WO1991011531A1 - SONDE D'HYBRIDATION POUR LA DETECTION DES SOUCHES DE $i(BRANHAMELLA CATARRHALIS) - Google Patents
SONDE D'HYBRIDATION POUR LA DETECTION DES SOUCHES DE $i(BRANHAMELLA CATARRHALIS) Download PDFInfo
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- WO1991011531A1 WO1991011531A1 PCT/EP1991/000211 EP9100211W WO9111531A1 WO 1991011531 A1 WO1991011531 A1 WO 1991011531A1 EP 9100211 W EP9100211 W EP 9100211W WO 9111531 A1 WO9111531 A1 WO 9111531A1
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- Prior art keywords
- probes
- group
- hybridization
- branhamella catarrhalis
- probe
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Definitions
- Probes targeting rRNA are advantageous over probes which target the genomic DNA such as the probe described by Beaulieu and Roy, since rRNA is single- stranded and consequently directly available for hybridization, and is present in multiple copies in bacterial cells.
- the invention thus provides for probes which are either replicas (those designated by numbers followed by "IC” or “ICR”) in terms of nucleotide sequence of sequences contained in the RNAs or DNAs of most Branhamella catarrhalis strains with occasionally a few insignificant differences in nucleotide sequences or formed of sequences, those designated by bare numbers or by numbers followed by "R”, complementary to sequences included in the natural RNAs of Branhamella catarrhalis.
- the 200 ⁇ l hybridization mix containing 2 pmol digoxigenine labeled probe BCl was added and hybridization was done at 60"C for 1 h. Washing was performed at the same stringency for 20 min.
- the detection protocol as described in the Boehringer labeling and detection kit was followed except that paranitrophenyl-phosphate (3.6 mg/ l) was used as substrate for alkaline phosphatase.
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne des sondes d'hybridation servant à détecter les souches de Branhamella catarrhalis. Les sondes faisant l'objet de l'invention sont caractérisées par les séquences de nucléotides (I) suivantes:
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB90400297.9 | 1990-02-02 | ||
| EP90400297 | 1990-02-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1991011531A1 true WO1991011531A1 (fr) | 1991-08-08 |
Family
ID=8205679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/EP1991/000211 WO1991011531A1 (fr) | 1990-02-02 | 1991-02-01 | SONDE D'HYBRIDATION POUR LA DETECTION DES SOUCHES DE $i(BRANHAMELLA CATARRHALIS) |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU7188491A (fr) |
| CA (1) | CA2075186A1 (fr) |
| WO (1) | WO1991011531A1 (fr) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0737085A1 (fr) * | 1993-09-29 | 1996-10-16 | The Research Foundation Of State University Of New York At Buffalo | VACCIN CONTRE $i(BRANHAMELLA CATARRHALIS) |
| EP0759777A1 (fr) * | 1994-05-17 | 1997-03-05 | The Research Foundation Of State University Of New York At Buffalo | VACCIN CONTRE $i(MORAXELLA CATARRHALIS) |
| US5994066A (en) * | 1995-09-11 | 1999-11-30 | Infectio Diagnostic, Inc. | Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US6001564A (en) * | 1994-09-12 | 1999-12-14 | Infectio Diagnostic, Inc. | Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US6294667B1 (en) | 1996-10-07 | 2001-09-25 | Amersham International Plc | Analysis of carbohydrates |
| US7943346B2 (en) | 1994-09-12 | 2011-05-17 | Geneohm Sciences Canada Inc. | Probes and primers for detection of bacterial pathogens and antibiotic resistance genes |
| US8034588B2 (en) | 1997-11-04 | 2011-10-11 | Geneohm Sciences Canada Inc. | Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories |
| US8114601B2 (en) | 1999-09-28 | 2012-02-14 | Geneohm Sciences Canada Inc. | Highly conserved genes and their use to generate probes and primers for detection of microorganisms |
