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WO1992001814A3 - Reduction d'amplification non-specifique au cours d'une amplification in vitro d'acide nucleique utilisant des bases d'acide nucleique modifiees - Google Patents

Reduction d'amplification non-specifique au cours d'une amplification in vitro d'acide nucleique utilisant des bases d'acide nucleique modifiees Download PDF

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Publication number
WO1992001814A3
WO1992001814A3 PCT/US1991/005210 US9105210W WO9201814A3 WO 1992001814 A3 WO1992001814 A3 WO 1992001814A3 US 9105210 W US9105210 W US 9105210W WO 9201814 A3 WO9201814 A3 WO 9201814A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
amplification
reduction
specific amplification
vitro
Prior art date
Application number
PCT/US1991/005210
Other languages
English (en)
Other versions
WO1992001814A2 (fr
Inventor
John J Sninsky
David H Gelfand
Shirley Y Kwok
Original Assignee
Cetus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Corp filed Critical Cetus Corp
Priority to US07/960,362 priority Critical patent/US5418149A/en
Priority to DK91916353T priority patent/DK0540693T3/da
Priority to CA002087724A priority patent/CA2087724C/fr
Priority to EP91916353A priority patent/EP0540693B1/fr
Priority to AU85327/91A priority patent/AU665338B2/en
Priority to JP51560491A priority patent/JP3392863B2/ja
Priority to DE69130800T priority patent/DE69130800T2/de
Publication of WO1992001814A2 publication Critical patent/WO1992001814A2/fr
Publication of WO1992001814A3 publication Critical patent/WO1992001814A3/fr
Priority to GR990401066T priority patent/GR3029987T3/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Des procédés améliorés d'amplification d'acides nucléiques peuvent réduire l'amplification non-spécifique et minimiser les effets de contamination d'analyses de réactions d'amplification d'acide nucléique dus à des produits amplifiés provenant des précédentes amplifications. Ces procédés consistent à introduire des bases de nucléotides non conventionnelles dans les produits de réaction d'amplification et à traiter les produits au moyen d'éléments enzymatiques (par exemple des glycosylases) et/ou physiques-chimiques afin que le produit soit incapable d'agir comme une matrice dans des amplifications subséquentes.
PCT/US1991/005210 1990-07-24 1991-07-23 Reduction d'amplification non-specifique au cours d'une amplification in vitro d'acide nucleique utilisant des bases d'acide nucleique modifiees WO1992001814A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
US07/960,362 US5418149A (en) 1990-07-24 1991-07-23 Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
DK91916353T DK0540693T3 (da) 1990-07-24 1991-07-23 Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy
CA002087724A CA2087724C (fr) 1990-07-24 1991-07-23 Reduction de l'amplification non specifique lors de l'amplification de l'acide nucleique in vitro grace a des bases d'acide nucleique modifiees
EP91916353A EP0540693B1 (fr) 1990-07-24 1991-07-23 REDUCTION D'AMPLIFICATION NON-SPECIFIQUE AU COURS D'UNE AMPLIFICATION $i(IN VITRO) D'ACIDE NUCLEIQUE UTILISANT DES BASES D'ACIDE NUCLEIQUE MODIFIEES
AU85327/91A AU665338B2 (en) 1990-07-24 1991-07-23 The reduction of non-specific amplification during (in vitro) nucleic acid amplification using modified nucleic acid bases
JP51560491A JP3392863B2 (ja) 1990-07-24 1991-07-23 修飾核酸塩基を使用するインビトロ核酸増幅における非特異的増幅の低減
DE69130800T DE69130800T2 (de) 1990-07-24 1991-07-23 Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
GR990401066T GR3029987T3 (en) 1990-07-24 1999-04-19 THE REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING -i(IN VITRO) NUCLEIC ACID AMPLIFICATION USING MODIFIED NUCLEIC ACID BASES

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US55751790A 1990-07-24 1990-07-24
US557,517 1990-07-24
US60915790A 1990-11-02 1990-11-02
US609,157 1990-11-02

Publications (2)

Publication Number Publication Date
WO1992001814A2 WO1992001814A2 (fr) 1992-02-06
WO1992001814A3 true WO1992001814A3 (fr) 1992-05-14

