WO1990011688A1 - Microbes avirulents et leurs emplois - Google Patents
Microbes avirulents et leurs emplois Download PDFInfo
- Publication number
- WO1990011688A1 WO1990011688A1 PCT/US1990/001603 US9001603W WO9011688A1 WO 1990011688 A1 WO1990011688 A1 WO 1990011688A1 US 9001603 W US9001603 W US 9001603W WO 9011688 A1 WO9011688 A1 WO 9011688A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- choleraesuis
- crp
- gene
- avirulent
- strain
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 71
- 241001138501 Salmonella enterica Species 0.000 claims abstract description 64
- 229960005486 vaccine Drugs 0.000 claims abstract description 44
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 42
- 239000000427 antigen Substances 0.000 claims abstract description 37
- 108091007433 antigens Proteins 0.000 claims abstract description 37
- 102000036639 antigens Human genes 0.000 claims abstract description 36
- 244000052769 pathogen Species 0.000 claims abstract description 26
- 101710154303 Cyclic AMP receptor protein Proteins 0.000 claims abstract description 22
- 241000251539 Vertebrata <Metazoa> Species 0.000 claims abstract description 18
- 108060000200 adenylate cyclase Proteins 0.000 claims abstract description 18
- 102000030621 adenylate cyclase Human genes 0.000 claims abstract description 17
- 230000003053 immunization Effects 0.000 claims abstract description 17
- 238000002649 immunization Methods 0.000 claims abstract description 15
- 230000028993 immune response Effects 0.000 claims abstract description 14
- 230000001939 inductive effect Effects 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims description 37
- 230000035772 mutation Effects 0.000 claims description 35
- 101150006779 crp gene Proteins 0.000 claims description 33
- 230000001018 virulence Effects 0.000 claims description 23
- 210000000987 immune system Anatomy 0.000 claims description 19
- 230000002163 immunogen Effects 0.000 claims description 19
- 239000013612 plasmid Substances 0.000 claims description 19
- 230000004936 stimulating effect Effects 0.000 claims description 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 8
- 229920001184 polypeptide Polymers 0.000 claims description 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 230000002759 chromosomal effect Effects 0.000 claims description 4
- 101150084863 cya gene Proteins 0.000 claims description 4
- 239000013598 vector Substances 0.000 claims description 4
- 230000002950 deficient Effects 0.000 claims description 3
- 231100000518 lethal Toxicity 0.000 claims 3
- 230000001665 lethal effect Effects 0.000 claims 3
- 206010061765 Chromosomal mutation Diseases 0.000 claims 1
- 101710088194 Dehydrogenase Proteins 0.000 claims 1
- 238000005215 recombination Methods 0.000 claims 1
- 230000006798 recombination Effects 0.000 claims 1
- 241001465754 Metazoa Species 0.000 description 31
- 210000004027 cell Anatomy 0.000 description 21
- 241000699670 Mus sp. Species 0.000 description 20
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 18
- DGMPVYSXXIOGJY-UHFFFAOYSA-N Fusaric acid Chemical compound CCCCC1=CC=C(C(O)=O)N=C1 DGMPVYSXXIOGJY-UHFFFAOYSA-N 0.000 description 12
- 238000012217 deletion Methods 0.000 description 12
- 230000037430 deletion Effects 0.000 description 12
- 201000010099 disease Diseases 0.000 description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 11
- 108020004414 DNA Proteins 0.000 description 10
- 230000005847 immunogenicity Effects 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 239000013566 allergen Substances 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 210000004837 gut-associated lymphoid tissue Anatomy 0.000 description 7
- 238000010361 transduction Methods 0.000 description 7
- 230000026683 transduction Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 241000282898 Sus scrofa Species 0.000 description 6
- 238000010276 construction Methods 0.000 description 6
- 238000011081 inoculation Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 241000271566 Aves Species 0.000 description 5
- 108020004511 Recombinant DNA Proteins 0.000 description 5
- 241000607142 Salmonella Species 0.000 description 5
- 239000004098 Tetracycline Substances 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 230000000890 antigenic effect Effects 0.000 description 5
