins vivants
WO2000074478A1 (fr) * 1999-06-08 2000-12-14 Wilhelm Fleischmann Procede et dispositif de culture d'insectes destines notamment a obtenir la secretion de larves de mouches aux fins d'application therapeutique
GB2422315A (en) * 2004-11-20 2006-07-26 Zoobiotic Ltd Maggot delivery system
EP2070410A1 (fr) * 2007-12-13 2009-06-17 Milestone S.r.l. Processus de préservation de tissus
US8821896B2 (en) 2009-01-16 2014-09-02 Sanaria Inc. Purified Plasmodium and vaccine composition
US9278125B2 (en) 2011-05-11 2016-03-08 Sanaria Inc. Pharmaceutical compositions comprising attenuated Plasmodium sporozoites and glycolipid adjuvants
EP3000746A1 (fr) * 2014-09-23 2016-03-30 Willy Worns Ltd Récipient et procédé d'utilisation de ceux-ci
US9764016B2 (en) 2013-01-25 2017-09-19 Sanaria Inc. Genetic attenuation of Plasmodium by B9 gene disruption

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117972357B (zh) * 2024-03-26 2024-06-07 山东科瑞特自动化装备有限责任公司 一种水位测量装置的水位监测数据智能处理方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1071041B (it) * 1980-05-05 1985-04-02 Individuale Silingardi Enzo Metodo per la conservazione di larve di mosca e contenitore relativo

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1071041B (it) * 1980-05-05 1985-04-02 Individuale Silingardi Enzo Metodo per la conservazione di larve di mosca e contenitore relativo

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHEMICAL PATENTS INDEX, BASIC ABSTRACTS JOURNAL Section Ch, Week 8535, Derwent Publications Ltd., London, GB; Class C, AN 212340 & IT,B,1 071 041 (SILINGARDI) 2 April 1985 cited in the application *

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995026633A3 (fr) * 1994-03-31 1995-11-23 Dikla International Substrat de ponte pour les insectes
WO1999044416A1 (fr) * 1998-03-06 1999-09-10 Joaquin Rodriguez Sanchez Emballage de vers marins vivants
ES2134176A1 (es) * 1998-03-06 1999-09-16 Rodriguez Sanchez Joaquin Embalaje para gusanos de mar vivos.
WO2000074478A1 (fr) * 1999-06-08 2000-12-14 Wilhelm Fleischmann Procede et dispositif de culture d'insectes destines notamment a obtenir la secretion de larves de mouches aux fins d'application therapeutique
US6557487B1 (en) * 1999-06-08 2003-05-06 Wilhelm Fleischmann Method and device for rearing insects, especially for obtaining a secretion from fly larvae for therapeutic application
AU777660B2 (en) * 1999-06-08 2004-10-28 Wilhelm Fleischmann Method and device for rearing insects, especially for obtaining a secretion from fly larvae for therapeutic application
US6863022B2 (en) 1999-06-08 2005-03-08 Wilhelm Fleischmann Method and device for rearing insects, especially for obtaining secretion from fly larvae for therapeutic application
KR100571946B1 (ko) * 1999-06-08 2006-04-18 빌헬름 플라이쉬만 치료용의 파리 유충 분비물을 얻기 위한 곤충 사육 방법 및 이를 위한 장치
GB2422315B (en) * 2004-11-20 2009-11-11 Zoobiotic Ltd Maggot delivery system
GB2422315A (en) * 2004-11-20 2006-07-26 Zoobiotic Ltd Maggot delivery system
US8110346B2 (en) 2007-12-13 2012-02-07 Milestone S.R.L. Process for preserving tissues
EP2070410A1 (fr) * 2007-12-13 2009-06-17 Milestone S.r.l. Processus de préservation de tissus
US9616115B2 (en) 2009-01-16 2017-04-11 Sanaria Inc. Purified plasmodium and vaccine compositions
US8821896B2 (en) 2009-01-16 2014-09-02 Sanaria Inc. Purified Plasmodium and vaccine composition
US8992944B2 (en) 2009-01-16 2015-03-31 Sanaria Inc. Purified Plasmodium and vaccine composition
US9241982B2 (en) 2009-01-16 2016-01-26 Sanaria Inc. Purified plasmodium and vaccine compositions
US10272146B2 (en) 2009-01-16 2019-04-30 Sanaria Inc. Purified plasmodium and vaccine compositions
US9278125B2 (en) 2011-05-11 2016-03-08 Sanaria Inc. Pharmaceutical compositions comprising attenuated Plasmodium sporozoites and glycolipid adjuvants
US9642909B2 (en) 2011-05-11 2017-05-09 Sanaria Inc. Pharmaceutical compositions comprising attenuated Plasmodium sporozoites and glycolipid adjuvants
US9764016B2 (en) 2013-01-25 2017-09-19 Sanaria Inc. Genetic attenuation of Plasmodium by B9 gene disruption
US9931389B2 (en) 2013-01-25 2018-04-03 Sanaria Inc. Genetic attenuation of plasmodium by B9 gene disruption
EP3000746A1 (fr) * 2014-09-23 2016-03-30 Willy Worns Ltd Récipient et procédé d'utilisation de ceux-ci

Also Published As

Publication number Publication date
AU1171692A (en) 1992-08-17
ITMO910003A0 (it) 1991-01-03
IT1246018B (it) 1994-11-07
ITMO910003A1 (it) 1992-07-03

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