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WO1992002815A3 - Surveillance et analyse quantitative de la structure des proteines par chromatographie soustractive - Google Patents

Surveillance et analyse quantitative de la structure des proteines par chromatographie soustractive Download PDF

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Publication number
WO1992002815A3
WO1992002815A3 PCT/US1991/005544 US9105544W WO9202815A3 WO 1992002815 A3 WO1992002815 A3 WO 1992002815A3 US 9105544 W US9105544 W US 9105544W WO 9202815 A3 WO9202815 A3 WO 9202815A3
Authority
WO
WIPO (PCT)
Prior art keywords
concentration
monitoring
sample
quantitative analysis
solution
Prior art date
Application number
PCT/US1991/005544
Other languages
English (en)
Other versions
WO1992002815A2 (fr
Inventor
Noubar B Afeyan
Fred E Regnier
Original Assignee
Perseptive Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perseptive Biosystems Inc filed Critical Perseptive Biosystems Inc
Priority to CA002088360A priority Critical patent/CA2088360A1/fr
Priority to AU85306/91A priority patent/AU654503B2/en
Publication of WO1992002815A2 publication Critical patent/WO1992002815A2/fr
Publication of WO1992002815A3 publication Critical patent/WO1992002815A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N2030/626—Detectors specially adapted therefor calibration, baseline
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On décrit des procédés destinés à effectuer des évaluations en temps réel de la concentration d'un soluté cible ainsi qu'à contrôler le profil structurel de ce soluté cible dans une solution. Des techniques chromatographiques à affinité, à liaison non difusante, sont utilisées pour produire des chromatogrammes présentant des courbes de percée nettement définies. En fonction de la différence entre la concentration détectée à l'équilibre des impuretés qui passent à travers la matrice et la concentration de tous les solutés de l'échantillon, la concentration du soluté cible dans la solution peut être déterminée. Alternativement, un chromatogramme représentant le profil structurel d'une protéine dans un échantillon comprenant un mélange de variantes structurelles peut être produit d'après le procédé de l'invention. Les dosages peuvent être effectués en temps réel de sorte que la production ou la purification d'une substance précieuse telle qu'une protéine de recombinaison pharmaceutique ou une substance analogue peut être contrôlée de manière significative.
PCT/US1991/005544 1990-08-10 1991-08-01 Surveillance et analyse quantitative de la structure des proteines par chromatographie soustractive WO1992002815A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002088360A CA2088360A1 (fr) 1990-08-10 1991-08-01 Analyse quantitative et surveillance de la structure d'une proteine par chromatographie par soustraction
AU85306/91A AU654503B2 (en) 1990-08-10 1991-08-01 Quantitative analysis and monitoring of protein structure by subtractive chromatography

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56612190A 1990-08-10 1990-08-10
US566,121 1990-08-10

Publications (2)

Publication Number Publication Date
WO1992002815A2 WO1992002815A2 (fr) 1992-02-20
WO1992002815A3 true WO1992002815A3 (fr) 1992-04-30

Family

ID=24261583

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/005544 WO1992002815A2 (fr) 1990-08-10 1991-08-01 Surveillance et analyse quantitative de la structure des proteines par chromatographie soustractive

Country Status (5)

Country Link
EP (1) EP0548178A1 (fr)
JP (1) JPH06500396A (fr)
AU (1) AU654503B2 (fr)
CA (1) CA2088360A1 (fr)
WO (1) WO1992002815A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE147281T1 (de) * 1991-03-28 1997-01-15 Perseptive Biosystems Inc Produktinformation in chromatographischem effluenten durch differenzbildung
CA2240325A1 (fr) 1998-03-27 1998-11-14 Synsorb Biotech, Inc. Methodes de criblage de banques de constituants
US6054047A (en) * 1998-03-27 2000-04-25 Synsorb Biotech, Inc. Apparatus for screening compound libraries
US6720190B1 (en) 1998-03-27 2004-04-13 Ole Hindsgaul Methods for screening compound libraries
US6613575B1 (en) 1998-03-27 2003-09-02 Ole Hindsgaul Methods for screening compound libraries
DE19945351C2 (de) * 1999-09-22 2002-04-18 Lohmann Therapie Syst Lts Verfahren zum Auffinden und zur Isolierung pharmakologisch wirksamer Verbindungen aus Substanzgemischen
EP2446257B1 (fr) * 2009-06-26 2020-06-03 GE Healthcare Bio-Sciences AB Procédé dans un système de chromatographie
CA3123030C (fr) * 2018-10-09 2023-04-04 C Technologies, Inc. Systeme de commande de masse pour chromatographie
CN114280208B (zh) * 2021-11-03 2024-05-24 鼎康(武汉)生物医药有限公司 一种亲和层析填料动态载量的测定方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4204424A (en) * 1979-01-08 1980-05-27 Phillips Petroleum Company Chromatographic analyzer detector gain adjustment
EP0141259A1 (fr) * 1981-03-09 1985-05-15 Environmental Sciences Associates, Inc. Améliorations dans la chromatograhie liquide
WO1990006516A1 (fr) * 1988-12-05 1990-06-14 Primus Corporation Procede de determination d'especes proteiques glucosees

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56114761A (en) * 1980-02-14 1981-09-09 Shinotesuto Kenkyusho:Kk Fractional determination method of zinc in fresh blood serum by affinity chromatography
JPS58216954A (ja) * 1982-06-11 1983-12-16 Shimadzu Corp 低温高速液体クロマトグラフイ
JPS5984828A (ja) * 1982-11-08 1984-05-16 Suntory Ltd 安定化免疫複合体およびその利用方法
IT1150598B (it) * 1982-12-14 1986-12-17 Ines Bianchi Procedimento per la purificazione della superossidodismutasi mediante cromatografia di affinita'
JPS59143958A (ja) * 1983-02-07 1984-08-17 Sekisui Chem Co Ltd フイブロネクチンの定量法
JPS63236964A (ja) * 1987-03-26 1988-10-03 Ube Ind Ltd 糖尿病の診断法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4204424A (en) * 1979-01-08 1980-05-27 Phillips Petroleum Company Chromatographic analyzer detector gain adjustment
EP0141259A1 (fr) * 1981-03-09 1985-05-15 Environmental Sciences Associates, Inc. Améliorations dans la chromatograhie liquide
WO1990006516A1 (fr) * 1988-12-05 1990-06-14 Primus Corporation Procede de determination d'especes proteiques glucosees

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BIOTECHNOLOGY vol. 8, no. 3, March 1990, NEW YORK US pages 203 - 206; AFEYAN ET AL.: 'perfusion chromatography an approach to purifying biomolecules' *
JOURNAL OF BIOLOGICAL CHEMISTRY. vol. 259, no. 6, 1984, BALTIMORE US pages 3796 - 3799; YIN CHANG LIU ET AL.: 'quantitative analysis of protein: immobilized dye interaction' *
JOURNAL OF CHROMATOGRAPHY. vol. 512, 20 July 1990, AMSTERDAM NL pages 365 - 376; LLOYD ET AL.: 'preparative high-performance liquid chromatography on a unique high-speed macroporous resign' *

Also Published As

Publication number Publication date
WO1992002815A2 (fr) 1992-02-20
EP0548178A1 (fr) 1993-06-30
JPH06500396A (ja) 1994-01-13
CA2088360A1 (fr) 1992-02-11
AU8530691A (en) 1992-03-02
AU654503B2 (en) 1994-11-10

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