WO1991018110A1 - Procede et systeme pour effectuer des reactions biochimiques - Google Patents
Procede et systeme pour effectuer des reactions biochimiques Download PDFInfo
- Publication number
- WO1991018110A1 WO1991018110A1 PCT/SE1991/000343 SE9100343W WO9118110A1 WO 1991018110 A1 WO1991018110 A1 WO 1991018110A1 SE 9100343 W SE9100343 W SE 9100343W WO 9118110 A1 WO9118110 A1 WO 9118110A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- capillary
- reagent
- reaction vessel
- bore
- reagents
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000005842 biochemical reaction Methods 0.000 title claims abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 77
- 238000006243 chemical reaction Methods 0.000 claims abstract description 63
- 239000012530 fluid Substances 0.000 claims abstract description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000001681 protective effect Effects 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 108091034117 Oligonucleotide Proteins 0.000 claims description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 claims description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 claims description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 claims description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000002405 diagnostic procedure Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 description 16
- 239000000243 solution Substances 0.000 description 6
- 230000003321 amplification Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 241000725303 Human immunodeficiency virus Species 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000011109 contamination Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- NSMXQKNUPPXBRG-SECBINFHSA-N (R)-lisofylline Chemical compound O=C1N(CCCC[C@H](O)C)C(=O)N(C)C2=C1N(C)C=N2 NSMXQKNUPPXBRG-SECBINFHSA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000009429 distress Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- -1 paraffines Substances 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000002901 radioactive waste Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
Definitions
- the present invention relates to a method to perform biochemical reactions and a combination of a capillary and a reaction vessel for use in said method.
- the invention is applicable for all small volume biochemical reactions in which the reagents cannot be mixed beforehand. Particularly, the invention is intended for the PCR (Polymerase Chain Reaction)-technique.
- RNA DNA
- ELISA Enzyme Linked ImmunoSorbent
- a person may, however, be HIV-positive without antibodies being present if he/she, for instance, is in the early stages of the disease. In this case the ELISA test gives a negative result and the person concerned then risks unwittingly transmitting the infection to others. Therefore the need for a better, i.e. more sensitive, HIV test is very great.
- the diagnosis of other viruses also, the culture of which previously took a long time, has been improved with the PCR technique.
- PCR diagnosis comprises three stages:
- stage 1) is time-consuming and demanding work, primarily because the reagents cannot be mixed in advance, and thus give rise to many sources of error. It is very important that stage 1) should be carried out with great care and precision because the amplification in stage 2) and the detection result in stage 3) depend absolutely on the reliability of stage 1).
- the object of the invention was to deminish the contamination risk as well as the time required to prepare small reagent volumes for a specific biochemical reaction in which the reagents cannot be mixed in advance and the preparation is time-consuming.
- a capillary which contains several reagents separated by intermediate hydrophobic liquid, e.g. paraffines, oils, alkanes.
- reagent storage as well as sample reaction takes place.
- the sample is added to the capillary and then the capillary is melted at one end.
- the mixing of the sample with the reagents is done in that a steel pin is put into the capillary and a magnet is moved in an upward and downward direction along the outside of the capillary.
- the capillary is centrifugated to obtain the reaction solution and the hydrophobic liquid in two separate phases.
- the capillary has to be cut at the sealed end and also at the boundary between hydrophobic liquid-reaction solution and thereafter the reaction solution is transferred to a cuvette or the like, for measurement of, for example, UV absorbance.
- Fig. 1 is a diagrammatic view if a reaction vessel including a reagent capillary containing reagent
- Fig. 2 is a diagrammatic view of an alternative embodiment of a reaction vessel including an alternative embodiment of a reagent capillary;
- Fig. 3 is a plan view of the embodiment shown in Fig. 2;
- Fig. 4 shows the reagent capillary depicted in Fig. 1 on a larger scale
- Fig. 5 shows the reagent capillary depicted in Fig. 2 on a larger scale.
- Fig. 1 shows a ready-prepared reaction vessel 1 according to the present invention.
- a reagent capillary 3 Inside the reaction vessel 1, for instance an Eppendorf tube, is placed a reagent capillary 3.
- the reagent capillary 3 is provided with different reagents, which can be of any suitable type for a desired reaction.
- In the bottom of the reaction vessel 1 there may be water or buffer 2 for subsequent dilution of the reagents.
- Fig. 2 shows an alternative and preferred embodiment of a reaction vessel 1 and a reagent capillary 3.
- the reaction vessel 1 is provided with a lid 4 having a bore 4a.
- the bore 4a is covered by a permeable membrane 4b.
- the bore 4a fitted with a membrane is located centrally in the lid 4 in the shown em ⁇ bodiment but this is not a critical feature. In fact it is possible to provide the lid with several bores to be able to put in more than one reagent capillary as desired.
