WO1989000375A1 - Souches de trichoderma competentes dans la rhizosphere - Google Patents
Souches de trichoderma competentes dans la rhizosphere Download PDFInfo
- Publication number
- WO1989000375A1 WO1989000375A1 PCT/US1988/002011 US8802011W WO8900375A1 WO 1989000375 A1 WO1989000375 A1 WO 1989000375A1 US 8802011 W US8802011 W US 8802011W WO 8900375 A1 WO8900375 A1 WO 8900375A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- rhizosphere
- trichoderma
- atcc
- group
- spp
- Prior art date
Links
- 241000223259 Trichoderma Species 0.000 title claims description 35
- 239000012681 biocontrol agent Substances 0.000 claims abstract description 15
- 241000223261 Trichoderma viride Species 0.000 claims abstract description 10
- 241000233866 Fungi Species 0.000 claims abstract description 8
- 241000123975 Trichoderma polysporum Species 0.000 claims abstract description 7
- 201000010099 disease Diseases 0.000 claims abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 7
- 241000813090 Rhizoctonia solani Species 0.000 claims abstract description 6
- 241001558929 Sclerotium <basidiomycota> Species 0.000 claims abstract description 3
- 241000593874 Chondrostereum purpureum Species 0.000 claims abstract 5
- 241000233639 Pythium Species 0.000 claims abstract 3
- 241000196324 Embryophyta Species 0.000 claims description 13
- RIOXQFHNBCKOKP-UHFFFAOYSA-N benomyl Chemical compound C1=CC=C2N(C(=O)NCCCC)C(NC(=O)OC)=NC2=C1 RIOXQFHNBCKOKP-UHFFFAOYSA-N 0.000 claims description 12
- MITFXPHMIHQXPI-UHFFFAOYSA-N benzoxaprofen Natural products N=1C2=CC(C(C(O)=O)C)=CC=C2OC=1C1=CC=C(Cl)C=C1 MITFXPHMIHQXPI-UHFFFAOYSA-N 0.000 claims description 12
- 240000008067 Cucumis sativus Species 0.000 claims description 9
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 7
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 6
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 240000006497 Dianthus caryophyllus Species 0.000 claims description 5
- 235000009355 Dianthus caryophyllus Nutrition 0.000 claims description 5
- 240000005979 Hordeum vulgare Species 0.000 claims description 5
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 5
- 240000008415 Lactuca sativa Species 0.000 claims description 5
- 235000003228 Lactuca sativa Nutrition 0.000 claims description 5
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 5
- 244000088415 Raphanus sativus Species 0.000 claims description 5
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 5
- 240000003768 Solanum lycopersicum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 5
- 235000009973 maize Nutrition 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims 4
- 239000008365 aqueous carrier Substances 0.000 claims 4
- 241000006351 Leucophyllum frutescens Species 0.000 claims 3
- 241000135371 Sclerotium sp. (in: Ascomycota) Species 0.000 claims 3
- 241000221198 Basidiomycota Species 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 25
- 239000001913 cellulose Substances 0.000 description 11
- 229920002678 cellulose Polymers 0.000 description 11
- 239000000758 substrate Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 238000003556 assay Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241001646398 Pseudomonas chlororaphis Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000002538 fungal effect Effects 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000001332 colony forming effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 210000000416 exudates and transudate Anatomy 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000003471 mutagenic agent Substances 0.000 description 2
- 231100000707 mutagenic chemical Toxicity 0.000 description 2
- 230000008659 phytopathology Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000006152 selective media Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004366 Glucosidases Human genes 0.000 description 1
- 108010056771 Glucosidases Proteins 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- -1 Polypropylene Polymers 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000918585 Pythium aphanidermatum Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000003816 axenic effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 229940105329 carboxymethylcellulose Drugs 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 244000000034 soilborne pathogen Species 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
Definitions
- This invention generally relates to new biotypes of soilborne biocontrol agents. More specifically it relates to rhizosphere-competent biocontrol agents.
- rhizosphere competence has been employed to describe an attribute of rhizobia characterized by their consistent association with legume root nodules.
