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WO1988007670A3 - Appareil de fluorescence a resolution dans le temps et immunoanalyse - Google Patents

Appareil de fluorescence a resolution dans le temps et immunoanalyse Download PDF

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Publication number
WO1988007670A3
WO1988007670A3 PCT/US1988/001019 US8801019W WO8807670A3 WO 1988007670 A3 WO1988007670 A3 WO 1988007670A3 US 8801019 W US8801019 W US 8801019W WO 8807670 A3 WO8807670 A3 WO 8807670A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescence
time
immunoassay
optical image
current
Prior art date
Application number
PCT/US1988/001019
Other languages
English (en)
Other versions
WO1988007670A2 (fr
Inventor
Yong-Sheng Chao
Salvador M Fernandez
Ernest F Guignon
Hann-Ching P Wang
Original Assignee
Chimerix Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/031,288 external-priority patent/US4855930A/en
Priority claimed from US07/031,408 external-priority patent/US4923819A/en
Application filed by Chimerix Corp filed Critical Chimerix Corp
Publication of WO1988007670A2 publication Critical patent/WO1988007670A2/fr
Publication of WO1988007670A3 publication Critical patent/WO1988007670A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/069—Supply of sources
    • G01N2201/0696—Pulsed
    • G01N2201/0697—Pulsed lasers

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Dans un procédé et un appareil de spectroscopie en fluorescence à résolution dans le temps, on utilise de la lumière laser provenant d'une seule impulsion pour exciter des photons fluorescents dans un échantillon (24), laquelle fluorescence est détectée par un tube photomultiplicateur (14) optimisé au niveau de la linéarité et du temps de réponse pour produire des photoélectrons qui génèrent un courant à l'anode du tube photomultiplicateur. Ce courant est déchargé à travers un réseau à résistance-capacité pour produire une forme d'onde de tension qui est convertie en une image optique (26), intensifiée (28), mémorisée (36) et numérisée (34). La version numérisée de l'image optique est traitée dans un processeur (38) en vue de calculer la réponse d'impulsion réelle en fluorescence. Est également décrite une immuno-analyse en fluorescence pour déterminer des analytes simples ou multiples sur la base d'une seule mesure de fluorescence.
PCT/US1988/001019 1987-03-27 1988-03-24 Appareil de fluorescence a resolution dans le temps et immunoanalyse WO1988007670A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US031,408 1987-03-27
US07/031,288 US4855930A (en) 1987-03-27 1987-03-27 Method and appartatus for improved time-resolved fluorescence spectroscopy
US07/031,408 US4923819A (en) 1987-03-27 1987-03-27 Time-resolved fluorescence immunoassay
US031,288 1987-03-27

Publications (2)

Publication Number Publication Date
WO1988007670A2 WO1988007670A2 (fr) 1988-10-06
WO1988007670A3 true WO1988007670A3 (fr) 1989-01-26

Family

ID=26707047

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1988/001019 WO1988007670A2 (fr) 1987-03-27 1988-03-24 Appareil de fluorescence a resolution dans le temps et immunoanalyse

Country Status (2)

