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WO1992004469A3 - Procede de detection d'acide nucleique utilisant l'agglutination de particules - Google Patents

Procede de detection d'acide nucleique utilisant l'agglutination de particules Download PDF

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Publication number
WO1992004469A3
WO1992004469A3 PCT/GB1991/001517 GB9101517W WO9204469A3 WO 1992004469 A3 WO1992004469 A3 WO 1992004469A3 GB 9101517 W GB9101517 W GB 9101517W WO 9204469 A3 WO9204469 A3 WO 9204469A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
particle reagent
detection method
agglutination
acid detection
Prior art date
Application number
PCT/GB1991/001517
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English (en)
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WO1992004469A2 (fr
Inventor
Allen Frank Giles
Karie Joanne Booth
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Ici Plc
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Publication date
Application filed by Ici Plc filed Critical Ici Plc
Publication of WO1992004469A2 publication Critical patent/WO1992004469A2/fr
Publication of WO1992004469A3 publication Critical patent/WO1992004469A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé servant à détecter la présence de séquences d'acide nucléique par agglutination d'un réactif à particules génériques. Les procédés connus nécessitent un réactif à particules spécifiques pour chaque séquence devant être détectée. En présence d'une séquence d'acide nucléique donnée, une espèce d'acide nucléique, comprenant au moins deux partenaires de liaison, est produite. Le réactif à particules génériques est recouvert par un partenaire de liaison spécifique qui peut se lier aux partenaires de liaison de l'espèce d'acide nucléique et permet ainsi l'agglutination de la particule. Le procédé peut aussi être utilisé pour détecter des mutations dans une séquence d'acide nucléique. Un mode préféré de réalisation utilise un haptène tel que de la fluorescéine comme partenaire de liaison dans l'espèce d'acide nucléique et des particules recouvertes d'un anticorps anti-haptène tel que du latex recouvert d'un anticorps anti-fluorescéine comme le reáctif à particules génériques.
PCT/GB1991/001517 1990-09-06 1991-09-06 Procede de detection d'acide nucleique utilisant l'agglutination de particules WO1992004469A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB909019512A GB9019512D0 (en) 1990-09-06 1990-09-06 Assay method
GB9019512.4 1990-09-06

Publications (2)

Publication Number Publication Date
WO1992004469A2 WO1992004469A2 (fr) 1992-03-19
WO1992004469A3 true WO1992004469A3 (fr) 1992-05-14

Family

ID=10681798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1991/001517 WO1992004469A2 (fr) 1990-09-06 1991-09-06 Procede de detection d'acide nucleique utilisant l'agglutination de particules

Country Status (3)

Country Link
AU (1) AU8505091A (fr)
GB (1) GB9019512D0 (fr)
WO (1) WO1992004469A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles

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US5601978A (en) * 1993-09-03 1997-02-11 Abbott Laboratories Oligonucleotides and methods for the detection of chlamydia trachomatis
US6060288A (en) * 1994-08-03 2000-05-09 Mosaic Technologies Method for performing amplification of nucleic acid on supports
US6090592A (en) * 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5747256A (en) * 1995-12-19 1998-05-05 Beckman Instruments, Inc. Homogeneous DNA probe titration assay
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
EP2327797B1 (fr) 1997-04-01 2015-11-25 Illumina Cambridge Limited Methode de séquençage d'acide nucléique
US6974669B2 (en) 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
WO1999023258A1 (fr) * 1997-10-31 1999-05-14 Gen-Probe Incorporated Procedes de detection d'acide nucleique
US6080544A (en) * 1998-02-13 2000-06-27 Ohio State University Methods for identifying nucleic acid mutations using mismatch modification
ATE502117T1 (de) * 1999-06-25 2011-04-15 Nanosphere Inc Nanopartikel mit gebundenen oligonukleotiden und deren verwendungen
WO2001051665A2 (fr) 2000-01-13 2001-07-19 Nanosphere Inc. Nanoparticules auxquelles sont attaches des oligonucleotides et utilisations de ces dernieres
ATE324465T1 (de) 2000-07-11 2006-05-15 Univ Northwestern Nachweismethode mittels verstärkter silberfärbung
US20030175994A1 (en) * 2000-10-05 2003-09-18 Yoram Palti Geometrically efficient particle agglutination, particularly to detect low affinity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
JP4220397B2 (ja) 2001-11-09 2009-02-04 ナノスフェアー インコーポレイテッド バイオコンジュゲート‐ナノ粒子プローブ
US7253277B2 (en) 2002-07-02 2007-08-07 Nanosphere, Inc. Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
US8372648B2 (en) 2003-04-16 2013-02-12 APDN (B.V.I.), Inc. Optical reporter compositions
US8124333B2 (en) * 2003-04-16 2012-02-28 APDN, Inc. Methods for covalent linking of optical reporters
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
WO2008093098A2 (fr) 2007-02-02 2008-08-07 Illumina Cambridge Limited Procedes pour indexer des echantillons et sequencer de multiples matrices nucleotidiques
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
US9904734B2 (en) 2013-10-07 2018-02-27 Apdn (B.V.I.) Inc. Multimode image and spectral reader
ES2978925T3 (es) 2014-03-18 2024-09-23 Apdn Bvi Inc Marcadores ópticos cifrados para aplicaciones de seguridad
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
WO2017180302A1 (fr) 2016-04-11 2017-10-19 Apdn (B.V.I.) Inc. Procédé de marquage de produits cellulosiques
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
US10920274B2 (en) 2017-02-21 2021-02-16 Apdn (B.V.I.) Inc. Nucleic acid coated submicron particles for authentication

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987005334A1 (fr) * 1986-03-04 1987-09-11 Angenics, Inc. Detection d'acides nucleiques par la determination de l'agglutination de particules
WO1990002205A1 (fr) * 1988-08-25 1990-03-08 Angenics, Inc. Detection de sequences d'acides nucleiques par agglutination de particules
EP0332435B1 (fr) * 1988-03-10 1992-04-22 Zeneca Limited Procédé pour détecter des séquences de nucléotides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987005334A1 (fr) * 1986-03-04 1987-09-11 Angenics, Inc. Detection d'acides nucleiques par la determination de l'agglutination de particules
EP0332435B1 (fr) * 1988-03-10 1992-04-22 Zeneca Limited Procédé pour détecter des séquences de nucléotides
WO1990002205A1 (fr) * 1988-08-25 1990-03-08 Angenics, Inc. Detection de sequences d'acides nucleiques par agglutination de particules

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7115688B1 (en) 1998-11-30 2006-10-03 Nanosphere, Inc. Nanoparticles with polymer shells
US7135055B2 (en) 2001-05-25 2006-11-14 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7147687B2 (en) 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7238472B2 (en) 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles

Also Published As

Publication number Publication date
AU8505091A (en) 1992-03-30
GB9019512D0 (en) 1990-10-24
WO1992004469A2 (fr) 1992-03-19

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