+

WO1992011375A1 - Modification de la pomme de terre par manipulation genetique permettant la formation de fecule du type amylose - Google Patents

Modification de la pomme de terre par manipulation genetique permettant la formation de fecule du type amylose Download PDF

Info

Publication number
WO1992011375A1
WO1992011375A1 PCT/SE1991/000891 SE9100891W WO9211375A1 WO 1992011375 A1 WO1992011375 A1 WO 1992011375A1 SE 9100891 W SE9100891 W SE 9100891W WO 9211375 A1 WO9211375 A1 WO 9211375A1
Authority
WO
WIPO (PCT)
Prior art keywords
potato
starch
gene
amylose
antisense
Prior art date
Application number
PCT/SE1991/000891
Other languages
English (en)
Inventor
Per Hofvander
Per T. Persson
Anneli Tallberg
Olle Wikström
Original Assignee
Amylogene Hb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amylogene Hb filed Critical Amylogene Hb
Priority to PL91299927A priority Critical patent/PL169859B1/pl
Publication of WO1992011375A1 publication Critical patent/WO1992011375A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Definitions

  • the present invention relates to genetically engi- neered modification of potato, resulting in the formation of an increasing amount of amylose-type starch as compared to amylopectin-type starch in the potato.
  • the genetically engineered modification implies the insertion of a gene fragment into potato, said gene fragment comprising tran- scription start and a part of the gene coding for the for ⁇ mation of branching enzyme (BE gene) in potato, inserted in the antisense direction, together with a tuber-specific promoter.
  • BE gene branching enzyme
  • starch In the potato tuber, starch is the greatest part of the solid matter. About 1/4 to 1/5 of the starch in potato is amylose, while the remainder of the starch is amylo- pectin. These two components of the starch have different fields of application, and therefore the possibility of producing either pure amylose or pure amylopectin is most interesting.
  • the two starch components can be produced from common starch, which requires a number of process steps and, consequently, is expensive and complicated.
  • BE branching enzyme
  • Branching enzyme (BE) in potato is a monomer protein, i.e. it is a single enzyme form.
  • the molecular weight of potato BE varies between 79 and 103 kD, depending on the purifying process used. There are indications that potato BE should consist of several forms, but presumably several forms are degradation products from the actual protein (Vos-Scheperkeuter, 1989; Blennow & Johansson, 1990).
  • the invention comprises a method of sup ⁇ pressing formation of amylopectin-type starch in potato, whereby the potato tubers form a varyingly increased amount of amylose-type starch.
  • amylose Physical modification of the amylose can be effected by e.g. cylinder-drying or extrusion.
  • Example 5 Chemical derivatisation of amylose Amylose is sludged in water to a concentration of 20-50% by weight. The pH is adjusted to 5.0-12.0, and sodium hypochlorite is added so that the end product obtains the desired viscosity. The reaction temperature is set at 20-45°C. When the reaction is completed, the pH is adjusted to 4-8, whereupon the end product is washed and dried. In this manner, oxidised starch is obtained.
  • Example 6 Physical derivatisation of amylose
  • SEQ ID No. 1 Sequenced molecule: genomic DNA Name: Promoter for the GBSS gene from potato Length of sequence: 629 bp

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Modification de la pomme de terre par manipulation génétique permettant d'inhiber la formation de la fécule du type amylopectine. On décrit un produit de synthèse anti-sens destiné à inhiber jusqu'à un degré variable l'expression du gène codant pour la formation de l'enzyme d'embranchement (gène BE) dans les pommes de terre, ledit produit anti-sens comportant un promoteur spécifique des tubercules, un initiateur de transcription et le premier exon du gène BE, introduits dans le sens anti-sens. On décrit également des cellules, des plantes, des tubercules, des microtubercules et des graines de pomme de terre comportant ledit produit de synthèse anti-sens. Enfin, on décrit la fécule du type amylose, à la fois non dénaturée et dérivée, dérivée des pommes de terre manipulées génétiquement, ainsi qu'un procédé d'inhibition de la formation d'amylopectine dans les pommes de terre.
PCT/SE1991/000891 1990-12-21 1991-12-20 Modification de la pomme de terre par manipulation genetique permettant la formation de fecule du type amylose WO1992011375A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL91299927A PL169859B1 (pl) 1990-12-21 1991-12-20 Sposób hamowania tworzenia sie w ziemniakach skrobi typu amylopektyny PL

