+

WO1992005415A1 - Indicateur de temperature-temps - Google Patents

Indicateur de temperature-temps Download PDF

Info

Publication number
WO1992005415A1
WO1992005415A1 PCT/GB1991/001538 GB9101538W WO9205415A1 WO 1992005415 A1 WO1992005415 A1 WO 1992005415A1 GB 9101538 W GB9101538 W GB 9101538W WO 9205415 A1 WO9205415 A1 WO 9205415A1
Authority
WO
WIPO (PCT)
Prior art keywords
substrate
enzyme
indicator according
reaction
label
Prior art date
Application number
PCT/GB1991/001538
Other languages
English (en)
Inventor
David John Livesley
Original Assignee
Cambridge Consultants Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Consultants Limited filed Critical Cambridge Consultants Limited
Publication of WO1992005415A1 publication Critical patent/WO1992005415A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
    • G01K3/00—Thermometers giving results other than momentary value of temperature
    • G01K3/02—Thermometers giving results other than momentary value of temperature giving means values; giving integrated values
    • G01K3/04—Thermometers giving results other than momentary value of temperature giving means values; giving integrated values in respect of time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives

Definitions

  • This invention concerns time temperature indication and relates to a time temperature indicator, more especially for chilled or frozen foods.
  • the invention also relates to a label incorporating such an indicator, and a method of time temperature indication.
  • a time temperature indicator is a device for indicating temperature conditions with respect to time. In the case of chilled or frozen food products, for example, it is important to be able to establish if a product has been subject to proper temperature conditions over a period of time, since this determines the shelf life of the product.
  • One time temperature indicator is known from U.K. Specification No. 1366797.
  • the principle of this indicator is an enzyme catalysed reaction. Enzyme catalysed reaction rates are affected by a number of parameters, but given a standard chemical environment, the major parameter is temperature. Enzymes have an optimum temperature range at which they operate, and below that range reaction rates are considerably slowed.
  • the relationship between temperature and reaction rate may be plotted and enzymes selected for which the relationship of reaction rate to temperature over the temperature range of 4-20 C is linear. This is the expected temperature range for chilled food products. If however an enzyme system were selected where the relationship between temperature and reaction rate is non-linear, it is possible to make allowances for this within the system.
  • the disadvantage of the indicating system known from the above specification is that the enzyme catalysed reaction produces a change in pH which then requires a second reaction to indicate the pH change which has occurred.
  • a time temperature indicator is provided which is based on by an enzyme catalysed reaction in which the enzyme catalyses reaction of a substrate to produce directly a differently coloured reaction product.
  • the invention provides a time temperature indicator, characterised by a substrate and an enzyme which catalyses reaction of the substrate to produce directly a reaction product of different colour to the substrate.
  • the enzyme catalysed reaction is chosen such that it fulfils one of the following conditions:-
  • the substrate is either colourless or possesses a pale colour, whilst the product of the reaction is strongly coloured.
  • the substrate is strongly coloured, and the reaction product is pale or colourless.
  • p-nitrophenol is normally colourless, but has a strong yellow colour under alkaline conditions.
  • the enzyme is conveniently provided in association with the substrate at a range of concentrations, such that the rate of colour change varies only with time given a constant temperature.
  • the principal advantage of the invention over the known indicator system, which uses lipase to degrade a lipid and then measures the resulting.pH change, is that it gives a direct reading, not affected by the variability and relative insensitivity of pH measurement.
  • Another advantage inherent in the invention is that, provided a suitable enzyme and substrate system is selected, it is possible to have a fully food compatible system such that all reagents are natural food products and no food contamination problems exist.