- 230000014509 gene expression Effects 0.000 description 5
- 230000036039 immunity Effects 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 229960002180 tetracycline Drugs 0.000 description 5
- 229930101283 tetracycline Natural products 0.000 description 5
- 235000019364 tetracycline Nutrition 0.000 description 5
- 150000003522 tetracyclines Chemical class 0.000 description 5
- IVOMOUWHDPKRLL-KQYNXXCUSA-M 3',5'-cyclic AMP(1-) Chemical compound C([C@H]1O2)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-M 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 210000001035 gastrointestinal tract Anatomy 0.000 description 4
- 229940099472 immunoglobulin a Drugs 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 3
- 206010039438 Salmonella Infections Diseases 0.000 description 3
- 241000282887 Suidae Species 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 230000002238 attenuated effect Effects 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 101150002054 galE gene Proteins 0.000 description 3
- 229930182830 galactose Natural products 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000007918 intramuscular administration Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 206010039447 salmonellosis Diseases 0.000 description 3
- 230000003248 secreting effect Effects 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 230000002463 transducing effect Effects 0.000 description 3
- 241001515965 unidentified phage Species 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 241000938605 Crocodylia Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000237852 Mollusca Species 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- 208000030961 allergic reaction Diseases 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000000680 avirulence Effects 0.000 description 2
- 210000000621 bronchi Anatomy 0.000 description 2
- 108091006374 cAMP receptor proteins Proteins 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 239000013601 cosmid vector Substances 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 238000001990 intravenous administration Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- MHWLWQUZZRMNGJ-UHFFFAOYSA-N nalidixic acid Chemical compound C1=C(C)N=C2N(CC)C=C(C(O)=O)C(=O)C2=C1 MHWLWQUZZRMNGJ-UHFFFAOYSA-N 0.000 description 2
- 229960000210 nalidixic acid Drugs 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 235000015170 shellfish Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000002627 tracheal intubation Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- RNAMYOYQYRYFQY-UHFFFAOYSA-N 2-(4,4-difluoropiperidin-1-yl)-6-methoxy-n-(1-propan-2-ylpiperidin-4-yl)-7-(3-pyrrolidin-1-ylpropoxy)quinazolin-4-amine Chemical compound N1=C(N2CCC(F)(F)CC2)N=C2C=C(OCCCN3CCCC3)C(OC)=CC2=C1NC1CCN(C(C)C)CC1 RNAMYOYQYRYFQY-UHFFFAOYSA-N 0.000 description 1
- 241001167018 Aroa Species 0.000 description 1
- 238000011725 BALB/c mouse Methods 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000251556 Chordata Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 101100491986 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) aromA gene Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010024264 Lethargy Diseases 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 241001625930 Luria Species 0.000 description 1
- 239000006154 MacConkey agar Substances 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 108091061960 Naked DNA Proteins 0.000 description 1
- 229920002274 Nalgene Polymers 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 229940124842 Salmonella vaccine Drugs 0.000 description 1
- 241000701835 Salmonella virus P22 Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 230000024932 T cell mediated immunity Effects 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- HSCJRCZFDFQWRP-ABVWGUQPSA-N UDP-alpha-D-galactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-ABVWGUQPSA-N 0.000 description 1
- HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 230000007503 antigenic stimulation Effects 0.000 description 1
- 101150037081 aroA gene Proteins 0.000 description 1
- -1 aromatic amino acids Chemical class 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000003190 augmentative effect Effects 0.000 description 1