- rhizosphere-competence is used to describe the ability of a microorganism to grow and function in the developing rhizosphere. In application, this means that the microorganism can be placed on the seed, it can grow and it can colonize the rhizosphere of the developing root.
- Trichoderma spp. such as Trichoderma harzanium Rifai act as biological control agents against certain plant diseases. Nonetheless, use of Trichoderma spp., as biocontrol agents has been rather limited. This is mainly because seed treatment with Trichoderma spp. generally does not provide continued protection for the emerging root system of the maturing plant. Such seed treatment does serve to reduce preemergence damping-off, but the root system is left unprotected. It is generally believed that this failure to protect the root system is because Trichoderma spp.
- Trichoderma species are Trichoderma polvsporua, ATCC number 20852 and Trichoderma viride, ATCC number 20853. They are particularly useful in controlling diseases caused by Phythium spp. , Sclerotium spp. , and Rhizoctonia solani in such varied plants as beans, maize, tomato, radish, cucumber, wheat, barley, lettuce and carnations. Applicants also have found that N- methyl-N-nitro-N-nitrosoguanidine was a particularly useful mutagenic chemical in producing Trichoderma species which possess the desired rhizosphere- competence characteristic. Other useful mutagenic agents would include, but not limited to ultraviolet light and x-ray radiation.
- Trichoderma e.g., the Trichoderma polysporum and Trichoderma viride species disclosed herein
- Trichoderma are particularly effective when they are mutated for both rhizosphere- competence and benomyl tolerance.
- the herein disclosed strains are grown in a nutrient medium where cellulose represents a major part of the microorganisms source of carbon, or more preferably its only source of carbon. Representative sources of carbon would include, but not be limited to carboxy-methyl-cellulose, cotton linters, solka floe, and Sigma Cell 20 (Sigma Chemicals, St. Louis) .
- Figure 1 is a bar graph depicting the dry weight of mycelium of strains of two Trichoderma spp. grown for 6 days in Czapek Dox broth with 37. Cellulose as the sole source of carbon.
- Figures 2A and 2B depict the population densities of Trichoderma spp. in the rhizosphere soil of cucumber plants without benomyl added to the soil.
- Figure 2 shows the result for T_j_ polysporum ATCC 20475 and ATCC 20853.
- Figure 3 is a bar graph depicting the rhizosphere competence (RC) index of T_j_ polvsporum T.P. 18, T_i_ viride ATCC 20476, and Tj. viride T.V. 62.
- RC rhizosphere competence
- Rhizosphere Competence Various methods have been employed to test rhizosphere competence. These were primarily based on a comparison of the number of colony forming units ("cfu") of microorganisms in the soil associated with roots when compared to population densities in non-rhizosphere soil.
- the rhizosphere competence assay used in this research effort was also directed toward improving measurement in time and space of the activity of potential rhizosphere inhabitants. Certain criteria were demanded by the experimental questions to be examined. Quantitative analysis of population densities at each depth of root was also necessary. No water was added during incubation obviating the possibility of propagules being washed into the rhizosphere.
- the rhizosphere competence assay provides the best bioassay yet developed for the rhizosphere nitrous statues at root tips. It has potential for use in a wide variety of experimental problems related to ecological and nutritional interactions in the rhizosphere.
- Trichoderma spp. disclosed herein were tested for rhizosphere competence by coating seeds with the respective isolates and following population densities of the fungus to a root depth of 8 cm. Both grew to a depth of 8 cm. Again Trichoderma spp. which are benomyl tolerant as well as rhizosphere competent are preferred embodiments of this invention.
- Our particular rhizosphere- competence assays were conducted as follows. Polypropylene centrifuge tubes (28.6 by 103.6 mm) were sliced longitudinally into two halves. Each half was filled with moistened soil (-3 bars) and pre-incubated for 48 hours in plastic bags. One treated seed was placed on the half-tube 1 cm below the rim.
