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WO1990007259A3 - Procede et appareil de triage de cellules ou de corps formes a l'aide de populations exprimant des caracteristiques selectionnees - Google Patents

Procede et appareil de triage de cellules ou de corps formes a l'aide de populations exprimant des caracteristiques selectionnees Download PDF

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Publication number
WO1990007259A3
WO1990007259A3 PCT/US1989/005548 US8905548W WO9007259A3 WO 1990007259 A3 WO1990007259 A3 WO 1990007259A3 US 8905548 W US8905548 W US 8905548W WO 9007259 A3 WO9007259 A3 WO 9007259A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
microspheres
cell population
blood cell
formed bodies
Prior art date
Application number
PCT/US1989/005548
Other languages
English (en)
Other versions
WO1990007259A2 (fr
Inventor
Kenneth H Kortright
Wallace H Coulter
Carlos Rodriguez
Thomas Russell
Ronald Paul
Original Assignee
Coulter Electronics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coulter Electronics filed Critical Coulter Electronics
Priority to JP2501656A priority Critical patent/JP2795746B2/ja
Priority to AU48218/90A priority patent/AU655154B2/en
Priority to BR898907826A priority patent/BR8907826A/pt
Priority to EP90901352A priority patent/EP0472522B1/fr
Priority to DE68928085T priority patent/DE68928085T2/de
Publication of WO1990007259A2 publication Critical patent/WO1990007259A2/fr
Publication of WO1990007259A3 publication Critical patent/WO1990007259A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1024—Counting particles by non-optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1028—Sorting particles

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Dispersion Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé et un appareil (320, 340, 360) permettant la récupération, le comptage et/ou l'analyse automatique et rapide d'au moins une population sélectionnée de cellules ou de corps formés, tels qu'une population de globules blancs ainsi que d'au moins un sous ensemble de cette dernière d'un échantillon de sang entier (322, 342) ou d'une partie de celui-ci. On prépare un volume d'un milieu biologique contenant les globules blancs, on y introduit au moins un réactif (326, 346) spécifique ou préférentiel au moins à plusieurs cellules biologiques sélectionnées, puis on les mélange (384) pendant une courte période de temps. On peut modifier le paramètre d'opacité et/ou de volume des cellules, puis on compte et on analyse (332, 352, 416, 422) le mélange en une ou plusieurs étapes afin d'obtenir l'analyse de la population et du sous-ensemble de globules blancs. L'échantillon biologique peut être un échantillon de sang entier et le réactif peut inclure ou être un lysat ou un anticorps monoclonal lié à des microsphères, se liant à des cellules spécifiques ou à une combinaison de lysat et de microsphères auxquelles des anticorps sont liés. Les microsphères peuvent être magnétiques et les cellules liées peuvent être magnétiquement retirées afin de récupérer et d'analyser la population de cellules sanguines restante.
PCT/US1989/005548 1987-03-13 1989-12-13 Procede et appareil de triage de cellules ou de corps formes a l'aide de populations exprimant des caracteristiques selectionnees WO1990007259A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2501656A JP2795746B2 (ja) 1988-12-16 1989-12-13 選択された特性を現わす集団についての細胞または形成体をスクリーニングする方法および装置
AU48218/90A AU655154B2 (en) 1987-03-13 1989-12-13 Method and apparatus for screening cells or formed bodies with populations expressing selected characteristics
BR898907826A BR8907826A (pt) 1988-12-16 1989-12-13 Processo e aparelho para obter pelo menos uma analise de populacao de celulas sanguineas brancas,processo e aparelho para obter uma populacao de celulas sanguineas brancas,de partes multiplas,processo e aparelho para obter uma diferencial de populacao de celulas sanguineas brancas de multiplas partes e processo e aparelho para obter uma classificacao de uma amostra de celulas ou corpos formados
EP90901352A EP0472522B1 (fr) 1988-12-16 1989-12-13 Procede de triage de cellules ou de corps formes a l'aide de populations exprimant des caracteristiques selectionnees
DE68928085T DE68928085T2 (de) 1988-12-16 1989-12-13 Verfahren zur auslese bestimmter zellen oder zellgebilden anhand von populationen mit ausgewählten merkmalen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28585688A 1988-12-16 1988-12-16
US285,856 1988-12-16

Publications (2)

Publication Number Publication Date
WO1990007259A2 WO1990007259A2 (fr) 1990-07-12
WO1990007259A3 true WO1990007259A3 (fr) 1990-11-01

Family

ID=23095999

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1989/005548 WO1990007259A2 (fr) 1987-03-13 1989-12-13 Procede et appareil de triage de cellules ou de corps formes a l'aide de populations exprimant des caracteristiques selectionnees

Country Status (9)