Family

ID=27071456

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/005210 WO1992001814A2 (fr) 1990-07-24 1991-07-23 Reduction d'amplification non-specifique au cours d'une amplification in vitro d'acide nucleique utilisant des bases d'acide nucleique modifiees

Country Status (11)

Country Link
US (1) US5418149A (fr)
EP (1) EP0540693B1 (fr)
JP (1) JP3392863B2 (fr)
AT (1) ATE176002T1 (fr)
AU (1) AU665338B2 (fr)
CA (1) CA2087724C (fr)
DE (1) DE69130800T2 (fr)
DK (1) DK0540693T3 (fr)
ES (1) ES2128323T3 (fr)
GR (1) GR3029987T3 (fr)
WO (1) WO1992001814A2 (fr)

Families Citing this family (182)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
US5856088A (en) * 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
WO1994023069A1 (fr) 1993-03-26 1994-10-13 Gen-Probe Incorporated Detection du virus de type 1 de l'immunodeficience humaine
CA2122203C (fr) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination des reactions d'amplification d'acides nucleiques
EP0632134B1 (fr) * 1993-07-01 2001-09-05 F. Hoffmann-La Roche Ag Réactif et procédé pour la transcription inverse à haute température suivi par la réaction en chaíne de la polymérase
EP1245286B1 (fr) * 1993-10-22 2009-11-25 Abbott Laboratories Tube de réaction et méthode d'utilisation de celui-ci minimisant les contaminations
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) * 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
DE4412286A1 (de) * 1994-04-09 1995-10-12 Boehringer Mannheim Gmbh System zur kontaminationsfreien Bearbeitung von Reaktionsabläufen
FR2722799B1 (fr) * 1994-07-21 1996-10-04 Parteurop Procede d'amplification d'acide nucleique a l'aide d'un nucleoside modifie, et detection du produit d'amplification a l'aide d'anticorps
ZA956776B (en) 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
CA2168712A1 (fr) * 1995-02-07 1996-08-08 John William Henderson Sutherland Utilisation d'exonuclease et (ou) de glycosylate comme supplements d'anticorps anti-polymerase pour augmenter la specificite dans les reactions en chaine de la polymerase
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
JPH11506605A (ja) * 1995-06-07 1999-06-15 アボツト・ラボラトリーズ 増幅反応におけるバックグラウンドを減少させるためにプローブをマスキングする方法
DK0843735T4 (da) * 1995-07-11 2007-01-22 Univ College Cork Nat Univ Ie Glycolasemedieret påvisning af kendte nukleotidsekvenser
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
US5837442A (en) 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
DE19545320A1 (de) * 1995-12-05 1997-06-12 Boehringer Mannheim Gmbh Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA
US5763184A (en) 1996-01-30 1998-06-09 Roche Molecular Systems, Inc. Nucleotide sequence variation in the ABO glycosyltransferase gene
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
US6436638B1 (en) 1996-05-09 2002-08-20 Metropolitan Water District Of Southern California Cryptosporidium detection method
WO1997045559A1 (fr) 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection de differences dans des sequences d'acides nucleiques utilisant une combinaison de la detection par ligase et de reactions d'amplification en chaine par polymerase
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
DK0866071T3 (da) * 1997-03-20 2005-01-17 Hoffmann La Roche Modificerede primere
US5958738A (en) * 1997-03-24 1999-09-28 Roche Diagnostics Corporation Procedure for subtractive hybridization and difference analysis
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
DE19736062A1 (de) * 1997-08-20 1999-02-25 Boehringer Mannheim Gmbh Reduktion von Kreuzkontaminationen bei Nukleinsäureamplifikationen
JP3712255B2 (ja) 1997-12-08 2005-11-02 カリフォルニア・インスティチュート・オブ・テクノロジー ポリヌクレオチドおよびポリペプチド配列を生成するための方法
EP1053348B1 (fr) * 1998-02-04 2010-06-02 Life Technologies Corporation Determination d'un genotype d'un produit d'amplification sur des sites alleliques multiples
US6174680B1 (en) 1998-12-30 2001-01-16 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compounds and uses thereof
JP2002504352A (ja) * 1998-02-23 2002-02-12 デイナ−ファーバー キャンサー インスティチュート，インコーポレイテッド ミスマッチ修復グリコシラーゼ反応性部位を同定する方法、化合物およびそれらの使用
JP4196236B2 (ja) * 1998-03-17 2008-12-17 東洋紡績株式会社 核酸増幅用試薬および配列特異的な核酸増幅法