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9004095A SE9004095L (sv) 1990-12-21 1990-12-21 Genetisk foeraendring av potatis foer bildning av staerkelse av amylostyp
SE9004095-7 1990-12-21

Publications (1)

Publication Number Publication Date
WO1992011375A1 true WO1992011375A1 (fr) 1992-07-09

Family

ID=20381266

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1991/000891 WO1992011375A1 (fr) 1990-12-21 1991-12-20 Modification de la pomme de terre par manipulation genetique permettant la formation de fecule du type amylose

Country Status (5)

Country Link
EP (1) EP0563201A1 (fr)
AU (1) AU9109791A (fr)
PL (1) PL169859B1 (fr)
SE (1) SE9004095L (fr)
WO (1) WO1992011375A1 (fr)

Cited By (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994011520A3 (fr) * 1992-11-09 1994-08-04 Zeneca Ltd Nouvelles plantes et procedes de production
WO1995007355A1 (fr) * 1993-09-09 1995-03-16 Institut Für Genbiologische Forschung Berlin Gmbh Combinaison de sequences d'adn permettant la formation d'amidon modifie dans des cellules vegetales et des vegetaux, procedes de production de ces plantes et de l'amidon modifie obtenu a partir de ladite combinaison
WO1995026407A1 (fr) * 1994-03-25 1995-10-05 National Starch And Chemical Investment Holding Corporation Procede pour produire une fecule modifiee a partir de plants de pommes de terre
WO1995031553A1 (fr) * 1994-05-18 1995-11-23 Institut Für Genbiologische Forschung Berlin Gmbh SEQUENCES D'ADN CODANT DES ENZYMES SUSCEPTIBLES DE FACILITER LA SYNTHESE D'α-1,4 GLYCANNES LINEAIRES CHEZ LES VEGETAUX, LES CHAMPIGNONS ET LES MICRO-ORGANISMES
WO1996034968A3 (fr) * 1995-05-05 1996-12-05 Nat Starch Chem Invest Ameliorations apportees a une composition a base d'amidon de plante
WO1997011188A1 (fr) * 1995-09-19 1997-03-27 Planttec Biotechnologie Gmbh Plantes synthetisant un amidon modifie, procede de production de telles plantes, et amidon modifie obtenu a partir de ces plantes
WO1997004112A3 (fr) * 1995-07-14 1997-05-15 Danisco Inhibition de l'expression genetique
WO1997004113A3 (fr) * 1995-07-14 1997-05-15 Danisco Inhibition de l'expression genetique
EP0584809B1 (fr) * 1992-08-28 1997-12-29 National Starch and Chemical Investment Holding Corporation Amidon à basse teneur zu amylopectine
WO1998020145A3 (fr) * 1996-11-05 1998-07-16 Nat Starch Chem Invest Ameliorations dans la teneur de plantes en amidon ou la concernant
WO1998037213A1 (fr) * 1997-02-21 1998-08-27 Danisco A/S Inhibition de l'expression de l'enzyme ramifiante de l'amidon par un intron antisens
US5908975A (en) * 1993-11-09 1999-06-01 E. I. Du Pont De Nemours And Company Accumulation of fructans in plants by targeted expression of bacterial levansucrase
US5998701A (en) * 1997-06-04 1999-12-07 Her Majesty The Queen In Right Of Canada As Represented By The Department Of Agriculture Potatoes having improved quality characteristics and methods for their production
DE19836098A1 (de) * 1998-07-31 2000-02-03 Hoechst Schering Agrevo Gmbh Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke
US6066782A (en) * 1993-09-09 2000-05-23 Hoechst Schering Agrevo Gmbh Combination of DNA sequences which enable the formation of modified starch in plant cells and plants, processes for the production of these plants and the modified starch obtainable therefrom
US6143562A (en) * 1995-04-06 2000-11-07 Seminis Vegetable Seeds Carbon-based process for selection of transgenic plant cells
EP1103617A3 (fr) * 1991-02-13 2001-11-28 Aventis CropScience GmbH Plasmides contenant des séquences d'ADN provoquant des changements dans la concentration et la composition glucidiques de plantes, cellules de plantes et plantes contenant ces plasmides
US6521816B1 (en) 1998-11-09 2003-02-18 Planttec Biotechnologie Gmbh Forschung Und Entwicklung Nucleic acid molecules from rice and their use for the production of modified starch
WO2003054024A1 (fr) * 2001-12-21 2003-07-03 Bayer Cropscience Gmbh Amidons pregelatinises et procedes de production associes
WO2003078629A1 (fr) 2002-03-20 2003-09-25 Basf Plant Science Gmbh Produit de synthese et procede de regulation de l'expression genique
WO2004005516A1 (fr) * 2002-07-09 2004-01-15 Basf Plant Science Gmbh Utilisation de genes mutants ahas comme marqueurs de selection dans la transformation des pommes de terre
US7012171B2 (en) 1989-12-21 2006-03-14 Advanced Technologies Cambridge Limited Modification of plant metabolism
US7186898B1 (en) 1996-12-19 2007-03-06 Bayer Bioscience Gmbh Nucleic acid molecules from maize and their use for the production of modified starch
EP2267138A2 (fr) 1998-04-08 2010-12-29 Commonwealth Scientific and Industrial Research Organization Procédés et moyens d'obtention de phénotypes modifiés
US8598332B1 (en) 1998-04-08 2013-12-03 Bayer Cropscience N.V. Methods and means for obtaining modified phenotypes
WO2013184768A1 (fr) 2012-06-05 2013-12-12 University Of Georgia Research Foundation, Inc. Compositions et méthodes d'inactivation génique dans les plantes
US9029527B2 (en) 1998-03-20 2015-05-12 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs
EP2980220A1 (fr) 2005-09-20 2016-02-03 BASF Plant Science GmbH Procédés améliorés de contrôle de l'expression de gènes
US9441239B2 (en) 1998-04-08 2016-09-13 Commonwealth Scientific & Industrial Research Organisation Methods and means for obtaining modified phenotypes
US9708621B2 (en) 1999-08-13 2017-07-18 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US9963698B2 (en) 1998-03-20 2018-05-08 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
CN114703208A (zh) * 2022-01-21 2022-07-05 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) 马铃薯StGAPC基因在提高马铃薯淀粉含量上的用途