- 230000037429 base substitution Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- DLRVVLDZNNYCBX-ZZFZYMBESA-N beta-melibiose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1 DLRVVLDZNNYCBX-ZZFZYMBESA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000001925 catabolic effect Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 230000007248 cellular mechanism Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 230000016396 cytokine production Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000001400 expression cloning Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 210000003722 extracellular fluid Anatomy 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 101150070420 gyrA gene Proteins 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 230000004727 humoral immunity Effects 0.000 description 1
- 210000003405 ileum Anatomy 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000004957 immunoregulator effect Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 210000001986 peyer's patch Anatomy 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 229960003127 rabies vaccine Drugs 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000014493 regulation of gene expression Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 210000004876 tela submucosa Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000024664 tolerance induction Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/025—Enterobacteriales, e.g. Enterobacter
- A61K39/0275—Salmonella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/42—Salmonella
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- S. choleraesuis strains used in this Example are listed in Table 1. Bacterial strains were grown at 37°C in L broth and on L agar (Lennox, Virology 1: 190-206
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Physics & Mathematics (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Amplifiers (AREA)
- Stereophonic System (AREA)
- Electronic Switches (AREA)
Abstract
L'invention concerne un vaccin d'immunisation d'un vertébré ou d'un invertébré, comprenant un dérivé avirulent de S. choleraesuis. Lesdits dérivés sont incapables de produire une cyclase d'adénylate fonctionnelle et/ou une protéine réceptrice d'AMP cyclique. L'invention concerne également un vaccin d'immunisation d'un vertébré et d'un invertébré, comprenant un dérivé virulent d'un microbe pathogène, ledit dérivé étant incapable de produire une cyclase d'adénylate fonctionnelle et/ ou une protéine réceptrice d'AMP cyclique, tout en étant capable d'exprimer un gène de recombinaison dérivé d'un germe pathogène dudit vertébré, afin de produire un antigène capable de provoquer une réponse immune chez ledit vertébré contre ledit germe pathogène.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US332,285 | 1981-12-18 | ||
US33228589A | 1989-03-31 | 1989-03-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1990011688A1 true WO1990011688A1 (fr) | 1990-10-18 |
Family
ID=23297569
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1990/001603 WO1990011688A1 (fr) | 1989-03-31 | 1990-03-26 | Microbes avirulents et leurs emplois |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0465561A4 (fr) |
JP (1) | JPH04506000A (fr) |
CN (1) | CN1046463A (fr) |
AU (1) | AU5355990A (fr) |
CA (1) | CA2013572A1 (fr) |
WO (1) | WO1990011688A1 (fr) |
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1992019720A1 (fr) * | 1991-05-02 | 1992-11-12 | Institut Pasteur | Souches recombinantes immunogenes de b. anthracis - compositions immunogenes les contenant |
US5840312A (en) * | 1991-05-02 | 1998-11-24 | Institut Pasteur | Recombinant Bacillus anthracis strains unable to produce the lethal factor protein or edema factor protein |
US5922583A (en) * | 1995-10-17 | 1999-07-13 | Biostar Inc. | Methods for production of recombinant plasmids |
US8445254B2 (en) | 2007-05-10 | 2013-05-21 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Regulated expression of antigen and/or regulated attentuation to enhance vaccine immunogenicity and/or safety |
US8465755B2 (en) | 2007-10-05 | 2013-06-18 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium capable of eliciting an immune response against enteric pathogens |
US8889121B2 (en) | 2010-01-22 | 2014-11-18 | The Arizona Board Of Regents For An On Behalf Of Arizona State University | Bacterium comprising a regulated rfaH nucleic acid |