- the unseeded half-tube was placed on the first half and secured with rubber bands. Tubes were completely randomized and lots in portions of six each were placed vertically in 10 cm diameter plastic pots. Soil, previously moistened to -0.03 bars and of the same pH as in the tubes, was added to the pots so that the length of the tube was surrounded by the soil, with the top 1 cm of each tube being uncovered. No water was added to the tubes or the pots after seed were sown. Pots were covered with plastic bags to maintain constant metric potential leaving enough space above the tubes for the plants to grow. Pots were placed under constant illumination supplied by 10 white, 40-watt, 120 cm long fluorescent lamps (approximately 5000 lux) , at desired temperatures.
- MATERIALS Soil Nunn sandy loam was used in these investigations. Water content of 43.2 kg portions was adjusted to -0.03 bars and the soil was stored for 48 hours before use. The soils had the following characteristics: pH 7.0, conductivity 0.4 mmhos, lime low, organic matter 1.4%, No 3 -N 1 hg/g, P 9 hg/g. K 198 hg/g, Zn 0.5 hg/g, Fe 3.2 hg/g. Its pH was measured- by the CaCl 2 method.
- Trichoderma polvsporum ATCC 20475 and Trichoderma viride ATCC 20476 were exposed to 100 mg/ml of N- methyl-N-nitro-N-nitrosoguanidine (Sigma Chemicals Co., St. Louis, Mo.) for 30 minutes. The conidia were centrifuged at 2500 g for 15 minutes and resuspended in sterile water three times. Seeds of cucumber (Cucumis sativus L. "straight Eight") , were surface disinfected for 10 minutes in 1.1% sodium hypochlorite solution and 5% ethanol, washed in distilled water, and air dried. Seeds were treated with conidial suspensions of each of the two Trichoderma spp. in water containing 2% (v/w) Pelgel (The Nitragen Co., Milwaukee, WI) as spreader or sticker. Conidial density was adjusted to 10 6 per seed. Controls were treated with Pelgel alone.
- Trichoderma spp. in liquid culture. Strains of each of the two subject Trichoderma spp. were grown in 250 ml Erlenmeyer flasks containing 50 ml Czapek Dox broth on a rotary shaker at 100 rpm at 26°C for 6 days. Cellulose (Sigma Cell 20) was used as sole source of carbon. Each flask was seeded with a 4 mm diameter disk of PDA on which the strains had been grown for 2 days. After 6 days the hyphal mat was removed aseptically and dried for 2 days at 60°C to obtain the weight of mycelium. There were six replicates per strain. Rhizosphere competence (RC) index.
- RC Rhizosphere competence
- T ⁇ polysporum ATCC 20853
- T_j_ viride ATCC 20476
- ⁇ . polvsporum ATCC 20475
- T ⁇ . viride ATCC 20476
- the mutation of these species by the mutagen, mentioned above, and selection on Trichoderma-selective medium has resulted in T ⁇ polysporum (ATCC 20852) and ⁇ viride (ATCC 20853) tolerant to at least 10 mg/ l a.i. benomyl.
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Soil Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Le Trichoderma polysporum (ATCC 20852) compétent dans la rhizosphère et le Trichoderma viride (ATCC 20853) compétent dans la rhizosphère sont décrits. Ce sont respectivement, des mutants du Trichoderma polysporum ATCC 20475 incompétent dans la rhizosphère et du Trichoderma viride ATCC 20476 incompétent dans la rhizosphère. Les souches compétentes dans la rhizosphère sont des agents biorégulateurs particulièrement efficaces pour combattre les maladies des plantes et/ou des arbres associées à divers champignons p.ex., Pythium spp., Sclerotium, spp., Rhizoctonia solani et des basidiomycètes tels que le Chondrostereum purpureum.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7234787A | 1987-07-10 | 1987-07-10 | |
| US072,347 | 1987-07-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1989000375A1 true WO1989000375A1 (fr) | 1989-01-26 |
Family