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE513209C2 (sv) 1995-11-29 2000-07-31 Lars Rask Förfarande för produktion av transgena potatisar med ökad eller minskad grad av förgrening av amylopektinstärkelse

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0335451A2 (fr) * 1988-03-25 1989-10-04 Vereniging Voor Christelijk Wetenschappelijk Onderwijs Cellules végétales et plantes produites par génie génétique ainsi que l'ADN recombinant approprié
EP0368506A2 (fr) * 1988-11-10 1990-05-16 Zeneca Limited ADP glucose-pyrophosphorylase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0335451A2 (fr) * 1988-03-25 1989-10-04 Vereniging Voor Christelijk Wetenschappelijk Onderwijs Cellules végétales et plantes produites par génie génétique ainsi que l'ADN recombinant approprié
EP0368506A2 (fr) * 1988-11-10 1990-05-16 Zeneca Limited ADP glucose-pyrophosphorylase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BLENNOW A., JOHANSSON G.: "ISOLATION OF A Q-ENZYME WITH M, 103000 FROM POTATO TUBERS.", PHYTOCHEMISTRY, PERGAMON PRESS, GB, vol. 30., no. 02., 1 January 1991 (1991-01-01), GB, pages 437 - 444., XP000617730, ISSN: 0031-9422, DOI: 10.1016/0031-9422(91)83700-U *
VOS-SCHEPERKEUTER G. H., ET AL.: "IMMUNOLOGICAL COMPARISON OF THE STARCH BRANCHING ENZYMES FROM POTATO TUBERS AND MAIZE KERNELS.", PLANT PHYSIOLOGY., AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD., US, vol. 90., 1 January 1989 (1989-01-01), US, pages 75 - 84., XP000617628, ISSN: 0032-0889 *