WO1999050447A1 (fr) * 1998-03-27 1999-10-07 Mira Diagnostika Gmbh Procede et compose d'acide nucleique pour la decomposition de molecules d'acide nucleique synthetisees in vitro
CA2266755A1 (fr) * 1998-04-24 1999-10-24 James M. Dunn Methode de sequencage de polymeres d'acides nucleiques
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US7270958B2 (en) * 1998-09-10 2007-09-18 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
CA2357601A1 (fr) * 1998-12-30 2000-07-06 Dana-Farber Cancer Institute, Inc. Reseau de balayage de mutation et methode d'utilisation
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP1038974A1 (fr) * 1999-03-26 2000-09-27 Mira Diagnostica GmbH Procédé, oligonucleotides pour la dégradation des acides nucléiques synthétisés in vitro
EP1041159A3 (fr) * 1999-03-26 2000-10-25 MIRA Diagnostika GmbH Procédé, oligonucléotides pour la dégradation des acides nucléiques synthétisés in vitro
US6509157B1 (en) * 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
WO2001036679A2 (fr) * 1999-11-15 2001-05-25 Hartwell John G METHODES DE GENERATION DE FRAGMENTS D'ADNc SIMPLE BRIN
EP1303639A2 (fr) 2000-04-14 2003-04-23 Cornell Research Foundation, Inc. Procede de conception d'un reseau adressable dans la detection de differences de sequences d'acides nucleiques, au moyen d'une reaction de detection de ligase
EP1307469B1 (fr) * 2000-08-03 2008-01-23 Boehringer Mannheim Gmbh Composes de fixation d'acides nucleiques contenant des analogues pyrazolo¬3,4-d pyrimidine de purine 2,6-diamine et leurs utilisations
CA2426540A1 (fr) * 2000-10-20 2002-07-25 Biocardia, Inc. Evaluation du niveau d'expression leucocytaire
US20020165673A1 (en) * 2001-01-19 2002-11-07 Allen Morgan Method of self-sampling and analyzing a semen sample
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) * 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en) * 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7222059B2 (en) * 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
CA2476481C (fr) 2002-02-21 2016-01-26 Asm Scientific, Inc. Amplification de la recombinase polymerase
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
EP1573056A4 (fr) * 2002-05-17 2007-11-28 Nugen Technologies Inc Procedes de fragmentation, d'etiquetage et d'immobilisation d'acides nucleiques
US7273730B2 (en) * 2002-05-24 2007-09-25 Invitrogen Corporation Nested PCR employing degradable primers
EP1606419A1 (fr) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systemes et procedes pour accroitre la production de proteines et de lait chez des bovins laitiers
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US20070248978A1 (en) * 2006-04-07 2007-10-25 Expression Diagnostics, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20060263813A1 (en) * 2005-05-11 2006-11-23 Expression Diagnostics, Inc. Methods of monitoring functional status of transplants using gene panels
US20050123956A1 (en) * 2003-09-25 2005-06-09 Affymetrix, Inc. Methods for modifying DNA for microarray analysis
AU2004311882A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
DE602005018460D1 (de) 2004-02-04 2010-02-04 Qiagen North American Holdings Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation
CA2497324A1 (fr) * 2004-02-17 2005-08-17 Affymetrix, Inc. Methodes de fragmentation et de marquage d'adn
ATE510928T1 (de) 2004-02-19 2011-06-15 Univ Alberta Leptinpromotor-polymorphismen und verwendungen davon
ATE456672T1 (de) * 2004-06-17 2010-02-15 Epigenomics Ag Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen
US7645575B2 (en) * 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US20060068430A1 (en) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Purification of biomolecules from contaminating intact nucleic acids
US7642055B2 (en) 2004-09-21 2010-01-05 Applied Biosystems, Llc Two-color real-time/end-point quantitation of microRNAs (miRNAs)
BRPI0517131B1 (pt) * 2004-12-03 2021-08-31 Human Genetic Signatures Pty Ltd Métodos para simplificar ácidos nucléicos microbiais por modificação química de citosinas
US8431347B2 (en) * 2005-05-26 2013-04-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