US9045742B2 (en) | 2009-05-29 | 2015-06-02 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant Edwardsiella bacterium |
US9050285B2 (en) | 2008-10-17 | 2015-06-09 | The United States of America National Institutes of Health (NH), U.S. Dept. of Health and Human Services (DHHS) | Recombinant bacterium capable of eliciting an immune response against Streptococcus pneumoniae |
US9062297B2 (en) | 2010-01-13 | 2015-06-23 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Yersinia pestis vaccine |
US9163219B2 (en) | 2009-04-14 | 2015-10-20 | Arizona Board Of Regents On Behalf Of Arizona State University | Single expression vector for generation of a virus with a segmented genome |
US9303264B2 (en) | 2012-05-18 | 2016-04-05 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Photosynthetic microorganisms expressing thermostable lipase |
US9481888B2 (en) | 2009-05-22 | 2016-11-01 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium and methods of antigen and nucleic acid delivery |
US9580718B2 (en) | 2013-06-17 | 2017-02-28 | Arizona Board Of Regents On Behalf Of Arizona State University | Attenuated live bacteria with increased acid resistance and methods of use thereof |
US9598697B2 (en) | 2010-05-28 | 2017-03-21 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium to decrease tumor growth |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE234917T1 (de) * | 1990-11-09 | 2003-04-15 | Univ Washington | Avirulente salmonella mikroben mit einer mutation in dem cdt gen und ihre verwendung |
US6713073B1 (en) * | 1998-07-24 | 2004-03-30 | Megan Health, Inc. | Method of vaccination of newly hatched poultry |
-
1990
- 1990-03-26 AU AU53559/90A patent/AU5355990A/en not_active Abandoned
- 1990-03-26 JP JP2505540A patent/JPH04506000A/ja active Pending
- 1990-03-26 EP EP19900905860 patent/EP0465561A4/en not_active Withdrawn
- 1990-03-26 WO PCT/US1990/001603 patent/WO1990011688A1/fr not_active Application Discontinuation
- 1990-03-30 CA CA002013572A patent/CA2013572A1/fr not_active Abandoned
- 1990-03-31 CN CN90102664A patent/CN1046463A/zh active Pending
Non-Patent Citations (6)
Title |
---|
Immunological Investigations, Volume 18, pages 583-596. April 1989. R. CURTISS et al. Recombinant Avirulent Salmonella Vaccine Strains with stable maintenance and high level expression of cloned genes in vivo. See Abstract. * |
Infection and Immunity, Volume 55(12), pages 3035-3043. R. CURTISS et al. December 1987. Salmonella typhimurium Deletion Mutants Lacking Adenylate Cyclase and Cyclic AMP Receptor Protein are Avirulent and Immunogenic. See Abstract. * |
Infection and Immunity, Volume 55(4), pages 955-962. N.A. NNALUE et al. April 1987. "Tests of the Virulence and Live-Vaccine Efficacy of Auxotrophic and gal E Derivatives of Salmonella choleraesuis. See Abstract. * |
Japan Journal of Veternary Science, Volume 50, pages 706-713. July 1988. N'AKAMURA, et al. Virulence and Immunogenicity of Plasimid-cured Salmonella serovar Enteritidis AL1192 against Cattle. See Abstract. * |
Microbial Pathogenesis, Volume 7, pages 299-310. December 1989. N.A. NNALUE et al. Vaccination Route, infectivity and thioglycollate broth administration: effects on live vaccine efficacy of auxotrophic derivatives of Salmonella Choleraesuis. See Abstract. * |
See also references of EP0465561A4 * |
Cited By (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5840312A (en) * | 1991-05-02 | 1998-11-24 | Institut Pasteur | Recombinant Bacillus anthracis strains unable to produce the lethal factor protein or edema factor protein |
WO1992019720A1 (fr) * | 1991-05-02 | 1992-11-12 | Institut Pasteur | Souches recombinantes immunogenes de b. anthracis - compositions immunogenes les contenant |
US5922583A (en) * | 1995-10-17 | 1999-07-13 | Biostar Inc. | Methods for production of recombinant plasmids |
US9297015B2 (en) | 2007-05-10 | 2016-03-29 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Regulated expression of antigen and/or regulated attentuation to enhance vaccine immunogenicity and/or safety |
US8445254B2 (en) | 2007-05-10 | 2013-05-21 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Regulated expression of antigen and/or regulated attentuation to enhance vaccine immunogenicity and/or safety |
US11920139B2 (en) | 2007-05-10 | 2024-03-05 | The Washington University | Regulated expression of antigen and/or regulated attenuation to enhance vaccine immunogenicity and/or safety |