Cited By (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7012171B2 (en) 1989-12-21 2006-03-14 Advanced Technologies Cambridge Limited Modification of plant metabolism
EP1103617A3 (fr) * 1991-02-13 2001-11-28 Aventis CropScience GmbH Plasmides contenant des séquences d'ADN provoquant des changements dans la concentration et la composition glucidiques de plantes, cellules de plantes et plantes contenant ces plasmides
EP0584809B1 (fr) * 1992-08-28 1997-12-29 National Starch and Chemical Investment Holding Corporation Amidon à basse teneur zu amylopectine
WO1994011520A3 (fr) * 1992-11-09 1994-08-04 Zeneca Ltd Nouvelles plantes et procedes de production
US6066782A (en) * 1993-09-09 2000-05-23 Hoechst Schering Agrevo Gmbh Combination of DNA sequences which enable the formation of modified starch in plant cells and plants, processes for the production of these plants and the modified starch obtainable therefrom
WO1995007355A1 (fr) * 1993-09-09 1995-03-16 Institut Für Genbiologische Forschung Berlin Gmbh Combinaison de sequences d'adn permettant la formation d'amidon modifie dans des cellules vegetales et des vegetaux, procedes de production de ces plantes et de l'amidon modifie obtenu a partir de ladite combinaison
US5908975A (en) * 1993-11-09 1999-06-01 E. I. Du Pont De Nemours And Company Accumulation of fructans in plants by targeted expression of bacterial levansucrase
WO1995026407A1 (fr) * 1994-03-25 1995-10-05 National Starch And Chemical Investment Holding Corporation Procede pour produire une fecule modifiee a partir de plants de pommes de terre
US6103893A (en) * 1994-03-25 2000-08-15 National Starch And Chemical Investment Holding Corporation High amylose starch from transgenic potato plants
US6818421B2 (en) 1994-05-18 2004-11-16 Bayer Bioscience Gmbh DNA sequences coding for enzymes capable of facilitating the synthesis of linear α-1,4 glucans in plants, fungi and microorganisms
WO1995031553A1 (fr) * 1994-05-18 1995-11-23 Institut Für Genbiologische Forschung Berlin Gmbh SEQUENCES D'ADN CODANT DES ENZYMES SUSCEPTIBLES DE FACILITER LA SYNTHESE D'α-1,4 GLYCANNES LINEAIRES CHEZ LES VEGETAUX, LES CHAMPIGNONS ET LES MICRO-ORGANISMES
US6265635B1 (en) 1994-05-18 2001-07-24 Plantec Biotechnologie Gmbh Forschung & Entwicklung DNA sequences coding for enzymes capable of facilitating the synthesis of linear α-1,4 glucans in plants, fungi and microorganisms
US6143562A (en) * 1995-04-06 2000-11-07 Seminis Vegetable Seeds Carbon-based process for selection of transgenic plant cells
US6974894B2 (en) 1995-05-05 2005-12-13 National Starch And Chemical Company Plant Starch Composition
WO1996034968A3 (fr) * 1995-05-05 1996-12-05 Nat Starch Chem Invest Ameliorations apportees a une composition a base d'amidon de plante
US6825342B1 (en) 1995-05-05 2004-11-30 National Starch And Chemical Investment Holding Corporation Plant starch composition
WO1997004113A3 (fr) * 1995-07-14 1997-05-15 Danisco Inhibition de l'expression genetique
AU709798B2 (en) * 1995-07-14 1999-09-09 Dupont Nutrition Biosciences Aps Inhibition of gene expression
WO1997004112A3 (fr) * 1995-07-14 1997-05-15 Danisco Inhibition de l'expression genetique
US6232122B1 (en) 1995-07-14 2001-05-15 Danisco A/S Inhibition of gene expression
US6147279A (en) * 1995-07-14 2000-11-14 Danisco A/S Inhibition of gene expression
EP1435205A1 (fr) * 1995-09-19 2004-07-07 Bayer BioScience GmbH Plantes synthetisant un amidon modifie, procede de production de telles plantes,et amidon modifie obtenu a partir de ces plantes
EP1728441A3 (fr) * 1995-09-19 2007-03-07 Bayer BioScience GmbH Plantes synthétisant un amidon modifié, procédé de production de telles plantes, et amidon modifié obtenu à partir de ces plantes
US6207880B1 (en) 1995-09-19 2001-03-27 Planttec Biotechnologie Gmbh Plants which synthesize a modified starch, process for the production thereof and modified starch
AU715944B2 (en) * 1995-09-19 2000-02-10 Bayer Cropscience Aktiengesellschaft Plants which synthesize a modified starch, process for the production thereof and modified starch
US8586722B2 (en) 1995-09-19 2013-11-19 Bayer Cropscience Ag Methods of using tubers having genetically modified potato plant cells
US7897760B2 (en) 1995-09-19 2011-03-01 Bayer Bioscience Gmbh Plants which synthesize a modified starch, process for the production thereof and modified starch
US7569744B2 (en) 1995-09-19 2009-08-04 Bayer Bioscience Gmbh Plants which synthesize a modified starch, process for the production thereof and modified starch
US7176190B2 (en) 1995-09-19 2007-02-13 Bayer Bioscience Gmbh Plants which synthesize a modified starch, process for the production thereof and modified starch
EP1702518A1 (fr) * 1995-09-19 2006-09-20 Bayer BioScience GmbH Plantes synthétisant un amidon modifié, procédé de production de telles plantes, et amidon modifié obtenu à partir de ces plantes
