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WO1988009819A3 - Passage biosynthetique pour threonine de c. glutamicum - Google Patents

Passage biosynthetique pour threonine de c. glutamicum Download PDF

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Publication number
WO1988009819A3
WO1988009819A3 PCT/US1988/002029 US8802029W WO8809819A3 WO 1988009819 A3 WO1988009819 A3 WO 1988009819A3 US 8802029 W US8802029 W US 8802029W WO 8809819 A3 WO8809819 A3 WO 8809819A3
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WO
WIPO (PCT)
Prior art keywords
glutamicum
thrc
hom
thrb
sequences
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PCT/US1988/002029
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English (en)
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WO1988009819A2 (fr
Inventor
Anthony J Sinskey
Maximillian T Follettie
Wolfgang Liebl
Oliver P Peoples
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Massachusetts Inst Technology
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Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology
Publication of WO1988009819A2 publication Critical patent/WO1988009819A2/fr
Publication of WO1988009819A3 publication Critical patent/WO1988009819A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Procédé permettant l'isolement et la caractérisation de gènes de C. glutamicum mis en oeuvre dans la biosynthèse d'acides aminés spécifiquement codant hom, thrB et thrC et de séquences régulant leur expression. Sont également décrites des techniques permettant de modifier ou de remplacer ces séquences ainsi qu'un moyen facilitant davantage les isolations et caractérisations, comprenant des vecteurs de sonde activateurs, utiles dans la sélection d'activateurs et de répresseurs hautement efficaces, et pouvant être régulés. On a constitué une nomenclature génomique de C. glutamicum par clivage d'ADN chromosomique avec des enzymes de restriction, par introduction de fragments d'ADN dans un vecteur approprié et par transport des molécules (rDNA) recombinantes ainsi obtenues en C. glutamicum. On a isolé des gènes hom, thrB et thrC biosynthétiques d'acides aminés codant respectivement la déshydrogénase d'homosérine, la kinase d'homosérine et la synthétase de thréonine, par moyen complémentaire d'auxotrophes de C. glutamicum. On a sous-cloné des gènes hom-thrB sur un fragment chromosomique produit par 3,6 kb Sal1 tandis que l'activité thrC avait été isolée d'un second plasmide recombinant au sein de la nomenclature génomique, et soucloné sur un fragment produit par 2.7 kb Sph1. Les places des hom-thrB et du thrC, ainsi que les séquences régulatrices ont été définies par analyses d'enzymes, moyen complémentaire d'auxotrophes E. coli définies, et cartographie de nucléase S1 et de délétion.
PCT/US1988/002029 1987-06-12 1988-06-10 Passage biosynthetique pour threonine de c. glutamicum WO1988009819A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6255287A 1987-06-12 1987-06-12
US062,552 1987-06-12

Publications (2)

Publication Number Publication Date
WO1988009819A2 WO1988009819A2 (fr) 1988-12-15
WO1988009819A3 true WO1988009819A3 (fr) 1989-03-09

Family

ID=22043247

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1988/002029 WO1988009819A2 (fr) 1987-06-12 1988-06-10 Passage biosynthetique pour threonine de c. glutamicum

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US (1) US5641660A (fr)
WO (1) WO1988009819A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5726299A (en) * 1991-06-03 1998-03-10 Mitsubishi Chemical Corporation Promoter DNA fragment from coryneform bacteria
US5693781A (en) * 1991-06-03 1997-12-02 Mitsubishi Chemical Corporation Promoter DNA fragment from coryneform bacteria
JP3783065B2 (ja) * 1994-03-04 2006-06-07 味の素株式会社 Ｌ−リジンの製造法
PL359863A1 (en) * 1999-06-25 2004-09-06 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding stress, resistance and tolerance proteins
WO2001000844A2 (fr) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Proteines codant pour les genes corynebacterium glutamicum, intervenant dans le metabolisme du carbone et dans la production d'energie
US6451564B1 (en) 1999-07-02 2002-09-17 Massachusetts Institute Of Technology Methods for producing L-isoleucine
US6962989B1 (en) * 1999-07-08 2005-11-08 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding novel proteins
DE19941478A1 (de) * 1999-09-01 2001-03-08 Degussa Neue für das thrE-Gen codierende Nukleotidsequenzen und Verfahren zur fermentativen Herstellung von L-Threonin mit coryneformen Bakterien
CN1303944A (zh) * 1999-10-27 2001-07-18 上海博容基因开发有限公司 一种新的多肽——苏氨酸合成酶71和编码这种多肽的多核苷酸
JP4623825B2 (ja) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
AU2001287631A1 (en) * 2000-08-02 2002-02-13 Degussa A.G. Nucleotide sequences which code for the metf gene
AU2002230431A1 (en) 2000-11-15 2002-05-27 Archer-Daniels-Midland Company Corynebacterium glutamicum promoters
US20050255568A1 (en) * 2003-05-30 2005-11-17 Bailey Richard B Methods and compositions for amino acid production
KR101481142B1 (ko) 2013-03-04 2015-01-15 한국과학기술원 코리네박테리아 발현용 합성프로모터
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
KR101996769B1 (ko) * 2018-12-21 2019-10-01 씨제이제일제당 (주) 변이형 호모세린 디하이드로게나제 및 이를 이용한 호모세린 또는 호모세린 유래 l-아미노산의 생산 방법