  • Still another advantage is that, given a suitable carrier system for the enzyme and substrate systems, they may be directly incorporated into a package and are therefore not removable for tampering purposes. They may, however, be produced in an adhesive label format if required.
  • non-impact printing technology specifically ink jet printing
  • ink jet printing to lay down the enzyme component in a label
  • the label may have any format that is required, due to the flexibilty of the production process.
  • the following physical formats of the label may be produced:
  • a self adhesive paper label where the paper is impregnated with the enzyme substrate, and then the enzyme solution applied to the label using one of a variety of printing techniques to give a single layer label with the required graphics.
  • the finished labels may then be stored frozen until applied to a food package.
  • a paper label containing the enzyme substrate may be laminated on to the product package, during the production process, and then overprinted with the enzyme solution prior to the package entering a chill tunnel.
  • the enzyme substrate may be directly incorporated into an ink formulation, such that it constitutes a label directly printed on to the packaging material.
  • the enzyme solution may then be overprinted on to the substrate patch at a convenient later time, using a second ink formulation.
  • This format has the specific advantage that it is integral"with the packaging material, and so cannot be removed from the food package, possibly to be tampered with. It is desirable to ensure that the labelling sets in a permeable and water proof fashion on curing.
  • the graphics format of the label is also extremely adaptable because of the use of ink jet printing.
  • Preferred formats are:
  • a blush label Using grey scale printing technology and a carefully selected substrate which allows limited wicking, it is possible to produce a label such that colour change runs up the label with time. The colour therefore appears to "blush” up the label. Thus, the distance the colour front " has advanced up the label can be used to quantify how much of the total time/temperature history has been used.
  • Another advantage of this format is that it can be utilised to run into the bar code on the package, thus interfering with scanning equipment, so that out of date articles will not pass through a check out scanner.
  • a clock format This is a circular format where colour change develops like the sweeping action of a clock hand. This format can if desired be quantified to show the percentage of shelf life remaining.
  • the invention provides a method of time temperature indication, characterised by use of an enzyme substrate to produce directly a reaction product of different colour to the substrate, and monitoring change of colour of the substrate with time.
  • the illustrated embodiments each comprise a label impregnated with p-nitrophenol and carrying a layer of alkaline phosphatase laid down, eg by ink jet printing, in a pattern of varying concentration.
  • Figures 1 to 3 show three examples of blush label, with enzyme concentration increasingly progressively from left to right.
  • colouring progresses from left to right to indicate the days of shelf life remaining, assuming the product is stored at 4°C.
  • the label of Figure 2 is used to indicate acceptance criteria for the product, at the stages of manufacture, distribution and offer for sale.
  • Figure 3 shows a label marked to show the percentage of remaining shelf life, again as colouring progresses from left to right in accordance with time/temperature conditions of storage.
  • a printed message appears at the end of shelf life, whilst in the label of Figure 5 a coloured window appears at the end of shelf-life.
  • Figure 6 shows a label having a clock format, wherein enzyme concentration increases progressively around the clock and colouring progresses around the clock to indicate usable product life as a percentage of its full life.
  • Colouring may commence, for example, approximately in the five o'clock position, for example, so that in the illustration, when this colouring has extended clockwise to beyond the seven o'clock position, nearly 25% of the product life has been used up, depending on the time for which the product has been stored since manufacture and the temperature conditions which have prevailed during that time period.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Temperature Or Quantity Of Heat (AREA)