WO1997011188A1 (fr) * 1995-09-19 1997-03-27 Planttec Biotechnologie Gmbh Plantes synthetisant un amidon modifie, procede de production de telles plantes, et amidon modifie obtenu a partir de ces plantes
US6815581B2 (en) 1995-09-19 2004-11-09 PlantTec Biotechnologies, GmbH Plants which synthesize a modified starch, process for the production thereof and modified starch
US6956148B1 (en) * 1996-11-05 2005-10-18 National Starch And Chemical Investment Holding Corporation Nucleic acids from cassava encoding starch branching enzyme II (SBEII) and their use
WO1998020145A3 (fr) * 1996-11-05 1998-07-16 Nat Starch Chem Invest Ameliorations dans la teneur de plantes en amidon ou la concernant
GB2320716B (en) * 1996-11-05 2001-06-20 Nat Starch Chem Invest Improvements in or relating to starch content of plants
US7186898B1 (en) 1996-12-19 2007-03-06 Bayer Bioscience Gmbh Nucleic acid molecules from maize and their use for the production of modified starch
WO1998037213A1 (fr) * 1997-02-21 1998-08-27 Danisco A/S Inhibition de l'expression de l'enzyme ramifiante de l'amidon par un intron antisens
US6483009B1 (en) 1997-02-21 2002-11-19 Danisco A/S Antisense intron inhibition of starch branching enzyme expression
US5998701A (en) * 1997-06-04 1999-12-07 Her Majesty The Queen In Right Of Canada As Represented By The Department Of Agriculture Potatoes having improved quality characteristics and methods for their production
US9963698B2 (en) 1998-03-20 2018-05-08 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
US9029527B2 (en) 1998-03-20 2015-05-12 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs
US8598332B1 (en) 1998-04-08 2013-12-03 Bayer Cropscience N.V. Methods and means for obtaining modified phenotypes
US9441239B2 (en) 1998-04-08 2016-09-13 Commonwealth Scientific & Industrial Research Organisation Methods and means for obtaining modified phenotypes
EP2267138A2 (fr) 1998-04-08 2010-12-29 Commonwealth Scientific and Industrial Research Organization Procédés et moyens d'obtention de phénotypes modifiés
EP2267139A2 (fr) 1998-04-08 2010-12-29 Commonwealth Scientific and Industrial Research Organization Procédés ét moyens d'obtention de phénotypes modifies
EP3214177A2 (fr) 1998-04-08 2017-09-06 Commonwealth Scientific and Industrial Research Organisation Procédés et moyens pour obtenir des phénotypes modifiés
US6596928B1 (en) 1998-07-31 2003-07-22 Bayer Cropscience Gmbh Plants synthesizing a modified starch, the generation of the plants, their use, and the modified starch
US7247769B2 (en) 1998-07-31 2007-07-24 Bayer Cropscience Gmbh Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
US7385104B2 (en) 1998-07-31 2008-06-10 Bayer Cropscience Ag Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
DE19836098A1 (de) * 1998-07-31 2000-02-03 Hoechst Schering Agrevo Gmbh Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke
US7449623B2 (en) 1998-11-09 2008-11-11 Bayer Bioscience Gmbh Nucleic acid molecules from rice and their use for the production of modified starch
US6521816B1 (en) 1998-11-09 2003-02-18 Planttec Biotechnologie Gmbh Forschung Und Entwicklung Nucleic acid molecules from rice and their use for the production of modified starch
US9708621B2 (en) 1999-08-13 2017-07-18 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US10190127B2 (en) 1999-08-13 2019-01-29 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US7045003B2 (en) 2001-12-21 2006-05-16 Bayer Cropscience Gmbh Pregelatinized starches and processes for their production
WO2003054024A1 (fr) * 2001-12-21 2003-07-03 Bayer Cropscience Gmbh Amidons pregelatinises et procedes de production associes
CN100348617C (zh) * 2001-12-21 2007-11-14 拜尔作物科学有限责任公司 预胶凝化淀粉和它们的生产方法
WO2003078629A1 (fr) 2002-03-20 2003-09-25 Basf Plant Science Gmbh Produit de synthese et procede de regulation de l'expression genique
EP2202311A2 (fr) 2002-07-09 2010-06-30 BASF Plant Science GmbH Utilisation de gènes mutés AHAS en tant que marqueur de sélection dans la transformation de la pomme de terre
EP2292768A1 (fr) 2002-07-09 2011-03-09 BASF Plant Science GmbH Utilisation des gènes mutés AHAS comme marqueurs de sélection chez la transformation de la pomme de terre
EP2202311A3 (fr) * 2002-07-09 2010-07-28 BASF Plant Science GmbH Utilisation des gènes mutés AHAS comme marqueurs de sélection chez la transformation de la pomme de terre
WO2004005516A1 (fr) * 2002-07-09 2004-01-15 Basf Plant Science Gmbh Utilisation de genes mutants ahas comme marqueurs de selection dans la transformation des pommes de terre
EP2980220A1 (fr) 2005-09-20 2016-02-03 BASF Plant Science GmbH Procédés améliorés de contrôle de l'expression de gènes
WO2013184768A1 (fr) 2012-06-05 2013-12-12 University Of Georgia Research Foundation, Inc. Compositions et méthodes d'inactivation génique dans les plantes
CN114703208A (zh) * 2022-01-21 2022-07-05 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) 马铃薯StGAPC基因在提高马铃薯淀粉含量上的用途