US11180765B2 (en) | 2007-05-10 | 2021-11-23 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Regulated expression of antigen and/or regulated attenuation to enhance vaccine immunogenicity and/or safety |
US10774334B2 (en) | 2007-05-10 | 2020-09-15 | Arizona Broad of Regents on Behalf of Arizona State University | Regulated expression of antigen and/or regulated attenuation to enhance vaccine immunogenicity and/or safety |
US8465755B2 (en) | 2007-10-05 | 2013-06-18 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium capable of eliciting an immune response against enteric pathogens |
US9040059B2 (en) | 2007-10-05 | 2015-05-26 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium capable of eliciting a protective immune response against C. perfringens |
US9050285B2 (en) | 2008-10-17 | 2015-06-09 | The United States of America National Institutes of Health (NH), U.S. Dept. of Health and Human Services (DHHS) | Recombinant bacterium capable of eliciting an immune response against Streptococcus pneumoniae |
US9163219B2 (en) | 2009-04-14 | 2015-10-20 | Arizona Board Of Regents On Behalf Of Arizona State University | Single expression vector for generation of a virus with a segmented genome |
US9481888B2 (en) | 2009-05-22 | 2016-11-01 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium and methods of antigen and nucleic acid delivery |
US9045742B2 (en) | 2009-05-29 | 2015-06-02 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant Edwardsiella bacterium |
US9062297B2 (en) | 2010-01-13 | 2015-06-23 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Yersinia pestis vaccine |
US8889121B2 (en) | 2010-01-22 | 2014-11-18 | The Arizona Board Of Regents For An On Behalf Of Arizona State University | Bacterium comprising a regulated rfaH nucleic acid |
US9598697B2 (en) | 2010-05-28 | 2017-03-21 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Recombinant bacterium to decrease tumor growth |
US9303264B2 (en) | 2012-05-18 | 2016-04-05 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Photosynthetic microorganisms expressing thermostable lipase |
US9580718B2 (en) | 2013-06-17 | 2017-02-28 | Arizona Board Of Regents On Behalf Of Arizona State University | Attenuated live bacteria with increased acid resistance and methods of use thereof |
Also Published As
Publication number | Publication date |
---|---|
EP0465561A1 (fr) | 1992-01-15 |
CN1046463A (zh) | 1990-10-31 |
AU5355990A (en) | 1990-11-05 |
EP0465561A4 (en) | 1992-04-08 |
CA2013572A1 (fr) | 1990-09-30 |
JPH04506000A (ja) | 1992-10-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5468485A (en) | Avirulent microbes and uses therefor | |
AU623599B2 (en) | Avirulent microbes, incapable of producing functional adenylate cyclase and cyclic amp | |
US5294441A (en) | Avirulent microbes and uses therefor: salmonella typhi | |
US5387744A (en) | Avirulent microbes and uses therefor: Salmonella typhi | |
AU645489B2 (en) | Vaccines containing avirulent phoP-type microorganisms | |
US4735801A (en) | Novel non-reverting salmonella live vaccines | |
US6399074B1 (en) | Live attenuated salmonella vaccines to control avian pathogens | |
AU640344B2 (en) | Novel non-reverting shigella flexneri linve vaccines | |
US4550081A (en) | Non-reverting salmonella | |
US4837151A (en) | Live vaccines comprising two mutations and foreign antigen | |
US5855880A (en) | Avirulent microbes and uses therefor | |
Curtiss III et al. | Nonrecombinant and recombinant avirulent Salmonella live vaccines for poultry | |
EP0500699B1 (fr) | Vaccins de protection croisee contre la salmonella | |
CA2244807A1 (fr) | Vaccin produit a partir de salmonella typhimurium | |
JPH11235191A (ja) | 細胞の遺伝的集団において所望の組換え遺伝子を維持する方法 | |
WO1990011688A1 (fr) | Microbes avirulents et leurs emplois | |
AU666108B2 (en) | CDT mutants of salmonella typhi | |
IE84199B1 (en) | Avirulent salmonella microbes comprising a mutation in the CDT gene and uses therefor |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AU JP |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB IT LU NL SE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1990905860 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 1990905860 Country of ref document: EP |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1990905860 Country of ref document: EP |