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4278765A (en) * 1978-06-30 1981-07-14 Debabov Vladimir G Method for preparing strains which produce aminoacids
EP0066129A2 (fr) * 1981-05-11 1982-12-08 Ajinomoto Co., Inc. Procédé de préparation de L-thréonine par fermentation
EP0088166A2 (fr) * 1981-12-29 1983-09-14 Kyowa Hakko Kogyo Co., Ltd. Procédé pour exprimer un gène
EP0179338A2 (fr) * 1984-10-04 1986-04-30 Ajinomoto Co., Inc. Procédé de préparation de L-aminoacides
EP0190921A2 (fr) * 1985-02-04 1986-08-13 Engenics, Inc. Procédé de surproduction d'acides aminés
EP0204326A2 (fr) * 1985-06-05 1986-12-10 Kyowa Hakko Kogyo Co., Ltd. Procédé de préparation de L-thréonine et L-isoleucine
EP0215388A1 (fr) * 1985-09-06 1987-03-25 Ajinomoto Co., Inc. Vecteur de plasmide et une méthode de régulation d'expression de gène par utilisation de ce vecteur
WO1987002984A1 (fr) * 1985-11-12 1987-05-21 American Biogenetics Corporation Cassette biogenetique
EP0233581A2 (fr) * 1986-02-12 1987-08-26 Kyowa Hakko Kogyo Co., Ltd. Procédé de production de L-thréonine ou de L-isoleucine

Family Cites Families (1)

* Cited by examiner, † Cited by third party
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US4894331A (en) * 1985-09-27 1990-01-16 Amgen Inc. Partial marker cassette mutagenesis of xylose isomerase

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4278765A (en) * 1978-06-30 1981-07-14 Debabov Vladimir G Method for preparing strains which produce aminoacids
EP0066129A2 (fr) * 1981-05-11 1982-12-08 Ajinomoto Co., Inc. Procédé de préparation de L-thréonine par fermentation
EP0088166A2 (fr) * 1981-12-29 1983-09-14 Kyowa Hakko Kogyo Co., Ltd. Procédé pour exprimer un gène
EP0179338A2 (fr) * 1984-10-04 1986-04-30 Ajinomoto Co., Inc. Procédé de préparation de L-aminoacides
EP0190921A2 (fr) * 1985-02-04 1986-08-13 Engenics, Inc. Procédé de surproduction d'acides aminés
EP0204326A2 (fr) * 1985-06-05 1986-12-10 Kyowa Hakko Kogyo Co., Ltd. Procédé de préparation de L-thréonine et L-isoleucine
EP0215388A1 (fr) * 1985-09-06 1987-03-25 Ajinomoto Co., Inc. Vecteur de plasmide et une méthode de régulation d'expression de gène par utilisation de ce vecteur
WO1987002984A1 (fr) * 1985-11-12 1987-05-21 American Biogenetics Corporation Cassette biogenetique
EP0233581A2 (fr) * 1986-02-12 1987-08-26 Kyowa Hakko Kogyo Co., Ltd. Procédé de production de L-thréonine ou de L-isoleucine

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Chemical Abstracts, volume 101, no. 21, 19 November 1984, (Columbus, Ohio, US), K. Yoshimura et al.: "Construction of a promoter-probe vector for a Bacillus subtilis host by using the trpD+ gene of Bacillus amyloliquefaciens" see page 176 *
Food Technology, volume 40, no. 10, October 1986, Institute of Food Technologists, (Chicago, Illinois, US), M.T. Follettie et al.: "Recombinant DNA technology for Corynebecterium glutamicum", pages 88-94 *
Journal of Biotechnology, volume 5, no. 4, May 1987, Elsevier Science Publishers B.V. (Biomedical Division), (Amsterdam, NL), Y. Morinaga et al.: "Expression of Escherichia coli promoters in Brevibacterium lactofermentum using the shuttle vector pEB003", pages 305-312 *

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US5641660A (en) 1997-06-24
WO1988009819A2 (fr) 1988-12-15

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