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WO1992003559A3 - Test system for checking the activity of viral proteinases - Google Patents

Test system for checking the activity of viral proteinases Download PDF

Info

Publication number
WO1992003559A3
WO1992003559A3 PCT/EP1991/001595 EP9101595W WO9203559A3 WO 1992003559 A3 WO1992003559 A3 WO 1992003559A3 EP 9101595 W EP9101595 W EP 9101595W WO 9203559 A3 WO9203559 A3 WO 9203559A3
Authority
WO
WIPO (PCT)
Prior art keywords
checking
activity
test system
viral proteinases
viral
Prior art date
Application number
PCT/EP1991/001595
Other languages
German (de)
French (fr)
Other versions
WO1992003559A2 (en
Inventor
Wolfgang Sommergruber
Hans-Dieter Liebig
Timothy Skern
Marion Luderer
Dieter Blass
Ernst Kuechler
Heinrich Kowalski
Ljerka Frasel
Herbert Auer
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19904027153 external-priority patent/DE4027153A1/en
Priority claimed from DE19914112952 external-priority patent/DE4112952A1/en
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Priority to JP91513892A priority Critical patent/JPH05506999A/en
Publication of WO1992003559A2 publication Critical patent/WO1992003559A2/en
Publication of WO1992003559A3 publication Critical patent/WO1992003559A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22029—Picornain 2A (3.4.22.29)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention concerns an in vivo colour test system for checking the activity of viral proteases.
PCT/EP1991/001595 1990-08-28 1991-08-22 Test system for checking the activity of viral proteinases WO1992003559A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP91513892A JPH05506999A (en) 1990-08-28 1991-08-22 Test system for monitoring viral proteinase activity

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19904027153 DE4027153A1 (en) 1990-08-28 1990-08-28 New DNA encoding fusion protein contg. M13 alpha fragment
DEP4027153.6 1990-08-28
DEP4112952.0 1991-04-20
DE19914112952 DE4112952A1 (en) 1991-04-20 1991-04-20 New DNA encoding fusion protein contg. M13 alpha fragment

Publications (2)

Publication Number Publication Date
WO1992003559A2 WO1992003559A2 (en) 1992-03-05
WO1992003559A3 true WO1992003559A3 (en) 1992-04-30

Family

ID=25896304

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1991/001595 WO1992003559A2 (en) 1990-08-28 1991-08-22 Test system for checking the activity of viral proteinases

Country Status (4)

Country Link
EP (1) EP0497949A1 (en)
JP (1) JPH05506999A (en)
CA (1) CA2072154A1 (en)
WO (1) WO1992003559A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX9206981A (en) * 1991-12-06 1994-08-31 Whitehead Biomedical Inst RECOMBINANT VACCINES AND METHOD FOR PRODUCING THEM.
US5965124A (en) * 1991-12-06 1999-10-12 Whitehead Institute For Biomedical Research Replication-competent recombinant viral vaccines and method of producing same
US5763196A (en) * 1996-01-26 1998-06-09 Boehringer Mannheim Corporation Assays using cross-linked polypeptide fragments of β-galactosidase
ATE211480T1 (en) * 1996-01-26 2002-01-15 Roche Diagnostics Corp BISMALEIC ACID IMIDE CROSSLINKER
US5976857A (en) * 1996-01-26 1999-11-02 Boehringer Mannheim Corporation Cross-linked polypeptide fragments of β-galactosidase
WO1999006072A1 (en) * 1997-07-30 1999-02-11 Boehringer Mannheim Corporation Cyclized prodrugs
AU2217800A (en) * 1998-12-24 2000-07-31 Small Molecule Therapeutics, Inc. Methods and compositions for identifying protease modulators
AU2007225195B2 (en) 2006-03-13 2013-10-24 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions using a reduced affinity enzyme complementation reporter system
US8101373B2 (en) 2007-10-12 2012-01-24 Discoverx Corporation β-galactosidase donor fragments
WO2012178079A1 (en) 2011-06-23 2012-12-27 Discoverx Corporation Monitoring protein trafficking using beta-galactosidase reporter fragment complementation

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2712615A1 (en) * 1977-03-18 1978-09-21 Max Planck Gesellschaft PROCESS FOR MANUFACTURING FILAMENTOES PHAGEN AS A VECTOR FOR SYNTHETIC RECOMBINATES
EP0321973A2 (en) * 1987-12-23 1989-06-28 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Expression of the virally encoded protease P2A of HRV2
DE3819846A1 (en) * 1988-06-10 1989-12-14 Hans Prof Dr Dr Wolf Test system for developing HIV protease inhibitors
WO1990001061A1 (en) * 1988-07-25 1990-02-08 Boehringer Ingelheim International Gmbh In vitro synthesis of an infectious rna

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2712615A1 (en) * 1977-03-18 1978-09-21 Max Planck Gesellschaft PROCESS FOR MANUFACTURING FILAMENTOES PHAGEN AS A VECTOR FOR SYNTHETIC RECOMBINATES
EP0321973A2 (en) * 1987-12-23 1989-06-28 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Expression of the virally encoded protease P2A of HRV2
DE3819846A1 (en) * 1988-06-10 1989-12-14 Hans Prof Dr Dr Wolf Test system for developing HIV protease inhibitors
WO1990001061A1 (en) * 1988-07-25 1990-02-08 Boehringer Ingelheim International Gmbh In vitro synthesis of an infectious rna

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Gene, Band 60, 1987, Amsterdam (NL), S. Jia et al.: "Carbohydrate-binding protein 35: molecular cloning and expression of a recombinant polypeptide with lectin activity", Seiten 197-204, siehe Zusammenfassung; Seite 201, unter d) *
J. Sambrook et al.: "Molecular cloning", 2. Ausgabe,1989, Cold Spring Harbor Laboratory Press (US), Seiten 18.81 - 18.88, siehe Seite 18.81, 2. Absatz *
Proc. Natl. Acad. Sci., Band 88, Juli 1991, Washington (US), H.-D. Liebig et al.: "Proteinase trapping: Screening for viral proteinase mutants by alpha-complementation" Seiten 5979-5983, siehe das ganze Dokument *
Science, Band 206, 19. Oktober 1979, Lancaster (US), A. Van Ooyen et al.: "Comparison of total sequence of a cloned rabbit beta-globin ene and its flanking regions with a homologous mouse sequence", Seiten 337-344, siehe Abbildung 3, Position 1 (In der Anmeldung erwähnt) *

Also Published As

Publication number Publication date
JPH05506999A (en) 1993-10-14
EP0497949A1 (en) 1992-08-12
WO1992003559A2 (en) 1992-03-05
CA2072154A1 (en) 1992-03-01

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