- the bore 4a forms a stop collar for the reagent capillary 3 in accordance with Fig. 5, which is described in greater detail below.
- the reagent capillary 3 depicted in Fig. 4 is designed to be inserted into the reagent vessel 1 shown in Fig. 1.
- the reagent capillary 3 is provided with different reagents 6-13 for a specific biochemical reaction. The amount of each reagent is calculated and intended only for this specific reaction. If a PCR reaction is to be performed the reagent solutions 6-13 comprise PCR buffer, dCTP, dGTP, dATP, dTTP, two or more oligonucleotides, all of the reagents being calculated for a specific PCR reaction, and termostable DNA polymerase. Between the reagents there is air or an inert fluid. Naturally, the mutual order is optional.
- a modified reagent capillary is depicted in Fig. 5.
- This reagent capillary is designed to be inserted into a reaction vessel according to Fig. 2.
- the reagent capillary differs from the reagent capillary shown in Fig. 4 in that there is an annular locking groove 5 on the lower part of the capillary intended to be snapped into the bore 4a.
- a protective cover 15 is fitted over the upper end of the capillary.
- the reaction vessel according to Fig. 2 and the reagent capillary according to Fig. 5 are stored separately until use.
- the lower end of the capillary 3 is pushed through the permeable membrane 4b in the lid 4 of the reaction vessel 1, whereupon the locking groove 5 engages with the stop collar formed by the bore 4a.
- the protec ⁇ tive cover 15 protects the contents of the reagent capillary 3 from contamination during the process of insertion and pushing into the reaction vessel 1.
- the reagent solutions in the reagent capillary 3 have thawed, they are then centrifuged down and mixed with one another and, where applicable, with the diluent 2 at the bottom of the vessel 1. After centrifuging, the lid 4 may be opened without having to remove the capillary 3 from the lid.
- the advantage of this is that material can readily be added to or extracted from the reaction vessel if desired.
- reagent capillaries After producing the reagent capillaries, i.e. by aspirating the different reagents with air or inert fluid in between, either manually or automatically, they may be packed separately or placed in a reaction vessel in kits for performing a specific biochemical reaction. Of course, this packaging takes place under sterile conditions.
- An alternative method of producing the capillaries would be to aspirate the reagents into capillaries with air or inert fluid between the reagents, freeze the capillaries, cut the capillaries in the air sections, and to place the desired capillary pieces in one common outer capillary having an inner diameter correspon ⁇ ding to the outer diameter of the capillary pieces. This would allow combining of the reagents in any desired way.
- the reagent capillaries with or without the reaction vessels are stored in the frozen state until use.
- the reagent capillary is thawed and the contents thereof are centrifuged down in the reaction vessel, being mixed with each other and with diluent if any.
- all the reagents, including heat stable DNA polymerase are now in the reaction vessel and the only further addition needed before the amplification is of the sample, e.g. blood.
- the sample is added by using a dosing system described in applicants pending Swedish patent application SE 91 0726-0. Briefly, the sample is drawn up into a capillary, of the same type as reaction capillary 3, by the capillary effects. Thereaf ⁇ ter the sample capillary is inserted in an unoccupied bore 4a in the lid 4 of the reaction vessel 1 which thereafter is once again centrifugated.
- the present inventor suggest using material for the reaction vessels that does not or only slightly absorb UV light. If ethidium bromide is added after the reaction it would then be possible to detect whether or not DNA has been amplified by viewing the vessels under UV light with the naked eye. Preferably the ethidium bromide addition is made in the same manner as the above described sample addition.
- reagent capillaries 3 have been prepared for a specific sample volume and a specific biochemical reaction. Other reactions require different volumes and number of reagents.
- kits i.e. complete sets containing reagents for a specific reaction.
- kits usually consist of Eppendorf tubes containing different reagents suitable for about 100 standard reactions.
- For each reaction a certain volume is mixed from each tube.
- one kit can contain, e.g. 500 capillaries, each ready to use for the reaction it is designed for.
- kits based in the reagent capillaries desribed in the present application is that the user does not have to pipette the reagent and is able, instead, to select the appropriate reagent capillary for the relevant reaction with fingers of tweezers.
- the simplification of the work is obvious, above all in regard to the handling of radioactive reagents, as there is no risk of contaminating pipettes, less risk of radioactive waste and shorter periods of exposure for the staff.