EP1920064B1 (fr) * 2005-07-07 2014-12-24 Quanta Biosciences, Inc. Compositions et methodes ameliorant l'efficacite de l'amplification
EP2385140B1 (fr) 2005-07-25 2014-07-16 Alere San Diego, Inc. Procédé de multiplexage de l'amplification par recombinase polymérase
WO2007030882A1 (fr) * 2005-09-14 2007-03-22 Human Genetic Signatures Pty Ltd Essai pour un état de santé
EP2458011B1 (fr) 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, procédés et kits pour l'amplification d'acides nucléiques
US20070087059A1 (en) * 2005-10-17 2007-04-19 Frank Everaerts Bioactive delivery matrix compositions and methods
WO2007123386A1 (fr) 2006-04-24 2007-11-01 Sigma Alimentos, S.A. De C.V. Procédé de détection et de quantification multiple et simultanée de pathogènes par réaction en chaîne par polymérase en temps réel
WO2007127992A2 (fr) 2006-04-28 2007-11-08 Igor Kutyavin Utilisation de désoxynucléoside triphosphates a base modifiée pour la détection d'acides nucléiques
EP2021506B1 (fr) 2006-04-28 2016-03-16 Igor Kutyavin Utilisation de produits d'amplification pcr portant des elements de structure secondaire pour ameliorer la detection d'acides nucleiques par pcr
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
US20100022403A1 (en) * 2006-06-30 2010-01-28 Nurith Kurn Methods for fragmentation and labeling of nucleic acids
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
WO2008021431A2 (fr) * 2006-08-14 2008-02-21 Xdx, Inc. Méthodes et compositions permettant de diagnostiquer et de surveiller l'état de rejet de greffe et de troubles immunitaires
WO2008140484A2 (fr) * 2006-11-09 2008-11-20 Xdx, Inc. Procédés pour diagnostiquer et surveiller l'état d'un lupus érythémateux systémique
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
FR2914317B1 (fr) * 2007-03-30 2012-12-28 Biomerieux Sa Methode de decontamination d'une solution des acides nucleiques indesirables
JP5431351B2 (ja) * 2007-11-27 2014-03-05 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド バイサルファイト修飾された核酸を増幅およびコピーするための酵素
US20100286379A1 (en) * 2007-12-05 2010-11-11 Human Genetic Signatures Pty Ltd. Bisulphite treatment of rna
EP2071034A1 (fr) * 2007-12-12 2009-06-17 bioMérieux Procédé pour le traitement d'une solution en vue d'éliminer un acide ribonucléique après amplification
EP2222850A4 (fr) * 2007-12-20 2011-12-07 Human Genetic Signatures Pty Elimination des contaminants associés à l'amplification des acides nucléiques
US8669061B2 (en) * 2008-06-26 2014-03-11 Roche Molecular Systems, Inc. Method for the prevention of carryover contamination in nucleic acid amplification technologies
US8198023B2 (en) 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
WO2010048386A1 (fr) * 2008-10-24 2010-04-29 Helicos Biosciences Corporation Procédés de préparation d’échantillon pour l’analyse d’acides nucléiques pour des acides nucléiques disponibles en quantités limitées
WO2010135310A1 (fr) 2009-05-20 2010-11-25 Biosite Incorporated Glycosylase/lyase d'adn et substrats d'endonucléase ap
ES3022638T3 (en) 2009-06-05 2025-05-28 Abbott Diagnostics Scarborough Inc Recombinase polymerase amplification reagents
EP2272976A1 (fr) 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Procédé de différentiation de brins de polynucléotide
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
US20120052495A1 (en) * 2010-08-30 2012-03-01 Samsung Techwin Co., Ltd. Oligonucleotides for detecting listeria monocytogenes and use thereof
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
WO2012054639A2 (fr) 2010-10-22 2012-04-26 T2 Biosystems, Inc. Systèmes de rmn et procédés de détection rapide d'analytes
WO2012138989A1 (fr) 2011-04-07 2012-10-11 Alere San Diego Inc. Surveillance de mélanges d'amplification de recombinase-polymérase
JP5977000B2 (ja) 2011-07-12 2016-08-24 アークレイ株式会社 核酸の増幅検出方法およびキット
JP5830286B2 (ja) 2011-07-12 2015-12-09 アークレイ株式会社 核酸の増幅検出方法およびキット
KR101912488B1 (ko) 2011-09-07 2018-10-26 휴먼 제네틱 시그너처스 피티와이 엘티디 분자 검출 분석법
CN104080958A (zh) 2011-10-19 2014-10-01 纽亘技术公司 用于定向核酸扩增和测序的组合物和方法
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
EP4372084A3 (fr) 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions et procédés pour l'enrichissement ciblé de séquences d'acides nucléiques et la génération de bibliothèques à haute efficacité
