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WO1992004469A3 - Procede de detection d'acide nucleique utilisant l'agglutination de particules - Google Patents

Procede de detection d'acide nucleique utilisant l'agglutination de particules Download PDF

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Publication number
WO1992004469A3
WO1992004469A3 PCT/GB1991/001517 GB9101517W WO9204469A3 WO 1992004469 A3 WO1992004469 A3 WO 1992004469A3 GB 9101517 W GB9101517 W GB 9101517W WO 9204469 A3 WO9204469 A3 WO 9204469A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
particle reagent
detection method
agglutination
acid detection
Prior art date
Application number
PCT/GB1991/001517
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English (en)
Other versions
WO1992004469A2 (fr
Inventor
Allen Frank Giles
Karie Joanne Booth
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Ici Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Ici Plc filed Critical Ici Plc
Publication of WO1992004469A2 publication Critical patent/WO1992004469A2/fr
Publication of WO1992004469A3 publication Critical patent/WO1992004469A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé servant à détecter la présence de séquences d'acide nucléique par agglutination d'un réactif à particules génériques. Les procédés connus nécessitent un réactif à particules spécifiques pour chaque séquence devant être détectée. En présence d'une séquence d'acide nucléique donnée, une espèce d'acide nucléique, comprenant au moins deux partenaires de liaison, est produite. Le réactif à particules génériques est recouvert par un partenaire de liaison spécifique qui peut se lier aux partenaires de liaison de l'espèce d'acide nucléique et permet ainsi l'agglutination de la particule. Le procédé peut aussi être utilisé pour détecter des mutations dans une séquence d'acide nucléique. Un mode préféré de réalisation utilise un haptène tel que de la fluorescéine comme partenaire de liaison dans l'espèce d'acide nucléique et des particules recouvertes d'un anticorps anti-haptène tel que du latex recouvert d'un anticorps anti-fluorescéine comme le reáctif à particules génériques.
PCT/GB1991/001517 1990-09-06 1991-09-06 Procede de detection d'acide nucleique utilisant l'agglutination de particules WO1992004469A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9019512.4 1990-09-06
GB909019512A GB9019512D0 (en) 1990-09-06 1990-09-06 Assay method

Publications (2)

Publication Number Publication Date
WO1992004469A2 WO1992004469A2 (fr) 1992-03-19
WO1992004469A3 true WO1992004469A3 (fr) 1992-05-14

Family

ID=10681798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1991/001517 WO1992004469A2 (fr) 1990-09-06 1991-09-06 Procede de detection d'acide nucleique utilisant l'agglutination de particules

Country Status (3)

Country Link
AU (1) AU8505091A (fr)
GB (1) GB9019512D0 (fr)
WO (1) WO1992004469A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles

Families Citing this family (34)

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Publication number Priority date Publication date Assignee Title
US5601978A (en) * 1993-09-03 1997-02-11 Abbott Laboratories Oligonucleotides and methods for the detection of chlamydia trachomatis
US6060288A (en) * 1994-08-03 2000-05-09 Mosaic Technologies Method for performing amplification of nucleic acid on supports
US6090592A (en) * 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5747256A (en) * 1995-12-19 1998-05-05 Beckman Instruments, Inc. Homogeneous DNA probe titration assay
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
ATE545710T1 (de) 1997-04-01 2012-03-15 Illumina Cambridge Ltd Verfahren zur vervielfältigung von nukleinsäuren
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
JP2002509443A (ja) * 1997-10-31 2002-03-26 ジェン−プローブ・インコーポレーテッド 核酸検出法
US6080544A (en) * 1998-02-13 2000-06-27 Ohio State University Methods for identifying nucleic acid mutations using mismatch modification
EP1198591B1 (fr) * 1999-06-25 2011-03-16 Nanosphere, Inc. Nanoparticules auxquelles sont rattaches des oligonucleotides et leurs utilisations
AU774593C (en) 2000-01-13 2005-06-23 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6602669B2 (en) 2000-07-11 2003-08-05 Northwestern University Method of detection by enhancement of silver staining
DE10196758T1 (de) * 2000-10-05 2003-09-04 Palti Yoram Prof Geometrisch wirksame Teilchen-Agglutination insbesondere zum Nachweis einer geringen Affinitätsbindung
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
DE60228128D1 (de) 2001-11-09 2008-09-18 Nanosphere Inc Biokonjugat-nanopartikelsonden
ATE420357T1 (de) 2002-07-02 2009-01-15 Nanosphere Inc Nanopartikel-polyanion-konjugate sowie verfahren zur verwendung davon beim nachweis von analyten
US8372648B2 (en) 2003-04-16 2013-02-12 APDN (B.V.I.), Inc. Optical reporter compositions
US8124333B2 (en) * 2003-04-16 2012-02-28 APDN, Inc. Methods for covalent linking of optical reporters
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
WO2008093098A2 (fr) 2007-02-02 2008-08-07 Illumina Cambridge Limited Procedes pour indexer des echantillons et sequencer de multiples matrices nucleotidiques
WO2010038042A1 (fr) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Enrichissement d'échantillons d'acide nucléique pour des applications de séquençage
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
US9904734B2 (en) 2013-10-07 2018-02-27 Apdn (B.V.I.) Inc. Multimode image and spectral reader
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
CN106103121B (zh) 2014-03-18 2019-12-06 亚普蒂恩(B.V.I.)公司 用于安全应用的加密光学标记物
EP3442719B1 (fr) 2016-04-11 2021-09-01 APDN (B.V.I.) Inc. Procédé de marquage de produits cellulosiques
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
WO2018156352A1 (fr) 2017-02-21 2018-08-30 Apdn (B.V.I) Inc. Particules submicroniques enrobées d'acide nucléique pour une authentification

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987005334A1 (fr) * 1986-03-04 1987-09-11 Angenics, Inc. Detection d'acides nucleiques par la determination de l'agglutination de particules
WO1990002205A1 (fr) * 1988-08-25 1990-03-08 Angenics, Inc. Detection de sequences d'acides nucleiques par agglutination de particules
EP0332435B1 (fr) * 1988-03-10 1992-04-22 Zeneca Limited Procédé pour détecter des séquences de nucléotides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987005334A1 (fr) * 1986-03-04 1987-09-11 Angenics, Inc. Detection d'acides nucleiques par la determination de l'agglutination de particules
EP0332435B1 (fr) * 1988-03-10 1992-04-22 Zeneca Limited Procédé pour détecter des séquences de nucléotides
WO1990002205A1 (fr) * 1988-08-25 1990-03-08 Angenics, Inc. Detection de sequences d'acides nucleiques par agglutination de particules

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7238472B2 (en) 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles

Also Published As

Publication number Publication date
AU8505091A (en) 1992-03-30
GB9019512D0 (en) 1990-10-24
WO1992004469A2 (fr) 1992-03-19

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