Abstract

Indicateur de température-temps, servant à indiquer notamment la durée de vie d'un produit, dans lequel une étiquette, directement incorporée dans ou appliquée à un emballage, porte un substrat qui est catalysé par une enzyme appliquée en un concentration progressive pour obtenir directement un produit de réaction de couleurs différentes, et dont le développement progresse le long ou autour de l'étiquette en fonction des conditions de temps et de température.
PCT/GB1991/001538 1990-09-18 1991-09-10 Indicateur de temperature-temps WO1992005415A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB909020364A GB9020364D0 (en) 1990-09-18 1990-09-18 Time temperature indicator
GB9020364.7 1990-09-18

Publications (1)

Publication Number Publication Date
WO1992005415A1 true WO1992005415A1 (fr) 1992-04-02

Family

ID=10682381

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1991/001538 WO1992005415A1 (fr) 1990-09-18 1991-09-10 Indicateur de temperature-temps

Country Status (2)

Country Link
GB (1) GB9020364D0 (fr)
WO (1) WO1992005415A1 (fr)

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2714474A1 (fr) * 1993-12-23 1995-06-30 Marconi Gec Ltd Procédé, composition et combinaison de marquage enzymatique, et trousse de test et substance marquée correspondantes.
WO2001025472A1 (fr) * 1999-10-05 2001-04-12 Bioett Ab Biocapteur et son utilisation indiquant l'etat d'un produit
WO2006015961A3 (fr) * 2004-08-11 2006-06-01 Ciba Sc Holding Ag Indicateur de température dans le temps à base d’enzyme
GB2435632A (en) * 2006-03-03 2007-09-05 Canon Europa Nv Manufacturing an environmentally sensitive label
US7287001B1 (en) * 1995-07-31 2007-10-23 Ipms, Inc. Electronic product information display system
US7294379B2 (en) 2002-03-07 2007-11-13 Avery Dennison Corporation Color changing device for time indicating label and methods of making and using the same
DE102007056915A1 (de) * 2007-11-27 2009-05-28 Bizerba Gmbh & Co. Kg Drucker und Verfahren zur Warenkennzeichnung
WO2009040547A3 (fr) * 2007-09-26 2009-09-11 Intray Limited Dispositif d'indicateur de temps
EP2333505A1 (fr) * 2009-12-08 2011-06-15 Basf Se Composition et procédé de surveillance de la température de produits réfrigérés et congelés
CN102175677A (zh) * 2011-01-20 2011-09-07 江南大学 基于脂肪酶反应扩散的时间温度指示器的制备方法和应用
CN102495052A (zh) * 2011-12-02 2012-06-13 江南大学 糖化酶型时间-温度指示器
WO2013050846A2 (fr) 2011-10-04 2013-04-11 Sanko Tekstil Isletmeleri Sanayi Ve Ticaret A.S. Étiquette intelligente indiquant une température et procédé associé
US9367851B2 (en) 2009-09-17 2016-06-14 Information Planning & Management Service, Inc. System and method for managing compliance with retail display regulations across a plurality of jurisdictions
WO2017103206A1 (fr) * 2015-12-17 2017-06-22 Keep-It Technologies As Système indicateur de temps-température imprimable

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0132830A2 (fr) * 1983-07-25 1985-02-13 EASTMAN KODAK COMPANY (a New Jersey corporation) Elément analytique sec pour l'essai de phosphatase alcaline
EP0310014A2 (fr) * 1987-09-30 1989-04-05 BEHRINGWERKE Aktiengesellschaft Substrat chromogène
US4826762A (en) * 1987-10-23 1989-05-02 Massachusetts Industry Of Technology Enzymatic temperature change indicator

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0132830A2 (fr) * 1983-07-25 1985-02-13 EASTMAN KODAK COMPANY (a New Jersey corporation) Elément analytique sec pour l'essai de phosphatase alcaline
EP0310014A2 (fr) * 1987-09-30 1989-04-05 BEHRINGWERKE Aktiengesellschaft Substrat chromogène
US4826762A (en) * 1987-10-23 1989-05-02 Massachusetts Industry Of Technology Enzymatic temperature change indicator

Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2714474A1 (fr) * 1993-12-23 1995-06-30 Marconi Gec Ltd Procédé, composition et combinaison de marquage enzymatique, et trousse de test et substance marquée correspondantes.
US8910864B2 (en) 1995-07-31 2014-12-16 Information Planning & Management Service, Inc. Electronic product information display system
US7287001B1 (en) * 1995-07-31 2007-10-23 Ipms, Inc. Electronic product information display system
RU2266333C2 (ru) * 1999-10-05 2005-12-20 Биоэтт Аб Биологический сенсор для индикации изменения состояния продукта, метка и способ индикации изменения состояния продуктов
WO2001025472A1 (fr) * 1999-10-05 2001-04-12 Bioett Ab Biocapteur et son utilisation indiquant l'etat d'un produit
US6642016B1 (en) 1999-10-05 2003-11-04 Bioett Ab Biosensor and its use to indicate the status of a product
US7294379B2 (en) 2002-03-07 2007-11-13 Avery Dennison Corporation Color changing device for time indicating label and methods of making and using the same
US7736866B2 (en) 2004-08-11 2010-06-15 Ciba Corporation Enzyme-based time temperature indicator
WO2006015961A3 (fr) * 2004-08-11 2006-06-01 Ciba Sc Holding Ag Indicateur de température dans le temps à base d’enzyme
GB2435632A (en) * 2006-03-03 2007-09-05 Canon Europa Nv Manufacturing an environmentally sensitive label
WO2009040547A3 (fr) * 2007-09-26 2009-09-11 Intray Limited Dispositif d'indicateur de temps
US8104949B2 (en) 2007-09-26 2012-01-31 Intray Limited Time indicator device
DE102007056915A1 (de) * 2007-11-27 2009-05-28 Bizerba Gmbh & Co. Kg Drucker und Verfahren zur Warenkennzeichnung
US9367851B2 (en) 2009-09-17 2016-06-14 Information Planning & Management Service, Inc. System and method for managing compliance with retail display regulations across a plurality of jurisdictions
US11715115B2 (en) 2009-09-17 2023-08-01 Information Planning & Management Service Inc. System and method for managing compliance with retail display regulations across a plurality of jurisdictions
US10699279B2 (en) 2009-09-17 2020-06-30 Information Planning And Management Service Inc. System and method for managing compliance with retail display regulations across a plurality of jurisdictions
EP2333505A1 (fr) * 2009-12-08 2011-06-15 Basf Se Composition et procédé de surveillance de la température de produits réfrigérés et congelés
WO2011070491A1 (fr) * 2009-12-08 2011-06-16 Basf Se Composition et procédé permettant de contrôler la température de produits réfrigérés et congelés
CN102175677A (zh) * 2011-01-20 2011-09-07 江南大学 基于脂肪酶反应扩散的时间温度指示器的制备方法和应用
US8695528B2 (en) 2011-10-04 2014-04-15 Sanko Tekstil Isletmeleri Sanayi Ve Ticaret A.S. Intelligent temperature indicator label and method
WO2013050846A2 (fr) 2011-10-04 2013-04-11 Sanko Tekstil Isletmeleri Sanayi Ve Ticaret A.S. Étiquette intelligente indiquant une température et procédé associé
CN102495052A (zh) * 2011-12-02 2012-06-13 江南大学 糖化酶型时间-温度指示器
WO2017103206A1 (fr) * 2015-12-17 2017-06-22 Keep-It Technologies As Système indicateur de temps-température imprimable
US10859546B2 (en) 2015-12-17 2020-12-08 Keep-It Technologies As Printable time-temperature indicator system

Also Published As

Publication number Publication date
GB9020364D0 (en) 1990-10-31

Similar Documents

Publication Publication Date Title
WO1992005415A1 (fr) Indicateur de temperature-temps
EP2288879B1 (fr) Système de surveillance fondé sur l'attaque de métaux
RU2507516C2 (ru) Мониторная система, основанная на травлении металлов
US3768976A (en) Temperature-time integrating indicator
US5602804A (en) Long term rapid color changing time indicator
EP1781805B1 (fr) Indicatrice du temps et temperature enzymatique
EP2674296B1 (fr) Matériau d'emballage stratié comprenant un matériau sensible au laser et un matériau aimantable
EP1901925B1 (fr) Etiquette avec une fonction de controle de la temperature, emballage pour produit comportant l`etiquette, et methode pour l` application des etiquettes aux emballages de produits
US8228201B2 (en) Placard having embedded RFID device for tracking objects
US5228573A (en) Pharmaceutical capsule and method of making
WO1999030148A1 (fr) Indicateur de duree de conservation
JP2011530085A6 (ja) 金属のエッチングに基づくモニタリングシステム
KR101062814B1 (ko) 패키지 내의 변화를 인지하기 위한 방법, 패키지의 위치를 파악하기 위한 방법, 패키지 및 패키지의 위치를 파악하기 위한 시스템
EP0772802A1 (fr) Indicateur de temps comprenant un adhesif discontinu
CN101894498A (zh) 时间温度累加指示标签
US3977945A (en) Time and temperature-dependent enzymatic indicator
US7808861B2 (en) Expiration date system
WO2002017281A1 (fr) Etiquette de date pour un emballage
EP1038288A1 (fr) Etiquette
WO2006058955A1 (fr) Procédé pour équiper des paquets avec des indicateurs et système d’indicateurs
JP2017057245A (ja) 時間経過インジケータ
FI62026C (fi) Foerfarande och anordningar i en pappersfabrik foer identifiering av pappersrullar
CN113861861B (zh) 一种双面胶带及其加工方法
RU2045U1 (ru) Метка для маркировки продуктов питания и товаров народного потребления
GB2390592A (en) Condition sensitive label

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): CA FI GB JP NO US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU NL SE

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载