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WO1991016434A3 - Bacillus thuringiensis cryif and cryix genes and proteins toxic to lepidopteran insects - Google Patents

Bacillus thuringiensis cryif and cryix genes and proteins toxic to lepidopteran insects Download PDF

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Publication number
WO1991016434A3
WO1991016434A3 PCT/US1991/002560 US9102560W WO9116434A3 WO 1991016434 A3 WO1991016434 A3 WO 1991016434A3 US 9102560 W US9102560 W US 9102560W WO 9116434 A3 WO9116434 A3 WO 9116434A3
Authority
WO
WIPO (PCT)
Prior art keywords
cryix
cryif
lepidopteran insects
designated
genes
Prior art date
Application number
PCT/US1991/002560
Other languages
French (fr)
Other versions
WO1991016434A2 (en
Inventor
M Cynthia Gawron-Burke
Judith A Chambers
Jose M Gonzalez Jr
Original Assignee
Ecogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecogen Inc filed Critical Ecogen Inc
Priority to CA002080684A priority Critical patent/CA2080684C/en
Publication of WO1991016434A2 publication Critical patent/WO1991016434A2/en
Publication of WO1991016434A3 publication Critical patent/WO1991016434A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Pest Control & Pesticides (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Two purified and isolated cryI-type genes were obtained from a novel B.t. strain. One gene, designated cryIF (SEQ ID NO:1), has a nucleotide base sequence coding for the amino acid sequence illustrated in Figure 1. The 134 kDa crystal protein, designated CryIF (SEQ ID NO:2), produced by this gene is toxic to European corn borer larvae and other lepidopteran insects. The second gene, designated cryIX, produces a crystal portein of about 81 kDa, designated CryIX, that is also toxic to lepidopteran insects.
PCT/US1991/002560 1990-04-16 1991-04-15 BACILLUS THURINGIENSIS cryIF AND cryIX GENES AND PROTEINS TOXIC TO LEPIDOPTERAN INSECTS WO1991016434A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002080684A CA2080684C (en) 1990-04-16 1991-04-15 Bacillus thuringiensis cryif and cryix genes and proteins toxic to lepidopteran insects

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51032790A 1990-04-16 1990-04-16
US510,327 1990-04-16

Publications (2)

Publication Number Publication Date
WO1991016434A2 WO1991016434A2 (en) 1991-10-31
WO1991016434A3 true WO1991016434A3 (en) 1991-12-12

Family

ID=24030295

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/002560 WO1991016434A2 (en) 1990-04-16 1991-04-15 BACILLUS THURINGIENSIS cryIF AND cryIX GENES AND PROTEINS TOXIC TO LEPIDOPTERAN INSECTS

Country Status (5)

Country Link
EP (1) EP0528823A1 (en)
AU (1) AU647121B2 (en)
CA (1) CA2080684C (en)
TR (1) TR26973A (en)
WO (1) WO1991016434A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5686069A (en) * 1990-10-15 1997-11-11 Mycogen Corporation Protein toxins active against lepidopteran pests
TW261517B (en) * 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5356623A (en) * 1993-03-17 1994-10-18 Ecogen Inc. Bacillus thuringiensis cryET1 toxin gene and protein toxic to lepidopteran insects
US5849870A (en) * 1993-03-25 1998-12-15 Novartis Finance Corporation Pesticidal proteins and strains
HU220714B1 (en) * 1993-03-25 2002-04-29 Novartis Ag. New pesticide proteins and strains
US5877012A (en) * 1993-03-25 1999-03-02 Novartis Finance Corporation Class of proteins for the control of plant pests
US5322687A (en) * 1993-07-29 1994-06-21 Ecogen Inc. Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects
US5508264A (en) * 1994-12-06 1996-04-16 Mycogen Corporation Pesticidal compositions
ES2330168T3 (en) * 1995-10-13 2009-12-04 Dow Agrosciences Llc MOPDIFIED GENE OF BACILLUS THURINGIENSIS TO COMBAT LEPIDOPTERS IN PLANTS.
US6369213B1 (en) 1996-07-01 2002-04-09 Mycogen Corporation Toxins active against pests
EP0909324A2 (en) 1996-07-01 1999-04-21 Mycogen Corporation Toxins active against pests
US5965428A (en) * 1996-10-08 1999-10-12 Ecogen, Inc. Chimeric lepidopteran-toxic crystal proteins
IL132039A0 (en) 1997-04-03 2001-03-19 Novartis Ag Plant pest control
AR025349A1 (en) * 1999-08-23 2002-11-20 Mycogen Corp METHODS TO CONTROL GRAY WORM PESTS
US11130964B2 (en) 2014-11-20 2021-09-28 Monsanto Technology Llc Insect inhibitory proteins

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0358557A2 (en) * 1988-09-06 1990-03-14 Plant Genetic Systems, N.V. Plants transformed with a DNA sequence from bacillus thuringiensis lethal to lepidoptera
WO1990013651A1 (en) * 1989-05-09 1990-11-15 Imperial Chemical Industries Plc Bacterial genes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU624349B2 (en) * 1987-11-05 1992-06-11 David M. Gjerdrum Device for monitoring radon exposure
GB8800652D0 (en) * 1988-01-13 1988-02-10 Ici Plc Bacterial strain

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0358557A2 (en) * 1988-09-06 1990-03-14 Plant Genetic Systems, N.V. Plants transformed with a DNA sequence from bacillus thuringiensis lethal to lepidoptera
WO1990013651A1 (en) * 1989-05-09 1990-11-15 Imperial Chemical Industries Plc Bacterial genes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Journal of Cellular Biochemistry, Suppl. 14E, 1990, abstract R206, J.A. Chambers et al.: "Isolation and characterization of novel delta-endotoxin genes from bacillus thuringiensis subsp. aizawai", page 296, see the whole abstract *

Also Published As

Publication number Publication date
CA2080684C (en) 2010-02-02
AU7687391A (en) 1991-11-11
WO1991016434A2 (en) 1991-10-31
CA2080684A1 (en) 1991-10-17
AU647121B2 (en) 1994-03-17
TR26973A (en) 1994-09-12
EP0528823A1 (en) 1993-03-03

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