US9957549B2 (en) 2012-06-18 2018-05-01 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
BR112015003354A8 (pt) 2012-08-14 2018-01-16 10X Genomics Inc métodos e composições de microcápsula
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10174373B2 (en) 2012-10-18 2019-01-08 Idexx Laboratories, Inc. Nucleic acid amplification controls and kits and methods of use thereof
CN111793621B (zh) 2012-11-02 2024-12-10 生命技术公司 用于增强pcr特异性的新颖组合物、方法和试剂盒
CA2894694C (fr) 2012-12-14 2023-04-25 10X Genomics, Inc. Procedes et systemes pour le traitement de polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
WO2014144092A1 (fr) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Séquençage séquentiel
GB201312995D0 (en) 2013-07-19 2013-09-04 Atlas Genetics Ltd Methods and kits for specific nucleic acid amplification and detection
WO2015073711A1 (fr) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions et procédés pour l'identification d'une lecture de séquençage en double
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US10196685B2 (en) 2014-05-13 2019-02-05 Rasirc, Inc. Methods and systems for delivering process gases to critical process applications
JP2017522866A (ja) 2014-06-26 2017-08-17 １０エックス ジェノミクス， インコーポレイテッド 核酸配列の分析
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3889325A1 (fr) 2014-06-26 2021-10-06 10X Genomics, Inc. Procédés d'analyse d'acides nucléiques provenant de cellules individuelles ou de populations de cellules
EP3212807B1 (fr) 2014-10-29 2020-09-02 10X Genomics, Inc. Procédés et compositions de séquençage ciblé d'acides nucléiques
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10221436B2 (en) 2015-01-12 2019-03-05 10X Genomics, Inc. Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
WO2016137973A1 (fr) 2015-02-24 2016-09-01 10X Genomics Inc Procédés et systèmes de traitement de cloisonnement
CN115651972A (zh) 2015-02-24 2023-01-31 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
CN107429296B (zh) 2015-03-13 2022-01-28 生命技术公司 捕获、检测和定量小rna的方法、组合物与试剂盒
CN107614699A (zh) * 2015-04-17 2018-01-19 加利福尼亚大学董事会 用于检测凝集的方法以及用于实践该方法的组合物
ES2961374T3 (es) 2015-04-24 2024-03-11 Atila Biosystems Incorporated Amplificación con cebadores de composición de nucleótidos limitada
KR101605671B1 (ko) 2015-05-12 2016-03-23 주식회사 엔지노믹스 데옥시유리딘을 포함하는 폴리뉴클레오타이드를 이용한 핵산 증폭반응의 위양성 여부 확인 방법
WO2017096158A1 (fr) 2015-12-04 2017-06-08 10X Genomics, Inc. Procédés et compositions pour l'analyse d'acide nucléique
WO2017197338A1 (fr) 2016-05-13 2017-11-16 10X Genomics, Inc. Systèmes microfluidiques et procédés d'utilisation
EP3279337A1 (fr) 2016-08-04 2018-02-07 Servizo Galego de Saúde (SERGAS) Utilisation de sondes courtes entre 8 et 9 nucléotides dans des biotests multiplex
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (fr) 2017-01-30 2018-08-02 10X Genomics, Inc. Procédés et systèmes de codage à barres de cellules individuelles sur la base de gouttelettes
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4230746A3 (fr) 2017-05-26 2023-11-01 10X Genomics, Inc. Analyse de cellule unique de chromatine accessible par transposase
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10155939B1 (en) * 2017-06-15 2018-12-18 New England Biolabs, Inc. Method for performing multiple enzyme reactions in a single tube
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP3954782A1 (fr) 2017-11-15 2022-02-16 10X Genomics, Inc. Perles de gel fonctionnalisées
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
MX2018008742A (es) 2018-07-16 2020-01-17 Sigma Alimentos Sa De Cv Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas.
US20220389497A1 (en) 2019-11-04 2022-12-08 Mirnax Biosens, S.L. Bivalent reverse primer
EP3901286A1 (fr) 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Amorce inverse bivalente
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
US20240207805A1 (en) * 2021-04-30 2024-06-27 Nutcracker Therapeutics, Inc. METHODS FOR PERFORMING IN VITRO TRANSCRIPTION USING dUTP
WO2024146937A1 (fr) 2023-01-06 2024-07-11 Dna Script Procédés d'obtention d'acides nucléiques correctement assemblés