Also Published As

Publication number Publication date
SE467160B (sv) 1992-06-01
SE9004095L (sv) 1992-06-01
EP0563201A1 (fr) 1993-10-06
AU9109791A (en) 1992-07-22
SE9004095D0 (sv) 1990-12-21
PL169859B1 (pl) 1996-09-30

Similar Documents

Publication Publication Date Title
WO1992011375A1 (fr) Modification de la pomme de terre par manipulation genetique permettant la formation de fecule du type amylose
US5824798A (en) Genetically engineered modification of potato to obtain amylopectin-type starch
JP4148964B2 (ja) デンプン合成に関わる酵素をコードするｄｎａ分子、ならびに該ｄｎａ分子を含むベクター、細菌、トランスジェニック植物細胞およびトランスジェニック植物体
US6066782A (en) Combination of DNA sequences which enable the formation of modified starch in plant cells and plants, processes for the production of these plants and the modified starch obtainable therefrom
US6570066B1 (en) Nucleotide sequences encoding enzymes that alter the carbohydrate concentration and composition in plants
CA2331300C (fr) Ameliorations apportees a des plantes et a des produits vegetaux
EP0826061B1 (fr) Ameliorations apportees a une composition a base d'amidon de plante
KR100540824B1 (ko) 감자의 전분 분지형성 효소 ⅱ
WO1999012950A9 (fr) Perfectionnements se rapportant a la stabilite des amidons des vegetaux
US5856467A (en) Genetically engineered modification of potato to form amylose-type starch

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AT AU BB BG BR CA CH DE DK ES FI GB HU JP KP KR LK LU MG MW NL NO PL RO SD SE SU US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE BF BJ CF CG CH CI CM DE DK ES FR GA GB GN GR IT LU MC ML MR NL SE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 1992901887

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1992901887

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA

WWR Wipo information: refused in national office

Ref document number: 1992901887

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1992901887

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载