ID=22107016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1988/002011 WO1989000375A1 (fr) | 1987-07-10 | 1988-06-13 | Souches de trichoderma competentes dans la rhizosphere |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO1989000375A1 (fr) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990003732A1 (fr) * | 1988-10-04 | 1990-04-19 | Novo Nordisk A/S | Preparations a action fongicide |
| WO1990013219A1 (fr) * | 1989-05-05 | 1990-11-15 | Allelix Crop Technologies | Amelioration de la croissance de plants de coniferes |
| US8716001B2 (en) | 2009-02-06 | 2014-05-06 | Cornell University | Trichoderma strains that induce resistance to plant diseases and/or increase plant growth |
-
1988
- 1988-06-13 WO PCT/US1988/002011 patent/WO1989000375A1/fr unknown
Non-Patent Citations (12)
| Title |
|---|
| PHYTOPATHOLOGY, Volume 69, issued May 1979, (Saint Paul, Minnesota, USA), LOCKE et al., "Biological control of Pythium seed rot of peas with Trichoderma Conidia", page 536. * |
| PHYTOPATHOLOGY, Volume 71, issued January 1981, (Saint Paul, Minnesota, USA), G. PAPAVIZAS, "Survival of Trichoderma Harzianum in soil and in pea and bean Rhizospheres", pages 121-125, see page 123 in particular. * |
| PHYTOPATHOLOGY, Volume 72, issued January 1982, (Saint Paul, Minnesota, USA), PAPAVIZAS et al., "Evaluation of new biotypes of Trichoderma Harzianum for tolerance to Benomyl and enhanced biocontrol capabilities", pages 126-132, see pages 130 and 131 in particular. * |
| PHYTOPATHOLOGY, Volume 75, issued November 1985, (Saint Paul, Minnesota, USA), AHMAD et al., "Induction of rhizosphere competence in Trichoderma Harzianum", page 1302. * |
| PHYTOPATHOLOGY, Volume 76, issued May 1986, (Saint Paul, Minnesota, USA), CHAO et al., "Colonization of the rhizosphere by biological control agents applied to seeds", pages 60-65, see pages 64 and 65 in particular. * |
| PHYTOPATHOLOGY, Volume 77, issued February 1987, (Saint Paul, Minnesota, USA), AHMAD et al., "Competitive saprophytic ability and cellulolytic activity of rhizosphere-competent mutants of Trichoderma harzianum", pages 358-362, see pages 361 and 362 in particular. * |
| PHYTOPATHOLOGY, Volume 77, issued February 1987, (Saint Paul, Minnesota, USA), AHMAD et al., "Rhizosphere competence of Trichoderma harzianum", pages 182-189, see pages 188 in particular. * |
| PLANT DISEASE, Volume 69, issued May 1985, (Saint Paul, Minnesota, USA), LIFSHITZ et al., "Decrease in incidence of Rhizoctonia preemergence damping-off by use of integrated chemical and biological controls", pages 431-434, see page 432 in particular. * |
| PLANT DISEASE, Volume 70, issued February 1986, (Saint Paul, Minnesota, USA), CHANG et al., "Increased growth of plants in the presence of the biological control agent Trichoderma Harzianum", pages 145-148, see pages 147 in particular. * |
| PROCEEDINGS OF THE INDIAN NATIONAL SCIENCE ACADEMY PART B, Volume 41, issued November 1975, (Bangladore, India), S. MALL, "Rhizoctonia diseases of legume crops as affected by Trichoderma Viride", pages 559-563, see page 561 in particular. * |
| R. COOK, "Ultrastructure of the root-soil interface", published 1983, by FOSTER et al., (Saint Paul, Minnesota, USA), page v. * |
| S. GARRETT, "Pathogenic root-infecting fungi", published 1970, by S. GARRETT, (Cambridge, England), page 115. * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990003732A1 (fr) * | 1988-10-04 | 1990-04-19 | Novo Nordisk A/S | Preparations a action fongicide |
| WO1990013219A1 (fr) * | 1989-05-05 | 1990-11-15 | Allelix Crop Technologies | Amelioration de la croissance de plants de coniferes |
| US8716001B2 (en) | 2009-02-06 | 2014-05-06 | Cornell University | Trichoderma strains that induce resistance to plant diseases and/or increase plant growth |
| US8877481B2 (en) | 2009-02-06 | 2014-11-04 | Cornell University | Trichoderma strains that induce resistance to plant diseases and/or increase plant growth |
| US8877480B2 (en) | 2009-02-06 | 2014-11-04 | Cornell University | Trichoderma strains that induce resistance to plant diseases and/or increase plant growth |
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