- reaction capillaries in PCR technology there is the saving in time and the benefits of worker protection in many biotech- nological sectors.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Devices For Use In Laboratory Experiments (AREA)
Abstract
Cette invention concerne un procédé permettant d'effectuer des réactions biochimiques ainsi que l'association d'un tube capillaire (3) et d'un réacteur (1) utlisée dans ce procédé. Les tubes capillaires (3) de réactifs renferment des réactifs gelés (6-13) séparés les uns des autres par de l'air ou un liquide inerte. Lors de l'utilisation, on place les tubes capillaires de réactifs (3) dans le réacteur (1) afin qu'ils dégèlent puis le contenu est centrifugé vers le fond du réacteur (1). Cette invention peut être utilisée pour tous les types de réactions biochimiques courantes et pour tous les types de tests de diagnostic dans lesquels on ne peut mélanger à l'avance les réactifs. Ce procédé est tout particulièrement adapté aux diagnostics selon la technique de l'amplification enzymatique du génome, il est également très utile lorsqu'il s'agit de manipuler des réacitfs radioactifs, par exemple, des nucléotides marqués, au niveau de réactions de séquençage,etc.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU79715/91A AU660615B2 (en) | 1990-05-16 | 1991-05-15 | Method and means to perform biochemical reactions |
| EP91910180A EP0530283B1 (fr) | 1990-05-16 | 1991-05-15 | Procede et systeme pour effectuer des reactions biochimiques |
| RU9192016293A RU2082754C1 (ru) | 1990-05-16 | 1991-05-15 | Способ проведения биохимических реакций и устройство для его осуществления |
| DE69124236T DE69124236T2 (de) | 1990-05-16 | 1991-05-15 | Verfahren und vorrichtung zur ausführung biochemischer reaktionen |
| CA002082933A CA2082933C (fr) | 1990-05-16 | 1991-05-15 | Methode et moyens mis en oeuvre pour obtenir des reactions biochimiques |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9001772-4 | 1990-05-16 | ||
| SE9001772A SE465086B (sv) | 1990-05-16 | 1990-05-16 | Reagenskapillaerer, reaktionskaerl, beredningssaett och anvaendning daerav |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1991018110A1 true WO1991018110A1 (fr) | 1991-11-28 |
Family
ID=20379508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/SE1991/000343 WO1991018110A1 (fr) | 1990-05-16 | 1991-05-15 | Procede et systeme pour effectuer des reactions biochimiques |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0530283B1 (fr) |
| JP (1) | JPH0646936B2 (fr) |
| AT (1) | ATE147789T1 (fr) |
| AU (1) | AU660615B2 (fr) |
| CA (1) | CA2082933C (fr) |
| DE (1) | DE69124236T2 (fr) |
| RU (1) | RU2082754C1 (fr) |
| SE (1) | SE465086B (fr) |
| WO (1) | WO1991018110A1 (fr) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993015222A1 (fr) * | 1992-01-29 | 1993-08-05 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Procede pour eviter les risques de contamination, notamment applicable aux techniques d'amplification de l'adn ou de l'arn |
| WO1993016200A1 (fr) * | 1992-02-13 | 1993-08-19 | Kosak Kenneth M | REACTIFS THERMO-LIBERABLES DESTINES AUX REACTIONS CHIMIQUES $i (IN VITRO) |
| EP0572057A1 (fr) * | 1992-05-11 | 1993-12-01 | Johnson & Johnson Clinical Diagnostics, Inc. | Composition et kit de réactifs de PCR et procédés pour amplification et détection avec amplification non-spécifique d'acides nucléiques réduite |
| WO1995019447A1 (fr) * | 1994-01-14 | 1995-07-20 | The Jockey Club | Procede d'echantillonnage non invasif pour analyse d'acides nucleiques |
| WO1997048491A1 (fr) * | 1996-06-20 | 1997-12-24 | Hamilton Bonaduz Ag | Procede pour effectuer des reactions chimiques, notamment biochimiques, et pointe de pipette avec recipient reactionnel, eventuellement avec element de reception supplementaire pour la pointe de pipette |
| WO1998054292A1 (fr) * | 1997-05-28 | 1998-12-03 | Alphahelix Ab | Nouvelle cuve a reaction et ses procedes d'utilisation |
| ES2153745A1 (es) * | 1998-07-31 | 2001-03-01 | Ivia | Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado. |
| US6511814B1 (en) | 1999-03-26 | 2003-01-28 | Idexx Laboratories, Inc. | Method and device for detecting analytes in fluids |
| US6551842B1 (en) | 1999-03-26 | 2003-04-22 | Idexx Laboratories, Inc. | Method and device for detecting analytes in fluids |