Country Link
ES (1) ES2006611A6 (fr)
WO (1) WO1988007670A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2224352B (en) * 1988-10-05 1992-09-16 Hamamatsu Photonics Kk Light waveform measuring apparatus
FI82144C (fi) * 1989-03-22 1991-01-10 Wallac Oy Foerfarande foer samtidig bestaemning av flera ligander.
US4977325A (en) * 1989-07-12 1990-12-11 P B Diagnostic Systems, Inc. Optical read system and immunoassay method
CA2026257C (fr) * 1989-09-26 2001-07-24 Keiji Sasaki Dispositif d'analyse de formes d'onde transitoires
DE19718016A1 (de) * 1997-04-29 1998-11-05 Drexhage Karl Heinz Verfahren zur optischen Detektion von Analytmolekülen in einem natürlichen biologischen Medium
GB0001089D0 (en) 2000-01-18 2000-03-08 Council Cent Lab Res Councils Lipoprotein assay
DE10308814A1 (de) * 2003-02-27 2004-09-09 Chromeon Gmbh Bioanalytisches Verfahren auf Grundlage der Messung der Abklingzeit der Phosphoreszenz
RU2264610C2 (ru) * 2004-01-16 2005-11-20 Общество с ограниченной ответственностью "ВИНТЕЛ" Способ измерения спектроскопических свойств сыпучих продуктов и устройство для его осуществления
RU2253859C1 (ru) * 2004-08-25 2005-06-10 Государственное образовательное учреждение высшего профессионального образования "Оренбургский государственный университет" Способ контроля качества жидких смесей путем регистрации замедленной флуоресценции
WO2012012748A2 (fr) * 2010-07-23 2012-01-26 Luminex Corporation Co-couplage pour réguler la réactivité des réactifs dans des dosages immunologiques
JP6153951B2 (ja) 2012-03-16 2017-06-28 スタット−ダイアグノスティカ アンド イノベーション， エス． エル． 統合された移送モジュールを有する試験カートリッジ
CN103558433B (zh) * 2013-10-24 2016-03-30 北京无线电计量测试研究所 一种用于光电探测器冲激响应测量的装置及方法
CN103728446B (zh) * 2013-12-11 2016-02-24 天津大学 光子计数型多通道时间分辨荧光免疫分析系统及计数方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4368047A (en) * 1981-04-27 1983-01-11 University Of Utah Research Foundation Process for conducting fluorescence immunoassays without added labels and employing attenuated internal reflection
US4385126A (en) * 1980-11-19 1983-05-24 International Diagnostic Technology, Inc. Double tagged immunoassay
GB2129548A (en) * 1982-11-01 1984-05-16 Hamamatsu Corp Compact temporal spectral photometer
WO1986002734A1 (fr) * 1984-10-31 1986-05-09 Hyperion Catalysis International, Inc. Composes chelates metalliques de marquage luminescent et dispositif de detection
JPH06128844A (ja) * 1992-10-20 1994-05-10 Tsudakoma Corp 多色用レピア織機における不良緯糸分離装置

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4385126A (en) * 1980-11-19 1983-05-24 International Diagnostic Technology, Inc. Double tagged immunoassay
US4368047A (en) * 1981-04-27 1983-01-11 University Of Utah Research Foundation Process for conducting fluorescence immunoassays without added labels and employing attenuated internal reflection
GB2129548A (en) * 1982-11-01 1984-05-16 Hamamatsu Corp Compact temporal spectral photometer
WO1986002734A1 (fr) * 1984-10-31 1986-05-09 Hyperion Catalysis International, Inc. Composes chelates metalliques de marquage luminescent et dispositif de detection
JPH06128844A (ja) * 1992-10-20 1994-05-10 Tsudakoma Corp 多色用レピア織機における不良緯糸分離装置

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
1969 Wescon Technical Papers, Part 4 Instruments and Systems, Western Electronic Show and Convention, 19-22 August, 1969 (US) G.T. Longerbeam et al.: "High speed single transient oscilloscopes, the state of the art, and current potential for mating to on-line computers", pages 13/4: 1-10 *
Japanese Journal of Applied Physics, vol. 15, no. 5, 1976 (Tokyo, JP)H. Saito et al.: "A high sensitivity television camera system for pico-second spectroscopy", pages 947,948 *
Modern Fluorescence Spectroscopy, vol. 4, 1981, Plenum Press (New York, US) J.H. Richardson: "Applications of lasers in analytical molecular fluorescence spectroscopy", page 15 *
Patent Abstracts of Japan, vol. 10, no. 180 (P-471)(2236) 24 June 1986; & JP-A-6128844 (HAMAMATSU PHOTONICS K.K.) 8 February 1986 *
R.I. Sha'afi et al.: "Fast methods in physical biochemistry and cell biology", published 1983 by Elsevier (Amsterdam, NL) S.M. Fernandez: "Time-resolved fluorescence spectroscopy", pages 221-279 *
Review of Scientific Instruments, vol. 52, no. 11, November 1981 (New York, US) F. Docchio et al.: "Subnanosecond fluorescence waveforms measurements with a dual time-scale microprocessor-controlled averager", pages 1671-1675 *

Also Published As

Publication number Publication date
WO1988007670A2 (fr) 1988-10-06
ES2006611A6 (es) 1989-05-01

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