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989011548A1 (fr) * 1988-05-20 1989-11-30 Cetus Corporation Sondes specifiques a des sequences immobilisees
EP0401037A2 (fr) * 1989-06-01 1990-12-05 Life Technologies Inc. Procédé pour contrôler la contamination dans l'amplification des acides nucléiques
EP0415755A2 (fr) * 1989-09-01 1991-03-06 Life Technologies Inc. Procédé pour contrôler la contamination des réactions d'amplification des acides nucléiques dépendant des oligonucléotides

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4873192A (en) * 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
CA1340807C (fr) * 1988-02-24 1999-11-02 Lawrence T. Malek Procede d'amplification d'une sequence d'acide nucleique
CA2005589C (fr) * 1988-12-16 2001-02-06 Thomas Raymond Gingeras Systeme de replication de sequences auto-entretenu

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989011548A1 (fr) * 1988-05-20 1989-11-30 Cetus Corporation Sondes specifiques a des sequences immobilisees
EP0401037A2 (fr) * 1989-06-01 1990-12-05 Life Technologies Inc. Procédé pour contrôler la contamination dans l'amplification des acides nucléiques
EP0415755A2 (fr) * 1989-09-01 1991-03-06 Life Technologies Inc. Procédé pour contrôler la contamination des réactions d'amplification des acides nucléiques dépendant des oligonucléotides

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Biochemistry, vol. 22, 1983, American Chemical Society, D. SAGHER et al.: "Insertion of nucleotides opposite apurinic/apyrimidinic sites in deoxyribonucleic acid during in vitro synthesis: Uniquencess of adenine nucleotides", pages 4518-4526, see abstract; page 4520, column 2, lines 1-8 (cited in the application) *
Biotechnology, vol. 6, October 1988, P.M. LIZARDI et al.: "Exponential amplification of recombinant-RNA hybridization probes", pages 1197-1202, see abstract (cited in the application) *
Gene, vol. 93, 1 September 1990, Elsevier Science Publishers B.V. (Biomedical Division), M.C. LONGO et al.: "Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions", pages 125-128, see the whole document *
Genomics, vol. 4, 1989, Academic Press, Inc., D.Y. WU et al.: "The ligation amplification reaction (LAR)-amplification of sepcific DNA sequences using sequential rounds of template-dependent ligation", pages 560-569, see abstract (cited in the application) *
Proceedings of the National Academy of Sciences of USA, vol. 82, January 1985, T.A. KUNKEL: "Rapid and efficient site-specific mutagenesis without phenotypic selection", pages 488-492, see page 489, lines 18-41 (cited in the application) *
Proceedings of the National Academy of Sciences of USA, vol. 86, February 1989, D.Y. KWOH et al.: "Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format", pages 1172-1177, see abstract (cited in the application) *
Proceedings of the National Academy of Sciences of USA, vol. 87, March 1990, J.C. GUATELLI et al.: "Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication", pages 1874-1878, see abstract (cited in the application) *

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JP3392863B2 (ja) 2003-03-31
CA2087724C (fr) 2003-09-16
DE69130800D1 (de) 1999-03-04
WO1992001814A2 (fr) 1992-02-06
EP0540693B1 (fr) 1999-01-20
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GR3029987T3 (en) 1999-07-30
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ES2128323T3 (es) 1999-05-16
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AU665338B2 (en) 1996-01-04
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AU8532791A (en) 1992-02-18
ATE176002T1 (de) 1999-02-15

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