| US8426137B2 (en) | 1996-11-04 | 2013-04-23 | Genohm Sciences Canada, Inc. | Methods and probes for detecting a vancomycin resistance gene |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0079139A1 (fr) * | 1981-10-16 | 1983-05-18 | Orion-yhtymä Oy | Méthode et combinaison de réactifs pour l'identification des micro-organismes et l'utilisation de la hybridisation en sandwich des acides nucléiques à cette fin |
-
1991
- 1991-02-01 AU AU71884/91A patent/AU7188491A/en not_active Abandoned
- 1991-02-01 WO PCT/EP1991/000211 patent/WO1991011531A1/fr active Application Filing
- 1991-02-01 CA CA002075186A patent/CA2075186A1/fr not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0079139A1 (fr) * | 1981-10-16 | 1983-05-18 | Orion-yhtymä Oy | Méthode et combinaison de réactifs pour l'identification des micro-organismes et l'utilisation de la hybridisation en sandwich des acides nucléiques à cette fin |
Non-Patent Citations (3)
| Title |
|---|
| Abstracts of the 89th Annual Meeting of the American Society for Microbology, New Orleans LA, 14th-18th May 1989 vol. 89(D) D. Beaulieu et al.: "Construction of a species-specific DNA probe for Branhamella catarrhalis", see page 124 * |
| Journal of General Microbology, vol. 135, 1989 SGM (Colchester, GB) R.Rosseau et al.: "Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA", pages 1735-1745 * |
| Nucleic Acids Research, vol. 17, no. 19, 11 october 1989, IRL Press (Oxford, GB) U. Edwards et al.: "Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16s ribosomal RNA", pages 7843-7853 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0737085A4 (fr) * | 1993-09-29 | 1999-04-07 | Univ New York State Res Found | VACCIN CONTRE -i(BRANHAMELLA CATARRHALIS) |
| EP0737085A1 (fr) * | 1993-09-29 | 1996-10-16 | The Research Foundation Of State University Of New York At Buffalo | VACCIN CONTRE $i(BRANHAMELLA CATARRHALIS) |
| EP0759777A1 (fr) * | 1994-05-17 | 1997-03-05 | The Research Foundation Of State University Of New York At Buffalo | VACCIN CONTRE $i(MORAXELLA CATARRHALIS) |
| EP0759777A4 (fr) * | 1994-05-17 | 1998-12-02 | Univ New York State Res Found | VACCIN CONTRE -i(MORAXELLA CATARRHALIS) |
| US7943346B2 (en) | 1994-09-12 | 2011-05-17 | Geneohm Sciences Canada Inc. | Probes and primers for detection of bacterial pathogens and antibiotic resistance genes |
| US6001564A (en) * | 1994-09-12 | 1999-12-14 | Infectio Diagnostic, Inc. | Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US5994066A (en) * | 1995-09-11 | 1999-11-30 | Infectio Diagnostic, Inc. | Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US6294667B1 (en) | 1996-10-07 | 2001-09-25 | Amersham International Plc | Analysis of carbohydrates |
| US8426137B2 (en) | 1996-11-04 | 2013-04-23 | Genohm Sciences Canada, Inc. | Methods and probes for detecting a vancomycin resistance gene |
| US8034588B2 (en) | 1997-11-04 | 2011-10-11 | Geneohm Sciences Canada Inc. | Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories |
| US8067207B2 (en) | 1997-11-04 | 2011-11-29 | Geneohm Sciences Canada Inc. | Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories |
| US8114601B2 (en) | 1999-09-28 | 2012-02-14 | Geneohm Sciences Canada Inc. | Highly conserved genes and their use to generate probes and primers for detection of microorganisms |
| US8182996B2 (en) | 1999-09-28 | 2012-05-22 | Geneohm Sciences Canada Inc. | Compositions and methods for detecting Klebsiella pneumoniae |
| US10047404B2 (en) | 1999-09-28 | 2018-08-14 | Geneohm Sciences Canada, Inc. | Highly conserved tuf genes and their use to generate probes and primers for detection of coagulase-negative Staphylococcus |
Also Published As
| Publication number | Publication date |
|---|---|
| AU7188491A (en) | 1991-08-21 |
| CA2075186A1 (fr) | 1991-08-03 |
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