Country Link
EP (1) EP0472522B1 (fr)
JP (1) JP2795746B2 (fr)
CN (1) CN1034242C (fr)
AT (1) ATE153758T1 (fr)
AU (1) AU655154B2 (fr)
BR (1) BR8907826A (fr)
CA (1) CA1339840C (fr)
DE (1) DE68928085T2 (fr)
WO (1) WO1990007259A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5385822A (en) * 1988-05-02 1995-01-31 Zynaxis, Inc. Methods for detection and quantification of cell subsets within subpopulations of a mixed cell population
CA1333047C (fr) * 1989-04-14 1994-11-15 Thomas Russell Methode et appareil pour obtenir au moins une analyse de la population de globules blancs
EP0558639A1 (fr) * 1990-11-23 1993-09-08 Coulter Corporation Procede et appareil de triage optique de cellules microscopiques
US5374396A (en) * 1992-05-05 1994-12-20 Tsi Incorporated Syringe injection system for measuring non-volatile residue in solvents
US5351523A (en) * 1993-01-21 1994-10-04 Tsi Incorporated Apparatus and process for determining filter efficiency in removing colloidal suspensions
US20090304659A1 (en) * 2008-06-06 2009-12-10 Baylor Research Institute Anti-cd8 antibodies block priming of cytotoxic effectors and lead to generation of regulatory cd8+ t cells
EP2259044A1 (fr) * 2009-06-05 2010-12-08 Koninklijke Philips Electronics N.V. Procédé et dispositif d'impédance multi-fréquence pour la discrimination et le comptage de particules exprimant un marqueur spécifique
EP2259045A1 (fr) * 2009-06-05 2010-12-08 Koninklijke Philips Electronics N.V. Procédé et dispositif d'impédance multi-fréquence pour la discrimination et le comptage de particules exprimant un marqueur spécifique

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3925018A (en) * 1973-06-08 1975-12-09 Technicon Instr Method and apparatus for quantitative analysis utilizing particulate reagent material
US3970518A (en) * 1975-07-01 1976-07-20 General Electric Company Magnetic separation of biological particles
US4141687A (en) * 1976-03-12 1979-02-27 Technicon Instruments Corporation Automatic apparatus and method for the assay of fluid samples
US4219335A (en) * 1978-09-18 1980-08-26 E. I. Du Pont De Nemours And Company Immunochemical testing using tagged reagents
US4219411A (en) * 1978-09-18 1980-08-26 California Institute Of Technology Cell sorting apparatus
US4284412A (en) * 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4452773A (en) * 1982-04-05 1984-06-05 Canadian Patents And Development Limited Magnetic iron-dextran microspheres
US4499052A (en) * 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
US4599307A (en) * 1983-07-18 1986-07-08 Becton, Dickinson And Company Method for elimination of selected cell populations in analytic cytology
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US4747685A (en) * 1985-03-29 1988-05-31 Kabushiki Kaisha Toshiba Biological microparticle inspection apparatus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4206094A (en) * 1976-06-09 1980-06-03 California Institute Of Technology Method for producing a biological reagent
CA1311404C (fr) * 1987-03-13 1992-12-15 Kenneth H. Kortright Analyseur automatique et methode pour le depistage de cellules ou de corps formes, servant a compter les populations presentant des caracteristiques selectionnees

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3925018A (en) * 1973-06-08 1975-12-09 Technicon Instr Method and apparatus for quantitative analysis utilizing particulate reagent material
US3970518A (en) * 1975-07-01 1976-07-20 General Electric Company Magnetic separation of biological particles
US4141687A (en) * 1976-03-12 1979-02-27 Technicon Instruments Corporation Automatic apparatus and method for the assay of fluid samples
US4219335A (en) * 1978-09-18 1980-08-26 E. I. Du Pont De Nemours And Company Immunochemical testing using tagged reagents
US4219411A (en) * 1978-09-18 1980-08-26 California Institute Of Technology Cell sorting apparatus
US4284412A (en) * 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4452773A (en) * 1982-04-05 1984-06-05 Canadian Patents And Development Limited Magnetic iron-dextran microspheres
US4499052A (en) * 1982-08-30 1985-02-12 Becton, Dickinson And Company Apparatus for distinguishing multiple subpopulations of cells
US4599307A (en) * 1983-07-18 1986-07-08 Becton, Dickinson And Company Method for elimination of selected cell populations in analytic cytology
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US4747685A (en) * 1985-03-29 1988-05-31 Kabushiki Kaisha Toshiba Biological microparticle inspection apparatus

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Clinical Chemistry, Vol. 23, No. 8, issued 1977 LEIF, et al. "Development of Instrumentation and Fluorochromes for Automated Multiparameter Analysis of Cells", pages 1492-1498. See full document, especially p. 148. *
Clinical Instrument Systems, Vol. 7, No. 5, issued May 1986, "CBC/DIFF Automation" Macor Publishing company, pp. 1-8. *
Federation of Europan Biochemical Societies (FEBS) Vol. 170, No. 2, issued May 1984, "Separation of cells...magnetic, chromatography" MOLDAY, et al. pp. 232-237, see discussion, p. 237. "Separation of cells labeled with immunospecific iron dextran microspheres...". *
Journal of Immunological Methods, Vol. 52 MOLDAY, et al. issued 1982 "Immunospecific Ferromagnetic Iron-Dextran reagents for the labeling and magnetic separation of cells pp. 353-367, see pp. 355-357. *

Also Published As

Publication number Publication date
CA1339840C (fr) 1998-04-28
EP0472522A1 (fr) 1992-03-04
WO1990007259A2 (fr) 1990-07-12
ATE153758T1 (de) 1997-06-15
AU655154B2 (en) 1994-12-08
JPH04503405A (ja) 1992-06-18
EP0472522A4 (en) 1992-08-05
CN1034242C (zh) 1997-03-12
DE68928085D1 (de) 1997-07-03
BR8907826A (pt) 1991-10-22
DE68928085T2 (de) 1998-01-15
JP2795746B2 (ja) 1998-09-10
CN1043566A (zh) 1990-07-04
AU4821890A (en) 1990-08-01
EP0472522B1 (fr) 1997-05-28

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