| US6602719B1 (en) | 1999-03-26 | 2003-08-05 | Idexx Laboratories, Inc. | Method and device for detecting analytes in fluids |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0838025B1 (fr) * | 1995-07-13 | 2007-04-25 | Applera Corporation | Appareil autonome integrant l'extraction, l'amplification et la detection de l'acide nucleique |
| EP2259070A3 (fr) | 1995-07-31 | 2011-03-30 | Precision System Science Co., Ltd. | Récipient |
| JP5899624B2 (ja) * | 2011-02-18 | 2016-04-06 | セイコーエプソン株式会社 | 反応容器 |
| WO2012176598A1 (fr) * | 2011-06-24 | 2012-12-27 | 株式会社島津製作所 | Procédé pour séparer une substance reçue dans un récipient |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD225788A1 (de) * | 1984-04-25 | 1985-08-07 | Univ Berlin Humboldt | Mikrotest zur durchfuehrung analytischer bestimmungen |
-
1990
- 1990-05-16 SE SE9001772A patent/SE465086B/sv unknown
-
1991
- 1991-05-15 CA CA002082933A patent/CA2082933C/fr not_active Expired - Fee Related
- 1991-05-15 RU RU9192016293A patent/RU2082754C1/ru active
- 1991-05-15 DE DE69124236T patent/DE69124236T2/de not_active Expired - Fee Related
- 1991-05-15 AU AU79715/91A patent/AU660615B2/en not_active Ceased
- 1991-05-15 AT AT91910180T patent/ATE147789T1/de active
- 1991-05-15 WO PCT/SE1991/000343 patent/WO1991018110A1/fr active IP Right Grant
- 1991-05-15 JP JP3110423A patent/JPH0646936B2/ja not_active Expired - Fee Related
- 1991-05-15 EP EP91910180A patent/EP0530283B1/fr not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD225788A1 (de) * | 1984-04-25 | 1985-08-07 | Univ Berlin Humboldt | Mikrotest zur durchfuehrung analytischer bestimmungen |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993015222A1 (fr) * | 1992-01-29 | 1993-08-05 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Procede pour eviter les risques de contamination, notamment applicable aux techniques d'amplification de l'adn ou de l'arn |
| WO1993016200A1 (fr) * | 1992-02-13 | 1993-08-19 | Kosak Kenneth M | REACTIFS THERMO-LIBERABLES DESTINES AUX REACTIONS CHIMIQUES $i (IN VITRO) |
| US5413924A (en) * | 1992-02-13 | 1995-05-09 | Kosak; Kenneth M. | Preparation of wax beads containing a reagent for release by heating |
| US5643764A (en) * | 1992-02-13 | 1997-07-01 | Kosak; Kenneth M. | Reactions using heat-releasable reagents in wax beads |
| EP0572057A1 (fr) * | 1992-05-11 | 1993-12-01 | Johnson & Johnson Clinical Diagnostics, Inc. | Composition et kit de réactifs de PCR et procédés pour amplification et détection avec amplification non-spécifique d'acides nucléiques réduite |
| WO1995019447A1 (fr) * | 1994-01-14 | 1995-07-20 | The Jockey Club | Procede d'echantillonnage non invasif pour analyse d'acides nucleiques |
| WO1997048491A1 (fr) * | 1996-06-20 | 1997-12-24 | Hamilton Bonaduz Ag | Procede pour effectuer des reactions chimiques, notamment biochimiques, et pointe de pipette avec recipient reactionnel, eventuellement avec element de reception supplementaire pour la pointe de pipette |
| WO1998054292A1 (fr) * | 1997-05-28 | 1998-12-03 | Alphahelix Ab | Nouvelle cuve a reaction et ses procedes d'utilisation |
| US6451258B1 (en) | 1997-05-28 | 2002-09-17 | Alphahelix Ab | Reaction vessel, cassette and system for performing biochemical reactions |
| ES2153745A1 (es) * | 1998-07-31 | 2001-03-01 | Ivia | Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado. |
| US6511814B1 (en) | 1999-03-26 | 2003-01-28 | Idexx Laboratories, Inc. | Method and device for detecting analytes in fluids |
| US6551842B1 (en) | 1999-03-26 | 2003-04-22 | Idexx Laboratories, Inc. | Method and device for detecting analytes in fluids |
| US6602719B1 (en) | 1999-03-26 | 2003-08-05 | Idexx Laboratories, Inc. | Method and device for detecting analytes in fluids |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0530283B1 (fr) | 1997-01-15 |
| AU660615B2 (en) | 1995-07-06 |
| JPH04228100A (ja) | 1992-08-18 |
| ATE147789T1 (de) | 1997-02-15 |
| JPH0646936B2 (ja) | 1994-06-22 |
| DE69124236D1 (de) | 1997-02-27 |
| RU2082754C1 (ru) | 1997-06-27 |
| CA2082933A1 (fr) | 1991-11-17 |
| SE465086B (sv) | 1991-07-22 |
| EP0530283A1 (fr) | 1993-03-10 |
| DE69124236T2 (de) | 1997-08-07 |
| SE9001772D0 (sv) | 1990-05-16 |
| CA2082933C (fr) | 2002-09-17 |
| AU7971591A (en) | 1991-12-10 |
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